Immunophenotyping: Myeloid cell maturation -...

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Hematopoietic stem cell Erythroblast CD71 Myeloblast Megakaryocyte CD61 Neutrophil CD15 CD16 Stem cell and early progenitor cells CD133, CD271, CD11 Stem cell, early and late progenitor cells CD347 Basophil CD123 Eosinophil CD44 Platelets CD61 Erythrocyte Monocyte CD14 CD11b Macrophage Promonocyte MDC1 CD1c Mast cell CD117 MDC2 CD141 Myeloid dendritic cells Myeloid progenitor cell CD33 DC1 DC2 Eosinophil E i hil Macrophage Macrophage Blood Bone Marrow Tissue Copyright © 2012 Abcam, All Rights Reserved. Immunophenotyping: Myeloid cell maturation Discover more at abcam.com/myeloid

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Hematopoieticstem cell

ErythroblastCD71

MyeloblastMegakaryocyteCD61

NeutrophilCD15CD16

Stem cell and early progenitor cells CD133, CD271, CD11

Stem cell, early and late progenitor cells CD347

BasophilCD123

EosinophilCD44

PlateletsCD61

Erythrocyte MonocyteCD14CD11b Macrophage

Promonocyte

MDC1CD1c

Mast cellCD117

MDC2CD141

Myeloiddendritic cells

Myeloidprogenitor cell

CD33

DC1 DC2

EosinophilE i hil

p gp gMacrophageMacrophageBlood

Bone Marrow

Tissue

Copyright © 2012 Abcam, All Rights Reserved.

Immunophenotyping: Myeloid cell maturation

Discover more at abcam.com/myeloid

276_12_AK Myeloid Cell Maturation Card:A4 14/05/2012 10:11 Page 1

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276_12_AK

Myeloid Lineage products from Abcam

Discover more at abcam.com/myeloid

­Featured products

­Target Alternative name Polyclonal Monoclonal Protein/ Unconjugated FITC PE APC peptide

Stem and progenitor cells

CD34 - ab63985 ab8158 ab18227 ab18228 ab18225 ab90823

CD117 c-Kit ab5506 ab111033 ab24870 ab111245 ab95678 ab101088

CD271 LNGFR ab38335 ab86768 ab62122 - - ab38334

Myeloid progenitor

CD33 - ab13963 ab2579 ab25773 - ab118967 -

Erythroblast

CD71 Transferrin receptor ab84036 ab1086 ab47095 ab18242 ab64672 ab101219

Megakaryocyte and Platelet

CD61 Integrin beta 3 ab47584 ab7167 ab92393 ab91128 ab91134 -

Granulocytes: Basophil

CD123 IL3RA ab53698 ab21562 ab95782 ab93523 ab95781 ab88358

Granulocytes: Eosinophil

CD44 - ab41478 ab6124 ab19622 ab58754 ab81424 ab41477

Granulocytes: Neutrophil

CD15 - - ab754 ab18272 ab64603 ab88032 ab114288

CD16 - ab94773 ab664 ab69761 ab18234 ab55864 -

Monocyte

CD11b - ab27537 ab1046 ab18273 ab28101 ab25482 -

CD14 - ab45870 ab760 ab28061 ab18271 ab60901 ab82279

Myeloid Dendritic cells: MDC1

CD1c - - ab18216 ab95757 - - ab114935

Myeloid Dendritic cells: MDC2

CD141 Thrombomodulin ab94373 ab6980 ab27396 - - ab106425

CD15 [MY-1] (ab754)

Clonality Applications Host Species cross reactivity

M IHC-Fr, IHC-P Ms Hu

CD44 [IM7] (ab19622)

Clonality Applications Host Species cross reactivity

M ICC/IF, IHC-P, Flow Cyt, IP, IHC-Fr Rt Hu

CD14 ELISA (ab46541)

Sample type Assay type Sensitivity Species cross reactivity

Cell culture supernatant, Sandwich < 6 ng/ml HumanSerum, Plasma

View more CD marker ELISA kits at: www.abcam.com/ELISA

DRAQ5™

Region Sample Excitation Emmission Applicationsof staining type maximum maximum

Nuclear DNA Fixed or non-fixed/ 646 nm 681 nm/ 697 nm Flow Cyt, ICC/IF live cells

CD71 (ab84036)

Clonality Applications Host Species cross reactivity

P WB, ICC/IF Rb Hu, Ms

MERGED

DRAQ5 ab7291- alpha Tubulin

ab16048 - Lamin B1

276_12_AK Myeloid Cell Maturation Card:A4 14/05/2012 10:11 Page 2