Immunohistochemistry Detection Systems€¦ · IHC Detection Systems | [email protected]...

www.biosb.com | [email protected] | [email protected]

Immunohistochemistry Detection SystemsImmunoDetector | PolyDetector | PolyDetector Plus

Bio SB Inc, 69 Santa Felicia Drive, Santa Barbara, CA 93117, USATel (USA): 1-800-561-1145 | Tel (Int): +1-805-692-2768 | Fax: 805-692-2769

A wide array of highly sensitive biotin & micro-polymer based detection systems.

For use in clinical and research laboratories.

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IHC Detection Systems

www.biosb.com | [email protected]

Bio SB offers the molecular pathology community a robust and diverse array of biotin and micro-polymer based detection systems for use in both research and clinical laboratories. With a variety of biotin/streptavidin & micro-polymer detection chemistries, Bio SB is sure to have a detection system to meet your needs. Paired with the large array of Bio SB antibodies, chromogens, and ancillaries, Bio SB offers laboratories a sensitive and versatile catalog of IHC products at a competitive price.

Bio SB IHC Detection Systems


Since its inception in Santa Barbara, California 15 years ago, Bio SB has developed an in-depth knowledge of immunohistochemical research, production, and manufacturing. Bio SB has produced high quality antibodies, ancillaries and detection chemistries since 1998 in an ISO 13485:2003 and FDA cGMP 820 approved facility. The mission of Bio SB is provide high quality & cost effective immunochemicals to researchers, clinicians, and pathologists globally.

About Bio SB

Presentation All Bio SB Detection systems are manufactured according to FDA and ISO guidelines to ensure a high quality In-vitro diagnostic kit. All Bio SB detection systems come complete with an endogenous peroxidase blocker, biotin/polymer linker (if applicable), AP or HRP labeled strepatividin or micro-polymer, and chromogen of choice.

Bio SB is headquartered in Santa Barbara, California 5 minutes from UCSB. Bio SB is an FDA/ISO 13485 certified facility.


Peroxidase Block1

1

Biotin or Polymer Link (if applicable)

2

2

Label3

3

4 Substrate Chromogen4

3 mL Tinto Predilute (30 tests)7 mL Tinto Predilute (70 tests)

15 mL Tinto Predilute (150 tests)• Cost effective solution.• Compatible with biotin

or micro-polymer based detection systems.

• Can be optimized to meet the needs of each laboratory.

• No need for antibody titering or optimization.

• Compatible with biotin or micro-polymer based detection systems.

0.1 mL Concentrate0.5 mL Concentrate

1 mL Concentrate

Bio SB Antibodies

Tinto Prediluted Antibodies Concentrated Antibodies

Compliment your Bio SB Detection chemistry with a selection of over 330 IVD and ASR antibodies from Bio SB. All antibodies are available in concentrate or prediluted format and validated for use with Bio SB detection systems.

Tinto Prediluted antibodies & control slides3mL, 7mL, and 15mL presentations



ImmunoDetector

Bio SB ImmunoDetector Overview

• Available in HRP or AP configurations.• Biotin-streptavidin immunohistochemistry detection technology.• Universal ready-to-use formulation detects mouse or rabbit antibodies .• Optimized for use with Bio SB Tinto Prediluted antibodies.• Kits available in mouse/rabbit configurations. • All kits manufactured according to US FDA and ISO 13485 Guidelines.

Presentations & Volumes

Catalog # Volume

BSB 0001 15 mL

BSB 0003 50 mL

BSB 0005 100 mL

BSB 0007 200 mL

BSB 0009 1000 mL

ImmunoDetector HRP/DAB

Catalog # Vol

BSB 0002 15 mL

BSB 0004 50 mL

BSB 0006 100 mL

BSB 0008 200 mL

BSB 0010 1000 mL

Recommended DAB Protocol

Deparaffinize Block Antibody Link Label DAB Hematoxylin

ImmunoDetector HRP/AEC

Catalog # Vol

BSB 0082 15 mL

BSB 0083 50 mL

BSB 0084 100 mL

BSB 0085 200 mL

BSB 0086 1000 mL

ImmunoDetector AP

Carbonic Anhydrase/DAB CD3 Epsilon/AEC CK34BE12/Alk Blue

ImmunoWasher

5 min. 30-60 min. 10 min. 10 min. 5-10 min. 30 secondsCK 14 RMab DAB Protocol Time

60 minutes

Bio SB ImmunoDetector Technology

The Bio SB ImmunoDetector is a highly sensitive biotin-streptavidin detection system that allows for the demonstration of antigens in paraffin-embedded tissue, cryostat sections, cytosmears, and cell preparations. The increased sensitivity of the ImmunoDetector Detection System allows for rapid staining procedures without compromising the quality of stains.

