Innovative Peptide Solutions

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Immuno Tools for> Biomarker Discovery

> Seroscreening

> B- and T-cell Epitope Identification and Validation

> T-cell Assays

> Vaccine Development

> Antibody Signature Profiling

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HistoryJPT Peptide Technologies is a service provider based in Berlin, Germany and has achieved world-wide credibility for its commitment to rigorous quality standards and a reputation for developing and implementing innovative peptide based services and research tools for immunological and proteomic applications.

Together with its US-subsidiary, based in Acton near Boston, Massachusetts, JPT is serving a worldwide customer base in the pharmaceutical and biotechnology industries as well as researchers in universities, governmental and non-profit organizations.

Technology & ApplicationOver the past decade, JPT has developed a portfolio of proprietary technologies and developed a series of unique products and services which support research efforts in proteomics and in all developmental phases of novel vaccines against infectious and autoimmune diseases as well as against allergies and cancer.

In addition, JPT’s technologies find application in areas where peptide screening is needed. Typical examples are: screening for proteoptypic peptides, enzymatic activities (proteases, kinases, phosphatases, methyltransferases), or systematic optimization of peptide lead structures.

Quality AssuranceJPT is DIN EN ISO 9001:2008 certified and GCLP audited.

JPT’s proprietary key technologies are:

SPOT

High throughput peptide synthesisand screening technology forpeptides & peptidomimetics forT-cell epitope and peptide leaddiscovery and characterization on proteome wide levels.

PepStar™

Proprietary technology platformfor humoral immune responses for biomarker discovery, sero-screening and immune monitoring.

PepMix™

Defined antigen spanning peptide pools to stimulate CD4+ and CD8+ T-cell responses.

PepTrack™

Peptide libraries of individual peptides optimized for T-cell assays.

SpikeTides™

Small scale light and heavy labeled peptides for proteomics.

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ntsCellular immune responses

02 / PepTrack™ Peptide Libraries Individual peptides for specific requirements of T-cell assays

06 / PepMix™ Peptide Pools Antigen spanning or mixed epitope pools for immune monitoring,

vaccine efficacy testing & adoptive immunotherapy

08 / Custom Peptides ISO 9001:2008 certified synthesis for provision of peptides

fulfilling highest quality requirements

10 / Micro-Scale Peptides The most inexpensive source of small scale peptides on the market

Humoral immune responses 12 / PepStar™ Customized high density peptide microarrays for seromarker

discovery and immune monitoring

14 / PepSpots™ Customized cellulose bound peptide arrays for epitope mapping

16 / Epitope Discovery and Antibody Profiling Service Full incubation and data evaluation service with our peptide arrays

using purified biologics or patient samples

18 / BioTides™ High throughput assembly of biotinylated peptides for ELISA develop-

ment, microarray generation and more

19 / Bioinformatics & Cheminformatics Comprehensive bio- & cheminformatic services supporting peptide

library and array design for biomarker discovery, validation and optimization programs

20 / RepliTope™ Predefined peptide microarrays for mapping of immunodominant

regions

Our PepTrack™ options Research, Discovery and Trial Track are delivered in 96-tube racks for individual tube handling.

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s PepTrack™ Peptide Libraries

Custom peptide libraries optimized for performance in T-cell assays such as ELISPOT, CFC, etc.

Applications for PepTrack™

• T-cell epitope discovery • Peptide vaccine development • T-cell epitope mapping and validation • Immune monitoring • Optimization and validation of T-cell assays • Vaccine efficacy testing • Cell therapy approaches • Biological screening • Identification of proteotypic peptides

Benefits of PepTrack™

• Peptide specifications tailored for cell based assay applications

• Best pricing in industry starts at 7 $/€ per peptide • All production performed in an regulated environ-

ment according to DIN EN ISO 9001:2008 & GCLP standards; Audits welcome!

• Peptide protocols designed to avoid toxic contaminants

• Special synthesis technology inhibits side products causing false positive T-cell responses

• Provision of freeze dried aliquots for enhanced stability, validated pooling & vialing available

• Proven track record for applications in clinical studies

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What is PepTrack™?

