iLife Catalog

e mail : [email protected] www.ilifediscoveries.com

Building Stronger R & D Relations inBio & Pharma Sciences

through complete Technology Solutions

As a unique discovery and contract research organization, iLife Discoveries provides

molecular biology and preclinical drug discovery services to pharmaceutical, biotechnology,

academia and Public & Private Institutions on an outsourced basis. With the advent of

recent scientific and technological advances, iLife Discoveries has broadened its services

& solutions portfolio in order to accelerate researchers in all phases of the early-stage

discovery process and support them in streamlining their custom discovery and

development programs. iLife also specializes in complex experimental designs, protocol

development and execution. iLife has also established itself as a leader in expertise

development through high end technology trainings in pharma and life sciences sector.

Global PresenceiLife is Headquartered in the Silicon Valley, USA, with its Global Discovery Center

establised in the Delhi NCR region of India. The global visibility and India advantage thus

converge to create attractive features of cost benefit, technology expertise, adherence to

national and international Intellectual Property rules and rapid access to qualified human

resources. With a wealth of in-house scientific expertise, world class R&D facility, and

the most advanced technologies, iLife is strategically possitioned to deliver Quality, Speed,

Competitive Costs, Flexibility, Custom Solutions and is the Contract Research Organization

of choice for the Life Sciences & Pharma Industry.

The TeamThe iLife team is committed to excellence. The highly skilled scientific staff is comprised

of individuals having a breadth of expertise including preclinical, academic research, and

CRO research experience. Our research group and technical specialists have a combined

40+ years of global experience working in multiple areas of research in infectious diseases,

oncology, molecular diagnostics, bioagriculture, metabolic disorders to name a few. The

management team and staff offer their expertise in working with you to assure the success

of the design and implementation of your project, within the timelines. iLife’s commitment

to quality is second to none, piloted by an experienced leader in the life sciences domain.

Our CapabilitiesLife sciences research and discovery process is highly complex, lengthy, and expensive,

yet essential for delivering safe and effective systems for improvising human healthcare.

Even though the process is reasonably well-defined, each program is fundamentally

different and shaped by many unique factors. To help our customers navigate this process,

iLife offers a comprehensive range of contract research solutions and services that span

the entire discovery continuum and can be tailored to specific research conditions. Our

portfolio covers a broad array of basic research in the molecular biology workflow and

preclinical research. We undertake research projects in cell biology, genomics, proteomics,

bioinformatics, molecular diagnostics, High throughput screening and drug discovery

studies which are designed to accelerate the pace and increase the productivity of your

discovery and development programs.

Our offerings are continually expanding based on the changing needs of our customers

and our team of experts understands and ultimately assists our customers by providing

services to span all stages of research discovery while complying with international

scientific regulations.

Complete and Customized

Biological Services and Solutions

Consulting – Developing Solutions TogetherA long-standing experience in molecular and cell biology forms the basis of iLife’s exceptional

consulting services. This service addresses the needs of research and business partners

operating in the fields of biotech, pharma and diagnostics. During the development and

standardization of a new application or protocols or instrument platform, our customers have

to make important decisions. With our flexible consulting service, we effectively support our

partners in achieving their goals. Our consultations are project specific process with a set of

recommendations for our customers. These help to define practical work flows which then

can be outsourced to iLife Discoveries. Let us sit down, join forces and develop solutions

together.

The FacilityDoing what’s best for our clients means furnishing a full range of equipment backed by a

smart, experienced team to get them through the difficult and challenging research and

discovery process. The Global Discovery Center is an ultra modern, 30,000 sq. ft., state-of-

the-art facility and houses India’s largest CLASS-10000 clean room laboratory cluster

exclusively dedicated to biological research and development for our customers. A US FDA

209E Code compliant facility is equipped with latest high throughput part 21 CFR compliant

technologies including Agilent Microarrays, Automated Liquid Handling workstations,

sequencers, HPLC, microfluidics based technologies for analysis, QPCR, OCTET RED for

biomolecular interactions, Flow Cytometers, and others.

CollaborationHaving several active and ongoing collaborative research programs, iLife continues to seek

academic, government and commercial research partnerships in HTS, diagnostics, food testing,

forensics, agriculture and drug discovery. We are interested in collaborating to pursue

government funding for joint efforts including grant applications and competitive contracts.

For gaining its own intellectual property, iLife funds several in-house drug discovery program

for the purpose of developing commercially relevant intellectual property. Contact our team

for more information on establishing a collaboration.

The iLife Advantage• Partnership in R&D from concept to operational strategies- end to end solutions

• Accommodating to ever demanding challanges of faster deliverables

• Personalized attention at every stage of the work dedicated teams for individual project

• Fostering frequent, open communication during all phases of a study

• Reproducible results and innovative data analysis to help accelerate decision making

• Managing the integrity, compliance, timeliness, format, and confidentiality of your data

• Time tested logistics and client support worldwide- customized modules for sample storage

and shipment through our India - US operations

• Digital documentation of individual projects on proprietary iLife LIMS platform

• Strict adherence to international, business practices, regulatory compliances, transparency

and accountability

India’s Largest US FDA 209E code compliant class 10000 Clean

Room Laboratory Cluster dedicated exclusively for

R&D IN PHARMA & LIFE SCIENCES

India’s Largest US FDA 209E code compliant class 10000 Clean

Room Laboratory Cluster dedicated exclusively for

R&D IN PHARMA & LIFE SCIENCES

World class, ultra modern laboratoryexclusively dedicated to R & D

Customized SolutionsEvery research lab has its own requirement. We have the tailor-made solution.

With our customized solutions, iLife aims to offer the highest standards of services through custom- made

solutions. We actively manage our customer’s projects while constantly seeking value-added innovations and

opportunities. Our capabilities are based on scientific expertise and experience, state-of-the-art instruments

and technologies, and the ability to discover and develop solutions. Each customer has a dedicated scientific

manager who focuses on individual objectives and needs. For all our solutions, our focus remains on

accomplishing your research goals while maintaining confidentiality and business ethics.

Training and Expertise DevelopmentTo be successful, it is essential for organizations to put their research team through periodic training programs,

which can enhance their knowledge and competitiveness in several critical areas. At iLife Discoveries, Training

and Expertise Development remains an integral part for scientific human recourse development. iLife delivers

value-added customized technical, management and soft skills training solutions for the global pharma and

biotech sector. We design and deliver practical training solutions for developing specific expertise to raise the

competence of our client’s employees to best–in–industry. Our emphasis is on the practical application of

knowledge and technical expertise essential for effective working in the pharma, biotech and associated life

sciences industries. We achieve this through balanced and highly stimulating learning modules with tremendous

planning, practical applications and continuous up-gradation of our own technical expertise and resources.

If you would like to have a discussion about a customized training program or develop a training strategy for

your scientific, sales or management group, please contact the Training Co-ordinator at iLife.

Corporate Floor at iLife Discoveries

“Collaboration with external partners has today

become an essential part of every research group in

Academia or Industry working in Life Sciences

domain. At iLife Discoveries, we are proud of our

ability to establish open and collaborative

partnerships to share ideas, expertise and

capabilities to make both parties successful.”

Anand Gupta

Founder and CEO

“Seamless and real time access to multitudes of

rapidly evolving technologies and discovery

platforms have essentially become the fundamental

“mantra” of success to global academic and

corporate research involved in therapeutics and

diagnostics. With a very focused approach in the

technology intensive pre-clinical “in-vitro” biology

space, iLife Discoveries has carved its own footprint

in providing simpler solutions to complex research

problems to its expanding client list. Our rapid access

to technology combined with our ability to fast-track

their translation to innovative research problems

make us unique in the early discovery support

sector.”

Dr.

Anil Mavila

President and Technology Head

Customized Support

Because we’re as passionate about your success as you are.

Your support needs are diverse as your research and our services go far beyond traditional

notions of customer support. We will be glad to assist you with particular issues, of course –

quickly and efficiently in every step of the way. From our friendly telephone representatives to

our unmatched team of experienced technical experts and Business Coordinators, we promise

to deliver an excellent level of support you deserve from a world class organization.

If you need help choosing the right services, our experts are available to discuss your needs

and provide recommendations. Email us your contact information and area of research and

one of our experts will contact you.

If you need immediate assistance,

Please call us at +1- 408 - 907- 6645 or +91- 124 – 4779800.

For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800

(US, Canada & Europe) Call +1 408 - 907 6645

Animal Research• Identification of economic trait loci (ETL) such as

growth, meat yield etc.

• QTL mapping and marker assisted selection of the

population

• cDNA microarrays to characterize gene expression

patterns of experimental animals

• Real time PCR based assays for animal genotyping,

gene expression profiling, copy number variation

• Identification of genetic pathways important for

animal production and health.

• Identification of SNP related to disease traits

• Genotyping of animals populations based on

molecular markers

• cDNA, EST and SSH library construction

• Construction of genomic libraries

• Genome sequencing

• Transcriptome analysis using Next Gen Sequencing

• SureSelect target enrichment for Next Gen

Sequencing

• Annotation of livestock genome using human and

mouse genome

• Identifying functional element in animal genome

using information of other genome

• DNA modifications in diseased and cloned animals

• Methylation microarray

• CGH microarray

• ChIP-on-chip array

• Recombinant protein production of animal origin

• Deciphering animal development through

proteomics

• High throughput protein profiling of all animal

samples(Antibody microarray, 2-D gel

electrophoresis, off-gel fractionation)

• Identification of species specific peptides/proteins

by MALDI-TOF

• Study of Biomolecular interaction of animal proteins

(enzymes, hormones, transcription factor etc.) using

OCTET technology

• Allele Mining

• Microsatellite genotyping of livestock population

• Study of Tumor Metastasis

• Meat Quality testing using genomics and proteomics

• DNA Barcoding

• Establishment of primary cell lines

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Agrobiotechnology• Identification of desirable traits in plants

• Identification of QTL of economic traits in plants

• Genetic and physical mapping of QTL

• Construction of large insert genomic libraries

• SureSelect target enrichment for Next Gen Sequencing

• Genome sequencing strategies using Next Gen Sequencing

• Transcriptome analysis (ESTs, Microarrays, Next Gen sequencing)

• Gene expression in response to environmental stimuli (Stress – biotic & abiotic), treatment, development stage

• Gene expression profiling by microarray

• Gene expression by Real time PCR

• Epigenomics - DNA and protein modifications


• CGH microarray


• DNA sequence analysis and data handling

• Finding genes related to stress conditions

• Finding regulatory features in gene structure by comparative genome analysis

• Regulation of gene expression

• miRNA profiling by microarray


• Plant Pathogen (Fungal) genomics and proteomics

• Marker Screening and Discovery

• Integrative description of plant function (Productivity, Quality, Sustainability)

• GMO Testing by molecular methods

• Metabolite Analysis by HPLC

• Protein/peptide identification by off gel fractionation followed by MALDI - TOF

• Seed quality and genotype testing by molecular methods

• Testing the authenticity of Basmati rice samples by molecular methods

• Rapid wheat varietal identification

• Comprehensive identification of proteins, their isoforms, and their prevalence in each tissue

• Study of Biomolecular interaction of plant proteins (enzymes, hormones, transcription factor etc.) using OCTET technology

• Real time PCR based assays for plant genotyping, gene expression profiling, copy number variation

• Microsatellite genotyping of plant population

• Allele Mining


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• Species level identification using molecular techniques

• Genus level identification using molecular techniques

• Comparative analysis using molecular fingerprinting techniques

• Pathogen detection using molecular techniques

• Food Testing of microbial contamination using Real Time PCR

• Establishing evolutionary relationships by sequence analysis such as 16S

• Microbial diversity studies

• Detection of bacteria and other organisms poluting air, water, soil, and food

• Microbial identification of environmental samples

• Phylogenetic analysis

• Comparison phylogenomics of pathogenic bacteria by microarray analysis

• Toxicity pathway focused gene expression profiling

Molecular Diagnostics• Real Time PCR based detection of HIV, HPV, H1N1, Malaria, Hepatitis, TB

• Cancer diagnostic through mutation analysis

• Cancer diagnostic through gene expression profiling

• Bacterial and viral load detection by Real Time PCR

• Pathogen detection by molecular methods

• Real Time PCR/ PCR based detection of organisms for which microscopy gives false positive results e.g. T.vaginalis, N.gonorrhoeae.

• Molecular detection of intracellular pathogens such as M.genitalium.

• Identification of species such as Chlamydia sp.,Neisseria sp. where conventional method is less sensitive

• Molecular identification of species in which Subtyping is mandatory e.g. HSV, HPV, HCV

Microbiology and Pathobiology

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Oncology

• Gene expression in response to disease stage, disease

progression (Benign & Malignant)

• Comparative Gene expression profiling in cancerous

and normal tissue by Microarray & Real Time PCR

• Identification of regulation of gene expression in

different cancers

• miRNA profiling by microarray


• Biomarker Discovery, Identification , Characterization

and Validation for different cancers

• Identification of Epigenomics modification associated

with different cancers

• CGH microarray



• Transcriptome analysis in cancer cells (Next Gen

Sequencing)

• Proteomics analysis in cancer cells

• Protein Profiling by 2D Gel Electrophoresis

• High throughput Protein Profiling by Antibody

microarray

• Pathway focused Protein Profiling by Antibody

microarray

• Reverse-Phase Protein Microarray

• Protein Identification by Mass Spectrometry &

N-terminal Sequencing

• Identifying tumor associated antigens

• Profiling of disease state by antibody microarray

• Time-course studies to identify different stages of

cancers

• Identification altered genes or biochemical pathways

associated with particular cancer

• To identify new molecular classes of cancer (class

discovery)

• To predict diagnosis and classification of unknown

cancer samples (class prediction)

• Study of biomolecular interaction of cancerous protein

using Biolayer Interoferometry

Food and Nutrition Science• Profiling and characterization of dietary and other proteins

• Biomarker discovery for the nutritional status

• Proteome analysis in solving major nutrition-associated problems in

humans and animals, such as obesity, diabetes, cardiovascular

disease, cancer, aging, and intrauterine fetal retardation.

• Microbial contamination testing of food using Real Time PCR

• HPLC based food analysis

• Detection, identification and characterization of food borne-pathogens

• GMO testing

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Drug Discovery• SAR studies and initial pharcodynamics analysis

• High throughput screening for desired pathways/disease

indication/bioactivity

• Basic ADME-TOX

• Co-Culture platforms for microbial pathogenesis tests.

• Design and development of Assay systems for sub-genomic

RNA interference (antiviral drug discovery)

• FRET systems for biomolecular interactions.

• Expression, characterization and protein modification studies

Infectious Diseases• Diagnosis and monitoring of infectious diseases by molecular

methods

• Detection of pathogens

• Sequencing of microbial pathogen genome

• Cloning of disease susceptibility genes

• High throughput screening of disease for pathogens by Real

Time PCR

• Discovery, qualification and validation of Protein Biomarkers

in infectious samples

• Real Time PCR/PCR based detection of un-culturable or

fastidious microorganisms

• Molecular diagnostic of infectious diseases such as HIV, HCV,

TB, HPV, HINI, CMV etc.

Clinical Proteomics• High throughput Protein Profiling of clinical samples by Antibody

microarray

• Pathway focused Protein Profiling of clinical samples by

Antibody microarray

• Reverse-Phase protein Microarray for validation of antibody

microarray results

• Discovery, qualification and verification of Protein Biomarkers

in infectious samples

• Identification of protein related to disease by 2-D gel

electrophoresis and MALDI - TOF

• GPCR and kinase profiling

• Antibody arrays

• Gene and Transfection services

• Biomolecular interaction studies and small molecule screening

• in silico drug designing

• Customized discovery systems using mammalian yeast and

bacterial systems with multiple Promoter-Reporter

combinations




COSMODEx: Cosmeceutical Discovery using

Microarray based Expression Profiling SystemsSpecific Microarray based design and development of Animal free research systems for multifaceted testing of cosmetological products

can be accomplished under this program. Briefly, we develop and utilize novel cell lines representing epithelial keratinocytes, ectocervical

vaginal cells, buccal cavity cells, melanocytes and their corresponding tumor derived cell populations to study metabolism and kinetics of

small molecules and candidates for cosmeceutical applications at their molecular level. Using a proprietary technology, stable cell lines

are developed and their wild type (control) profile is characterized using advanced genomic tools. Panels thus created will be specific for

hypersensitivity reactions, toxicity responding genes, cellular apoptosis genes, aging and senescence pathways related genes and

metabolite converting enzyme panels, cytochrome p450 family members etc. These panels are used as reference standards for customized

research programs for identifying Cosmeceutical molecules with regards to their activity and toxicity. Additionally, these customized

chips will enable the researcher to resolve the time dependent responses of Cosmeceutical and therapeutic molecules.

Application of t-TRAP Technology for Determining Food SafetyA comprehensive and innovative method for identifying time dependent and detrimental microbial pathogens or cross contamination of

food, meat and marine products.

It has been well established that emerging economic threats associated with the safety of food and edible materials are indeed a concern

to nations that depend largely on exports from the rest of the world. Added to the complexities is the increasing threats to global food

security by engineered or man made threats such as large scale sabotage food or edible materials.

Major food pathogens like multiple species of Clostridium, Listeria, Bacillus, Yersinia, Vibrio, Botulism, Brainerd Diarrhea, Cholera,

Shigellosis etc. Specific signature sequence will be used for making specific and customized chip for targeted food products such as

Kosher meat, marine food products etc.

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CyTOSENSE : Cell repositories Sensitized to Microbial and Mycoplasma contamination

PMCI Collection : Population specific mammalian cell line repository for drug discovery containing primary & transformed cell lines

developed through rational in-sourcing & Genetic Engineering for multipanel reporter expression systems


PRIME-C : Pharmaceutically relevant Repository of Intelligent

Mammalian Cells




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Every research lab has its own requirement. We

have the tailor-made solution.



