Important Information - Sonidel

Important Information

i

Important Information

This product is authorized for laboratory research use only, not medical

product. The product has not been qualified or found safe and effective for

any human or animal diagnostic or therapeutic application. Uses for other

than the intended use may be a violation of applicable law.

The In Vitro High Voltage Electroporator ELEPO21 is an electroporation device designed to provide a

convenient way to introduce materials such as foreign DNA into sample’s cells, bacteria, fungi and

yeasts. This manual provides instructions for proper setup and operation of the ELEPO21 and its

components.

Safety Precautions

ii

Safety Precautions

Safety Terms and Symbols

Danger Disregarding instructions marked with this symbol may lead to serious injury

or death.

Warning Disregarding instructions marked with this symbol may lead to serious injury

or property damage.

Caution Disregarding instructions marked with this symbol may lead to injury or

property damage.

General cautions

Danger 1) Do not use outside the range of product specification. Comply with the

contents described in this instruction manual when using the product.

2) Do not modify or perform unnecessary disassembly. It may cause

electrocution to the user and malfunction or damage to the device.

Handling precautions

Danger

Do not touch the voltage output sockets on the device during the delivery of

the electric current, i.e., after pressing the start button or the foot switch.

To touch these parts during the delivery of an electric current is extremely

dangerous and will cause an electric shock.

Safety Precautions

iii

Warning 1) Do not open the cover of the product when the power is ON.

2) Do not touch the un-insulated parts of cables and electrodes when power

is on.

3) Do not operate this product in any of the following scenarios:

There is physical damage to the product.

Smoke, strange noise or fumes come from the product.

Contact your supplier immediately.

Location of warning labels

Caution The warning labels must always be legible. Ensure that the warning labels

are properly placed and legible. If a warning label is illegible or is peeling off,

contact us to replace it.

Power source, grounding and wiring cautions

Warning 1) To avoid the risk of fire, or electrical shock, observe all ratings and

markings on the product. Consult this manual for further ratings

information before making connections to the product.

2) This product is grounded through the grounding conductor of the power

cord. To avoid electric shock, the grounding conductor must be connected

to an earth grounding.

3) Use only the following power supply cord. Using the wrong power cord

could result in danger or fire.

・For AC100-120V area

UL Listed, detachable power cord set, 3 conductor grounding Type

SVT, No. 18 AWG, 3 m long maximum, rated at AC125V minimum.

・For AC220-240V area

Approved according to EU/EN standards, 3 conductor grounding

Type H05VV-F, 3 m long maximum, rated at AC250V minimum.

4) Do not bend the power cord forcibly or place heavy object on the cord

because it might damage the cord and cause fire or electric shock.

Safety Precautions

iv

Caution 1) Be sure to hold the connector part of the power cable when disconnecting

the cable. Pulling on the cord section may cause it to fray and break.

2) Disconnect power cable from the product if the product is not in use.

Usage environment precautions

Caution 1) Do not install or store the product in locations such as those listed below:

Locations subject to direct sunlight

Locations subject to strong impact or vibration

Location subject to high temperatures or high humidity

Locations that are submerged in water

2) Do not use this product with corrosive fluids. Avoid operation in humid or

corrosive environments.

3) Do not bring a strong magnet or magnetic field close to the main body of

the product.

4) Refer to installation instructions for details on installing the product to

ensure proper ventilation.

Table of Contents

v

Table of Contents

Important Information ............................................................................................. i

Safety Precautions ................................................................................................. ii

Table of Contents ................................................................................................... v

1 Checking the Package ................................................................................. 1-1

2 Related Products ......................................................................................... 2-1

3 Part Names ................................................................................................... 3-1

4 Installation and Connecting ........................................................................ 4-1

4.1 Installation........................................................................................................... 4-1

4.2 Connecting ......................................................................................................... 4-2

5 Operating Instructions................................................................................. 5-1

5.1 Overview ............................................................................................................. 5-1