JPT Peptide Technologies is the leading provider of peptide libraries for T-cell assays and pro-teomic applications worldwide. Together with its partners in the vaccine industry, governmen-tal and non-profit organisations JPT defined specific requirements for peptides to be used for T-cell assays in the various phases of vaccine development. These specifications were translated to a product line called PepTrack™ Peptide Libraries offering tailor made solutions for all peptide needs in vaccine development. Using its regulated environment (DIN EN ISO 9001:2008, GCLP) JPT produces more individual peptides on an annual basis than any other company worldwide.

PepTrack™ Options

Fast Track

Synthesized by JPT’s proprietary SPOT synthesis method, Fast Track Peptide Libraries represent the most inexpensive source of peptides worldwide. The ability to produce tens-of-thousands of pep-tides within weeks enables T-cell epitope discovery, biological screening and identification of pro-teotypic peptides at proteome-wide levels.

Research TrackResearch Track Peptide Libraries are designed for applications in T-cell assays requiring large num-bers of fully analyzed peptides in quantities of 1-5mg. All peptides are fully analyzed for identity and purity by LC-MS (no MALDI-MS!). In addition, JPT’s peptide synthesis protocols are optimized to avoid contaminations and failure sequences which cause either false positive T-cell responses or toxic inhibition.

Purity QA/QC* Length Scale Delivery Price/peptide

Unpurified - no purity guarantee 5% LC-MS 7-15aa 50 - 250nmol 2 weeks from 7 $/€

* additional QC available upon request Delivery Format: 96-well plate format

Purity QA/QC Length Scale* Delivery Price/peptide

Unpurified - no purity guarantee LC-MS 7-15aa 1-5mg 2 weeks from 25 $/€

main product= target peptide LC-MS 7-15aa 1-5mg 2 weeks please inquire

* for larger amounts please inquireDelivery Format: 96-array tube format

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Discovery Track Peptide Libraries have defined purities for applications requiring similar and specified purities of all peptides (i.e. immune monitoring). Each peptide has a guaranteed mini-mum specification. All peptides will be QC’d by LC-MS (not MALDI-MS!).

Trial Track Trial Track Peptide Libraries were designed to provide a reliable and competitive source of pep-tides and peptide pools for application as monitoring tools in clinical vaccination trials. All peptides have guaranteed purities according to specifications.

Purity QA/QC Length Scale* Delivery Price/peptide

> 70 % LC-MS 7-15aa 1-5mg 3 weeks from 45 $/€

* for larger amounts please inquireDelivery Format: 96-array tube format

Purity QA/QC* Length Scale** Delivery Price/peptide

> 80 % LC-MS 7-15 aa > 1 mg 2 weeks from 69 $/€

> 90 % LC-MS 7-15 aa > 1 mg 3 weeks please inquire

> 95 % LC-MS 7-15 aa > 1 mg 4 weeks please inquire

> 97 % LC-MS 7-15 aa > 1 mg 5 weeks please inquire

* full analytical coverage including amino acids analysis, capillary electrophoresis, peptide

content determination, determination of residual solvents etc. is available upon request ** for larger amounts please inquireDelivery Format: 96-array tube format

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Selected References> "Extensive Major Histocompatibility Complex

Class I Binding Promiscuity for Mycobacterium Tuberculosis TB10.4 Peptides and Immune Dominance of Human Leucocyte Antigen (HLA)-B*0702 and HLA-B*0801 Alleles in TB10.4 CD8+ T-Cell Responses"

Axelsson-Robertson et al. Immunology (2009)

> “Structural Basis for the Immunogenic Properties of the Meningococcal Vaccine Candidate LP2086”

Mascioni et al. The Journal of Biological Chemistry (2009)

> “Comprehensive Analysis of the α-Fetoprotein-Specific CD8+ T Cell Responses in Patients with Hepatocellular Carcinoma”

Thimme, Neagu, et al. Hepatology (2008)

> ”Identification and Functional Validation of MHC Class I Epitopes in the Tumor-Associated Antigen 5T4”

Shingler et al. International Immunology (2008)

> ”Acquisition of Direct Antiviral Effector Functions by CMV-Specific CD4+T Lymphocytes with Cellular Maturation”

Casazza et al. J. Exp. Med. (2006)

> “Design and Use of Conditional MHC Class I Ligands”

