In vitro fertilization embryo transfer
-
Upload
niyamat-panjesha -
Category
Technology
-
view
1.112 -
download
3
description
Transcript of In vitro fertilization embryo transfer
![Page 1: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/1.jpg)
IN VITRO FERTILIZATION-EMBRYO TRANSFER (IVF-ET)
Presented by
NIYAMAT M. PANJESHA
A seminar on
Modern College Of Arts , Science & Commerce.Shivajinagar, Pune-05.
![Page 2: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/2.jpg)
WHAT IS IVF?
In vitro fertilization broadly deals with “the removal of egg’s from a women, fertilizing them in the laboratory, and transferring the fertilized eggs (zygotes) into the uterus a few days later.
![Page 3: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/3.jpg)
WHY IVF?
Infertility: Extremely Prevalent Definition: inability to achieve pregnancy 17-26% of couples worldwide (90 million
women) 27-47% of infertile couples have impaired
male fertility; decline in semen quality worldwide over last 50 years
![Page 4: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/4.jpg)
WHY THE INCREASE IN INFERTILITY?
Delayed marriage and child-bearing Sexually transmitted diseases Hormonal contraception Abortion Obesity Environmental Pollution
![Page 5: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/5.jpg)
Male infertility Female infertility
![Page 6: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/6.jpg)
IVF: HISTORY
In vitro fertilization (IVF) is a fertility procedure which first succeeded as recently as 1978 by Dr. Edwards ( an embryologist) and Dr. Steptoe ( a gynecologist) in England. Since then the technology has been further refined and developed by physicians and embryologists, with over 20,000 babies born worldwide.
![Page 7: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/7.jpg)
METHODOLOGY OF IVF
The ivf broadly involves the fowllowig stages:-
1. Ovarian stimulation and monitoring2. Egg (oocyte) retrieval3. Fertilization4. Intracytoplasmic Sperm Injection5. Embryo transfer
![Page 8: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/8.jpg)
STAGE 1: OVARIAN STIMULATION ANDMONITORING
Ovarian stimulation is used to produce multiple mature follicles, rather than the single egg normally developed each month.
![Page 9: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/9.jpg)
STAGE 2: EGG RETRIEVAL
Egg recovery is accomplished by a transvaginal procedure, which can be performed under light sedation or local anesthesia.
![Page 10: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/10.jpg)
NORMAL EGG ABNORMAL EGG
![Page 11: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/11.jpg)
STAGE 3: FERTILIZATION
Once mature eggs have been retrieved, the sperm and eggs are placed together in the laboratory and incubated in a meticulously prepared culture medium at a temperature identical to that of the woman’s body.
![Page 12: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/12.jpg)
STAGE 4: INTRACYTOPLASMIC SPERMINJECTION
During the process of ICSI, an embryologist isolates a sperm cell, draws it up into a microscopic needle and injects it inside an egg using a high power microscope.
![Page 13: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/13.jpg)
![Page 14: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/14.jpg)
STAGE 5: EMBRYO TRANSFER
Embryo transfer is not a painful procedure and can be performed without sedation.
Usually up to three embryos are placed together in a catheter ( a tubular instrument) and transferred, via the vagina, into the uterus.
![Page 15: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/15.jpg)
SUCCESS RATE OF IVF
Varies from programme to programme. It is dependent upon correct diagnosis
of patient, and age. Pregnancy rate is in the range of 25-
30% per oocyte retrieval. The take home is about 15-20% per
procedure.
![Page 16: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/16.jpg)
FACTORS
Increased risk of abortion. Multiple pregnancy. Ectopic pregnancy. Low birth weigh baby. Premature delivery.
![Page 17: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/17.jpg)
LIMITATION OF IVF
Genetic defect in oocytes. Genetic defect in fertilizing sperm. Environmental mutagenesis. Inadequate supply of nutrients and
hormones. Exposure to toxic agents and free
radicals.
![Page 18: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/18.jpg)
WORLDS FIRST TEST TUBE BABY
![Page 19: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/19.jpg)
REFERENCES
![Page 20: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/20.jpg)
ANY QUESTION
?
![Page 21: In vitro fertilization embryo transfer](https://reader034.fdocuments.us/reader034/viewer/2022042513/549c4d47b47959d4318b46e1/html5/thumbnails/21.jpg)
THANK YOU