5.2 Setting Electric Pulse Parameters ...................................................................... 5-1

5.2.1 Display in Selection window ............................................................ 5-1

5.2.2 Parameters of Poring Pulse mode .................................................. 5-2

5.2.3 Setting parameters of Poring Pulse mode ...................................... 5-2

5.2.4 Parameters of Transfer Pulse mode ............................................... 5-4

5.2.5 Setting parameters of Transfer Pulse mode ................................... 5-4

5.2.6 Setting current limit .......................................................................... 5-5

5.3 Saving, Copying and Recalling the Program...................................................... 5-6

5.4 Measuring Electrical Impedance ........................................................................ 5-8

5.5 Executing Program (Delivering Electric Pulses) ................................................. 5-8

5.6 Displaying Output Electric Pulse Parameters..................................................... 5-9

6 Additional Information ................................................................................. 6-1

6.1 Specifications ...................................................................................................... 6-1

6.2 Proper Selection of The Power Supply Cord ...................................................... 6-3

6.3 ELEPO21 Novel 2-Step Multiple Pulses Regime ............................................... 6-4

6.3.1 About pulse waveform .................................................................... 6-4

6.3.2 Poring Pulse and Transfer Pulse .................................................... 6-5

6.4 Warranty ............................................................................................................. 6-6

Table of Contents

vi

1 Checking the Package

1-1

1 Checking the Package

ELEPO21 Electroporator x 1

Accessories

Power cord x 1

Instruction Manual (this manual) x 1

2 Related Products

2-1

2 Related Products

CU500 Cuvette Chamber

The CU500 Cuvette Chamber can be connected to the ELEPO21 electroporator and is used for cell

transfection with NEPA electroporation cuvettes. Electric pulses from the ELEPO21 are delivered

through this product.

NEPA Electroporation Cuvettes

EC-001S, EC-002S, EC-004S, EC-001, EC-002 and EC-004

.

NEPA Electroporation Cuvettes are available in three gap widths: 0.4, 0.2, and 0.1 cm, to facilitate

delivery of the proper field strength to a variety of cell types.

Simply fill the cuvette with the sample using the transfer pipette and place the cuvette in the CU500

Cuvette Chamber.

Cat # Description Unit

EC-001S NEPA Electroporation Cuvettes with a pipette

1mm gap, 20～70µl (Brown color cap)

50/pkg


2mm gap, 40～4000µl (Green color cap)

50/pkg


4mm gap, 80～800µl (Blue color cap)

50/pkg

EC-001 NEPA Electroporation Cuvettes

1mm gap, 20～70µl (Brown color cap)

50/pkg


2mm gap, 40～4000µl (Green color cap)

50/pkg


4mm gap, 80～800µl (Blue color cap)

50/pkg

2 Related Products

2-2

3 Part Names

3-1

3 Part Names

Front

Rear

Power Inlet RS232C port

Cooling fan

Power Switch

Voltage Output

Control Panel

3 Part Names

3-2

Control Panel

1. Poring Pulse window

Set Parameters frame

A. Voltage [V] Display the set pulse voltage value.

B. Pulse Length [msec] Display the set pulse length in milliseconds.

C. Pulse Interval [msec] Display the pulse interval (duration between pulses) in milliseconds.

D. Number of Pulses Display the number of set pulses.

E. Polarity [+ / +/-] Display the set decay rate of pulses.

Measurements frame

F. Voltage [V] Display the measured output voltage value.

G. Current [A] Display the measured output current value.

H. Energy [J] Display the calculated output pulse energy in joules.

4

1.

2.

3

5.