Toebes et al. Nat. Med. (2006)

> ”Novel Method for Detection of Virus-Specific CD4+ T Cells Indicates a Decreased EBV-Specific CD4+ T Cell Response in Untreated HIV-Infected Subjects”

Piriou et al. Eur. J. Immunol. (2005)

“I have been collaborating with JPT for several years. Their unique peptide library technologies and knowledge of specific peptide requirements for T-cell assays made them a valuable, long-term partner in our efforts to develop novel adoptive immunotherapy approaches to prevent and treat viral infections post-transplant as well as for the treatment of virus and non virus-associated malignancies. Furthermore, their reagents allow us to precisely monitor and track specific T-cell populations post-infusion.”Ann Leen, PhD, Baylor College of Medicine, Center for Cell and Gene Therapy, Houston, TX, USA

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PepMix™ peptide pools will be provided as freeze dried aliquots for highest shelf-stability and with full QC/QA documentation and application protocol.

Validated pooling and aliquoting is performed in an environ-ment regulated by ISO 9001:2008 and GCLP.

Applications for PepMix™

• Immunostimulation of T-lymphocytes • Monitoring of immune status during

diseases & therapeutic interventions• Assessment of vaccine efficacy

What is a PepMix™?Antigenic protein sequences will be divided into overlapping peptides, chemically synthesized and analyzed to comply with the requirements of T-cell assays. Pooling is performed using validated proto-cols ensuring the presence of all peptides in the respective pools. Our fully automated aliquotation service yields freeze dried pool aliquots minimizing deterioration of peptides that easily occurs in DMSO stock solutions.

> Peptide scan format: length 15 amino acids with overlap 11 amino acids

> Pool aliquots: 25 tests per vial (25 µg / peptide)

Benefits of PepMix™

• Simultaneous detection of CD4+ and CD8+ responses in a single sample

• Similar T-cell responses compared to whole protein antigens

• Longer shelf life and batch to batch reproducibility • Improved responses in stored blood and PBM cells

compared to whole protein antigens

Selection of available PepMixes™

Positive & negative control pools for T-cell assays: CMV (pp65, IE-1, IE-2 …) Infectious Diseases: Influenza (HA…);

BKV (VP1, large T...); EBV (EBNA1, BLZF1...); HadV-5 and HadV-3; Yellow Fever (NSB4);

HBV (envelope protein); HIV; RSV and others

Tumor Associated Antigens: MelanA; MAGE (A1, A3,A4); PSA; WT1; MVA (180L,

105L, 189R ...); CEA; SOX; Histones; p53; Survivin; Tarp; NY-ESO; Prame; c-Myc and others

A full up-to-date list can be found on:http://www.jpt.com

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Sub-Pool IVMarkerPeptide

Sub-Pool I Sub-Pool II Sub-Pool IIIMarkerPeptide

MarkerPeptide

MarkerPeptide

Individual Peptides

PepMix™

Antigen spanning or mixed epitope pools for in-vitro stimulation of CD4+ and CD8+ T-cell responses. Both pre-made and customized peptide pools are available.

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Selected References> “T Cell Infiltrates in the Muscles of Patients

with Dermatomyositis and Polymyositis Are Dominated by CD28null T Cells”

Fasth et al. The Journal of Immunology (2009)

> “MMP11: A Novel Target Antigen for Cancer Immunotherapy”

Peruzzi et al. Clin. Cancer Res (2009)

> “Enhancing the in Vivo Expansion of Adoptively Transferred EBV-specific CTL with Lymphodepleting CD45 Monoclonal Antibodies in NPC Patients”

Louis et al. Blood (2009)

> “Monoculture-Derived T Lymphocytes Specific for Multiple Viruses Expand and Produce Clinically Relevant Effects in Immunocompromised Individuals”

Leen at al. Nat. Med. (2006)

“I have used JPT‘s peptide pools (PepMix) for years now, with great satis-faction! First CMV derived peptides and now also EBV. So far we have published data in one publication and plan several more in the near future.”Anna Karin Lidehäll, PhD, Uppsala University, Clin. Immunol., Uppsala, Sweden

“The focus of the Clinical Immunology Group at the German Rheumatism Research Centre is to study the role of cells and molecules in the origin and progres-sion of autoimmune and other diseases. In our hands PepMixes™ were found to be effective in monitoring the immune status of various patient populations but show also promise for the development of novel immuno therapy approaches.”Andreas Thiel, PhD, German Rheumatism Research Center, Clin. Immunol., Berlin, Germany

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Options and Specialties

• Fluorogenic and chromogenic peptides • Internally quenched peptides

(Abz/nitroTyr, EDANS/DABCYL, MCA/DNP) guaranteed without flourescent impurities

• Immunogenic peptides (MAPs, palmitinylation, Pam3Cys labeling, etc.)

