Impact of Ploidy of Yeast in Brewing

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The impact of ploidy of yeast on performance in brewing

FHM 30806Group 5: Mara Cabezas, Xianhan Guo, Timo Kramer, Eva Ramos, Birgit Scheepers, Sarah WeltjeIrma van Rijswijck & Rob Nout

Saccharomyces cerevisiae

Saccharomyces cerevisiae2Saccharomyces cerevisiae and beer

Different S. cerevisiae strains different beers

Ploidy of the yeast

Segregation

S. cerevisiae overviewIs used in brewing.With different strains we can obtain different beers.Can be either diploid or haploid. Research questionDoes yeast ploidy affect the brewing process?

Yeast variants used in this study4 strains

Mother strain

haploidA haploid

A diploid

Brew

Characterisation of the variantsEthanol resistanceGrowth kinetics(Temperature profiling, pH profiling)MorphologyMating test + qPCR(KEGG-mapping)

Impact of ploidy on performance in brewingTurbidityColourBitternessAroma profileGrowth kinetics

Questions?