A B C D E F G H

3 Part Names

3-3

2. Transfer Pulse window

Set Parameters frame

A. Voltage [V] Display the set pulse voltage value.

B. Pulse Length [msec] Display the set pulse length in milliseconds.

C. Pulse Interval [msec] Display the pulse interval (duration between pulses) in milliseconds.

D. Number of Pulses Display the number of set pulses.

E. Polarity [+ / +/-] Display the set electrical polarity (+ only or +, followed by -).

Measurements window

F. Voltage [V] Display the measured output voltage value.

G. Current [A] Display the measured output current value.

H. Energy [J] Display the calculated output pulse energy in joules.

3. Selection window

A. Poring Pulse frame Illuminate the editable parameter.

B. Transfer Pulse frame Illuminate the editable parameter.

C. Others frame Illuminate the editable item.

4. Impedance window

A. Impedance [kΩ] - Display the electrical impedance value between the electrodes by

pressing Ω button.

- Display the program number as “Ch.XX” when Memory in Others frame

in Selection window is selected and lighting up.

A B C D E G H

A B C

F

A

3 Part Names

3-4

5. Button Keys

A. Ω Execute the impedance measurement function between the electrodes

B. Data Send the data of the set and measured parameters.

C. Meas. Display the measured parameters.

D. Copy Copy the set parameters to another program number.

E. ◄ ▴ ▾ ▸ Select the parameter/item to be edited.

Change the displaying of Measurements.

F. Set Enable the edit of the parameter to be modified.

G. Start Execute the program (pulsing).

H. Stop/Clear Cancel the program or the parameter editing.

Delete the entered value.

I. Numerical keys Enter the numerical value.

J. Enter Save the entered value.

Execute the recall of the program.

A

B

C

D

E F

G

H

I

J

4 Installation and Connecting

4-1


4.1 Installation

Precautions for installation

Installation site

The ELEPO21 must be installed on a flat-level area.

Spacing for the cooling fan

Caution Be sure to leave a clearing of at least 20 cm behind the instrument for proper

ventilation.

4.2 Connecting

Precautions for wiring a power supply and grounding

Warning 1) Observe all ratings and markings on the product and confirm that they

correspond to the electrical conditions in the laboratory.

2) Use only the power cord specified for this product and certified for the

country of use. If no power cord is provided, please select the power cord

by referring to the section 6.2 Proper Selection of The Power Supply

Cord.

3) Connect the cable after confirming that the power switch is set to OFF.

4) Be sure to ensure that there is a protective earth before using the

instrument. Otherwise, there is a danger of electric shock. For safety

reasons and to minimize or remove unnecessary noise, be sure to ground

the instrument.

Grounding with the 3-pin plug

Insert the 3-core plug into a three-core outlet according to the following

figure

20cm


4-2

Precautions for wiring of electrodes

Warning Connect the caballing after confirming that the power switch is set at OFF.

Connecting Cuvette Chamber

1 ) Insert the male banana plugs of the CU500 Cuvette Chamber (optional) into the voltage output

slot located on the front panel of the ELEPO21.

2 ) Open the lid of the CU500 and then insert an electroporation cuvette (optional) into the insertion

slot of CU500.

5 Operating Instructions

5-1


5.1 Overview

The basic steps required to perform electroporation experiments using the ELEPO21 Electroporation

System are as follows:

5.2 Setting Electric Pulse Parameters

5.2.1 Display in Selection window

An indicator light corresponding to the selected parameter/item lights up. Select the parameter/item by

pressing ◄ ▴ ▾ ▸ buttons. The light corresponding to the selected parameter/item will change

accordingly.


5-2

5.2.2 Parameters of Poring Pulse mode

Voltage

The voltage of the first Poring Pulse (increments: 1V)

Pulse Length

The time duration of one Poring Pulse in milliseconds (increments: 0.1ms)

Pulse Interval

The time interval between Poring Pulses or the last Poring Pulse and the first Transfer Pulse in

milliseconds (increments: 0.1ms)

Number of Pulses

The number of Poring Pulses

Polarity Switching

This function reverses the polarity of Transfer Pulses. When the “+” indicator of the Polarity selection

option in the Set Parameters frame is illuminated, it means that the polarity switching function is OFF.

