Import of qPCR template (created by GENEIO) into the qPCR

instrument: SDS Software V2.1 – V2.4 (Applied Biosystems)

1. Log in

2. Click »File« � »New«

3. Leave settings as they appear (usually the block size of the instrument is already set

for the one you have in the lab) and click »OK«

4. Click »File« � »Import«

5. Browse for the qPCR template (output of GENEIO) in the folder where you saved it

(e.g. USB stick) and click »Import«

6. Result of the import: defined and assigned targets and samples

7. Click »Instrument« tab: check and under the tab »Thermal Cycler« manually set the

cycling conditions

8. Click »Real-Time« tab: connent software to the instrument insert the plate

(Open/Close) and »Start Run«

Export of qPCR results from the qPCR instrument: SDS Software

V2.1 – V2.4 (Applied Biosystems) for the import to GENEIO

1. Click »Analysis settings« button and select manual or automatic Threshold and

Baseline (according to the Guidelines from GENEIO)

2. Click on »Results« tab and select results of all or separate detectors (below) for which

one is going to define threshold and check the curves

3. Click »File« � »Export«

4. Leave selected »All wells«, select the folder where to save the results and click

»Export«. Import this file into GENEIO in the step Outputs&Inputs. GENEIO will

automatically ignore extra wells that do not belong to the specific experiment.