Contents

DNA Extraction & Purification 20

RNA Extraction & Purification 20

Plasmid DNA Purification 20

Agilent 2100 Bioanalyzer- Technical Note 21

DNA Analysis 21

DNA Application 21

Restriction digest analysis 22

PCR product analysis 22

Gene expression analysis 22

Food analysis 22

GMO detection 22

Oncology 22

Diagnostic research 22

Forensic testing 22

RNA Analysis 22

RNA Application 22

Analysis of total RNA 23

Analysis of mRNA 23

Analysis of Cy5-labeled samples 23

Analysis of T7-RNA transcripts 23

Analysis of Small RNA 23

Restriction Fragment length Polymorphism

(RFLP) Analysis 24

DNA Fingerprinting 25

Customized PCR Assays 26

Primer Design, Synthesis & Validation 26

PCR Analysis 26

Cloning 27

Cloning of DNA for sequencing 27

Cloning of DNA for expression of protein 28

Cloning and Sequencing of Up-stream/

Down-stream Regions from Known Sequence 28

Cloning of STRs/ Microsatellite Markers 29

Full-length cDNA Cloning Services 29

Library Construction 30

Genomic library construction 30

cDNA library construction 31

Site Directed Mutagenesis 32

Gene Sequencing 33

Ready to Run sequencing 33

Single Pass 33

Fragment Analysis 34

Microsatellite Analysis 34

SNP Detection 34

Gene Walking 34

GENOMICS

17


Service Inform

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DNA Microarrays 35

Transcriptome Analysis/ Gene

Expression Analysis 36

microRNA profiling 37

Location Analysis (Discover DNA Methylation

patterns) 38

Chip-on-chip Analysis 39

Copy Number Analysis (Comparative

Genomic Hybridization (CGH)) 40

Copy Number Variation (CNV) 41

Custom microarray development 42

Real Time PCR Assays 43

Absolute Quantification by qPCR 44

Relative/Comparative Quantitation 44

Multiplex Real Time Assay 45

Applications 45

Validation of Microarray data 45

Molecular Diagnostics 45

Infectious diseases 45

Metabolic Disorders 46

Child and Women health 46

Primer Efficiency Determination 46

DNA/RNA copy number assessment 46

Environmental testing using labeled probe 47

Microbial Diagnostics 47

Food and Agricultural 47

Screening of GMOs 47

SNP Analysis Using Real Time PCR 47

Splice Variant Detection Using Real time PCR 47

Cancer Diagnostics using Real Time PCR 48

Real Time PCR in Forensic Science 48

Next Gen Sequencing 49

Whole Genome Sequencing 49

Whole Transcriptome Analysis 49

ChIP sequencing 50

Micro RNA Analysis 50

SureSelect Target Enrichment Systems 51

Gene Synthesis 52

Bacterial Identification 53



Protein Extraction & Purification 56

Protein Analysis 56

Protein Application 56

Protein Expression 56

Protein Purification 56

Antibody Analysis 56

Food Analysis 56

Protein - Others 56

Protein Expression & Purification 57

Customized protein expression for

E. coli, yeast and mammalian cells 57

Epigenetic expression of proteins under inducible

promoters 58

Small Scale protein purification for proof of

concept studies 58

Customized protein purification platforms for

crystallography studies 59

Protein Profiling 60

Two-Dimensional Gel Electrophoresis,

followed by MALDI-TOF/TOF 60

Offgel Fractionation 60

Antibody Microarrays 61

Pathway focussed Protein Profiling 63

Immunological assays 64

ELISA Assay 64

ELISPOT Assay 64

Biomolecular Interactions Assays 65

Monitoring Antibody Concentration 65

Small Molecule Detection 65

Kinetic Profiling of Immunotherapeutics 65

Concentration Measurements based on

Binding Rates 65

Protein Quantitation in Bioprocessing 66

Kinetic Characterization Data 66

Detection of anti-drug Antibodies(ADA) 67

Screen for Drug Inhibitors 67

Antibody Production 68

Monoclonal antibodies 68

Polyclonal antibodies 68

Peptide Synthesis 69

Custom peptide synthesis 69

Metabolite analysis 69

HPLC & LC-MS Services 69

FOOD & AGRICULTURE 69

DRUG & PHARMA 69

WATER Analysis 70

MALDI ToF/ToF Services 70

Peptide Mass Fingerprint (PMF) 70

MS/MS peptide fragmentation 70

Mass Analysis- by MALDI ToF for

proteins/peptides 70

De novo sequencing of proteins/ peptides 70

Customized Cell Biology Services 71

Adenovirus Services 71

Generation of Stably Expressing Cell Lines 71

Novel Cell Lines 71

PROTEOMICS & CELL BIOLOGY

Contents

BIOINFORMATICS

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Introduction and Applications 74

Genomics 74

Comparative genomics 74

Structural genomics or structural bioinformatics 74

Functional genomics 74

Proteomics 74

Pharmacogenomics 74

Pharmacogenetics 74

Cheminformatics 74

Biomedical informatics / Medical informatics 74

Computational chemistry 74

In silico drug designing 75

Agroinformatics / Agricultural informatics 75

Systems biology 75

Microarray Data Analysis 76

Single & Two Color Gene Expression Analysis 77

Exon Expression & Exon Splicing Data Analysis 78

Copy Number Analysis 79

Association Data Analysis 79

miRNA Analysis 80

Custom Chip Design and Analysis 80

RT-PCR Data Analysis 81

Comparative Genomics 82

Biological Systems Modeling 82

Sequence Analysis 82

Evolutionary Studies 82

Gene Annotation 82

Primer Designing 83

Prediction of Protein Structure 84

Protein-Protein Interaction Studies 84

Peptide Library Synthesis 84

Antibody Microarray data analysis 85

Shipment Guidelines 86

Terms & Conditions 87

Ordering Information 87

High-end Technology Training 88



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GenomicsKnow Your Genes...



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Nucleic Acid Purification

Extraction of DNA with high purity is a necessity for many down-

stream applications. DNA extraction & purification services for small

and large-scale projects is available. Intact, high-quality genomic

DNA (gDNA) is routinely extracted from various sources including

tissue (fresh and preserved), blood, saliva, other body fluids, cell

cultures, plants,and forensics samples.

DNA Extraction & Purification

RNA Extraction & PurificationExtraction of RNA with high purity & integrity is a necessity for

much downstream application. Due to the omnipresence of RNases,

and the instability of RNA, integrity checks and sample quantitation

are essential steps before any RNA dependent application. RNA

extraction & purification services for small and large-scale projects

are available. Industry standard of RNA is routinely extracted from

various sources including tissue (fresh and preserved), blood, saliva,

other body fluids, cell cultures, plants, and forensics samples.

Plasmid DNA Purification

Extraction of Plasmid DNA with high purity is a necessity for many

downstream applications. Plasmid DNA extraction & Purification

services for small and large-scale projects is available. Intact, high

quality Plasmid DNA is routinely extracted for applications like Gene

Therapy & multiplex PCR, real-time quantitative PCR, DNA cloning,

DNA southern blot hybridization, DNA transfection, DNA

sequencing, SNPs analysis etc.

Ordering Information

For all prices contact : [email protected]

Service No. Description

GNE001 DNA Purification from Tissue

GNE002 DNA Purification from Blood

GNE003 DNA Purification from Cells

GNE004 RNA Purification from Tissue

GNE005 RNA Purification from Blood

GNE006 RNA Purification from Cells

GNE007 Plasmid DNA Purification

GNE008 Customized Nucleic Acid Purification

DNA Analysis 21

Plasmid Analysis 21

RNA Analysis 22

RFLP 24

AFLP 24

DNA Fingerprinting 25


Cloning 27

Library Construction 30

Site Directed Mutagenesis 32

Gene Sequencing 33

DNA Microarrays 35



Other Related Services

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Quantification of Biomolecules such as DNA, RNA and protein is an essential, integral and fundamental step for any life science research.

It is based on Lab-on-a-Chip technology which enables downscaling and integration of several experimental steps into one process, in

combination with software which automates data analysis and data archiving and generates a complete QC report with quantitation

estimate, virtual gel image and electropherogram.

Nucleic Acid Analysis on Agilent 2100 Bioanalyzer

The Lab-on-a-Chip Approach

The Chip technology uses microfluidics whose behavior, precise control and manipulation of fluids that are geometrically constrained to a

small, typically sub-millimeter, scale allows quantification of biomolecules with a minimum volume of 1ul. The sample moves through the

microchannels from the sample well and it is injected to the separation channel. The sample components are electrophoretically separated

and are detected by their fluorescence and translated into gel like images (bands) and electropherograms (peaks).

Agilent 2100 Bioanalyzer – Technical Note

This technology utilizes a network of channels and wells that are etched onto glass or polymer chips to build mini-labs. Pressure or

electrokinetic forces move pico liter volumes in finely controlled manner through the channels. Lab-on-a-Chip enables sample handling,

mixing, dilution, electrophoresis and chromatographic separation, staining and detection on single integrated systems. The 16-pin electrode

enables the detection of samples which connected to HV sources.

Applications for a Lab-on-a-Chip Technology

DNA Analysis

Agilent 2100 Bioanalyzer is used which is a microfluidics based platform for sizing, quantification

and quality control of DNA. Different kits allows to automatically size and quantitate PCR

fragments and restriction digests accurately and reproducibly

DNA Application

The chip technology can perform variety of applications in the life science research such as

sizing and quantitation of DNA fragments like PCR product purity, Multiplex PCR Applications,

GMO Testing, Pathogen Detection, Genotyping Applications (Duplication/Deletion, Allele

frequency), Cancer diagnostics etc...:



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RNA AnalysisAgilent 2100 Bioanalyzer is used which is a microfluidics based

platform for Industry standard of RNA quality control. RNA Integrity

Number (RIN), a quantitation estimate, and ribosomal ratios of the

total RNA sample will be provided. For mRNA samples the

percentage of ribosomal impurities is calculated.

RNA Application

This technology is the industry standard for RNA sample QC and

the Bioanalyzer Expert software generates the RNA Integrity

Number (RIN), a quantitation estimate, and will calculate ribosomal

ratios of the total RNA sample. RIN provides a numerical

assessment of the integrity of RNA—independent of concentration,

instrument, or analyst—giving the scientist a high degree of

confidence in the repeatability of the experiment. It can be used for

the analysis of total RNA, small RNA, messenger RNA in nanogram

and picogram concentration range.

Restriction digest analysis

Sizing range exemplified by the separation of Adenovirus 2/Dra I

Detection of single base mutations

PCR product analysis

Separation of different mixtures of PCR products

Determination of PCR product impurity

Multiplex PCR analysis of bacteria in chicken

Gene expression analysis

mRNA expression study by comparative multiplex PCR

Standardized end-point RT-PCR

Co-amplification and response curves

Competitive PCR

Food analysis

Development of meat specific assays

Sea food authencity testing

Fish species identification by RFLP

Strawberry and Raspberry food differentiation.

GMO detection

Basmati Rice authencity testing

Development of a multiplex assay for soya

DNA stability during food processing

GMO detection by nested multiplex PCR

Oncology

Tumor cell detection from carcinoma patient blood

SNP analysis in cancer related P16 gene

K-ras gene SNP detection

METH-2 downregulation in lung carcinomas

Label-free analysis of microsatellite instability in carcinoma

Diagnostic research

Genotyping of H. pylori

Duplications and deletions in genomic DNA

Forensic testing

Optimization of PCR on mtDNA

Note: We offer Customized solutions for nucleic acid

applications. Please contact technical support for further

assistance.





GDA001 Sizing range exemplified by the

separation of Adenovirus 2/Dra I

GDA002 Detection of single base mutations

GDA003 Separation of different mixtures of PCR products

GDA004 Determination of PCR product impurity

GDA005 Multiplex PCR analysis of bacteria in chicken

GDA006 mRNA expression study by

comparative multiplex PCR

GDA007 Standardized end-point RT-PCR

GDA008 Co-amplification and response curves

GDA009 Competitive PCR

GDA010 Development of meat specific assays

GDA011 Sea food authencity testing

GDA012 Fish species identification by RFLP

GDA013 Strawberry and Raspberry food differentiation.

GDA014 Basmati Rice authencity testing

GDA015 Development of a multiplex assay for soya

GDA016 DNA stability during food processing

GDA017 GMO detection by nested multiplex PCR

GDA018 Tumor cell detection from carcinoma patient blood

GDA019 SNP analysis in cancer related P16 gene

GDA020 K-ras gene SNP detection

GDA021 METH-2 downregulation in lung carcinomas

GDA022 Label-free analysis of microsatellite

instability in carcinoma

GDA023 Genotyping of H. pylori

GDA024 Duplications and deletions in genomic DNA

GDA025 Optimization of PCR on mtDNA

GDA026 Customized DNA Analysis



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Analysis of total RNA

RNA integrity and documentation of RIN number

Reproducibility of quantitation

Detection of low levels of RNA

RNA integrity for picogram level detection

Genomic DNA contamination

Analysis of mRNA

RNA integrity

Ribosomal RNA contamination in mRNA samples

Analysis of Cy5-labeled samples

Analysis of cRNA with and without dye in gel matrix

Optimization of labeling reactions

cRNA fragmentation

Analysis of T7-RNA transcripts

Size estimation

Analysis of Small RNA

Note: We offer Customized solutions for nucleic acid applications.

Please contact technical support for further assistance.





GRA001 RNA integrity and Documentation of RIN number

GRA002 Reproducibility of quantitation

GRA003 Detection of low levels of RNA

GRA004 RNA integrity for pico gram level detection

GRA005 Genomic DNA contamination

GRA006 RNA integrity

GRA007 Ribosomal RNA contamination in mRNA samples

GRA008 Analysis of cRNA with and without dye in gel matrix

GRA009 Optimization of labeling reactions

GRA010 cRNA fragmentation

GRA011 Size estimation

GRA012 Analysis of small RNA

GRA012 Customized RNA Analysis

Protein AnalysisA specific chip design allows the analysis of upto 10 protein samples

in the 5-250KDa size range with the use of different kits. A variety

of different samples for can be used such as column fractions,

purified proteins, antibodies and cell lysate.

Figure : Direct Comparison of samples run on SDS PAGE with silver staining

and on Agilent Bioanalyzer. Concentrations are given per lane.

Cell Fluorescence Analysis

The Agilent Bioanalyzer is used for cell assays for analysis of cell

fluorescence parameters for flow cytometric studies. It works on

the principle of Pressure driven flow which is used to move cells in

a controlled manner through the microchannels which are

hydrodynamically focused to a portion of a channel by a side stream

of buffer and cells pass the fluorescence detector in a single file

and each event is monitored in a histogram or a dot plot.

It can be used for many applications such as Apoptosis,

Transfection efficiency monitoring, protein expression monitoring

and gene silencing.

Figure: Flow cytometry on a chip. Two Color detection and a dot plot

view for easy data evaluation.

Figure : Electropherogram of RNA analysis



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Restriction Fragment Length Polymorphism (RFLP) Analysis

RFLP refers to a difference between two or more samples of

homologous DNA molecules arising from differing locations of

restriction sites.

RFLP Analysis services are offered for the following

applications:• Genome mapping

• Cloning

• Localization of genes for genetic disorders

• Determination of risk for disease

• Genetic fingerprinting

• Characterization of genetic diversity

• Breeding patterns in animal populations

And many more……

DNA 7500 and DNA 12000 kits

Agilent DNA 7500 and DNA 12000 kits are designed for the sizing

and quantitation of double-stranded DNA fragments from 100 to

7500 base pairs (DNA 7500) and 100 to 12000 bp (DNA 12000)

respectively. These kits can be used to analyze, for example, PCR

and RT-PCR products as well as restriction digests.

Fragment Analysis for RFLPAgilent 2100 Bioanalyzer is used which is a microfluidics based

platform for sizing, quantification and quality control of DNA.

Different kits allows to automatically size and quantitate restriction

digests accurately and reproducibly.

DNA 1000 Kit

It can be used for sizing and quantitation of double-stranded DNA

fragments from 25 to 1000 base pairs. It can be used for the following

applications:

• RFLP analysis

• Heteroduplex analysis using mismatch cleavage

enzymes Ordering Information


GRA001 RFLP with agarose gel analysis

GRA002 RFLP with Bioanalyzer analysis

GRA003 RFLP with Sequencing analysis



Figure : Electropherogram of RFLP on Agilent 2100 Bioanalyzer

Figure : RFLP representation on Agilent 2100 Bioanalyzer



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DNA Fingerprinting

Fingerprints are able to determine whether two DNA samples are

from the same person, related people, or non-related people. Small

numbers of sequences of DNA that are known to vary among

individuals are analyzed to get a certain probability of a match.

Applications:

• Paternity and maternity testing

• Criminal Identification and Forensics

• Personal Identification

Fragment Analysis

Agilent 2100 Bioanalyzer is used which is a microfluidics based

platform for sizing, quantification and quality control of DNA.

Different kits allows to automatically size and quantitate PCR

fragments and restriction digests accurately and reproducibly.

DNA 1000 Kit

It can be used for the following applications:

• Analysis of PCR and RT-PCR products

• RFLP analyses

DNA 7500 and DNA 12000 kits

Agilent DNA 7500 and DNA 12000 kits are designed for the sizing

and quantitation of double-stranded DNA fragments from 100 to

7500 base pairs (DNA 7500) and 100 to 12000 bp (DNA 12000)

respectively. These kits can be used to analyze, PCR and RT-

PCR products as well as restriction digests.



GDF001 DNA fingerprinting, fragment sizing by

agarose gel and fragment analysis by sequencing

GDF002 DNA fingerprinting, fragment sizing with

bioanalyzer and fragment analysis by sequencing





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Custom PCR Assays

Industry standard customized PCR Assays services are provided

which includes primer designing, synthesis, standardization &

optimization of PCR. Detection & analysis of the PCR product is

done on High-end Lab-on- Chip technology.

Custom PCR Assays services are offered by iLIFE for the

following applications:

• Amplification & Quantification of DNA

• Selective DNA isolation for genetic Fingerprinting, generating

hybridization probes for Southern or northern hyb. and DNA

Cloning, DNA sequencing etc.

• Diagnosis of diseases

• Species identification

• Multiplex-PCR for analysis of microsatellites and SNPs etc.

And many more……

PCR Analysis

Microfluidics technology of Agilent 2100 Bioanalyzer is used for

sizing, quantification and quality control of DNA.

Different kits allows to automatically size and quantitate restriction

digests accurately and reproducibly:



GPR001 Primer Designing, Synthesis and Validation

GCP001 Custom PCR Assay


Sample Submission Requirement:• DNA, RNA, cDNA, tissue, cells, bacterial culture

• 0.5µg - 1 µg of RNA for cDNA Synthesis

• Primer Sequence in case of Primer synthesis and designing

• Complete thermal profile of primers

• Standardised primers in case customer is giving the primers.

• Optimal concentration of primers minimum 500nM

Primer Design, Synthesis & Validation

Gene sequence

Oligonucleotide Design (Primer pair designing using

software)

Synthesis of Forward and reverse primer (25-100nM

Concentration)

Optimization of Primers (Thermal profile, Non-specificity)

In-silico Validation



Service Page No.

Cloning 27

Gene Sequencing 33

Primer Designing 83



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Cloning

Cloning of DNA for Sequencing

Sequencing of PCR products using terminal primers (used for

amplification) would usually generate incomplete sequence data,

as it does not read the terminal 50 -100 bases on both ends. iLife

Discoveries offers the service for cloning and sequencing of PCR

products in our standard vector that would enable the scientists to

acquire complete and analysed sequence data of their PCR

products.