When the “+/-“indicator of the Polarity selection option is illuminated, it means that the polarity switching

function is ON. If the voltage, number of pulses are programmed, the out put pulses will be as follows;

5.2.3 Setting parameters of Poring Pulse mode

1 Use the ◄ ▴ ▾ ▸ buttons to select the desired parameter from within the Poring Pulse mode Selection

window.

2 When your choice is made: Press the Set button.

Time

Vo

ltag

e


5-3

3 Enter the desired value by pressing the numerical keys.

4 If you would like to cancel an entered value, press the Stop/Clear button.

5 Press the Enter button to save the desired value. When the value is saved, a short beep will sound

twice.

*If a value out-side of the specification range is selected and the Enter button is pressed to confirm the

selection, a long beep will sound three times and the previously selected value will be automatically

cancelled. Please re-enter a correct value within the specification range.

6 The entered values are displayed in the Set Parameters frame in the Poring Pulse window. When a

displayed value is blinking, it means that the value has not been saved yet.


5-4

5.2.4 Parameters of Transfer Pulse mode

Voltage

The voltage of the first Transfer Pulse (increments: 1V)

Pulse Length

The time duration of one Transfer Pulse in milliseconds (increments: 0.1ms)

Pulse Interval

The time interval between Transfer Pulses in milliseconds (increments: 0.1ms)

Number of Pulses

The number of Transfer Pulses

Polarity Switching

This function reverses the polarity of Transfer Pulses. When the “+” indicator of the Polarity selection

option in the Set Parameters frame is illuminated, it means that the polarity switching function is OFF.

When the “+/-“indicator of “the Polarity selection option is illuminated, it means that the polarity switching

function is ON. If the voltage, number of pulses and polarity switching (+/-) are programmed, the out put

Pulses will be as follows;

5.2.5 Setting parameters of Transfer Pulse mode

Setting Voltage, Pulse Length, Pulse Interval, Number of Pulses and Decay

Rate

The same as Poring Pulse mode. See “5.2.3. Setting parameters of Poring Pulse mode” on this manual.

Setting Polarity Switching

1 Select the Polarity Switching option in the Transfer Pulse frame in the Selection window by pressing ◄ ▴

▾ ▸ buttons.

Time

Vo

ltag

e


5-5

2 Press Set button to select “+” or “+/-“. Every time the Set button is pressed, “+” and”+/-“are alternated.

3 The status of the Polarity Switching function is displayed on Polarity indicator in the Set Parameters

frame in the Transfer Pulse window. The illuminated indicator indicates the selected option.

5.2.6 Setting Current Limit

Warning A current limitation function is automatically built into the ELEPO21.

Please ensure that the “On” selection is illuminated in the Current Limit frame

when the ELEPO21 is used for In Vivo, In Utero, and In Ovo or Ex Vivo

electroporation.

When the current limit function is selected, the maximum effective current value will be 2 Amperes.

For your safety, please ensure the “On” option is selected in the Current Limit frame in the Selection

window for in vivo, in utero, in ovo and ex vivo electroporation.

1 In the Others frame in the Selection window select the Current Limit option by pressing ◄ ▴ ▾ ▸

buttons.

2 Press the Set button to select On or Off. Each time the Set button is pressed, On and Off are alternated.

3 The status of the selected Current Limit is displayed in the Current Limit frame in the Selection window.

The illuminated indicator shows the selected setup item.


5-6

5.3 Saving, Copying and Recalling the Program

Saving the program

The values displayed in the Set Parameters frame in the Poring Pulse window and the Transfer Pulse

window are saved automatically on the current program number by pressing the Enter button.

*How to view the current program number.

1 Select the Memory option from within the Others frame in the Selection window by pressing ◄ ▴ ▾ ▸

buttons.

2 Then the number of the current program will be displayed in the Impedance window as “Ch.XX”.

Copying the program

The values of the parameters in the Set Parameters frame in the Poring Pulse window and the Transfer

Pulse window can be copied to another program number (1-99).