• Phospho-peptides and peptidomimetics (amide bond isosteres, non-natural amino acids, etc.)

• Non-commercial building blocks available • Labeling

(non-radioactive isotopes, chromophores, etc.) • Site-directed conjugations with KLH, BSA,

ovalbumin or other carriers • Cyclic peptides

(disulfide bridges, lactams, thioether-bridges, etc.) • Long peptides (> 70 amino acids)• Scales ranging from 1 mg to several grams

Please inquire for additional services not listed here

Benefits of JPT´s Custom Peptides

• Proprietary synthesis technologies warrant fastest turnaround & most competitive pricing

• Reliable and stringent QC/QA• ISO 9001:2008 and GCLP compliance• Rapid order processing • Large variety of chemistry protocols• Fully automated pooling, aliquoting and vialing• Solubility & stability testing• Personal consultation with experienced scientists • Highest purities available (> 95 %, > 97 %)• Full range of analyses including LC-MS (trap and/or

quad), MALDI-MS, HPLC, AAA, NMR, CE, as well as peptide content determination to confirm the identity and demonstrate the high quality of our peptides

• Substantial, long-standing expertise in providing custom peptides

• Highly skilled and committed scientific staff

Freeze dried peptides will be delivered with full analytical coverage.

Automated synthesis allows a largevariety of scales and chemistries.

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Custom Peptides

The exceptional quality and reliability of this service has been appreciated by customers worldwide for many years. JPT is the premier provider of custom peptides and related services.

Selected References> “Structural Basis for Ca2+-Independence and

Activation by Homodimerization of Tomato Subtilase 3”

Ottmann et al. PNAS (2009)

> “Elimination of Immunodominant Epitopes from Multispecific DNA-Based Vaccines Allows Induction of CD8 T Cells That Have a Striking Antiviral Potential“

Riedl et al. The Journal of Immunology (2009)

> “Induction of Wilms’ Tumor Protein (WT1) Specific Antitumor Immunity Using a Truncated WT1-Expressing Adenovirus Vaccine”

Osada et al. Clin. Cancer Res. (2009)

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Quality Assurance

• JPT´s entire peptide production, purification and analysis procedures are backed by astringent DIN ISO 9001:2008 certified Quality Management System.

• All quality relevant processes are well documented and regulated according to a comprehensive SOP system.

• All peptide production is performed at JPT´s headquarters in Berlin, Germany under continuous quality measures.

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“We utilize JPT’s peptides and pools for T-cell epitope mapping and immune monitoring applications such as ELISPOT and ICS, as well as CFC. The combination of synthesis economy, quick turnaround time and tailored specifications are key to our success. JPT’s scientific competence is invalu-able for all institutions planning to employ peptides in immuno assays for research or clinical setups.”Johannes Hampl, PhD, Anza Therapeutics, Inc Concord, CA, USA

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Ultra-high-throughput, small scale synthesis generating your:

> Antigen spanning peptide library

> Proteotypic peptide sets

> Random peptide libraries

> Peptide biomarker library

> Peptide substrate collection

> Peptide scans covering entire pathogenic proteomes

Applications for Micro-Scale Peptides

• Screening for proteotypic peptides • Validation and optimization of SRM assays • Systematic biomarker screening • T-cell epitope discovery • Optimization of peptide lead structures • Generation of peptide pools for immune

monitoring • Screening assays for biological activity • GPCR deorphanization

What are Micro-Scale Peptides?

Our SPOT technology is the fastest and most eco-nomic peptide source on the market. Annually more than 3 million peptides are synthesized. The result-ing singular peptides are provided as lyophilized materials in microtiter plates.