Custom cloning service includes designing and synthesis of primer,

PCR and ligation with the vector.

You can give us cells, genomic DNA, RNA and PCR product. This

will be followed by verification of recombinant plasmid by restriction

mapping. Derived clone can be further used for:

• Generation of mutants

• Subcloning

Note: Beyond 1 kb sequence service, each 1 kb will be charged

extra as sample.

Deliverables:1. Clone DNA (up to 5 µg)

2. Sequence data (bi-directional, raw data)

3. Final analysed sequence data

4. Project Report

Precautions and limitations:

If providing PCR product directly:

1. The band of interest should be the most prominent product within

the PCR sample.

2. The samples should not have excessive smearing.

3. Please indicate whether the PCR product has a higher GC –

content



GCL007 Cloning of DNA of < 1 kb with Primers provided

GCL008 Cloning of DNA of 1 kb with Primers provided



GCL018 Cloning DNA of < 1 kb including Primer

designing and synthesis

GCL019 Cloning DNA of 1 kb including Primer









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Cloning and Sequencing of Up-stream/

Down-stream Regions from Known Sequence

The Regulatory Elements of any gene are required for gene

regulation, expression and m-RNA stability studies. Therefore the

Cloning and sequencing of these regions is of great importance.

Promoter, enhancer or 5’-untranslated region is present in up-stream

sequences on the other hand 3’-untranslated region, terminator

sequences etc. are present at down-stream region.

This service will include :• Cloning of up to 2 kb of up-stream or downstream sequences

from known sequence data

• Confirmed bi-directional sequence data

• Putative regulatory regions indicated

Deliverables :1. Cloned DNA containing up to 2 kb of up-stream/down-stream

sequence

2. Bi-directional sequence data (raw data as well as analyzed and

aligned data) with the putative regulatory regions indicated

3. Project report



GCL051 Cloning and Sequencing of Up-stream/

Down-stream Regions from Known

Sequence with Primers provided


Cloning

Cloning of DNA for Expression of Protein

iLife can assist in cloning any gene in any vector for expression of

the protein product. Based on the requirement, the derived protein

can have:

• His Tag

• Maltose binding protein domain

• FLAG

The tag can even be removed on your demand.

Custom cloning services will include designing and synthesis of

primers, PCR, restriction digestion and ligation with the vector.

You can give us cells, RNA, PCR product and Plasmid (in case of

subcloning). This will be followed by verification of recombinant

plasmid by restriction mapping.

Note: Beyond 1 kb sequence service, each 1 kb will be charged as

extra sample.

Deliverables :1. Clone DNA (up to 5 µg)

2. Sequence data (bi-directional, raw data)

3. Final analysed sequence data

4. Project Report

Precautions and limitations :If PCR product is provided:

1. The band of interest should be the most prominent product within

the PCR sample.

2. The samples should not have excessive smearing.

3. Please indicate whether the PCR product has a higher

GC – content



GCL029 Cloning of DNA of < 1 kb for Expression of

Proteins with Primers Provided

GCL030 Cloning of DNA of 1 kb for Expression of






GCL040 Cloning DNA of < 1 kb for Expression of

Proteins including Primer Designing and Synthesis

GCL041 Cloning DNA of 1 kb for Expression of Proteins

including Primer Designing and Synthesis








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Cloning of STRs/ Microsatellite Markers

Genotyping is a technology that can distinguish one sample from

another. The technology takes advantage of the Short Tandem

Repeats (STRs) or Microsatellites that occur dispersed through out

the genomes. The STRs are more often than not polymorphic in

nature and the number of repeats occurring in each STR locus

varies within samples. STRs that are linked/segregate with traits

are used for Market Assisted Selection (MAS) in breeding programs.

Cloning and characterization of STRs that might demonstrate

polymorphism within very similar samples are important for DNA

fingerprinting/ genotyping purposes.

This service will include :• Development of 10 independent STR clones, starting from

samples or genomic DNA (minimum guaranteed)

• Sequencing of the clones, demonstrating STRs

• STR specific primers would be designed (optional)

• Standardization of STR specific “PCR/multiplex PCR” conditions

as well as screening samples for polymorphism are also

undertaken (optional)

Deliverables :1. 10 clones with STRs/ minimum

2. Sequence data and electropherogram

3. Final report

Cloning



GCL053 Cloning of STRs/ Microsatellite Markers

with Primers provided

GCL054 Cloning of STRs/ Microsatellite Markers

including Primer designing and synthesis


Full-length cDNA Cloning Services

Cloning and sequencing of full-length cDNA allows scientists to :

1. Analyse the intron/ exon junctions;

2. Deduce the amino acid sequence coded by the gene;

3. Study the up-stream/ down-stream regulatory regions;

4. Express the gene for protein expression studies etc.

Cloning of full-length cDNA can be achieved from any of the

following information:

• Partial/ full-length cDNA sequence data

• Genomic clone sequence data

• Amino acid sequence data

• cDNA/ genomic DNA libraries

This service will include:• Cloning into any commercially available cloning and/ or

expression vector

• Cloning and sequencing of full-length cDNA irrespective of the

gene size and the number of introns present.

• Fast and reliable service

Deliverables:1. Full-length cDNA into a cloning vector

2. Bi-directional sequence data (both raw data and analysed data)

3. Project report



GCL055 Cloning of Full-length cDNA of 1 kb with Primers provided



GCL065 Cloning of Full-length cDNA of 1 kb including Primer


GCL066 Cloning of Full-length cDNA of 2 kb including

Primer designing and synthesis

GCL067 Cloning of Full-length cDNA of 3 kb

including Primer designing and synthesis



Service Page No.



DNA Analysis 21

RNA Analysis 22


Gene Sequencing 33




Immunological Assays (ELISA & ELISPOT) 64

Biomolecular Interaction Assays 65





Primer Designing 83



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Library construction

Genomic Library Construction

Genomic DNA libraries are commonly used when one wants to

preserve the entire genome of an organism. The library thus formed

will help the scientist to study the entire genome, including large

numbers of non-coding and regulatory regions from the genome.

Custom Genomic DNA library construction service

will include :• Extraction of genomic DNA from a variety of samples

• Generation of phage- or plasmid-based DNA libraries from the

size fractionated restriction digested DNA

• Directional cloning of DNA fragments into any vector

• Production of a library with at least 10-6 cfu

• Library amplification to generate higher titer library

• Verification of library quality by randomly picking clones and

determining insert sizes by restriction digest

• Verification of library complexity by DNA sequencing the inserts

from a subset of randomly picked clones (Optional)

Deliverables:1. Library delivered on plates, filters or glycerol stock

2. Project Report



GLG005 Genomic DNA Library Synthesis




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Library construction

cDNA libraries are commonly used when one wants to reproduce

only the coding regions of the eukaryotic genomes, and the amount

of information is reduced to remove the large numbers of non-coding

and regulatory regions from the library.

Custom cDNA library construction service will

include:• Extraction of Total RNA from a variety of samples

• Generation of phage- or plasmid-based DNA libraries from the

size fractionated cDNA

• Directional cloning of DNA fragments into any vector

• Production of a library with at least 10-6 cfu

• Library amplification to generate higher titer library

• Verification of library quality by randomly picking clones and

determining insert sizes by restriction digest

• Verification of library complexity by DNA sequencing the inserts

from a subset of randomly picked clones (Optional)

Deliverables:1. Library delivered on plates, filters or glycerol stock

2. Project Report



GLG006 cDNA Library Synthesis


cDNA Library Construction



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Site Directed Mutagenesis

iLife offers a complete mutagenesis service for creating single

multiple point mutations, deletions, insertions or random mutations

into any gene of your interest.

Site Directed Mutagenesis helps in :• Study of active sites, structure - function relationships, nucleic

acid - protein interactions etc.

• Construction of fusion proteins and tagged proteins

• Construction of knockout constructs

• Generation of alternative splice forms

• Test of functional elements (promoter regions, terminator

sequences etc.)

Custom Site Directed Mutagenesis service will include :

• Design and synthesis of mutagenesis primers

• PCR amplification

• Confirmation of desired mutations by DNA sequencing

• Confirmation of undesired mutations by DNA sequencing

• Subcloning modified gene into fresh vector not subjected to the

mutagenesis procedure or subcloning into another vector

(Optional)

• Verification of vector sequence after mutagenesis by DNA

sequencing (Optional)

• Delivery of purified plasmid DNA construct

Deliverables :• 4 ug lyophilized destination vector containing the gene

• Sequence chromatograms covering your gene (electronic)• Construct map for the plasmid (electronic)

• Project Report



GSM001 Plasmid <7kb, single point mutation

GSM002 Plasmid <7kb, multiple point mutations

GSM003 Plasmid >7kb, single point mutation

GSM004 Plasmid >7kb, multiple point mutations




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Gene Sequencing

Ready to Run Sequencing

Ready to run sequencing service is provided for the following:

• PCR Products

• Plasmid

• BACs

Sample required is sequence ready purified amplicons & result is

provided in the form of long read length, high quality sequence

data.

Single Pass



GRR001 Ready to run gDNA amplicons

GRR002 Ready to run Plasmid amplicons

GRR003 Ready to run BAC amplicons




GSP001 Single Pass sequencing from Culture plates

(Using Universal Primers)

GSP002 Single Pass sequencing from gDNA (Using Universal Primers)

GSP003 Single Pass sequencing from Plasmid (Using Universal Primers)

GSP004 Single Pass sequencing from RNA (Using Universal Primers)

GSP005 Single Pass sequencing from cDNA (Using Universal Primers)

GSP006 Single Pass sequeincing from cloned vectors


GSP07 Single Pass sequencing from BAC/YAC (Using Universal Primers)

GSP08 Single Pass sequencing from non-purified PCR amplicons


GSP09 Single Pass sequencing from purified PCR amplicons


GSP010 Single Pass sequencing from Culture plates

(Using Custom Primers)

GSP011 Single Pass sequencing from gDNA (Using Custom Primers)

GSP012 Single Pass sequencing from Plasmid (Using Custom Primers)

GSP013 Single Pass sequencing from RNA (Using Custom Primers)

GSP014 Single Pass sequencing from cDNA (Using Custom Primers)

GSP015 Single Pass sequeincing from cloned vectors


GSP016 Single Pass sequencing from BAC/YAC (Using Custom Primers)

GSP017 Single Pass sequencing from non-purified PCR amplicons


GSP018 Single Pass sequencing from purified PCR amplicons



Single-pass sequencing services are provided for the following

applications:

de-novo Sequencing

For initial/primary sequencing of genetic content of organisms.

Samples - Cell culture plates/ ss or dsDNA/ PCR products/ Cloned

vector cells/ Plasmid.

Re-Sequencing

For detection of mutations in candidate genes or genomic regions

of interest.

Samples - Candidate gene PCR product/ whole genomic DNA/

Cell culture plates/ Cloned PCR Product.

SNP discovery

Targeted re-sequencing approach for accurate polymorphism

identification with Sanger sequencing.

High quality sequence reads are provided. Long sequences are

provided with maximum accuracy using long capillaries.


Service Page No.



Plasmid DNA Purification 20


Cloning 27


Gene Synthesis 52

Bacterial Identification 53

Primer Designing 83



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Gene Walking

Gene Walking services for template size ranging from 1 kb to 10 kb

are available for the following.

• PCR amplicons

• Plasmid inserts

• Entire plasmids

• Genomic DNA to produce single strand

• Culture Plates

• BAC or Phage DNA

Beginning from the reference sequence, stage one of sequencing

is started, and then onwards, primers are designed to perform

overlapping sequencing of the remaining DNA and assemble the

sequence data. Additionally, if you have a known sequence for

which you need quality resequencing information, a complete set

of overlapping primers will be designed for generating complete

sequence.



GGW001 Gene walking for upto 1kb










GGW011 Gene walking for more than 10kb


• Upto 4kb of amplicons can be generated.

• Contigs are assembled using refined bioinformatics tools and

high quality amplification & sequencing primers are designed

for Gene walks.

Fragment Analysis

Fragment Analysis services for almost all types of fragments like

AFLP, SNP, SSR, STR, VNTR etc. are provided for the following

applications:

• Micro-satellite Analysis

• AFLP Analysis

• RFLP Analysis

• SNP Analysis

• Mutation Detection

• Microbial Identification (16s rRNA Seq.)

Microsatellite Analysis:

• 6-FAM • VIC • NED • PET • LIZ

For high-throughput analysis, multiplex reactions with primers

labeled with different dyes (5 plex maximum) & fragment sizes can

be performed.

Sample submission can be:• Genomic DNA with primer details

• Pre-labeled primers for multiplexing along with the fragments

and thermocycler conditions

• Primer sequences in excel format with fragments (specifying the

labeling type) and thermocylcler conditions

• PCR amplicon pool of the multiplexed reaction for sequencing

data generation and analysis

Highly accurate data is provided with less than 1bp difference

between the sample replicates.

SNP DetectionSingle nucleotide polymorphisms (SNPs) are widely used as DNA

markers in genetic studies. Compared to other SNP genotyping

methods, sequencing is in particular, suited to identifying multiple

SNPs in a small region, such as the highly polymorphic Major

Histocompatibility Complex region of the genome.

Efficient SNP detection methods on standard DNA sequencers are

available.

Five types of dyes which can be analyzed in each sample are:



GFA001 Microsattelite analysis using unlabelled primers

GFA002 Microsattelite analysis using conventionally labelled primers

(for multiplexing;2 plex)




(for multiplexing;4plex)

GFA005 Microsattelite analysis using conventioanlly labelled primers


GFA006 Microsattelite analysis using custom labelled primers





Gene Sequencing


(for multiplexing;4plex)



GFA010 Microsattelite analysis using 96 well reaction format

GFA011 AFLP analysis

GFA012 RFLP analysis

GFA013 SNP analysis

GFA014 SSR marker identification

GFA015 Mutation detection in Prokaryotic system

GFA016 Mutation detection in Eukaryotic system

GFA017 Microbial identifcation (16s rRNA sequencing)





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DNA Microarrays

Microarrays are now most extensively used and reliable tools for

exploring the transcription events of any living cell. Microarrays can

be a great tool while conducting basic research, understanding

disease mechanisms, exploring stress and environmental tolerance

mechanisms, identifying new drug targets end discovering

biomarkers.

iLife scientists explore a wide variety of genomes across different

model systems using the Agilent’s DNA microarray platform.

Focused on SensitivityHigh-purity, high-fidelity 60-mer oligonucleotide probes deliver better

signal-to-noise-ratios and wider dynamic ranges than other

microarrays.

Engineered for FlexibilityThe SurePrint inkjet technology, enables total control over array

content with no compromise data quality.

Optimized for ScalabilityThe multiplexed arrays have up to eight arrays on a single slide,

making expression studies of any scale feasible and flexible.

With wide range of applications iLife helps scientists enhance their

drug discovery research.

Gene Expression Analysis• Explore transcriptome through expression profiling

• Transcription regulators analysis by microRNA profiling

Location analysis• Explore epigenetic modification by methylation analysis

• Discover protein-DNA interactions through ChIP-on-chip analysis

Copy Number Analysis• Explore the chromosomal variations through Comparative

Genomic Hybridization (CGH)

• Discover DNA Copy Number Variants (CNVs) across the

chromosomes



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DNA Microarrays

Transcriptome Analysis

Gene Expression Analysis

Global gene expression profiling has long been a staple of genomics

researchers as they seek to elucidate cellular function. Unlike

traditional, hypothesis-driven experimentation, global gene

expression studies are often used to identify candidate genes for

follow-on study.

Microarray technology helps researchers explore the transcriptome

and identify & understand low-abundance transcripts and their

influence on biological systems.

We provide gene expression analysis which helps researchers

working on understating the processes of:

• Differential response to toxic agents

• Regulatory points of complex signaling pathways

• Cancer studies

• Difference between treated and untreated samples

• Infectious disease studies

iLife offers end-to-end solutions from sample

preparation to data analysis and deliver

publication ready data.

Genome-wide analysis for human, mouse, rat,

and moreMore than 41,000 genes and transcripts are synthesized as 60-

mer oligonucleotides on the whole-genome slides.

Gene expression profiling can be performed either in one color or

two color experiments.

Gene expression microarrays are available in 4×44K format allowing

advantages of hybridizing multiple samples on single slide, improved

dynamic range and contains Spike-in controls and quality control

reporting for high confidence in hybridization results.

One color gene expressionFor one-color microarray analysis, the individual sample is labeled

and then hybridzed on one microarray. This simple and flexible

experimental setup compares the measured gene expression of a

microarray directly across other microarrays. Data consistency

improves by performing sufficient technical and biological replicate

assays.

Two color Gene expression profilingIn the two-color procedure, two samples are individually labeled

with different fluorochromes such as Cyanine-3 (Cy3) and Cyanine-

5 (Cy5). They are hybridized on the same microarray, allowing a

direct comparison between control versus experimental samples

there by minimizing variability between slides.

Data Analysis

Customized uncompromised high quality publication ready data is

provided in different analysis.



GGE001 Whole Human Genome Microarray One Color

GGE002 Whole Human Genome Microarray Two Color

GGE003 Whole Mouse Genome Microarray One Color

GGE004 Whole Mouse Genome Microarray Two Color

GGE005 Whole Rat Genome Microarray One Color

GGE006 Whole Rat Genome Microarray Two Color

GGE007 Custom 4 x 44K Microarray GE One Color

GGE008 Custom 4 x 44K Microarray GE Two Color

GGE009 Custom 2 x 105KMicroarray GE One Color






GGE015 Gallus (chicken) Genome Microarray One Color

GGE016 Gallus (chicken) Genome Microarray Two Color

GGE017 Arabidopsis Genome Microarray One Color

GGE018 Arabidopsis Genome Microarray Two Color

GGE019 Bovine Genome Microarray One Color



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DNA Microarrays

microRNA Profiling

Discover Novel Pathways of Gene Regulation

The discovery of microRNAs (miRNAs) has revolutionized our

understanding of the regulatory arsenal of the cell. microRNAs are

key regulators of gene expression and have been found to be

modulated in different developmental stages, tissues and disease

states.

iLife offers hybridization based miRNA microarray services to profile

entire human, mouse and custom organism miRNAs.

Hybridization-based microarrays provide maximum flexibility in

terms of species of interest and target coverage.

Entire workflow starting from isolating very difficult microRNA from

various sample sources till the data analysis is performed in well

optimized and clean room facility ensuring high quality and

publishable data. Reliable biological interpretation data will be

provided from the data analysis report.

The flexible format (8x15K) and service can handle projects focusing

on a small set of candidate microRNAs as well as screening studies

designed to profile majority of currently known microRNAs.