1 Select the Memory option in the Others frame in the Selection window by pressing ◄ ▴ ▾ ▸ buttons.

2 Press the Set button.


5-7

3 Enter the destination program number by pressing the numerical keys.

4 Press the Copy button. When the copying is completed, a short beep will sound twice.

Recalling the program

The saved program can be recalled.

1 Select the Memory option in the Others frame in the Selection window by pressing ◄ ▴ ▾ ▸ buttons.

2 Press the Set button.

3 Enter the desired program number by pressing the numerical keys.


5-8

4 Press the Enter button. When the program is successfully recalled, a short beep will sound twice.

5.4 Measuring Electrical Impedance

Electrical impedance between the electrodes can be measured. (unit: kΩ)

1 Press the Ω button.

2 The value of the measured impedance will be displayed in the Impedance window.

5.5 Executing Program (Delivering Electric Pulses)

Press the Start button in the Button Keys Selection to execute the program. Right after pressing Start

button, the electric pulses will be delivered.

During charging and pulsing, a warning beep is sounded.

When the pulsing is completed, a short beep will sound twice.


5-9

Danger Do not touch the exposed connections and components of electrodes and

cables when power is on and electric pulses are being delivered.

5.6 Displaying Output Electric Pulse Parameters

Default display

After delivering an electroporation program, the measured voltage and current of the first pulse and the

total energy of the pulses will be displayed in the Measurements frame in the Poring Pulse window and

the Transfer Pulse window.

In order to display the detailed parameters please follow to following procedures:

Displaying measured parameters of Poring Pulse

1 After completing the program, press the Meas. Button.

2 The values in the Measurements frame in the Poring Pulse window will blink. The blinking values show

the measured parameters of the first output Poring Pulse. At the same time, “1” indicating the first pulse

will be displayed on the Number of Pulses option within the Set Parameter frame in the Poring Pulse

window.

3 Press ▸ button to display the second Poring Pulse. The blinking values show the measured parameters

of the second output Poring Pulse. At the same time, “2” indicating the second pulse will be displayed on

the Number of Pulses option within the Set Parameter frame in the Poring Pulse window.


5-10

4 Press ▸ button to display the next output Poring Pulse. This process can be repeated until the last

pulse is displayed. The previous pulse can be displayed by pressing ◀ button.

5 The total energy of the output Poring Pulses will be displayed by pressing ▸ button after displaying the

last pulse. At the same time, “t” indicating total will be displayed on the Number of Pulses option within

the Set Parameter frame in the Poring Pulse window.

6 The averages of voltage, current and energy of the Poring Pulse will be displayed by pressing ▸ button

after displaying the total energy. At the same time, “A” indicating average will be displayed on the

Number of Pulses option within the Set Parameter frame in the Poring Pulse window.

Displaying measured parameters of Transfer Pulse

7 After applying an electroporation program, press the Meas. button, and then press ▾ button.


5-11

8 The values in the Measurements frame in the Transfer Pulse window will blink. The blinking values

show the measured parameters of the first output Transfer Pulse. At the same time, “1” indicating the

first pulse will be displayed on Number of Pulses option in the Set Parameter frame in the Transfer

Pulse window.

9 The steps to be followed are the same as those detailed above for displaying the Poring Pulse

measured output. See the step 3-6.

10 To change the display from Transfer Pulse to Poring Pulse, press ▴ button.

11 To finish the display, press the Stop/Clear button

6 Additional Information

6-1


6.1 Specifications

If no power supply cord is provided, please select the proper power supply cord for the ELEPO21 by

referring to “Specifications” and “Certified Cord” below:

Warning In case you use a non-approved power supply cord for Nepa Gene products,

Nepa Gene can no longer warrant the electrical safety of the device.