> Amounts of 50-250 nmol per peptide> Peptide length up to 20 aa> Ready-to-use peptides in 96- or 384-well plates

Benefits of Micro-Scale Peptides

• Highly parallel synthesis approach (30 000 peptides/ week)

• Pricing: from 7 $/€ / peptide • Full analysis (MALDI, LC-MS) available• Non natural and isotopically labeled amino

acids possible• Purified peptides available• Peptide pools available upon request

Your Micro-Scale Peptide Sets will be delivered freeze dried in microtiter plates.

Documentation includes QC / QA report and plate alignment.

Selected References

> “ Evaluation of Antigen Specific T-Cell Responses with a Miniaturized and Automated Method” Li Pira et al. Clin. Vaccine Immunol. (2008)

> “Peptide Arrays on Cellulose Support: SPOT Synthesis, a Time and Cost Efficient Method for Synthesis of Large Numbers of Peptides in a Parallel and Addressable Fashion”

Hilpert et al. Nature Protocols (2007)

> ”Coherent Membrane Supports for Parallel Microsynthesis and Screening of Bioactive Peptides”

Wenschuh et al. Biopolymers (2000)

> “Spot Synthesis: an Easy Technique for the Positionally Addressable, Parallel Chemical Synthesis on a Membrane Support”

Frank et al. Tetrahedron (1992)

“Our teams at the Vrije Universiteit Brussel deal with a wide variety of projects in differ-ent areas of applied biological sciences. For many of them we are in need of peptide related tools and services ranging from Micro-Scale peptide libraries and highly purified peptides to peptide arrays. In our search for an integrated and reliable provider we came across JPT. Meanwhile, we are collaborating with JPT for many years and are very satisfied with the relationship which led to several well received publications. Especially, their unique Micro-Scale Peptides helped us to enhance our knowledge on different protein-protein interactions such as Chaperone-substrate recognition.”Prof. Joost Schymkowitz & Prof. Frederic Rousseau, VIB Switch Laboratory, Vrije Universiteit Brussel, Belgium

Micro-Scale Peptides represent the most economic custom peptide source in the market.

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Your customized high content microarray could contain

> Peptide scans through antigens or whole genomes

> Huge random peptide libraries

> Tumor associated antigen scans

> Annotated phosphorylation sites

> Annotated cleavage sites

Applications for PepStar™

• Peptide biomarker discovery• Seromarker identification & validation• Mutiplexed immune monitoring in clinical trials• Elucidation of antigen and epitope spreading

during disease progression and therapeutic intervention

• Mapping of immunodominant regions in antigens

• QC / QA of therapeutic biologics • Vaccine target identification• Identification & optimization of enzyme

substrates

What are PepStar™ peptide microarrays?

Large numbers (up to 50 000) of peptides are N-termi-nally attached to glass slides by directed and chemo-selective immobilization. Patented high-throughput synthesis of peptides results in high-content peptide arrays. Yield of synthesis is sufficient to generate hundreds of identical slides. Incubation can be per-formed with proteins and patient samples. Read-out is achieved by fluorescence using validated protocols and commercial equipment.

Benefits of PepStar™

• Cost effective provision of hundreds of identical microarrays from a single synthesis batch

• Directed immobilization of purified peptides • Flexible co-immobilization of controls• Chemical synthesis and analysis warrant batch-

to-batch reproducibility • High shelf stability • High assay sensitivity• Defined posttranslational modifications

are possible• Commercial incubation and read-out equipment

can be applied • Low consumption of patient materials and

proteins • Incubation and read-out protocols available

Your PepStar™ microarrays are delivered with detailed QC / QA documentation and application protocol.

State-of-the-art printing devices support high accuracy and batch-to-batch reproducibility.

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Selected References> “Peptide Microarray – Based Identification of

Mycobacterium Tuberculosis Epitope Binding to HLADRB1 0101, DRB1*1501 and DRB1*0401”

Gaseitsiwe et al. Nature (2009)

> “Development of a Novel Peptide Microarray for Large-Scale Epitope Mapping of Food Allergens”

Lin et al. Clin. Vaccine Immunol. (2009)

> “Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease”

Lorenz et al. Methods in Molecular Biology, Epitope Mapping

Protocols (2009)

> “Pattern Recognition in Pulmonary Tuberculosis Defined by High Content Peptide Microarray Chip Analysis Representing 61 Proteins from M. tuberculosis.”