GGE061 Human miRNA Microarray

GGE062 Mouse miRNA Microarray

GGE063 Rat miRNA Microarray

GGE064 Custom miRNA Microarray




GGE020 Bovine Genome Microarray Two Color

GGE021 Canine Genome Microarray One Color

GGE022 Canine Genome Microarray Two Color

GGE023 C. elegans Genome Microarray One Color

GGE024 C. elegans Genome Microarray Two Color

GGE025 Corn Genome Microarray One Color

GGE026 Corn Genome Microarray Two Color

GGE027 Drosophila Genome Microarray One Color

GGE028 Drosophila Genome Microarray Two Color

GGE029 E.Coli Genome Microarray One Color

GGE030 E.Coli Genome Microarray Two Color

GGE031 Horse Genome Microarray One Color

GGE032 Horse Genome Microarray Two Color

GGE033 Magnaportha Genome Microarray One Color

GGE034 Magnaportha Genome Microarray Two Color

GGE035 Mosquito Genome Microarray One Color

GGE036 Mosquito Genome Microarray Two Color

GGE037 Pig Genome Microarray One Color

GGE038 Pig Genome Microarray Two Color

GGE039 Rabbit Genome Microarray One Color

GGE040 Rabbit Genome Microarray Two Color

GGE041 Rat Genome Microarray One Color

GGE042 Rat Genome Microarray Two Color

GGE043 Rhesus Monkey Genome Microarray One Color

GGE044 Rhesus Monkey Genome Microarray Two Color

GGE045 Rice Genome Microarray One Color

GGE046 Rice Genome Microarray Two Color

GGE047 Salmon Genome Microarray One Color

GGE048 Salmon Genome Microarray Two Color

GGE049 Sheep Genome Microarray One Color

GGE050 Sheep Genome Microarray Two Color

GGE051 Tobacco Genome Microarray One Color

GGE052 Tobacco Genome Microarray Two Color

GGE053 Xenopus Genome Microarray One Color

GGE054 Xenopus Genome Microarray Two Color

GGE055 Yeast Genome Microarray One Color

GGE056 Yeast Genome Microarray Two Color

GGE057 Yeast Genome Microarray One Color

GGE058 Yeast Genome Microarray Two Color

GGE059 Zebrafish Genome Microarray One Color

GGE060 Zebrafish Genome Microarray Two Color




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DNA Microarrays

Location Analysis

Discover DNA Methylation Patterns

DNA methylation events are heritable modifications that regulate

gene expression without altering the DNA sequence itself. They

serve as regulatory mechanisms for a wide range of biological

processes and genetic diseases, and irregular methylation patterns

have been associated with certain cancers. As a key epigenetic

mechanism, DNA methylation studies are providing researchers

with a critical avenue for exploring key biological processes.

iLife assists scientists discover critical DNA methylation events using

Agilent’s CpG and promoter microarrays. We also customize arrays

that researchers choose their specific regions or proprietary

sequences for additional evaluation. This helps study of regulatory

networks with confidence.

We provide high quality publication ready data according to the

project and offer post service support according to the requirement

of the researcher. Refer page number for detailed description of

the data analysis services.

DNA methylation arrays are available in flexible formats (1x244k to

4x44k) with probes covering the entire genome The Human CpG

Island Microarray contains probes designed with a complete

coverage of the CpG islands & promoters regions about 95kb

upstream and downstream.



GLA001 Human Promoter Set, 2-design(2 slides/array)

GLA002 Human CpG Island Set

GLA003 Human ENCODE Microarray

GLA004 Mouse CpG Island Set

GLA005 Mouse Promoter Set, (2 slides/array)

GLA006 Arabidopsis Microarray

GLA007 C. elegans Microarray

GLA008 Drosophila Microarray

GLA009 Zebrafish Promoter

GLA010 Yeast Whole Genome Set

GLA011 S. pombe Microarray


GLA013 Custom 1x244K DNA Methylation Single Owner



GLA016 Custom 8x15K DNA Mthylation Single Owner

GLA017 SurePrint 1X1M G3 CH3 Custom

GLA018 SurePrint 2X400K G3 CH3 Custom






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DNA Microarrays

Chip-on-chip Analysis

Transcription is a complex process that requires multiple interactions

and orchestrated binding of numerous components. Thus, traditional

gene expression analysis provide only partial glimpses into gene

regulation, whereas ChIP-on-chip reveals a high-resolution map of

transcriptional activities, including activation and repression.

By partnering, we enable you to obtain insightful and robust profiles

of DNA-protein binding and promoter occupancy across entire

genomes.

We offer services on Agilent’s catalog Human ENCODE ChIP-on-

chip Microarray and Human Promoter microarray which specifically

identifies human DNA binding proteins within the ENCODE &

Promoter regions through traditional chromatin immunoprecipitation

(ChIP) pull-down coupled with powerful DNA microarray location

analysis.

Genome-Wide Location Analysis

We help scientists explore the genome and perform location analysis

through ChIP-on-chip microarray featuring a total of ~244,000 60-

mer probes. Probes are spaced every ~100-300 bp across regions

of interest in both coding and noncoding DNA sequences.

Focused microarray designs include human and mouse promoter

sets. Whole genome designs include human, mouse, Drosophila,

C. elegans, and Arabidopsis microarray sets.

Applications of Agilent ChIP-on-chip• Uncover and validate gene regulation and regulatory networks

by comprehensive determination of promoter occupancy.

• Identify and characterize molecular events associated with

processes for transcription, DNA replication and repair, as well

as with chromatin modifications and DNA methylation.

• Elucidate modes of action and potential therapeutic activities of

compounds and target genes by mapping gene regulatory

networks relevant to disease and pathophysiological states.

• Validate and augment existing gene expression data with

authentic binding events.

• Identify, assess, and monitor biomarkers responsive to protein-

DNA binding events to serve as bioassays or toxicant signatures

for toxicogenomics.

• Uncover and profile off-target events as well as validate primary

and secondary effects in screening of candidate compounds,

siRNAs, therapeutics, etc.



GLA021 Human Promoter Set, 2-design(2 slides/array)

GLA022 Human CpG Island Set

GLA023 Human ENCODE Microarray

GLA024 Mouse CpG Island Set

GLA025 Mouse Promoter Set, (2 slides/array)

GLA026 Arabidopsis Microarray

GLA027 C. elegans Microarray

GLA028 Drosophila Microarray

GLA029 Zebrafish Promoter


GLA031 S. pombe Microarray


GLA033 Custom 1x244K ChIP-on-Chip Single Owner




GLA037 SurePrint 1X1M G3 ChIP Custom

GLA038 SurePrint 2X400K G3 ChIP Custom




Data AnalysisHigh quality publication ready data is provided under the

bioinformatics support.



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DNA Microarrays

Copy Number Analysis

Comparative Genomic Hybridization (CGH)

Genomic instability is a classic hallmark of cancer genetic disorders.

Traditional methods of comparative genomic hybridization (CGH)

have enabled rough analysis of chromosomal variations, but are

imprecise and low-throughput. To map chromosomal changes,

aberrations with superior precision, oligo-based array-Comparative

genomic hybridization (aCGH) is a better approach with respect to

genetic diseases.

We offer project based complete solution to the scientist interested

in exploring chromosomal changes in:

developmental abnormalities, disease susceptibility, and

differential drug responses associated with :

• Genetic disorders

• Cancer

• Karyotyping

• Evolutionary Biology

Services are provided on catalog CGH arrays covering entire

Human, Mouse and Rat Genomes in higher precision 1x244k and

2x105k formats that enable better resolution in mapping

chromosomal breakpoints and identifying novel microvariations.

We can also provide deletion analysis on a custom 4x44k array

containing 43,000 probes with an average probe spatial resolution

of 35 kb and an emphasis on the most commonly studied genomic

coding regions and cancer-related genes.

Apart from the catalog arrays project specific arrays can be

customized with enhanced coverage of known genes, promoters,

miRNAs, disease pseudoautosomal and telomeric regions.



GCN001 Human Genome 1 x 244K CGH Microarray Kit

GCN002 Human Genome 2 x 105K CGH Microarray Kit

GCN003 Human Catalog 4 x 44K CGH Microarray Kit

GCN004 Mouse Genome 1 x 244K CGH Microarray Kit

GCN005 Mouse Genome 2 x 105K CGH Microarray Kit

GCN006 Rat Genome 1 x 244K CGH Microarray Kit

GCN007 Rat Genome 2 x 105K CGH Microarray Kit

GCN008 Chicken Genome 2 x 105K CGH Microarray Kit

GCN009 Custom 1 x 244K HD-CGH Microarray,


GCN011 Custom 4 x 44K HD-CGH Microarray

GCN012 Custom 8x15K HD-CGH Microarray

GCN013 SurePrint G3 custom 1x1M CGH array

GCN014 SurePrint G3 custom 2X400K CGH array 2x400K

GCN015 SurePrint G3 custom 4x180K CGH array


GCN017 Cyto Custom 1 x 244K HD-CGH Microarray



GCN020 Cyto Custom 8x15K HD-CGH Microarray




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Copy Number Variation (CNV)

Genetic variation is what makes each human being unique, and

determines our susceptibility to diseases. In recent years,

researchers have utilized SNPs for the identification of genetic

markers for complex and common diseases; however, this approach

has only revealed part of the picture. Copy number variants (CNVs)

are reshaping our understanding of the genetics of a growing list of

diseases, and providing a deep insight into the role that these

variants play in genetic diseases. The use of high-resolution aCGH

microarrays to study CNVs enables researchers to get an extremely

fine view of variant structure and impact.

We use Agilent SurePrint G3 and High definition CNV microarrays

to offer scientists comprehensive genomic coverage that enables

the simultaneous identification and quantification of CNVs at

multiple loci with maximum specificity and sensitivity.

CNV microarrays are available in 1x244k and 1x1M formats

depending on the overall spacing of the tiled probes representing

human coding and noncoding sequences.

We offer complete project based services starting from the DNA

isolation till data analysis and provide publication ready data keeping

in view customers requirements and by providing excellent post

service support.



GCN023 Human Genome 1 x 244K CNV Microarray Kit

GCN024 Human Genome 2 x 105K CNV Microarray Kit

GCN025 Human Catalog 4 x 44K CNV Microarray Kit

GCN026 Mouse Genome 1 x 244K CNV Microarray Kit

GCN027 Mouse Genome 2 x 105K CNV Microarray Kit

GCN028 Rat Genome 1 x 244K CNV Microarray Kit

GCN029 Rat Genome 2 x 105K CNV Microarray Kit

GCN030 Chicken Genome 2 x 105K CNV Microarray Kit

GCN031 Custom 1 x 244K HD-CNV Microarray,


GCN033 Custom 4 x 44K HD-CNV Microarray

GCN034 Custom 8x15K HD-CNV Microarray

GCN035 SurePrint G3 custom 1x1M CNV array

GCN036 SurePrint G3 custom 2X400K CNV array 2x400K

GCN037 SurePrint G3 custom 4x180K CNV array


GCN039 Cyto Custom 1 x 244K HD-CNV Microarray



GCN042 Cyto Custom 8x15K HD-CNV Microarray

GCN043 SurePrint G3 2x400K Human CNV array

GCN044 Human catalog 2x105k CNV Microarray kit


DNA Microarrays



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DNA Microarrays

Custom Microarray Development

Whether studying the genome of a unique organism or digging

deep into a geneset of interest, customization is possible for any

genome, any application, anytime and anywhere.

iLife helps you design microarrays and exclusively customize arrays

for specific applications including :

• gene expression

• microRNA profiling

• DNA methylation

• Comparative genomic hybridization (aCGH)

• ChIP-on-chip

• model organisms

We help you select probe content from wide range of high definition

databases present on Agilent eArray portal. Oligonucleotide probes

are also custom designed from the genes and transcript annotations

present in public databases such as RefSeq, Unigene, TIGR,

GenBank etc. or from the sequences generated by the customer

for specific organism of interest. This allows characterizing the

microarrays with excellent genome coverage linking all essential

genomic and proteomic databases.

We offer the flexibility of choosing any number of genes and

multiplexing the arrays on a single slide (Formats. 4x44k, 8x15k,

2x105k, 1x1M etc.)





GGE009 Custom 2 x 105KMicroarray GE One Color






GGE064 Custom miRNA Microarray





GLA017 SurePrint 1X1M G3 CH3 Custom








GLA037 SurePrint 1X1M G3 ChIP Custom






GCN011 Custom 4 x 44K HD-CGH Microarray

GCN012 Custom 8x15K HD-CGH Microarray

GCN013 SurePrint G3 custom 1x1M CGH array

GCN014 SurePrint G3 custom 2X400K CGH array 2x400K






GCN020 Cyto Custom 8x15K HD-CGH Microarray


GCN032 Custom 2 x 105K HD-NV Microarray,

GCN033 Custom 4 x 44K HD-CNV Microarray

GCN034 Custom 8x15K HD-CNV Microarray

GCN035 SurePrint G3 custom 1x1M CNV array

GCN036 SurePrint G3 custom 2X400K CNV array 2x400K






GCN042 Cyto Custom 8x15K HD-CNV Microarray


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DNA Analysis 21

RNA Analysis 22

Real Time PCR Assays 43

Microarray Data Analysis 76

Custom Chip Designing & Analysis for Microarray 80




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Real Time PCR Assays

Real Time quantitative PCR is used as a quantitative tool for gene

expression nowadays. QPCR is a powerful, highly sensitive

technique that enables both detection & quantitation (as absolute

number of copies or relative amount when normalized to DNA input

or additional normalizing genes) of one or more specific sequences

in a DNA sample. iLife Discoveries supports both Taqman & SYBR

Green chemistries for detection of products in real time PCR.

We use a wide range of fluorescent dye wavelengths for detection

from 350 nm to 700 nm consisting of four dyes FAM, Cy5, HEX

and ROX.

We offer complete services from sample preparation till data analysis

which also include primer and probe designing, synthesis and

validation services.

Real Time QPCR delivers powerful capabilities for generating and

analyzing data for variety of applications:

• Validation of Microarray data

• DNA/RNA Copy number Assessment

• Primer Efficiency determination

• Allele Discrimination

• Load Detection (viral, bacterial or fungal)

• SNP Analysis

• Splice Variant Detection

• Mutational Analysis

• Residual Disease Detection

• Detection of Chromosomal translocations

• Screening of GMOs

• miRNA Detection



GRT001 SYBR-Green based QPCR

GRT002 Taqman-based QPCR

GPR001 Primer Designing, Synthesis and Validation for

SYBR-Green Detection

GPR002 Probe & Primer Designing, Synthesis and

Validation for Taqman Detection


Real Time QPCR Assays

Sample Submission Guidelines

Sample Type Minimum Sample

RNA 500ng-1000ng

Tissue 15-30mg

Bacterial Cells 1.5-2ml( 115 cells/ml)

Mammalian Cells 1.5-2ml( 110 cells/ml)

Whole Blood 1.5 ml

Yeast cells 1.5-2ml( 112 cells/ml)



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Absolute Quantification by qPCR

Real Time qPCR can be used for Absolute Quantification of unknown

samples. It is a method used to quantify unknown samples compared

to samples of known concentration. The Ct values of the unknowns

can now be compared to the standard curve to determine the initial

quantity of template in these samples.

We offer complete solution from sample preparation to data

generation which includes standard curve, Initial template quantity,

Threshold cycle number, amplification plots, plate sample values,

text report.

Our services can be utilized for applications of :

• Viral Load Detection

• Genomic and Mitochondrial DNA Detection (Forensics)

• DNA/RNA Copy number ssessment

• Primer Efficiency determination

Sample submission Requirement :

• Standards for plotting of standard curve

• Unknown samples for quantification




GAQ001 Absolute Quantitation with Standard Curve

GAQ002 Absolute Quantitation without Standard

Curve

Relative/Comparative Quantitation

Comparative quantification of genes is an application of RT-PCR

which is used for calculation of relative abundance of a gene in

treated sample with respect to the control sample in terms of fold

change or copy number.

We offer complete solution from sample preparation to data analysis

which includes Relative Quantity Charts where relative

concentration of gene of interest is measured in unknown sample

compared to the control.

Data is generated in the form of amplification plots, raw fluorescence

values, plate sample values and text report.

We can perform relative quantitation services for applications of :

• Gene Expression

• Microarray data validation

• Pathogen Detection

• GMO Detection

• Environmental Testing

• Infectious Diseases

Sample Submission Guidelines• Calibrator (a positive control of known concentrations that all

samples will be compared for the up-regulation and down

regulation of gene levels)

• Infected samples containing unknown concentration of gene of

interest

• Housekeeping gene required for constant level expression in

calibrator and infected samples.




GRQ001 Relative SYBR Green Quantitation with House Keeping genes

GRQ002 Relative SYBR Green Quantitation without House Keeping genes

GRQ003 Relative Taqman Quantitation with House Keeping genes

GRQ004 Relative Taqman Quantitation without House Keeping genes




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Multiplex QPCR allows one or more targets of interest to be

amplified with a normalizing gene in the same reaction vessel by

using probes with spectrally different fluorophores for the detection

of each amplicon. Generally experimental projects require many

runs on the same set of genes.

iLife provides cost effective and higher level of statistical correlation

to design a multiplex reaction to use for the duration of the study.

The multiplex reaction is important when the template material is

limited as this approach allows maximum amount of data to be

generated from each assay. The primers and probes for multiplex

reactions will be designed the same way as for singleplex reactions

with a few extra considerations.

We offer complete solutions from Sample preparation to data

analysis which includes raw fluorescence data, amplification

plots, threshold, dissociation curve (only in case of SYBR

Green) & standard curve

Applications

• Microbial Diagnostics

• Infectious diseases

• Bacterial,Viral identification



GMR001 Multiplex QPCR Assay


Multiplex Real Time Assay

Validation of Microarray data

Quantitative real-time PCR (qPCR) is a commonly used validation

tool for confirming gene expression results obtained from microarray

analysis.

Microarray analysis performs hundreds or thousands of differentially

expressed genes simultaneously while qPCR provides precise

quantification of a wide dynamic range of expression levels due to

its increased sensitivity and reproducibility. We can rapidly validate

and correlate microarray data through the qPCR approach.

We identify real gene specific variation, as it is important to

normalize gene expression level by carefully selected stable internal

control genes.

Applications

Gene Expression Workflow

Sample preparation and isolation of RNA

Amplification and labeling

Microarray processing and analysis

QRT-PCR validation

Molecular Diagnostics

The real time PCR provides high-throughput method for faster and

more sensitive and more specific methods to diagnose viral

pathogens.