Poring Pulse

1 ) High Voltage Mode (automatic change)

Voltage 801 - 3,000V (increment: 1V)

Pulse Length 1,501 - 3,000V 0.1 - 5.0ms (increment: 0.1ms)

1,001 - 1,500V 0.1 - 10.0ms (increment: 0.1ms)

150.1 - 200.0V 0.1 - 50.0ms (increment: 0.1ms)

801 - 1,000V 0.1 - 99.9ms (increment: 0.1ms)

Pulse Interval 50 - 999.9ms (increment: 0.1ms)

Number of Pulses 0～9

Polarity Switching On: With polarity switching, OFF: Without polarity switching

Capacitance 55µF

2 ) Low Voltage Mode (automatic change)

Voltage 100 - 800V (increment: 1V)

Pulse Length 751 - 800V 0.1 - 15.0ms (increment: 0.1ms)

501 - 750V 0.1 - 20.0ms (increment: 0.1ms)

301 - 500V 0.1 - 30.0ms (increment: 0.1ms)

100 - 300V 0.1 - 50.0ms (increment: 0.1ms)

Pulse Interval 50 - 999.9ms (increment: 0.1ms)

Number of Pulses 0～9


Capacitance 995µF

Transfer Pulse

Voltage 1 - 300V (increment: 1V)

Pulse Length 101 - 300V 0.1 - 50.0ms (increment: 0.1ms)

1 - 100V 0.1 - 99.9ms (increment: 0.1ms)

Pulse Interval 50.0 - 999.9ms (increment: 0.1ms)


6-2

Number of Pulses 0-20


Capacitance 2,160µF

Output Measurements

Voltage Poring Pulse 1,000 - 3,276V (increment: 1V)

0.0 - 999.9V (increment: 1V)

Transfer Pulse 0.0 - 327.6V (increment: 0.1V)

Current Poring Pulse 10.00 - 99.99A (increment: 0.01A)

0.000 - 9.999A (increment: 0.001A)

Transfer Pulse 10.00 - 55.99A (increment: 0.01A)

0.000 - 9.999A (increment: 0.001A)

Energy Poring Pulse 1,000 - 9,999J (increment: 1J)

100.0 - 999.9J (increment: 0.1J)

10.00 - 99.99J (increment: 0.01J)

0.000 - 9.999J (increment: 0.001J)

*Joule(J) = Voltage x Ampere x Pulse length x Numbers of Pulses

Transfer Pulse 1,000 - 9,999J (increment: 1J)

100.0 - 999.9J (increment: 0.1J)

10.00 - 99.99J (increment: 0.01J)

0.000 - 9.999J (increment: 0.001J)

*Joule(J) = Voltage x Ampere x Pulse length x Numbers of Pulses

Others

Impedance Measurement 10.00k - 30.00kΩ (increment: 0.01kΩ)

0.030k - 9.999kΩ (increment: 0.001kΩ)

Memory 99 Programs

Pulse Current Limit Mode Over the current automatically shuts down, > 50A

Sample Resistance 30Ω minimum at 300V

Input Rating AC100-240V 50/60Hz

Operating Environment Temperature: 5-40℃,

Humidity: 85%RH Max (Non-condensing)

Dimensions 432(W) x 336(D) x 136(H) mm

Weight 8.7kg


6-3

6.2 Proper Selection of the Power Supply Cord

If no power supply cord is provided, please select the proper power supply cord for the ELEPO21 by

referring to “Specifications” and “Certified Cord” below:

Warning In case you use a non-approved power supply cord for Nepa Gene products,

Nepa Gene can no longer warrant the electrical safety of the device.

Specifications

Voltage Rating 125V AC (for 100-120V AC area) or 250V AC (for 220V AC area)

Current Rating 7A min. (for 100-120V AC area) 10A min. (for 220V AC area)

Temperature 60℃ min.

Length 3m max.

Fittings Configuration Grounding type attachment plug cap. Opposite terminals in molded-on

IEC Configuration appliance coupling.

Table 1 Certified Cord

A power supply cord should be certified by one of the agencies listed in Table 1, or comprised of

cordage marked with an agency marking per Table 1 or marked per Table 2. The fittings are to be

marked with at least one of agencies listed in Table 1. In case you are unable to buy locally in your

country the power supply cord which is approved by one of the agencies mentioned in Table 1, please

use replacements approved by any other equivalent and authorized agencies in your country.