The ImmunoDetector Detection System is a universal detection system which is suitable for use with mouse or rabbit primary antibodies. Compared with competing avidin-biotin based detection systems, the immunodetector generates higher sensitivity and specificity at a competitive price.

1 2 3 4 5

5

Primary Antibody

Streptavidin HRP/AP

SubstrateChromogen

Biotin LinkAnti-M/R

Antigen

YY

2

PeroxidaseBlock

3

4

1

Above: Bio SB ImmunoDetector mechanism of action.

ImmunoWasher ImmunoWasher ImmunoWasher ImmunoWasher ImmunoWasher

6



PolyDetector

PolyDetector HRP/AP Overview

• Anti-mouse and rabbit Fab’ micro-polymer detection technology allows for better cell penetration to deliver a highly specific and sensitive signal.

• Available in HRP or AP configurations.• Universal Ready-to-use formulation detects mouse or rabbit antibodies .• AP and HRP Systems can be used in multiplex IHC staining.• Optimized for use with Bio SB Tinto Prediluted antibodies.• Kits available in mouse/rabbit configurations.• All kits manufactured according to US FDA and ISO 13485 Guidelines.


Catalog # Vol

BSB 0201 15 mL

BSB 0203 50 mL

BSB 0205 100 mL

BSB 0207 200 mL

BSB 0207A 1000 mL

Catalog # Vol

BSB 0282 15 mL

BSB 0283 50 mL

BSB 0284 100 mL

BSB 0285 200 mL

BSB 0286 1000 mL

PolyDetector HRP/DAB PolyDetector AP Kits


Actin SM/HRP Green SOX-2/Alk Magenta

Catalog # Vol

BSB 0202 15 mL

BSB 0204 50 mL

BSB 0206 100 mL

BSB 0208 200 mL

BSB 0208A 1000 mL

PolyDetector HRP/AEC

S-100/AEC

PolyDetector Plus HRP Overview


PolyDetector Technology The Bio SB PolyDetector is a non-biotin, micro-polymeric detection system that allows for the demonstration of antigens in formalin-fixed paraffin-embedded tissue, cryostat sections, blood smears, cytosmears, and cell preparations. The Bio SB PolyDetector was developed with proprietary tandem hyperlabelling micro-polymer technology used to directly label immunoglobulins with enzymes. This ensures consistent and reproducible immunostaining for all types of nuclear, cytoplasmic and membranous antigens, in different types of tissues. The increased sensitivity of the PolyDetector system allows for rapid staining procedures without compromising stain quality. Available in HRP and AP configurations, the PolyDetector is ideal for users with a need to implement multiplex IHC technologies into their laboratories.

Deparaffinize Block Antibody Label DAB Hematoxylin5 min. 30-60 min. 30-45 min. 5-10 min. 30 seconds

GLUT-1 RMab DAB Protocol Time85 minutes

Primary Antibody

AP/HRPMicro-polymer

Substrate Chromogen

Antigen

Y

2

PeroxidaseBlock

3

1

4

1 2 3 4

Above: Bio SB PolyDetector mechanism of action.

5

ImmunoWasher ImmunoWasher ImmunoWasher ImmunoWasher ImmunoWasher


Y



PolyDetector Plus HRP Overview

• Anti-mouse and rabbit Fab’ micro-polymer detection technology allows for better cell penetration to deliver a highly specific and sensitive signal.

• Universal Ready-to-use formulation detects mouse or rabbit antibodies .• Short incubation times for each polymer.• Optimized for use with Bio SB Tinto Prediluted antibodies.• Kits available in mouse/rabbit configurations.• All kits manufactured according to US FDA and ISO 13485 Guidelines.