Infectious diseases

The prevalence of disease in patients is exacerbated by inadequate

methods for pathogen detection. PCR-based amplification

approaches have been developed to address this problem because

conventional methods for pathogen identification lack sensitivity,

specificity and speed, and some infectious organisms are difficult

to culture. PCR amplification of ribosomal genes and their internal

transcribed spacer regions coupled with sequence-specific

detection probes are the most reliable approaches for disease

identification.




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Metabolic Disorders

The introduction of real-time PCR provides the opportunity for the

rapid detection of pathogens in food and clinical settings. Apart

from saving time, real-time PCR is highly specific and sensitive

and offers the potential for quantification .The risk of cross-

contamination is significantly reduced, and high-throughput

performance and automation are possible since no post-PCR

manipulations are required.

Child and Women health

The Indian population comprises of a large mass of children and

women who are facing health problems. The introduction of

Molecular Beacon probe and Taqman probe allows more rapid and

specific identification of pathogens.

Primer Efficiency Determination

Primer Efficiency can be easily determined with Standard curve

plotting that would test the run and determine the overall assay

performance.

A dilution series is prepared in triplicates along with the negative

control and a minimum concentration of primer is required for

plotting of the standard curve.

The software also provides dissociation curve that determines the

specificity of primers, the presence of primer - dimer in the wells

and non-specific amplification present if any.

SYBR Green Melting Curve Analysis

·

Primer Optimization Matrix

Optimize concentration of forward and reverse primers and total

primer concentration.

Serial Dilution Standard Curve

Efficiency = [10(-1/slope) ] - 1

DNA/RNA copy number assessment

Bacterial, Viral and Fungal Load Detection

Real time PCR is an appropriate technique for the identification

and quantification of Bacteria viruses and fungus. Emerging Micro-

organisms need to be identified and characterized in terms of the

prevalence in the population and their relevance.

Data Generated:

• Initial copies calculated through Standard Curve plotting

• Plate sample values(Florescence or threshold cycle number)




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Environmental Testing using Labeled Probe

Micro-organism population dynamics assessed by real time

quantitative PCR in the environment. A real time quantitative PCR

(QPCR) is used to detect and quantify the 16S rRNA gene

concentration

A Real time PCR screening is done to detect specific strains of

micro-organisms in environmental samples. Fluorescently labeled

probe are designed against the targets i.e. a short segment of the

micro-organism DNA which is highly specific to the probe

Microbial Diagnostics

Initially, implementation of the polymerase chain reaction (PCR)

resulted in sensitive detection of microbes that were hard or

impossible to diagnose by conventional diagnostic procedures such

as culture and serology. A further improved version is now rapidly

replacing end-point detection PCR in many diagnostic laboratories.

In real-time PCR, amplified products are detected by fluorescence

at the moment that they are generated and directly related to the

input target quantity, so that quantitation is possible.

The probe includes an internal amplification control and primers

and a probe designed to target a highly specific part of the genes.

References:

Applied and Environmental Microbiology, March 2007, p. 1892-

1898, Vol. 73, No. 6

Food and Agricultural

RT-PCR assays have been developed for a variety of target

sequences of food borne microbial pathogens, plant pathogens,

and genetically modified foods. A major and highly sensitive aspect

of RT-PCR is the use of fluorescent reporter dyes that undergo

enhanced fluorescence when bound to DNA. A major advantage of

RT-PCR involves the amplification of short DNA target sequences

of 60-70-bp, which allows greatly reduced extension times, which

when coupled with the advanced technology of RT thermal cyclers

with reduced ramp times, greatly reduces cycle times so that

amplified targets can be recognized within 30 min of amplification

in some cases

Screening of GMOs

Q-PCR is used to measure the quantity of a PCR product. It is the

method of choice to quantitatively measure amounts of transgene

DNA in a food or feed sample.If the targeted genetic sequence is

unique to a certain GMO, a positive PCR test proves that the GMO

is present in the sample.

SNP Analysis Using Real Time PCR

Single nucleotide Polymorphisms (SNPs) are considered to be next

generation markers for the identification of loci associated with

complex diseases and for pharmacogenetic applications. SNPs

allow the unification of the candidate gene approach and

association-based fine mapping to identify gene(s) of interest. They

also aid in the association of linkage analysis to the phenotypic

and genotypic data.

Real Time PCR allows a fast, sensitive and elegant quantification

of genotypes based on SNP. We provide a customized solution for

accurate quantification of SNP allele-specific respective informative

locus analysis using real time PCR. The SNP analysis can be useful

in the following areas:

• Allelic Association

• Linkage Disequilibrium

• Positional Cloning

• Disease Mapping

• Pharmacogenetics

• Mutational Analysis

References

1. Collins A.L., Lonjou C., Morton N.E. (1999) Proc. Nat. Acad. Sci.

96:15173–15177.

2. Lander E.S. (1996) Science 274:536–539

3. Lander E.S., Schork N.J. (1994) Science 265:2037–2048.

Splice Variant Detection Using Real time PCR

Alternative splicing is a widespread process used in higher

eukaryotes to regulate gene expression and functional diversification

of proteins. Investigating the expression levels of splice variants is

an important step for knowing the significance of each variant.

Real Time PCR is a very well suited technique to quantitate

alternatively spliced transcripts. It can use for studying varied

applications:

• Alternatively spliced messenger RNAs

• Exon-specific Quantitation

• Analysis of cytokine mRNA

References

1. Ina I. Vandenbroucke, Jo Vandesompele, Anne De Paepe and

Ludwine Messiaen* Department of Medical Genetics, University

Hospital Ghent-OK5, De Pintelaan 185, 9000 Ghent, Belgium

2. Gibson, U.E., Heid, C.A. and Williams,P.M. (1996)

3. Gilliland, G., Perrin,S., Blanchard,K. and Bunn,H.F. (1990)

4. Kafert, S., Krauter,J., Ganser,A. and Eder,M. (1999)

5. Favy,D.A., Lafarge,S., Rio,P., Vissac,C., Bignon,Y.J. and Bernard-

Gallon,D. (2000)




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Cancer Diagnostics using Real Time PCRCancer-specific Molecular Markers can be used to provide accurate

prognosis and to predict response, resistance, or toxicity to therapy.

The diversity of genomic alterations involved in malignancy

necessitates a variety of analysis for complete tumor profiling in

clinical laboratory. Real-time PCR methods are an attractive option

for the analysis of cancer markers.

It is a rapid, versatile, and cost-effective technique and can be

performed on small tissue samples. Real-time PCR plays an

increasingly important role in clinical testing because it can provide

information about gene expression, gene amplification or loss, and

small alterations (e.g., point mutations). Few applications are listed

below:

• Mutation Detection in solid tumors

• Residual Disease detection

• Molecular diagnostics

References

1. Philip S. Bernarda1 and Carl T. Wittwer1 Clinical Chemistry 48:

1178-1185, 2002.

2. Eleftherios P. Diamandis1 and David E. Bruns2 Clinical Chemistry

48: 1145-1146, 2002.

Real Time PCR in Forensic ScienceQuantification of DNA in a forensic sample is of major importance

for proper DNA amplification and STR profiling. The implementation

of molecular analysis in the forensic laboratory has been a

tremendous benefit to the criminal justice community.

Investigation of forensic samples using real time PCR method helps

in detection with increase specificity and sensitivity. This technology

can be employed in various testing such as:

• Forensic Stain Identification

• Forensic DNA/ RNA Quantification

• Human Identity Testing

• STR Profiling

References

1. Trisha L. Noreault-Conti, Ph.D., and Eric Buel, Ph.D. Vermont

Forensic Laboratory, Department of Public Safety, March 2007.

2. Nicklas JA, Buel E. Anal Bioanal Chem. 2003 Aug; 376(8):1160-

7. Epub 2003 May 9.




GQA001 Validation of Microarray data

GQA002 Molecular Diagnostics for Infectious diseases

GQA003 Molecular Diagnostics for Metabolic disorder

GQA004 Molecular Diagnostics for Child and Women Health abberations

GQA005 Primer Efficiency Determination by SYBR

Green Melting curve analysis

GQA006 Primer Efficiency Determination by Primer optimization Matrix

GQA007 Primer Efficiency Determination by Serial Dilution Standard Curve

GQA008 DNA/RNA copy number assessment

GQA009 Environmental testing using labeled probe

GQA010 Microbial Diagnostics

GQA011 Screening of GMOs

GQA012 Allelic Association detection

GQA013 Linkage Disequilibrium profiling

GQA014 Positional Cloning determination

GQA015 Disease Mapping

GQA016 Pharmacogenetics application

GQA017 Mutation Analysis

GQA018 Splice Variant Detection Using Real time PCR

GQA019 Cancer Diagnostics using Real Time PCR

GQA020 Forensic Stain Identification

GQA021 Forensic DNA/ RNA Quantification

GQA022 Human Identity Testing

GQA023 STR Profiling

GQA024 Customized Realtime PCR Assays



Service Page No.

DNA Microarrays 35

RT-PCR Data Analysis 81




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Next Gen Sequencing

Next Generation Sequencing is changing the genetics landscape

and opening new avenues to expand research and development.

We offer customized solutions for a full range of next Gen

sequencing applications such as :

• Whole genome sequencing

• mRNA/Transcriptome sequencing

• ChIP sequencing

• miRNA and Small RNA discovery

• DeNovo Sequencing

• SNP Discovery

• Sequence capture

• Targeted Resequencing

• Multiplexing sequencing

Today’s scientific evnvironment, both commercial and academic,

necessitates moving beyond studying markers and genes to full

genome, transcriptome and epigenome characterization. Next Gen

even enables previously impractical experiments such as

environmental, population and metagenomics.

While this technology offers new promise to life science and medical

researchers, it also creates complex challenges in data analysis.

We offer excellent bioinformatics support in analyzing terabytes of

data and providing satisfactory and publishable results.

Next Gen sequencing is a project based customized service. Please

contact us to have a detailed discussion on the technical details

and the workflows before deciding on the type of sequence to be

performed.

Whole Genome SequencingWhole genome characterization by shotgun sequencing can be

performed both by single pass fragment analysis and also by paired

end analysis f fragments from total DNA, PCR products, etc.

Fragment reads are used to detect SNPs and small indels in

previously sequenced genomes. Paired End reads are used for

confident genome assembling and to detect larger structural

variants, including chromosomal rearrangements and large indels.

Fragment libraries are typically constructed from 5 micrograms of

total DNA.

Fragment and Paired End analysis will be performed

from 5 micrograms of total DNA

Whole Transcriptome AnalysisThis service includes Quantitative transcriptome profiling by

sequencing cDNAs constructed from messenger RNA isolated from

total RNA.

By sampling randomly from total RNA, RNA-Sequencing yields

counts that are as quantitative as qRT-PCR but across a space

more broad than a microarray - the entire transcriptome!

RNA-sequencing can be combined with Paired-Ends for sequencing

ranscriptomes from non-model organisms for which no reference

is available. Even if you do not need the expression data, RNA-

sequencing can also be a more cost effective strategy for

sequencing the gene space of an organism not previously

sequenced.

RNA-sequencing profiles are also more versatile than microarray

expression profiles as they are amazingly reproducible and can

represent an absolute rather than relative quantitation. In this way,

data sets produced over many runs can be easily compared. Further,

it is possible to make gene-to-gene comparisons instead of only

treatment-to-treatment comparisons of the same gene given by a

microarray.

iLife can provide Next Gen Sequencing service with

just an input of 10 micrograms of total RNA



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Micro RNA Analysis

MicroRNA sequencing is performed for the Discovery & quantitation

of novel microRNAs and isoforms by sequencing cDNAs of

microRNAs isolated from total RNA.

Similar to RNA-sequencing, microRNA sequencing samples

quantitatively from the entire small RNA population of the cell,

including most RNAs smaller than tRNAs. From this data we can

simultaneously discover the expressed microRNA population of

the sample as well as build an expression profile that can be

compared to other samples.

We will need just 20 micrograms of total RNA and the following

steps of microRNAs extraction, cDNA preparation, library

construction, sequencing and analysis will be performed by us.



GNS001 Whole Genome Sequencing

GNS002 Whole Transcriptome Sequencing

GNS003 ChIP Sequencing

GNS004 miRNA Sequencing

GNS005 Customized Next Gen Sequencing Services


Next Gen Sequencing

ChIP sequencing

Unlike the ChIP-on-chip microarrays here is a technology that

involves Discovery & quantitation of protein-DNA interactions by

sequencing DNA from immunoprecipitations.

If the DNA is recovered by immunoprecipitation, we can sequence

it! We offer sequencing IP products from transcription factors,

histone modifications, and RNA binding-proteins.

Using specific antibodies, ChIP-sequence protocols call for the

cross-linking of DNA/RNA with neighboring proteins, sonicating the

fragments until they are very small (~200bp), and then purifying

the DNA/RNA.

In this way we can define the actual binding locations of proteins

genome-wide. Alternatively, we can provide whole-epigenome

profiling by sequencing the ChIP products of one or many histone

modifications.

We offer the ChIPsequence service with any total amount of DNA

but including at least 20 nano-grams of DNA in the 150-250 bp

size range.



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Next Gen Sequencing

(Illumina Single-End Sequencing Platform Library Prep)

SureSelect Target Enrichment System significantly improves the

cost- and process-efficiency of next-generation sequencing. Based

on an extremely efficient hybrid selection technique, the SureSelect

Target Enrichment System can be automated and easily scaled to

meet the needs of larger sequencing projects. This is unlike any

other commercially available target enrichment method. With

sample input requirements at or below 3 µg of gDNA, researchers

can perform highly targeted next-generation sequencing on the most

precious of samples.

SureSelect Target Enrichment System Workflow

SureSelect platform currently supports target enrichment on both

the Illumina Genome Analyzer and the Applied Biosystems™ SOLiD

System. Protocols and kits have been optimized for in-solution works

that are custom designed, along with a catalog product targeting

human exome.

The platform currently consists of the following offerings:

1. SureSelect Target Enrichment System: A custom, in-

solution method useful for large, higher throughput studies.

2. SureSelect All Exon Kits: A single tube assay that targets all

human exons with significant depth of coverage. 1.22% of human

genomic regions are covered, corresponding to the CCDS exons.

3. SureSelect All Exon PLUS Kits: Now you can add your own

custom content! The design covers 38Mb (CCDS + >1,000 ncRNA)

and you can add up to 6Mb of your own content

SureSelect Target Enrichment System



GST001 Customized SureSelect Target Enrichment System



Service Page No.



DNA Analysis 21

RNA Analysis 22



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GGS001 Eukarotic gene synthesis (including codon

optimization; standard delivery ~4µg)

GGS002 Eukarotic gene synthesis (including codon

optimization; custom delivery >5µg)

GGS003 Eukarotic gene synthesis (excluding codon


GGS004 Eukarotic gene synthesis (excluding codon

optimization; standard delivery >5µg)

GGS005 Prokaryotic gene synthesis (including codon


GGS006 Prokaryotic gene synthesis (including codon


GGS007 Prokaryotic gene synthesis (excluding codon


GGS008 Prokaryotic gene synthesis (excluding codon


GGS009 Custom Plasmid preparation of

Research grade (Predominantly supercoiled)

GGS010 Custom Plasmid preparation of SC grade

(e” 95% Supercoiled, d” 0.03 EU/ug Endotoxin)

GGS011 Custom Plasmid preparation of Advanced SC

grade (e” 95% Supercoiled, d” 0.005 EU/ug Endotoxin)

GGS012 Other customized gene synthesis services


Gene Synthesis

Steps• Oligo synthesis

• Obtaining full-length genes

• Mutation correction

• Sequence confirmation

Our codon optimized genesIt matters as much as protein expression matters. Different

organisms have different codon usage preferences. For example,

the preferred codons used in a human gene may be rare in bacteria.

This can cause problems when researchers attempt to express

human genes in E. coli. Using our optimized synthetic genes, many

of our customers have reported dramatic increases in protein

expression.

Why use gene synthesis instead of PCR cloning?

PCR cloning, the most common method of obtaining genes, may

not be able to produce a gene of sufficient quality or even at all.

First, a cDNA library for a specific type of tissue has to be prepared

or purchased, which requires either time or expense. Second, the

gene must be abundant in that cDNA library or it will be very difficult

to clone any useful way. Third, when PCR is successful, its products

may have mutations or single nucleotide polymorphisms (SNP),

which can cause problems during experimentation or other

applications.

Key Features• There is no length limit. We routinely synthesizes genes of 10

kb or longer.

• Standard vector: pUC57; synthetic genes will be cloned into

Sma I or EcoR V sites of the standard vector.

• We can subclone the synthetic gene into the vector of your

choice; the vector must be provided by the customer and must

be a commercial vector with a full-length sequence.

• We can synthesize the 5'-end or 3'-end of the gene and anneal

it to your partial clone to obtain the full-length gene, provided

that you have the sequence.


Service Page No.


Cloning 27

Gene Sequencing 33









Primer Designing 83



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Bacterial Identification

Bacterial Species identification is challenging because of the huge

diversity of morphologically complicated organisms. 16S rRNA

sequencing is one of the most accurate and validated methods for

taxonomical identification of species.

We provide services for complete workflow starting from DNA

isolation to 16S rRNA sequencing and in-silico testing services for

taxonomical identification.

We offer the following services:• Identification of a bacterial strain and taxonomical classification.

• Taxonomical confirmation of the bacteria.

• Comparative analysis of the bacteria with closely related species.

• Phylogenetic arrangement of the bacteria.

• Comparison of the bacterial sequence with the Genbank

database through multiple sequence alignment and identify

known and novel bacteria.

• Mining known bacteria from a mixed population by specific

primers.



GPD001 Primer designing, synthesis and optimization

GBI001 Bacterial Identification without cloning

GBI002 Bacterial Identification with cloning




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Proteomics &Cell Biology

Simple Solutions-Complex Proteins



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Protein Analysis

Protein Extraction & Purification

It is a difficult task to extract Proteins in good amount, from many

tissues. iLife offers to extract & purify highly pure protein in small

and large-scale. We have been routinely extracting considerable

amount of protein, for various proteomics applications, from various

sources including tissue (fresh and preserved), blood, saliva, other

body fluids, cell cultures, plants, and forensics samples.

Protein AnalysisAgilent 2100 Bioanalyzer is used which is a microfluidics based

platform for sizing, quantification and quality control of Proteins.

For detailed technical information kindly refer to the page no:14.

Different kits allow Protein quality control – detection at ng & pg

level:

Protein 250 kit:

It detects proteins down to 1 pg/uL on chip, which is superior to

silver stain SDS-PAGE. It provides quantification over a dynamic

range of up to 4 orders of magnitude, is highly reproducible,

which is particularly important for the analysis and quality control

of proteins.