Gaseitsiwe et al. PLoS (2008)

> “Validation of Peptide Epitope Microarray Experiments and Extraction of Quality Data”

Nathmann et al. Journal of Immunological Methods (2007)

> “Prion Recognition Elements Govern Nucleation, Strain Specificity and Species Barriers”

Tessier & Lindquist Nature (2007)

“One focus of our group is to decipher the nature of immune responses by identification of biomarkers and indicators of immune protection. With the support of JPT‘s high content peptide microarray platform, we created a peptide chip which contains 22 000 individual peptides. This enabled the visualization of the B-cell „signature“ in individuals with TB-infection vs. non-infected individuals. In our hands, JPT‘s peptide microarrays turned out to be very robust tools to identify novel peptide based biomarkers in the context of novel diagnostics and vaccine target identification.”Prof. Markus Maeurer, Karolinska Institute, Solna, Sweden

Layout of our PepStar™ microarrays:For quality control three identical subarrays are printed onto each single microarray. All peptides are chemoselectively immobilized via their N-terminus and a linker.

Layout of a single subarray on a PepStar™ microarray: Spots I, II, III and IV are control proteins (e.g. antibodies or full length antigens).

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Your customized cellulose bound peptide library could display

> Peptide scans through antigens

> Random peptides

> Positional scans

> Alanine & D-amino acid scans

> Truncation libraries

Applications for PepSpots™

• Antibody epitope mapping & characterization• Characterization of protein contact sites between - Receptor-ligand - Enzyme-substrate - Protein modules in signal transduction - Recognition sequences for heat shock proteins• Systematic optimization of peptide lead structures

What are PepSpots™ ?

Peptides (up to 20 mers) are synthesized directly on a cellulose membrane. They are C-terminally attached via a flexible linker. The membrane can be used directly for incubation with antibodies or other pro-teins and binding events read-out via chemilumines-cence. Membranes will be delivered with a detailed application protocol.

Benefits of the PepSpots™ peptidemembranes

• Incubation by standard equipment• Detection using routine ELISA protocols• Rapid, economical, and flexible synthesis

of any set of peptides • Hydrophilic cellulose membranes minimize

unspecific interactions • Detection of low affinity interactions• Incubation protocols available

Above: Automated synthesis of PepSpots™ membrane.

Left: Your PepSpots™ package includes: PepSpots™ membrane, data CD and application protocol.

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Selected References> “SAP18 Promotes Kruppel-dependent

Transcriptional Repression by Enhancer-specific Histone Deacetylation”

Matyash et al. The Journal of Biological Chemistry (2009)

> “Accurate Prediction of DnaK-Peptide Binding via Homology Modelling and Experimental Data”

Van Dumme et al. PLoS Computational Biology (2009)

> “The B-Cell Epitope of the Monoclonal Anti-factor VIII Antibody ESH8 Characterized by Peptide Array Analysis”

Albert et al. Thromb. Haemost. (2008)

> “Molecular Basis of the Interaction Between the Antiapoptotic Bcl-2 Family Proteins and the Proapoptotic Protein ASPP2”

Katz et al. PNAS (2008)

> “Crystal Structure and Functional Analysis of the Protein-Disulfide Isomerase Related Protein ERp29”

Barak et al. Journal of Molecular Biology (2008)

“My group at the University Clinics in Düsseldorf works on the investigation of protein misassembly, misfolding or misprocessing in brain diseases. Our research has an ongoing need for peptide related tools that allow systematic elucidation of such protein pathologies. JPT’s PepSpots™ arrays and JPT’s ability to reproducibly assemble even challenging and modified peptides contributed to a noticeable degree to the success of our work.”Prof. Dr. med. Carsten Korth, Institute for Neuropathology, University of Düsseldorf, Germany

For a typical order we synthesize overlapping peptides of your protein of interest on cellulose membranes. Resulting PepSpots™ membranes can be incubated with your sample and binding region detected by chemiluminescence read-out.