Catalog # Vol

BSB 0257 15 mL

BSB 0259 50 mL

BSB 0261 100 mL

BSB 0263 200 mL

BSB 0265 1000 mL

Catalog # Vol

BSB 0258 15 mL

BSB 0260 50 mL

BSB 0262 100 mL

BSB 0264 200 mL

BSB 0266 1000 mL

PolyDetector Plus HRP/DAB PolyDetector Plus HRP/AEC


PolyDetector Plus HRP Technology

HER-2 RMab/DAB p40 RMab/DAB S-100 Beta & AECKi-67/AEC

PolyDetector Plus HRP

The Bio SB PolyDetector Plus system is a highly sensitive micro-polymeric detection system that allows for the demonstration of antigens in formalin-fixed paraffin-embedded tissue, cryostat sections, blood smears, cytosmears, and cell preparations. PolyDetector Plus is developed using a proprietary tandem hyperlabelling micro-polymer technology used to directly label immunoglobulins with enzymes which ensures consistent and reproducible immunostaining in different types of tissues. The PolyDetector Plus kit incorporates a linking polymer and a micro-polymer label. This multiple component polymer delivers a highly sensitive and specific signal in a shorter time-frame than the PolyDetector.

Deparaffinize Block Antibody Link Label DAB Hematoxylin5 min. 30-60 min. 15 min. 15 min. 5-10 min. 30 seconds

MSH 2 RMab DAB Protocol Time70 minutes

Primary Antibody

HRPMicro-polymer

Substrate Chromogen

Antigen

Y

2

PeroxidaseBlock

4

1

5

Link3Y

1 2 3 4 5

Above: Bio SB PolyDetector Plus mechanism of action.


6

ImmunoWasher ImmunoWasher ImmunoWasher ImmunoWasher ImmunoWasher ImmunoWasher



IHC Chromogens Bio SB Chromogens

DAB

Catalog # Vol

BSB 0015 15 mL

BSB 0016 50 mL

BSB 0017 100 mL

BSB 0018 200 mL

BSB 0018B 1000 mL

AEC

Catalog # Vol

BSB 0011 15 mL

BSB 0012 50 mL

BSB 0013 100 mL

BSB 0014 200 mL

BSB 0061 1000 mL

HRP Black

Catalog # Vol

BSB 0087 15 mL

BSB 0088 50 mL

BSB 0089 100 mL

BSB 0090A 200 mL

BSB 0090C 1000 mL

HRP Green

Catalog # Vol

BSB 0128 15 mL

BSB 0129 50 mL

BSB 0130 100 mL

BSB 0131 200 mL

BSB 0133 1000 mL

Alk Blue

Catalog # Vol

BSB 0062 15 mL

BSB 0063 50 mL

BSB 0064 100 mL

BSB 0065 200 mL

BSB 0066 1000 mL

Alk Red

Catalog # Vol

BSB 0067 15 mL

BSB 0068 50 mL

BSB 0069 100 mL

BSB 0070 200 mL

BSB 0071 1000 mL

Alk Magenta

Catalog # Vol

BSB 0077 15 mL

BSB 0078 50 mL

BSB 0079 100 mL

BSB 0080 200 mL

BSB 0081 1000 mL

Alk Brown

Catalog # Vol

BSB 0072 15 mL

BSB 0073 50 mL

BSB 0074 100 mL

BSB 0075 200 mL

BSB 0076 1000 mL

Bio SB substrate-chromogens are highly sensitive chromogens that are optimized for use in immunohistochemistry. Ideal for both clinical and research settings, Bio SB chromogens can be paired with either biotin or micro-polymer based detection systems. Multiplex IHC users can pair Bio SB AP & HRP PolyDetector systems with any combination of AP & HRP substrate-chromogens to detect multiple antigens on a tissue.

2 component chromogen

Ready to use



Ready to use

Ready to use


Ready to use

HRP Chromogens AP Chromogens

EGFR Phospho HER-2

MUC5AC

Actin S.M. Chromogranin A

INI-1CK 34BE12

ER

MM0002, Rev. A, DCN: 1906, 05/11/2015

Immunohistochemistry Detection Systems€¦ · IHC Detection Systems | [email protected]...

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