Protein 230 and Protein 80 kits:

It can be used to:

• Analyze a wide range of of samples

• To express recombinant proteins

• Purify proteins

• Perform stability studies

• Check antibody quality down to 10 ng/µl.


His-tag protein purification with Ni++ ZipTips® Enzymatic removal

of His Tags from recombinant proteins Complementing RP-HPLC

protein purification

Antibody Analysis

Analysis of antibodies under reducing and non-reducing conditions

Quantitation of the half-antibody content in IgG4 preparations

Comparison of SDS-PAGE, CGE and Agilent 2100 bioanalyzer for

humanized monoclonal antibody analysis Absolute quantitation of

IgG Quality control of stressed antibodies Separation of bispecific

antibodies chains

Food Analysis

Bovine milk analysis Protein pattern of different transgenic seedlines

Protein - Others

Absolute protein quantitation Glycoprotein sizing Protein quality

control prior to MS-analysis Depletion of high abundant proteins

from blood samples Increased sensitivity by desalting protein

samples

Note: We offer Customized solutions for Protein applications. Please

contact technical support for further assistance.

PPA001 Analysis of cell lysates - protein induction

PPA002 Comparison between lysate and flow through

PPA003 Analysis of protein purification

PPA004 GFP Streptag fusion protein purification

PPA005 Analysis of column capacity

PPA006 Analysis of column fractions to optimize conditions

PPA007 Analysis His-tag protein purification with Ni++ ZipTips®

PPA008 Analysis of Enzymatic removal of His Tags from recombinant proteins

PPA009 Complementing RP-HPLC protein purification

PPA010 Analysis of antibodies under reducing and non-reducing conditions

PPA011 Quantitation of the half-antibody content in IgG4 preparations

PPA012 Comparison of SDS-PAGE, CGE and Agilent 2100

bioanalyzer for humanized monoclonal antibody analysis

PPA013 Absolute quantitation of IgG

PPA014 Quality control of stressed antibodies

PPA015 Separation of bispecific antibodies chains

PPA016 Bovine milk analysis

PPA017 Protein pattern of different transgenic seedlines

PPA018 Absolute protein quantitation

PPA019 Glycoprotein sizing

PPA020 Protein quality control prior to MS-analysis

PPA021 Depletion of high abundant proteins from blood samples

PPA022 Increased sensitivity by desalting protein samples

PPA023 Customized Protein Analysis


Ordering Information :


PPE001 Protein Extraction & Purification from Tissue

PPE002 Protein Extraction & Purification from Cell Lysate

PPE003 Protein Extraction & Purification from Culture Supernatant

PPE004 Protein Extraction & Purification from Blood Plasma

PPE005 Protein Extraction & Purification from Body Fluids

PPE006 Customized Protein Extraction & Purification Services


Protein ApplicationA specific chip design allows the analysis of upto 10 protein samples

in the 5-250KDa size range with the use of different kits. A variety

of different samples for can be used such as column fractions,

purified proteins, antibodies and cell lysate.

Protein Expression

Analysis of cell lysates - protein induction

Protein Purification

Comparison between lysate and flow through Analysis of protein

purification GFP Streptag fusion protein purification Analysis of

column capacity Analysis of column fractions to optimize conditions


Service Page No.


2D Gel Electrophoresis 60



Pathway Focussed Protein Profiling 63


Biomolecular Intercation Assays using Octet 65





Protein Structure Prediction 84

Antibody Microarray Data Analysis 85




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Protein Expression & Purification

Customized protein expression

iLife can help you select expression system and strains, develop

standard protocols, and scale up processes for expression of

proteins from:

Bacterial protein expression systems: They are both the most

universal and the most cost-effective means of protein expression

available now-a-days.

Yeast Expression System: This system is extremely useful for

expression and analysis of eukaryotic proteins. Protein expression

in yeast is genetically well characterized and is known to perform

many post-translational modifications, grow quickly in defined

medium. It is easy and less expensive to work with, as compared

to insect or mammalian cells & can be adapted to fermentation.

Mammalian Expression Systems: These systems ensure

appropriate post-translational modifications (e.g. disulfide bonds,

glycosylation, phosphorylation) in case of a mammalian protein and

are therefore, the preferred exression systems in case of mammalian

proteins. It is also required for therapeutics & vaccines production,

hybridoma screening etc.

At iLife we:• Will work with your specifications for quantity, varying size,

bioactivity, structure, solubility, post-translational modifications,

compartmentalization (cytoplasmic/periplasmic) and purity, to

express proteins that meet your specialized requirements.

• Will also do small scale induction to over-express target protein

& carry confirmation test for expressed protein by SDS-PAGE

and western blot (customer must provide appropriate antibodies).

• Amplify/isolate gene of interest out of a customer-supplied vector

and subclone it into a suitable promoter based high-level

expression vector or a retroviral vector.

Deliverables :1. Construct(s) report

2. Expression data

3. Expression positive clones (glycerol stock)

4. SDS-PAGE

5. Western Blot data

6. QC data at each step

7. Project report

Ordering Information :


PEP001 Expression and Purification of Protein from Bacteria

PEP002 Expression and Purification of Protein from Yeast

PEP003 Expression and Purification of Protein from Mammalian cells




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Epigenetic expression of proteins under

inducible promoters

It is quite difficult to express toxic proteins, as they tend to destroy

the cell producing them. iLife can help you select the inducible

expression system and strains, develop standard protocols, and

scale up processes for expression of proteins from:

1. Bacterial protein expression systems

2. Yeast Expression System

3. Mammalian Expression Systems

At iLife we• Besides working on your specifications for the quantity, varying

size, bioactivity, structure, solubility, post-translational

modifications, compartmentalization (cytoplasmic/periplasmic)

and purity, to express proteins, we will also work with choice of

inducible vector that meet your specialized requirements for even

toxic proteins.





and subclone it into an inducible promoter based high-level


Deliverables:1. Construct(s) report

2. Expression data


4. SDS-PAGE



7. Project report



PEP004 Epigenetic Expression of Protein under inducible

promoters from Bacteria


promoters from Yeast


promoters from Mammalian cells



Small Scale protein purification for proof

of concept studies

iLife Discoveries is a final solution for your needs, and helps you to

overcome your cloning, protein expression and purification

bottleneck We offer protein purification services for cDNA cloned

into bacterial, mammalian or insect cells. Recombinant protein

production is a core activity of groups involved in high throughput

screening functional proteins for validation and structural biology.

The key to achieving efficient and robust purification procedures is

selection of the most appropriate chromatographic resins for each

separation step comprising a multidimensional process, the

sequence of which is critical for optimization of chromatographic

performance in terms of simplicity, scalability and product yield

At iLife we• Will work with your specifications for quantity, varying size,











2. Expression data


4. SDS-PAGE


6. Purified soluble tagged/untagged protein in lyophilized form


8. Project report



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Customized protein purification platforms for

crystallography studies

At iLife, proteins are usually purified with different columns which

ensure “crystallographic grade” purity. The purity of final products

is better than 95% and can be directly used for crystallization and

other high quality assays.

At iLife we• Will work with your specifications for quantity, varying size,













PEP010 Purification of 15-20mg protein from Bacteria

PEP011 Purification of 15-20mg protein from Yeast

PEP012 Purification of 15-20mg protein from Mammalian cells



2. Expression data


4. SDS-PAGE

5. Western Blot data6. Purified soluble tagged/untagged protein in lyophilized form


8. Project report


Service Page No.

Protein Analysis 56



Antibody Microarray 61

Pathway Focussed Protein Profiling 63











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2DGel electrophoresis is the most widely accepted method for

fractionation of complex mixture of proteins into discrete

components. iLife Discoveries offers high-throughput, two-

dimensional gel electrophoresis for a diverse range of sample types.

One may like to do 2D gel electrophoresis in various studies like:

• Protein Biomarker discovery

• Protein species and isoform distribution analysis

• Analysis of posttranslational modifications

• Differential protein pattern analysis

• Drug mechanism of action

• Predictive toxicology

In this technology, proteins are firstly separated according to their

isoelectric point (pI) in the horizontal direction (isoelectric focusing

[IEF]) followed by separation in the vertical direction [SDS - PAGE],

according to the molecular weight.

After performing various staining protocols, depending on the

specific sample requirement, spots are obtained on gels, which

correspond to different proteins and are isolated and subjected to

enzymatic digestion for further mass spec analysis. At iLife

Discoveries we do in-gel tryptic digestion (as well as in-solution

digestion for whole proteome) of spots excised from 1D and 2D

gels.

Sample requirements :• Total protein: > 500ìg/sample, low salt (< 50 mM), without ionic

detergents

• Cells: > 5x106 cells/sample, 3x washed and centrifuged, 4°C,

pellet flash frozen

• Tissue: >10 mg blood free tissue, washed by PBS, 4°C, flash

frozen

Deliverables :• Project Report

Two - Dimensional Gel Electrophoresis,

followed by MALDI-TOF/TOF

Protein Profiling



POG001 2D Gel Electrophoresis of Bacterial Protein (Low Resolution)

POG002 2D Gel Electrophoresis of Bacterial Protein (High Resolution)

POG003 IEF OffGel Fractionation, for Differential Protein Expression

(Low Resolution)

POG004 IEF OffGel Fractionation, for Differential Protein Expression

(High Resolution)

POG005 IEF OffGel Fractionation, with Digital Analysis of Protein Fractions

(Low Resolution)

POG006 IEF OffGel Fractionation, with Digital Analysis of Protein

Fractions (High Resolution)

POG007 Customized 2D Gel Electrophoresis Services

POG008 Customized OffGel Fractionation Services


Offgel Fractionation

2D-GE is a powerful method, but in case of complex protein

mixtures, many times, people are unable to get reproducible

fractionation with high resolution. In contrast to conventional

isoelectric focusing, OFFGEL fractionation delivers sample in

the liquid phase which is much more convenient for the other

subsequent separation experiments like liquid chromatography.

The main advantages are that there is no need for gel staining

and no need to recover sample from the gel, which decreases

the loss of sample and increases the reproducibility of the results.

The OFFGEL electrophoresis reduces sample complexity,

making it possible to detect a small difference in the protein

pattern.

A combination of OFFGEL fractionation based on isoelectric

point, and high-sensitivity on-chip electrophoresis with the Agilent

2100 bioanalyzer, results in an analytical 2D-GE-type analysis

with excellent sensitivity and reproducibility. Best part of

combination of both technologies (OFFGEL and on-chip

separations) allows detection of even a small change in protein

pattern.



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Antibody Microarray

The Ab Microarray 500 enables to detect a wide variety of proteins

(both cytosolic and membrane bound) representing a broad range

of biological functions.

The Ab Microarray 380 - Disease Profiling Array enables to detect

many different proteins that are known to be associated with a

variety of conditions such as:

• Profiling Disease states

• Cancer research

• Identification of Tumor-associated Antigens

• Metastasis

• Multiple sclerosis

• Parkinson’s

• Alzheimer’s

• Obesity

• Transplanted organ rejection

• Digestive disorders

• Time course studies

• Biomarker discovery

• Target validation

• Target Identification

• Serum Analysis in Human, Mouse & Rat Tissues

Examples of the Ab Microarray’s utility include the study of

expression of apoptosis regulatory proteins, as well as the study of

expression of proteins relevant to diabetes. This array allows to

detect differences in protein abundance between two individual

samples—cells, whole tissue, or biological fluids. The fluorescence-

based procedure, which takes less than a day to complete, lets

you detect as little as 20 pg/ml of each protein target. A dual-color

detection method is uniquely designed so that inherent variations

in dye labeling do not affect the outcome of the experiment Thus,

one can be confident that the side-by-side comparisons reflect the

relative abundances of proteins in the sample. Follow-up studies

with Western blotting and in situ hybridization support these claims.

The proprietary process ensures that antibodies remain functionally

active even after they are covalently immobilized to the glass

surface. The arrays are printed on 75 x 25 x 1 mm glass slides, all

arrayed antibodies are carefully tested for specificity and sensitivity.

Those that display a high degree of cross reactivity are eliminated

from the final product.

Protein Profiling

Figure 1: Antibody Microarray Workflow

Highlights of the Antibody Array:

• 507 antibodies printed on one slide (Figure 2).

• 380 antibodies printed on one slide.

• Each monoclonal antibody is printed in replicates

• Antibodies are covalently immobilized on a standard glass slide

(Figure 2)

• Antibodies have high specificity and affinity

• Slides have low background

• Sensitive Cy3/Cy5 fluorescent detection

• Platform compatible with most commercially available microarray

scanners (75 x 25 x 1 slide)

ProcedureThe antibody array protocol, as followed at iLife Discoveries,

outlined in Figure 3, is a fluorescence-based procedure in which

antibodies printed on a glass surface are used to capture

fluorescently-labeled antigens. The entire procedure, from sample

preparation to array scanning, takes one day to complete.

Figure 2. Layout of the Ab Microarray 500. The Ab Microarray 500 contains at

least 500 distinct antibodies arrayed in a 32 x 32 grid on a 75 x 25 x 1 mm glass

slide (Panel A). Each antibody is printed in duplicate. Panel B. Darker dots at the

corners represent Cy3™/Cy5™-labeled bovine serum albumin (BSA) spots, which

serve as orientation markers. The open circles correspond to unlabeled BSA spots,

which serve as negative controls. The Ab Array 380 has a similar layout - rows 5

and 6 are not printed.

Figure 3. Flowchart of the antibody array procedure.



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PAM001 Protein Profiling on Antibody Microarray 500

PAM002 Custom Antibody Microarray

PAM003 Custom Reverse Phase Microarray


We provide complete solution for biomarker discovery starting from

experimental plan, target identification, target validation abd

population screening for the biomarker on Antibody Microarray 500

with salient features.

• We analyze over 500 native proteins in one experiment

• We can compare protein expression levels among cells, tissues,

and biological fluids

• We offer internal normalized results enhancing assay precision

and accuracy

• We can detect a wide variety of proteins (both cytosolic and

membrane bound) representing a broad range of biological

functions, including signal transduction, cell-cycle regulation,

gene transcription, and apoptosis.

Customized services include development of microarray with

selected set of antibodies either from the database or customer

owning antibodies.

Our expertise in the technique walks scientist through a complete

project of identifying the antigen in disease states through Ab

microarray and later screen the population through Reverse Phase

Microarray.

Reverse-phase Protein Lysate microarrays (RPAs) provide an

exciting new platform for measuring protein expression levels in a

large number of biological samples simultaneously. Proteomic

profiling using RPAs yields more direct answers to functional and

pharmacological questions than transcriptional profiling.

Data AnalysisCustomized data analysis will include identifying the candidate

antibodies showing differential expression and categorizing them

depending on their function.

Data Validation

The purpose of the antibody array is to provide a reliable, easy-to-

use tool that generates reproducible results. Antibody array results

can be validated by Western blot analysis, ELISA,

immunohistochemistry, or literature searches. The Western blot

analysis and array data show an 80% or better correlation..

Protein Profiling



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Pathway Focussed Protein Profiling

Antibody Microarrays provide a high-throughput platform for

sensitive, efficient and accurate protein expression profiling

Population screening, comparison between normal, diseased

or treated samples and identifying candidate biomarkers.

Apart from the whole proteome sequencing we also help scientists

screen their clinical samples to explore the disease specific and

pathway specific proteins with our custom made focused antibody

microarray services.

Services are available for studying phosphorylation events in cell

and tissues and wide range of pathways involved in :

• Angiogenesis

• Cancer markers

• Cytokines

• Signal Transduction

• Apoptosis

• Cell Cycle

• Hormones

• Stem Cell etc.

Proteins from cell extracts, fresh, frozen and FFPE tissues or serum

samples can be analyzed at our facility with utmost accuracy and

deliver publication ready results.

Data AnalysisCustomized data analysis will include identifying the candidate

antibodies showing differential expression and categorizing them

depending on their function.

Protein Profiling



Explorer Arrays

PPP001 Kinase Antibody Microarray

PPP002 Phospho Explorer Antibody Microarray

PPP003 Tyrosine Phosphorylation Pro Array

PPP004 Protein Explorer Array

Phosphorylation Antibody Arrays

PPP005 AKT Pathway Phospho-Specific Microarray

PPP006 AMPK Signalling Microarray

PPP007 Cancer/Apoptosis

PPP008 Cell Cycle

PPP009 Chromatin/Transcription

PPP010 Cytoskeleton

PPP011 EGF Pathway

PPP012 ERK signalling

PPP013 GPCR signalling II

PPP014 MAPK/ERK

PPP015 Insulin Receptor Pathway

PPP016 GAK/STAT

PPP017 MAPK Pathway

PPP018 Neuroscience

PPP019 NF/KB II Signalling

PPP020 p53 signalling

PPP021 TGF/beta signalling

PPP022 Tyrosine Kinase Adaptors

Pathway Antibody Microarrays

PPP023 Angiogenesis

PPP024 Cancer Markers

PPP025 Cytokines

PPP026 Signla Transduction

PPP027 Apoptosis

PPP028 Cell Cycle

PPP029 Hormones

PPP030 Stem Cells



Service Page No.

Protein Extraction and Purification 56












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ELISA

The Enzyme Linked Immunosorbent Assay (ELISA) is a common

assay used to check the presence of particular antigens or

antibodies.

We offer a wide range of ELISA services which include:

• Broad range screening of cytokines, antigens, signaling

molecules, etc.

• Test of various disease related antigens and plant molecules.

• Designed or synthesized peptides/ antigens which can be

screened against wide range of antibodies

Deliverables :1. Project report

Immunological assays

ELISPOT

The Enzyme-linked Immunosorbent SPOT (ELISPOT) is unlike

ELISA where secreted analyte from stimulated cell is captured by

labeled antibody for assay.

Immune cells after interacting antigens secrete number of

stimulating chemicals like cytokines. The ability to measure this

response is vital for drug discovery, basic research and

management of a range of important diseases. This response

readout at single-cell resolution is required to develop better

understanding and treatment of allergies and autoimmunity, to

predict allograft rejection in organ transplantation, for improvements

in drug and vaccine development, and for monitoring various cancer

treatments.

iLife offers a wide range of ELISPOT services which

include :• Broad range screening of cytokines, antigens, signaling molecules, etc.

• Test of various disease related antigens and plant molecules.

• Designed or synthesized peptides/ antigens which can be

screened against wide range of antibodies

Deliverables :1. Project report



PIM001 ELISA




PIM002 ELISPOT




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Biomolecular Interaction Assays

iLife offers highly customized assays for investigating biomolecular

interactions. The assays are versatile over a range of interactions

including :

1. Antibody-antigen

2. Receptor – Ligand

3. DNA - DNA

4. DNA - Protein

5. Antibody Fragment - Antigen

6. Antibody - Peptide

7. Multiple Antibody Pairings

8. Antibody - Small Molecule

9. Protein - Small Molecule

Monitoring antibody concentrationiLife is a leading provider to the research and Industrial community

of a high-end technology platform for monitoring antibody titres.