6-4

Table 2 HAR Flexible Cord

Approval Organizations and Cordage Harmonization Marking Methods

6.3 ELEPO21 Novel 2-Step Multiple Pulses Regime

6.3.1 About pulse waveform

The ELEPO21 output pulse waveform from DC voltage is exponential-square. This ELEPO21

electroporation system makes it possible to set and apply an appropriate pulse voltage, pulse length

and pulse interval for transfection into each sample. This facilitates higher transfection efficiency and

higher sample viability without the need for or recourse to expensive disposable kits and special buffers.

For the low voltage transfection to suspension cells, the output pulse will be automatically decayed

because the impedance value is low (see left below figure). For the high voltage transfection to yeasts


6-5

and bacteria, the output pulse will be square shape without decay, because of its high impedance value

(see lower right figure).

6.3.2 Poring Pulse and Transfer Pulse

Electroporation is the application of pulsed electric fields to a sample to create temporary pores in the

sample’s cell membrane the purpose of which is to assist or eventuate the introduction of materials such

as foreign DNA into the sample’s cells (-similar to electrophoresis).

The transfection efficiency and the viability of the sample varies according to the parameters of the

delivered electric pulse. Multiple high voltage pulses with a short duration and with voltage decay

followed by multiple low pulses with a long duration and with voltage decay tends to cause lower

damage of the sample and to facilitate the delivery of significantly higher amounts of DNA into the

sample’s cells. The first (poring) pulses facilitate the formation of temporary pores and the second

(transferring) pulses facilitate the introduction of DNA into cells. Nepa Gene refers to the first pulse as a

“Poring Pulse” and the second pulse as a “Transfer Pulse” throughout this document.

In Poring Pulse mode, the voltage can be set up to 3,000V, the number of pulses can be set up to 9. In

Transfer Pulse mode, the voltage can be set from up to 99.9V, the number of pulses can be set up to 20

and the polarity exchange can be set on or off.

The optimal pulse parameters vary according to the application. Please contact Nepa Gene Co., Ltd. or

your local distributor for further information.

Time

Vo

ltag

e

-High Voltage -Short Duration

For Pore Formation with Low Damage

Poring Pulse

-Low Voltage -Long Duration

For DNA Transfer with Low Damage

Transfer Pulse

Time

Vo

ltag

e

Low Voltage Mode

Time

Vo

ltag

e

High Voltage Mode


6-6

Pulse parameters can be modified easily on the ELEPO21. Preliminary experiments may be needed

to optimize the parameters in order to achieve both high transfection efficiency and high cell viability. To

better understand the pulse parameters, please refer to the measured output values of voltage, current

and energy.

6.4 Warranty

Limited warranty for products

Nepa Gene Co., Ltd., warrants that its products, as delivered, shall conform to its specification and be

free of defects in materials and workmanship when used in accordance with their intended use. This

warranty is limited to twenty-four (24) months from the product’s original date of shipment from Nepa

Gene Co., Ltd. The warranty for non-durable products is limited to thirty (30) days from the product’s

original date of shipment from Nepa Gene Co., Ltd. Further, this warranty does not apply to

modifications made to the products by the customer, or damage to products resulting from modifications

or repairs effected by customer or other personnel not authorized by Nepa Gene Co., Ltd. to perform

such work. This warranty shall not apply to damage resulting from (I) loss or damage in transit, (II)

unreasonable use, (III) customer’s negligence, or (IV) accident. Nepa Gene Co., Ltd. reserves the right

to examine the alleged defective goods in order to determine whether the warranty is applicable.

Limited warranty and disclaimer

Nepa Gene Co., Ltd. warrants that all services supplied hereunder will be performed in a workmanlike

manner, except for the express warranties provided to customer under the above “limited warranty for

products”, Nepa Gene Co., Ltd. makes no other warranties or conditions, expressed or implied, as to the

services or parts supplied hereunder, and Nepa Gene Co., Ltd. expressly disclaims all warranties or

conditions of merchant-ability or fitness for a particular use.