Antigen of interest Overlapping peptides PepSpots™ membrane Result of incubation Binding region

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Applications for our profiling service

• Identification of seromarkers for cancer, infectious & autoimmune diseases and allergies

• Peptide biomarker validation • Multiplexed immune monitoring in clinical trials• Elucidation of antibody signatures in vaccination

studies• Monitoring of humoral immune responses • Identification of novel vaccine targets • Substrate identification and specificity testing

Benefits of our profiling service

• Take advantage of our proprietary peptide microarray platform

• Well established assay conditions for seroscreening, protein-protein interactions and enzyme profiling

• All sample handling and profiling in environment regulated by DIN ISO 9001:2008 and GCLP

• Flexible co-immobilization of controls• Strong bioinformatic support for array design

and data interpretation

What services are provided?Send us a short outline of your project and we will:

> Suggest the appropriate array platform to be used

> Provide bioinformatic support for peptide and microarray design

> Provide project proposal & quotation> Perform synthesis of peptides and generate

peptide arrays > Incubate peptide arrays with your protein or

patient sample and perform control experiments> Evaluate and interprete data - Assign signals to peptide sequences - Provide documentation of results> Provide comprehensive and confidential report

Epitope Discovery & Antibody Profiling Service

JPT offers a comprehensive profiling and screening service using its peptide array platforms PepSpot™ and PepStar™ with your antibody / protein preparations or patient samples. JPT will provide comprehensive profiling reports with all results and detailed data analysis.

Let us incubate our PepStar™ peptide microarrays with your antibody, protein or patient serum.

PAT A1

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Schematic representation of aPepStar™ peptide microarraydisplaying 3 identical subarrays.

Fluorescence image of a single block within subar-ray after incubation with human serum and fluo-rescently labeled secondary antibody.

Results from incubations with different patient sera. Heatmap of signal intensities for peptides scanning the sequence of a single protein (y-axis) incubated with different patient sera (x-axis).

Scatterplot of signal intensities of differentsubarrays.

Data analysis

Check of dataquality

Microarray Layout and Data Analysis

Incubation

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Applications for BioTides™

• Identification and optimization of kinase-, phosphatase-, acetyltransferase- and histone deacetylase-substrates via standard screening systems (AlphaScreen, FlashPlates, SPA-Beads, and many more)

• Mapping of protein/protein interaction sites • Peptide ELISA assays• Production of peptide microarrays • Loading of columns for affinity chromatography

What are BioTides™?BioTides™ are custom synthesized inexpensive sets of small scale biotinylated peptides. Thousands of BioTides™ are available within days.

> Amounts of 50 - 250 nmol per peptide> Peptide length up to 20 aa > Ready-to-use soluble peptides in 96- or 384-well

plates delivered freeze dried

Benefits of BioTides™

• Thousands of unpurified biotinylated peptides for screening and peptide array production

• Unmatched turnaround times (10 000 peptides per week!)

• Delivery in ready-to-use microtiter plates • Lowest price in the industry due to patented

technology (0.99 $/€ per amino acid residue)• Complete QC (LC-MS, MALDI etc.) and aliquotation

service available

Selected References> “GP4 of Porcine Reproductive and Respiratory

Syndrome Virus Contains a Neutralizing Epitope that is Susceptible to Immuno-Selection in Vitro”

Costers et al. Arch. Virol. (2010)

> “Novel Mediators of Amyloid Precursor Protein Signaling”

Swistowski et al. Journal of Neuroscience (2009)

Your BioTides™ will be delivered in 96-well plates with detailed documentation and QC / QA report on a CD-ROM.

Use of BioTides™ for binding and enzymatic assays.

BioTides™

Biotinylated peptides for your biomedical assays using streptavidin coated beads, membranes, glass slides or microtiter plates.