On receiving your established hybridomas, we continue to grow

them in our cell culture facility and monitor the titer values on a

regular basis on the Octet Red. Conditions of growth can be altered

as required to be able to achieve larger antibody titre values.

Alternatively popular packages allow for the

quantification of antibody production in

periodically delivered customer samples

Small Molecule Detection

Investigating the affinity of a small molecule to bind to a therapeutic

target is a significant step in the drug discovery process. The Octet

RED System is designed to enable the detection of small molecules

using Super Streptavidin Biosensors. Real-time, label-free analysis

of the association and dissociation of a small molecule with the

target biotinylated protein of interest results in the determination of

kinetic constants furnishing pharmacologically relevant data.

High Affinity:High Affinity:High Affinity:High Affinity:High Affinity: Carbonic Anhydrase:Azetazolamide (222

Daltons)

Figure 3: Analysis of a high affinity interaction (KD= 39 nM): acetazolamide/carbonic

anhydrase binding data at 1- 0.012 ìM acetazolamide (3X dilution series) in PBS

with 0.5% DMSO.

1. Papalia et al, Analytical Biochem 359 (2006), 94-105.

2. Myszka, Analytical Biochem 329 (2004), 316-323.

Kinetic profiling of immunotherapeutics

Immunotherapeutic agents use or modify immune mechanisms.

Use of these agents is rapidly growing. New classes, new agents,

and new uses of current agents are constantly in the development

pipeline. We can customize experimental protocols to measure on

and off rates of immunotherapeutics. Eight samples can be analyzed

in parallel.



PBI001 Biomolecular interaction assay for Monitoring

antibody concentration

PBI002 Biomolecular interaction assay for Small Molecule Detection

PBI003 Biomolecular interaction assay for Kinetic profiling of

immunotherapeutics

PBI004 Biomolecular interaction assay for Concentration

measurements based on binding rates


Concentration measurements based on binding rates

Concentration measurements can be made using the binding rates

of test samples which are interpolated from the standard curve.



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Protein Quantitation in Bioprocessing

Our Technologies provide researchers with label-free solution for

antibody quantitation. Pre-coated anti-hIgG, anti-murine, and Protein

A Biosensors are ready to use in quantifying a variety of IgG

isotypes. Streptavidin-coated and Amine Reactive Biosensors

expand your quantitation options to include all proteins in developing

custom protein assays. We guarantee accurate results on

customized workflows resulting on good quality publishable data.

Now you can quantify antibodies or other proteins

that are critical to:• Early cell culture screening

• Protein purification

• Cell line selection for optimization of antibody production

• Biologicals manufacturing monitoring

Accurate antibody quantitation is critical to the selection of cell lines

for development and the optimization of bioreactor titers.

Customised protocols run on this system overcome drawbacks from

traditional measurement methods including those of lack of

specificity, labor-intensive protocols, and variable imprecision. Each

step of bioprocessing, is streamlined hence speeding the workflow

Figure: Bioreactor process time profile of

cell culture supernatants

Kinetic Characterization of Proteins

Kinetic analysis of antibodies and other proteins is critical to both

the selection of clones for development and comprehensive kinetic

characterization throughout development and production. Using

Octet Red we provide a unique approach in label-free, real-time

kinetic analysis streamlining assay development. As a result ka, k

d,

and KD.

Kinetic Characterization Data

Figure: Kinetic characterization of an antibody to a series of antigen.

Globally fitted curves and residuals



PBI006 Biomolecular interaction assay for Kinetic

characterization of proteins



PBI005 Biomolecular interaction assay for Protein Quantitation

in Bioprocessing





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Detection of Anti-Drug Antibodies (ADA)

Immunogenicity is the degree of the immune response to a

therapeutic drug. Anti-drug antibodies (ADA) produced in the

process can elicit allergic or anaphylactic reactions, reduction in

efficacy, and/or induction of autoimmunity. Currently a prerequisite

for the approval of a therapeutic protein involves strict regulatory

immunogenicity testing of biopharmaceuticals during clinical trials.

Using this technology platform we offer the pharmaceutical industry

accelerated detection of anti-drug antibodies (ADA) and

characterization of an anti-drug antibody response.

Screen for Drug Inhibitors

Using the biosensor surface within this bio-layer-interferometry

technology platform, it is possible to conduct ligand inhibition assays

by expanding the variables that can be measured, such as blocking

or unblocking analytes, affinity, and association and dissociation

rates. Hence screening of large number of drug inhibitors can be

carried out resulting in the creation of a drug blocking profile.



PBI007 Biomolecular interaction assay for Detection

of anti-drug antibodies (ADA)




PBI008 Biomolecular interaction assay for Screen

for drug inhibitors




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Monoclonal Antibody Production

iLife provides our customers a very comprehensive expertise in

the area of Molecular Biology and Immunology giving end-to-end

solutions for Recombinant Protein and Custom Antibody

development.

Monoclonal Antibody (Hybridoma) Development in

Mouse• Against a customer-supplied protein or peptide

• Delivery of best-reacting clones and/or continuous supply of

Purified Antibody

• We can also design and synthesize the peptide (immunogen)

The service package for monoclonal Ab production

can be inclusive of :• Pre-Screening of Mice against customer supplied/synthesized

Immunogen

• Antigen conjugation with carrier protein

• Primary immunization

• Affinity maturation

• Fusion of splenocytes with myolema cells

• Colony selection and screening on ELISA

• Final selection

• Single-Cell Cloning

• Mass Culture and Antibody Purification

• Quality Analysis of Purified Antibody with Western Blotting

Polyclonal Antibody Production

We provide a flexible approach for polyclonal antibody development

against protein/peptide of choice. The basic service package

comprises of antisera development which can be extended to

purification trial and final purification which is briefed as follows:

Antisera Development

• Pre-screening of animals (New Zealand White) against customer-

supplied/synthesized immunogen and selection of animals for

immunization

• Conjugation of immunogen to carrier proteins if immunogen is

less than 10 KDa.

• Primary Immunization of animals

• Secondary immunizations (boosters) and titer checks (maximum

of 4 rounds)

• Production bleed from 2nd booster onwards after satisfactory

titer (see notes below) is achieved

• Termination and exsanguination bleed after 3rd or 4th booster

(depending on titer value)

Trial Purification

• Protein A purification of Antisera or

• Antigen-Affinity purification of Antisera

• Quality analysis of purified antibody samples by Affinity check

with Antigen-Dilution ELISA & Specificity check with Western

Blot (for proteins or large peptides only)

Final Purification

• Column preparation and Purification

• Dialysis and concentration of antibody

• Quality analysis of purified antibody

• Affinity check: Antigen-Dilution ELISA

• Specificity check: Western Blot (for proteins or large

peptides only)

Antibody Production



PAD001 Monoclonal Antibody production

PAD002 Polyclonal Antibody Production



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HPLC & LC- MS Services

FOOD & AGRICULTUREFood and agro based products can be assessed for nutritional

labeling, food safety evaluations, trace analysis and shelf life studies.

Products tested :

• Fruits & vegetables (afresh, concentrates, even processed)

• Beverages

• Milk & milk products

• Water

• Spices

• Cereals & Pulses

• Baked products

• Meat & meat products

• Egg & egg powder

Expertise :

• Nutrient profiling

• Amino acid profiling

• Water soluble vitamins by HPLC

• Fat soluble vitamins by HPLC

• Food additives

• Acidulants

• Antioxidants

• Preservatives

• Artificial Sweeteners

• Synthetic colors

• Nutritional labeling

• Trace analysis

• Pesticide residues

• Antibiotic residues

• Dioxins

• Toxic metals

• Mycotoxins

• Epoxy resins

• Chemotherapeutics and antiparasitic agents

• Microbiological evaluations

• Shelf life studies

DRUG & PHARMAMethod Development and Validation

We offer analytical method development and validation services

for batch to batch uniformity, identification & quantification of

impurities or Active Pharmaceutical Ingredient (API) in formulations

and other departments involved in day to day QA/QC or R&D works

in the biopharma industry.

Method development is undertaken for:

• Assay

• Dissolution

• Purity and concentration

• Trace metals

• Residual solvents

• Physico-chemical tests

• Structural confirmation

• Metabolite monitoring

• Half life in the body

Metabolite Analysis

Custom Peptide Synthesis

Our peptide synthesis service uses both Solid Phase and Solution

Phase technologies under cGMP conditions. The high quality

peptide synthesis service guarantees to meet or exceed customer

specifications with following peptide features:

• Peptide length: upto 120 AA

• Peptide Purity: From Crude to 99%

• Quantities: from 1mg to Kilograms

• Delivery: ~3 weeks

• Sulphated/Phosphorylated Peptides

• Multiple Disulfide bridges

• Chromogenic/ Fuorogenic Substrates

• Special Amino acids & derivatives

• Peptide protein Conjugation

• Peptide modifications with C-terminal Biotin, AMC, Aldehyde, CMK

All the peptides will be delivered with a Certificate of Analysis

specifying the HPLC gradient used to determine peptide purity.

We specialize in supplying 20 to 1000 peptides with lengths up to

15 residues and quantities up to 30 mg. We also offer ~1mg of

peptide on 96 well plates. For large peptide synthesis projects, we

also offer peptide pooling.

Peptide Synthesis



PAD003 Custom Peptide synthesis



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WATER Analysis

iLife the quality of water is assessed for various applications as

mentioned below:

• Biomedical Grade water

• Electronics Grade water

• Water for Food processing Industry

• Reagent water

• Water for storage batteries

• Water for fermentation industry

• Water for Ice manufacture

• Water for Tanning Industry

• Brine solutions for trace metals.

• Feed water used for animals in safety data generation

• Cooling tower water for physico-chemical tests and Legionella.

• Trace metal analysis.



PHP001 Analytical HPLC

PHP002 Semi-preperative HPLC

PHP003 Perparative HPLC

PHP004 Customized LC-MS/MS solutions

PHP005 Customized GC-MS

PHP006 Customized GC-MS/MS

PHP007 Customized GC-MS/TOF

PHP008 Customized ICP MS


Metabolite Analysis

MALDI ToF/ToF Services

iLife is equipped with the next-generation tandem time-of-flight MS/

MS system - ABI4800 MALDI ToF/ToF Analyzer.

Peptide Mass Fingerprint (PMF) –

• Trypsinization of Protein sample

• Protein Mass Fingerprinting using MALDI ToF

• Protein identification using MASCOT protein database.

MS/MS peptide fragmentation –

• Automated spot Picker is used for the precise excision of protein

of interest for further proteomic analysis.

• Trypsinization of Protein sample

• Protein Mass Fingerprinting using MALDI ToF

• Protein identification using MASCOT protein database.

Mass Analysis- by MALDI ToF for proteins/peptides

de novo sequencing of proteins/ peptides



PMD009 Trypsinization

PMD010 Mass Analysis of Proteins

PMD011 Mass Analysis of Peptides

PMD012 Peptide Mass Fingerprinting through MALDI ToF

PMD013 MALDI ToF/ToF

PMD014 De novo sequencing by MS/MS



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Adenovirus Services

In order to address the complex issue of protein expression in variety

of cell lines and biological systems, Our team of scientists have

developed capabilities for Recombinant adenoviruses based

epigenetic gene transfer technology and corresponding protein

expression.

We offer complete services ranging from design of different

adenoviral expression platforms. Our custom services include the

overall evaluation of project, analysis of the primary and/or

transformed cell lines for which the expression systems are created

etc. We internally design appropriate primers for amplifying the

ORF (Open Reading Frame), construction of wild type ORF with

N- or C- terminal tags for sub-cellular localization and/or purification,

creation of library of mutant proteins etc prior to their integration

into the appropriate adenoviral backbone.

Small or big ORFs: Expression in hard to transfect cells lines;

Expression for large scale purification of proteins; Expression for

studying sub-cellular localization, adenovirus are the most accepted

ways for expression recombinant proteins with highest efficiency

with least undesirable effects.

Our adenoviral expression services are performed on a customized

project format.

Generation of Stably Expressing Cell Lines

We offer advanced level of research support services in generating

cell lines from different mammalian and non mammalian sources

for the research use. The cell lines are generally created with

integration of plasmid DNA of choice to create cell lines expressing

recombinant clones.

Our service is an integrated model where our team of cell biologists

works actively in coordination with the team of bioinformatics

experts, molecular biologists and proteomics specialists. We take

up stable cell line construction at its entirety where we design,

amplify clone and express the gene of interest in the first phase.

Special care taken to select the vectors for expression of the gene

of interest. We have a large repository of commercially available

and custom tailored vectors for integrating plasmid DNA constructs

encoding dual antibiotics resistance gene to ensure highly specific

clonal selection.

Our services include

1. Design of the entire project for stable cell construction

2. cDNA synthesis to expression of the protein in a mammalian

cell compatible vector with dual antibiotic resistance genes

3. Selection of the right cell line

4. Initial transient transfection study for the verification

5. Stable cell line transfection under CLASS-10000 clean room

conditions

6. Selection of multiple clones from the transfected population

7. Differential gene expression studies of the stable cell lines to

study the effect of over expression of gene of interest.

8. Constitutively active expression based recombinant cell lines

9. Stable cell lines with integrated inducible expression systems

for the study of toxic or pro-apoptotic genes.

We offer Continuous supply of viral particle preparations which are

ready to infect the cells of your interest once the viral particle

construction is completed, there by eliminating the need to set up

infrastructures in your laboratory.

Customized Cell Biology Services



PCB001 Adenovirus Services

PCB002 Generation of Stably Expressing Cell Lines

PCB003 Novel Cell Lines Development

PCB004 Other Customized Cell Biology Services


Novel Cell Lines Development

As an innovative new addition to our

capabilities, our cell biology team can

develop novel cell lines from a variety

of mammalian and non mammalian

sources. We are currently developing

cell lines of pharmaceutical relevance.

These cell lines are derived from

Indian population with different

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BioinformaticsSimplify Complex Life Research



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Introduction and ApplicationsBioinformatics or computational biology is the use of mathematical

and informational techniques, including statistics, to solve biological

problems, usually by creating or using computer programs,

mathematical models or both. One of the main areas of

bioinformatics is the data mining and analysis of the data gathered

by the various genome projects. Other areas are sequence

alignment, protein structure prediction, systems biology, protein-

protein interactions and virtual evolution.

GenomicsGenomics is any attempt to analyze or compare the entire genetic

complement of a species. It is, of course possible to compare

genomes by comparing more-or-less representative subsets of

genes within genomes.

Comparative genomicsThe study of human genetics by comparisons with model organisms

such as mice, the fruit fly, and the bacterium E. coli.

Structural genomics or structural

bioinformaticsThis refers to the analysis of macromolecular structure particularly

proteins, using computational tools and theoretical frameworks.

One of the goals of structural genomics is the extension of idea of

genomics, to obtain accurate three-dimensional structural models

for all known protein families, protein domains or protein folds.

Structural alignment is a tool of structural genomics.

Functional genomicsFunctional genomics is a field of molecular biology that is attempting

to make use of the vast wealth of data produced by genome

sequencing projects to describe genome function. Functional

genomics uses high-throuput techniques like DNA microarrays,

proteomics, metabolomics and mutation analysis to describe the

function and interactions of genes.

ProteomicsProteomics is the study of proteins - their location, structure and

function. It is the identification, characterization and quantification

of all proteins involved in a particular pathway, organelle, cell, tissue,

organ or organism that can be studied in concert to provide accurate

and comprehensive data about that system.

Proteomics is the study of the function of all expressed proteins.

The study of the proteome, called proteomics, now evokes not

only all the proteins in any given cell, but also the set of all protein

isoforms and modifications, the interactions between them, the

structural description of proteins and their higher-order complexes,

and for that matter almost everything ‘post-genomic’.

PharmacogenomicsPharmacogenomics is the application of genomic approaches and

technologies to the identification of drug targets. In Short,

pharmacogenomics is using genetic information to predict whether

a drug will help make a patient well or sick. It Studies how genes

influence the response of humans to drugs, from the population to

the molecular level.

PharmacogeneticsPharmacogenetics is the study of how the actions of and reactions

to drugs vary with the patient’s genes. All individuals respond

differently to drug treatments; some positively, others with little

obvious change in their conditions and yet others with side effects

or allergic reactions. Much of this variation is known to have a genetic

basis. Pharmacogenetics is a subset of pharmacogenomics which

uses genomic/bioinformatic methods to identify genomic correlates,

for example SNPs (Single Nucleotide Polymorphisms),

characteristic of particular patient response profiles and use those

markers to inform the administration and development of therapies.

Strikingly such approaches have been used to “resurrect” drugs

thought previously to be ineffective, but subsequently found to work

with in subset of patients or in optimizing the doses of chemotherapy

for particular patients.

CheminformaticsThe mixing of those information resources to transform data into

information and information into knowledge for the intended purpose

of making better decisions faster in the arena of drug lead

identification and optimization. Related terms of Cheminformatics

are chemi-informatics, chemometrics, computational chemistry,

chemical informatics, chemical information management/science,

and Cheminformatics.

Biomedical Informatics / Medical InformaticsBiomedical Informatics is an emerging discipline that has been

defined as the study, invention, and implementation of structures

and algorithms to improve communication, understanding and

management of medical information.

Bioinformatics Expertise

Computational ChemistryComputational chemistry is the branch of theoretical chemistry

whose major goals are to create efficient computer programs that

calculate the properties of molecules (such as total energy, dipole

moment, vibrational frequencies) and to apply these programs to

concrete chemical objects. It is also sometimes used to cover the

areas of overlap between computer science and chemistry.



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In silico Drug DesigningDrug discovery is a complex and risky treasure hunt to find the

most efficacious molecule which do not have toxic effects but at

the same time have desired pharmacokinetic profile. The hunt starts

when the researchers look for the binding affinity of the molecule

to its target. Huge amount of research requires to be done to come

out with a molecule which has the reliable binding profile. Once the

molecules have been identified, as per the traditional methodologies,

the molecule is further subjected to optimization with the aim of

improving efficacy. The molecules which show better binding are

then evaluated for its toxicity and pharmacokinetic profiles. It is at

this stage that most of the candidates fail in the race to become a

successful drug. Bioinformatics make it easier to design successful

drug with the help of bioinformatics tools and also saves time and

money.

Agroinformatics / Agricultural InformaticsAgroinformatics concentrates on the aspects of bioinformatics

dealing with study of plant genomes. Microarray techniques to study

plant disease and causing factor for it due to which genes are

suppressed or over expressed. Comparative genome analysis of

different species of plants.