Limitation of liability

Nepa Gene Co., Ltd. liability under this agreement is limited to the expense of providing product repair,

neither Nepa Gene Co., Ltd. nor its employees or agents shall be liable for indirect, special, incidental,

or consequential damages, including without limitation, business interruption, loss of profits or loss of

revenues, loss of wages or injury to persons, property or customers. Furthermore, Nepa Gene Co., Ltd.

categorically states that the sole and exclusive remedy for nonconforming goods shall be replacement

of defective goods or, at Nepa Gene’s option, refund of the purchase price to customer. The parties

acknowledge that the price of Nepa Gene Co., Ltd.’s products would be much greater if Nepa Gene Co.,

Ltd. undertook more extensive liability.

Any action by customer for any alleged breach of the warranty stated in this section must be brought to

the attention of Nepa Gene Co. Ltd. within 90 calendar days after the end of the warranty period.

Notice:

Products returned to Nepa Gene Co., Ltd. for repair or replacement shall be received prepaid.

If found not to be defective under the terms of warranty a charge will be made for repair or replacement

and freight costs will be at customer’s expense.

Specifications are subject to change without notice. For this reason and at its sole discretion, Nepa

Gene Co., Ltd. reserves the right to upgrade products during a repair process. Prices for out of warranty

repairs are subject to change without notice.

Contact

Area Distributor Address

Australia Berthold Australia Pty

Ltd

40 Clements Avenue, Bundoora, Victoria 3083, Australia

Phone: +61-3-9467-6277 Fax: +61-3-9467-7493

Canada

USA

Bulldog Bio, Inc. One New Hampshire Ave, Suite 125, Portsmouth, NH 03801, USA

Phone: +1-603-570-4248 Fax: +1-603-766-0524

Europe Sonidel Limited 32 Tonlegee Drive Cameron Park Raheny Dublin 5, Republic Ireland

Phone: +353-1-4434358 Fax: +353-1-6865462

Germany Xceltis GmbH Im Tal 12, 74909 Meckesheim, Germany

Phone: +49-6226-972418 Fax: +49-6226-972419

India Medi Analytika India

Pvt. Ltd

6, Adyar Bridge road, Adyar, Madras - 600 020, India

Phone: +91-44-2446 0988 Fax: +91-44-2446 3931

Israel Almog Diagnostic P.O Box 349, Shoham, 73142, Israel

Phone: +972-3-977-3390 Fax: +972-3-977-3391

Korea NEPABIO Co. #107-903, Raemian-Sukkwan, 407, Sukkwan-Dong, Sungbuk-Ku,

Seoul, 136-150, Korea

Phone: +82-10-6756-8620 Fax: +82-2-6442-7523

Korea Bio-Medical Science

Co., Ltd.

BMS Bldg., 22 Yeoksam-ro 7-gil, Gangnam-gu, Seoul 135-936,

Korea

Phone: +82-2-3471-6500 Fax: +82-2-3471-7001

Singapore Insta BioAnalytik Pte,

Ltd.

8 Boon Lay Way #03-10 TradeHub 21, 609964, Singapore

Phone: +65-6515-0110 Fax: +65-6515-0220

Taiwan Bio-East Technology

Co., Ltd.

9F., No.21 Fu-Kor Rd. Shi-Lin, Taipei, 111, Taiwan

Phone: +886-2-2831-3061 Fax: +886-2-2831-6374

Nepa Gene Co., Ltd.

3-1-6 Shioyaki, Ichikawa, Chiba, 272-0114, Japan

Phone: +81-(0)47-306-7222 Fax: +81-(0)47-306-7333

Email: [email protected]

ELEPO21 Instruction Manual

20150610

Important Information - Sonidel

Documents

Transcript of Important Information - Sonidel