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Applications for our bioinformatics service

• Design of peptide content for high-density peptide microarrays and complex peptide libraries

• Generation of homology models and use of available structural information for peptide selection

• Optimization of hit and lead structures (affinity, physicochemical properties, ADME)

• Prediction and modeling of data• Scaffold design for native-like presentation

of peptides• Interpretation, management and integration

of data

Benefits of our bioinformatics service

• Long term track record on the discovery and development of peptides in drug discovery and diagnostic development

• State of the art prediction, data interpretation and data mining algorithms and software paired with chemical and biological know-how

• Expertise available as a fee-for-service or in collaborative partnerships

Service Specifications

• Detailed discussion of your project and definition of a suitable strategy based on scientific feasibility and experience

• Receive project proposal on how our bio- and cheminformatic expertise can support your project

• Obtain detailed and comprehensive service reports

Selected references:> “Translating Peptides into Small Molecules” Hummel et al. Mol. BioSyst. (2006)

> “Design and Synthesis of a New Class of Selective Integrin α5β1 Antagonists”

Stragies et al. J. of Medicinal Chemistry (2007)

JPT has a long standing and focused expertise to escort biomedical screening and peptide lead optimization programs with its comprehensive bioinformatic and cheminformatic capabilities.We offer this unique know-how and expertise as part of our high content peptide microarray and library services or in R&D collaborations focusing on peptide hit discovery and optimization.

Bioinformatics & Cheminformatics

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Use of homology model for the selection of cytosolic loop peptides for a GPCR (MSH).

X-ray loop structure (left) and model (right) stabilized by scaffold.

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RepliTope™ microarrays are delivered together with application protocol, gal-file and QC/QA report.

JPT’s printing tools for high-content microarray generation.

Applications for RepliTope™

• Efficient profiling of antibody collections directed against the same antigen

• Identification of biomarkers for infectious and autoimmune diseases, allergy and cancer

• Monitoring humoral immune responses using sera in preclinical and clinical trials

• Characterization of potential therapeutic antibodies

• Elucidation of protein-protein and enzyme -substrate interactions in general

• In-vitro study of anti-antibody / neutralizing antibody action

• Development of phosphorylation dependent antibodies

• Monitoring of side effects of protein drugs during clinical trials

What is RepliTope™?Catalog Peptide Microarrays that contain peptide scans through a large number of relevant antigens or random peptide libraries. Every peptide is present 3 times on each microarray to ensure reproducibility of results.

Benefits of RepliTope™

• Ready-to-use RepliTope™ peptide microarrays through hundreds of relevant antigens available within days

• Compared to protein arrays: high shelf stability, high batch-to-batch reproducibility, regio-specific immobilization of peptides

• Optimized surfaces and built-in quality controls • Fully compatible with standard ELISA protocols • Flexible and economical access to many

identical peptide microarrays• Reliable epitope mapping using minimal

amounts of antibody• All in one service available (incubation, data

analysis and a detailed report)

RepliTope™

RepliTopes™ are premade peptide microarrays displaying peptide scans (15 mers, overlapping by 11 aa) through annotated proteins or random peptide libraries on glass slides. Read-out is performed via fluorescence. Every peptide is present in 3 copies on each microarray to ensure reproducibility of results.

Selection of available RepliTopes™

Bacillus anthracis, BKV, Dengue virus, EBV, HIV, CMV, Influenza, tumor associated antigens, random peptide libraries...

A full up-to-date list can be found on:http://www.jpt.com

Selected References> “Pattern Recognition in Pulmonary Tuberculosis

Defined by High Content Peptide Microarray Chip Analysis Representing 61 Proteins from M. tuberculosis.”

Gaseitsiwe et al. PLoS (2008)

> “Validation of Peptide Epitope Microarray Experiments and Extraction of Quality Data”

Nahtman et al. J. Immunol. Methods (2007)

> “Prion Recognition Elements Govern Nucleation, Strain Specificity and Species Barriers”

Tessier & Lindquist Nature (2007)

> “High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology”

Schutkowski et al. Angew. Chem. Int. Ed (2004)

> “Profiling of Generic Anti-Phosphopeptide Antibodies and Kinases with Peptide Microarrays Using Radioactive and Fluorescence-Based Assays”

Panse et al. Molecular Diversity (2004)

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Volmerstraße 5 (UTZ)12489 BerlinGermany

P. O. Box 2619Acton, MA 01720USA

USA/Canada T 1-888-578-2660F 1-888-578-2666

European Head Office T +49-30 - 6392-7878F +49-30 - 6392-7888

JPT Peptide Technologieswww.jpt.com | peptide@ jpt.com

We take pride in our competent service and swift response.Please do not hesitate to contact us for further information.We also very much welcome your feedback and comments.