Immune System

• Objectives– To select genes that are embryonic lethal• Or accept bone marrow from other centres

– Generate bone marrow chimeras• Or generate conditional mutants for EUCOMM allels

– Analyse phenotypes of these mutants and• Establish the course of embryonic lethality

Members of the Network• Principal Investigator• Werner Muller, University of Manchester, Faculty of Life Science, AV Hill

Building, M13 9PT Manchester; Email: Werner.muller@manchester.ac.uk• Co-Investigators• Immunology: Richard Grencis, Kathryn Else, Mark Travis, Sheena

Cruikshank, Douglas Millar, Dr. Mat Hardman, Paul Lyons, Jim Middleton• Immune Toxicology: Ian Kimber• Neurobiology: Emmanuel Pinteaux, Stuart Allan• Cardiology: Ludwig Neyses, Elizabeth Cartwright, Delvac Oceandy• Developmental Biology: Mike Dixon, Kathryn Hentges • Human Genetics: Yanick Crow• Bioinformatics: Andy Brass, Robert Stevens• Manchester Centre for Nuclear Hormone Research in Disease: David Ray

Example 1

Analysis of a B cell phenotype for an embryonic lethal mutation (polymerase beta)

Example 2

Analysis of a complex phenotype for an embryonic lethal mutation using conditional

gene targeting (gp130)

neurological, cardiac, hematopoietic, immunological, hepatic and pulmonary

neurological, cardiac, hematopoietic, immunological, hepatic and pulmonary

Immune System

• Objectives– To select genes that are embryonic lethal• Or accept bone marrow from other centres

– Generate bone marrow chimeras• Or generate conditional mutants for EUCOMM allels

– Analyse phenotypes of these mutants and• Establish the course of embryonic lethality