Systems BiologySystems biology is the coordinated study of biological systems by

investigating the components of cellular networks and their

interactions, by applying experimental high-throughput and whole-

genome techniques, and integrating computational methods with

experimental efforts.

Bioinformatics Expertise



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Microarray data sets are very large and complex. Statistical challenges include taking into account effects of background noise and

appropriate normalization of the data. Normalization methods are different for different platforms.

Data ProcessingThis include background subtraction (global or local background), determination of spot intensities and intensity ratios, visualization of

data in different plots and log-transformation of ratios, global or local normalization of signal intensity values.

Statistical Analysis for Significant ChangesThis include paired t-test, unpaired t-test, -Test against 0, Welch t-test, Unpaired Mann-Whitney Test, The Paired Mann-Whitney Test,

One-Way ANOVA, two-way ANOVA, Multi-Way ANOVA, Friedman Test, Kruskal-Wallis Test, multiple testing correction, Clustering

(Hierarchical, K-Means, SOM). These methods assess statistical power based on the variation present in the data and the number of

experimental replicates, and can help in minimizing errors in the analysis.

Biological Contextualization of DataThis includes GO analysis, GSEA, Class Prediction, Pathway analysis etc.

Microarray Data Analysis



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Single Color and Two Color Gene Expression Data Analysis

A gene expression microarray is a technology used in genomics to

know the change in expression of gene. microarray has thousands

of spots of DNA oligonucleotides, that is called features. Each

feature contains several probes of a specific DNA sequence of

particular gene. Probes hybridize to cDNA or cRNA sample and is

detected and quantified to determine relative abundance of cDNA

or cRNA in the sample. One micro array chip contains thousands

of probes, a microarray experiment can perform many genetic tests

in parallel. This is why microarray is the first choice of any genetic

research.

Due to biological complexity of gene expression, data analysis plays

major role to find out differentially expressed genes. To begin with

data analysis, experimental design is very critical and important if

statistically and biologically significant conclusions are to be drawn

from the data. Hence at least two biological or technical replicates

are important for analysis.

Under this section we can analyze any platform based gene

expression data analysis such as Agilent single color and two color,

Affymetrix single color, Illumina single color and two color,

Nimblegen single color, GE single color, combimetrix single color

and all kinds of custom array for single color and two color.

Agilent Single Color or Two Color Microarray

Data AnalysisSend us only .txt file which has been extracted from FE software,

mention the technology name you worked with. We would do whole

data analysis and give you differentially expressed genes and

pathway associated with that.

Affymetrix Microarray Data AnalysisProvide us .CEL or .CHP file and relax. We would analyze your

experimental data and would give you valuable biological

significance of your data.

Illumina Microarray Data AnalysisSend us .txt file which has been extracted from bead studio. We

would analyse your experimental data and would give your result

which would be very much biological significant.

Different File FormatIf your experimental data is in another file format such as .xls, tab

delimited etc and is processed then also you could send us your

data for further analysis but in this case please write in detail that

how did you process your data and what algorithms you are

interested in? we only need intensity value and any gene identifier

for data analysis. We would analyze your experimental data with

your given specification and would provide you significant result.

Other Microarray PlatformWe could analyze any microarray data which might have come

from any microarray platform like GE, Nimblegen, Combimetrix,

Custom designed array etc. Please contact us and ask what you

could get from your experimental data after analysis.

Basic Analysis• Data processing

• Normalization

• Experiment Grouping

• Quality control on samples

• Quality control on probes

• Statistical Analysis

• Differentially expressed significant genes

• Fold change analysis

• Clustering of genes

• PCA Analysis

Advance Analysis• GO Analysis

• GSEA

• Class prediction

• Pathway Analysis



BOC001 One Color microarray Basic Data Analysis

BOC002 One Color Microarray Advance Data nalysis

BTC003 Two Color Microarray Basic Data Analysis

BTC004 Two Color Microarray Advance Data Analysis





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Exon Expression and Exon Splicing Data Analysis


Alternative splicing is defined as variations in RNA splicing

mechanisms resulting in multiple splice variants. Affymetrix Exon

array is used for studying the alternative splicing of genes which is

important in identifying the antagonistic functions, certain genetic

mutations which result in human diseases and understanding the

disease mechanism or biological process.

Multiple probes per exon enable “exon-level” analysis and allow us

to distinguish between different isoforms of a gene. This exon-level

analysis on a whole-genome scale opens the door to detecting

specific alterations in exon usage that may play a central role in

disease mechanism and etiology.

The second level is “gene-level” expression analysis, in which

multiple probes on different exons are summarized into an

expression value of all transcripts from the same gene.

Exon arrays provide the most comprehensive coverage of the

genome, including empirically supported and predicted transcribed

sequences, enabling the discovery of previously unidentified novel

events.


• Normalization




• Statistical analysis


• Filter Transcripts on DABG

• Splicing ANOVA

• Filter on Splicing Index

• Splicing Visualizations

• Profile Plot

• Variance Plot



• PCA analysis

Advance Analysis

• GO analysis

• GSEA


• Pathway analysis



BEE001 Exon Expression Basic Data Analysis

BEE002 Exon Expression Advance Data Analysis

BES003 Exon Splicing Basic Data Analysis

BES004 Exon Splicing Advance Data Analysis




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Copy number analysis is important to understand or to explore the

effect of copy number variation in human disease and point

mutation. Ideally there would be two copies of gene in human

genome but practically we see that there is several copy of same

gene present in genome which trigger disease or prevent disease

in human. Several thousands of SNPs can be estimated for the

copy number variation and genomic position.

This analysis includes Copy Number Variants , Copy Number

Polymorphism, Log Ratio, Loss in Heterozygosity (LOH),

cytogenetic analysis, genotyping calls, copy number calls for CNV

regions and individual probe sets, cluster graphs, Reference analysis

against control, Allele Specific Copy Number, Parent specific Copy

Number, Genomic Identification of Significant Targets in Cancer

(GISTIC) etc can be analyzed.

Copy number analysis• Quality control on samples

• Batch Effect Correction

• Statistical t-test

• PCA

• Mean log ratio

• Filtering

• GISTIC

• Clustering

• GO analysis


Copy Number Analysis



BCN001 Copy number Analysis

BAA002 Association Data Analysis


Association Data Analysis

Genome wide association study (GWAS) is extremely significant

method to identify susceptible genes involved in common human

diseases and increases chance to find targets for therapeutic

intervention that act on the root cause of disease.

This analysis requires thousands of case control sample to find

statistically significant loci which occurs due to difference in allele

or genotype frequency in case with respect to control. The difference

in allele or genotype frequency can lead to disease and thus this

method is useful in disease gene discovery due to unbiased study

of gene with known or suspected disease mechanism.

Analysis includes• Quality control on samples

• Filter to get significant SNP

• PCA


• Pearson’s chi square test

• Fisher’s exact test

• Cochran-Armitage test

• Regression analysis

• Linkage Disequilibrium (LD) analysis



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miRNA Data Analysis

miRNA is small single-stranded, regulatory RNA molecules of

approximately 25 nuceotide base long molecule. miRNA regulates

mRNA by binding with complementary sequences of mRNA and

affects the expression of genes involved in several biological

processes. Therefore miRNA expression study became important

class to understand the role of miRNA in regulating biological

processes. miRNA array developed to study expression patterns

of miRNA for any conditions and can be correlated with their target

gene expression studies.


• Normalization








• PCA analysis

Advance Analysis• Find target gene (Target Scan)

• GO analysis

• GSEA






BMR001 miRNA microarray Basic Data Analysis

BMR002 miRNA microarray Advance Data Analysis


Custom Chip Design and Analysis

To study gene expression at genomic scale mostly microarray chips

are, it is important to design oligo probes that specifically represent

large number of DNA sequences. Cross-hybridization is most likely

from the regions with contiguously and perfectly matched bases.

Oligo probes should also be sensitive and hybridize under similar

conditions. We design chip for diagnostic purposes by picking

specific oligos by skipping regions with contiguous bases common

in other sequences. We assure for specificity and it is double-

checked by NCBI BLAST. Similar sequence regions to non-coding

RNAs are avoided because total RNA are often used for array

hybridization. Low-complexity regions are also filtered out to

maintain oligo specificity. Oligos and sequence regions that may

form secondary structures are discarded since both the probes and

the sequence target sites should be easily accessible for

hybridization.

Parameters are taken in consideration while primer

designing:Probe Copy, Probe Length, Tm range, Cross-reactivity, BLAST

score, End preference, Filter file, Cross-reactivity to filter sequences,

Cross-reacting probes.

We provide services for custom chip design for microarray

experiemnt. Custom chip design could be done on user’s

preferences. Selected probes or genes of user’s interest can be

spotted on glass slide to carry out higher level of research.

This is also like another microarray chip but only difference would

be in quantity and specificity of genes, for certain experiment.

Analysis of data would be same as per other microarray experiment.

Basic Analysis• Normalization





• Differentially expressed

significant genes



• PCA analysis

Advance Analysis• GO analysis

• GSEA





BOP001 Other platform Basic Data Analysis

BOP002 Other platform Advance Data Analysis

BCC003 Chip-on-chip Data Analysis

BDM004 DNA Methylation Data Analysis

BCD005 Custom Chip Design




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Real Time PCR is used to measure the quantity of DNA, cDNA, or

RNA present in a sample. It is extremely sensitive technique for

mRNA detection and quantization. RT-PCR can be used to quantify

mRNA levels from very smaller samples. Therefore, this technique

is very sensitive to enable quantitation of RNA from a single cell. A

DNA-binding dye binds to all double-stranded DNA in PCR that

causes fluorescence. An increase in DNA product during PCR leads

to an increase in fluorescence intensity and is measured at each

cycle, this allows DNA concentrations to be quantified. However,

DNA dyes bind to all DNA PCR products, including nonspecific

PCR products like Primer, dimer. This can potentially interfere with

or prevent accurate quantification of the intended target sequence.


• Normalization








• PCA analysis

Advance Analysis• GO analysis

• GSEA



RT PCR Data Analysis



BRT001 RT PCR Basic Data Analysis

BRT002 RT PCR Advance Data Analysis




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The comparative genome analysis is done to establish the

correspondence between genes or other genomic features in

different organisms. We can create intergenomic maps that make

it possible to trace the evolutionary processes responsible for the

divergence of two genomes. At the lowest level, point mutations

affect individual nucleotides. At a higher level, large chromosomal

segments undergo duplication, lateral transfer, inversion,

transposition, deletion and insertion. Many of these studies are

based on the homology detection and protein families computation.

Biological Systems ModelingThis involves the use of computer simulations of the networks of

metabolites and enzymes which comprise metabolism, signal

transduction pathways and gene regulatory networks to analyze

and visualize the complex connections of these cellular processes.

Sequence Analysis• Determination genes that encode proteins, RNA, regulatory

sequences, structural motifs, and repetitive sequences.

• Intra species and inter species gene comparison to know

similarities between protein functions, or relations between

species.

• High throughput genome sequence analysis for inter-related,

non-identical overlapping sequences of closely related varities

• Whole Genome reconstruction.

Evolutionary Studies• Tracing the evolution of an organism based on the changes in

its DNA contents.

• Construction of complex evolutionary computational models.

Gene Annotation• Target Gene finding to search for protein-coding genes and other

functional sequences within a genome.

Comparative Genomics



BGW001 Genomics Work in Bioinformatics




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Comparative Genomics

Primer Designing

PCR is a common technology applied in the biomedical and

biotechnology field. This technology is used for fast mass duplication

of DNA sequences. By repeating some cycles of the denaturation,

annealing and extension reactions, it allows a small amount of DNA

to be amplified exponentially. Typically 25–45 of these cycles are

performed. Amplification of specific regions of the genome is

determined by specific primer sets with forward and reverse

orientation. Typically considered primer design constraints are the

length of the primer, the GC content, the melting temperature and

GC clamp. The length of primers should be within 16 bps~28 bps,

while the difference of the primer pair length should not exceed 3

bps. The GC content of primers should be within 40%~60%. The

melting temperature of primers should be within the range of

50oC~62

oC, while the difference of the melting temperature of a

primer pair should not exceed more than 5oC. Finally, the 3’ end of

primers should be G or C.In recent years, secondary structures

were applied to primer design, such as dimer, hairpin and specificity

of a primer pair. Use dynamic programming to design primers. This

allows for designing multiple primers for multiple target DNA

sequences. We identify the specificity-determining subsequence

of each primer and examine its uniqueness in the target genome.



BPD001 Primer Designing

BPW002 Proteomics Work in Bioinformatics




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Peptide Library Synthesis

We could generate peptide of your interest from the given protein.

We could help you in peptide array design to study the specific

protein of your interest. Peptide library is a systematic combination

of different peptides in large number. It is widely used in :

• Drug screening

• Target validation

• Epitope mapping

• Vaccine development

• Peptide biological assay

• Protein-peptide binding assay

• Immune monitoring

• Structure-activity studies.



BPL001 Peptide Library Synthesis


Protein is made up of amino acids and the information for protein

synthesis (primary structure) during translation comes from codon.

Codon is the three nucleotide sequence on gene that is why we

could determine primary structure of protein from the gene of any

species. It is vital to know the structure of protein (secondary, tertiary

and quaternary) which may cause disease or prevent the disease.

Bioinformatics is used for the study of homology modeling to predict

the function of a gene with unknown function. if a gene X with

known function is homologous to the sequence of gene Y whose

function is not known, but we could conclude that while gene X and

Gene Y share same genetic information then function of gene Y

could be the same as function of gene X. In the structural

bioinformatics, homology is used to determine which parts of a

protein are important in structure formation and interaction with

other proteins and this technique is called homology modeling.

With the help of bioinformatics tool we could predict protein-protein

interaction to identify and catalog physical interactions between

pairs or groups of proteins. Protein–protein interactions is important

for the investigation of intracellular signaling pathways, modeling

of protein complex structures and for gaining insights into various

biochemical processes.

• Prediction of co-evolved protein pairs based on similar

phylogenetic trees

• Identification of homologous interacting pairs

• Identification of structural patterns

• Network visualization of protein–protein interactions



BPA001 Peptide Array Design

BPP002 Protein-Protein Interaction


Protein-Protein Interaction Studies

Prediction of Protein Structure



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The advanced step of microarray technique wherein researchers

concentrate on small number of proteins and their role in biological

system. Antibody microarray technique is used to discover and

validate protein biomarkers in different samples. This is also used

as screening techinique for biomarker discovery and rapid

expression profiling of proteins.

Antibody microarray experiment is inexpensive for throughput

analysis of proteomic profile which allows researchers to focus on

a small number of proteins that have biological relevance.

Antibody microarray data analysis includes :• Data processing

• Dye swapping

• Global Normalization

• Local Normalization

• Quality control on entities


• Filter on entities



• Clustering analysis

• PCA analysis

• Data visualization in different plot

• Scatter plot

• Profile plot

• Box whisker plot

• Histogram

• MvA plot

• PCA plot



BAM001 Data Analysis for antibody microarray 500

BAM002 Data Analysis for pathway focused antibody

microarray


Antibody Microarray Data Analysis



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For Protein Applications

Sample Type Sample Shipment Guidelines

Tissue Transport it in Frozen Condition i.e. Dry Ice

Cell lysate Lyophilized Form(Preferred) or ship it in Frozen condition (Dry Ice)

Culture Supernatant Lyophilized Form(Preferred) or ship it in Frozen condition (Dry Ice)

Blood Plasma Transport it in Frozen Condition i.e. Dry Ice

Body Fluids Lyophilized Form(Preferred) or ship it in Frozen condition (Dry Ice)

For DNA Applications


DNA In Dry ice/Liquid nitrogen

Animal Tissue Suspend in Ethanol & transport it in dry ice/liquid nitrogen

Plant Tissue Suspend in Ethanol & transport it in dry ice/liquid nitrogen

Cells Peletted and freezed & transport it in dry ice/liquid nitrogen

Blood Collect in heparin coated vaccutainers & transport it at 4 degrees (gel packs)

For RNA Applications


RNA Precipitate in sodium acetate & transport it in dry ice/liquid nitrogen

Animal Tissue Suspend in RNA Later & transport it in dry ice/liquid nitrogen

OR

Directly freeze the tissue & send it in dry ice/liquid nitrogen

Plant Tissue Crushed and suspended in RNA Later & send it in dry ice/liquid nitrogen

OR

Directly freeze the tissue & send it in dry ice/liquid nitrogen

Cells Peletted, suspended in RNA Later & transport it in dry ice or liquid nitrogen

Blood Collect in heparin coated vaccutainers & transport it at 4 degrees (gel packs)

Shipment Guidelines



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U.S., CANADA & EUROPE ORDERING

By Mail: Imperial Life Sciences USA, Inc.

1630-1634, Centre Pointe Drive, Milipitas

CA, 95035- USA

By Telephone: 9:30 AM to 7:00 PM IST

+1 408- 907 6645

By Email: [email protected]

By Internet: http://www.ilifediscoveries.com

Currency: US Dollars ($)

ASIA ORDERING

INDIA

By Mail: iLife Discoveries

Plot No. 154 S, Sector- 7

IMT Manesar, Gurgaon

Haryana- 122 050

By Telephone: 9:30 AM to 7:00 PM IST

+91 124- 4779800

By Fax: +91 124 4779801


By Internet: http://www.ilifediscoveries.com

Currency: Indian Rupees (INR)

NOTE: All Orders are to be placed in the name of

iLife Discoveries P. Ltd.

iLife will not be responsible for any abberations in the

purchase orders.

TECHNICAL SUPPORT

By Telephone: +91 124- 4779800


By Fax: +91 124- 4779801

PRICES

• Please contact our Customer Support Team

([email protected]) to obtain a quote for

the desired published services.

• Please provide the Service no. and Quantity of the samples for

desired services, when contacting our Customer Support Team.

• Customized quotes can be provided based on various factors

such as nature & duration of the project. Please contact our

Customer Support Team ([email protected])

for customized quotes.

NOTE: All customized projects need additional background

research by pour scientific team which may result into an

additional time to submit a quotation.

• All accounts with Annual Rate Contract with iLife Discoveries

to be exercised with Price list under Rate Contract. Please

contact for more details.

SHIPPING

• An additional handling charge of INR1000.00 per order is

applicable.

• An additional charge of INR 1200.00 per shipment is applicable

for shipments requiring Dry Ice.

• Customized sample collection, shipment & handling at customer

site, anywhere in India are offered at an additional cost. Please

contact our Customer Support team for details.

PAYMENT

• 100% Advance payment is required along with the Purchase

Order for those customers with no accounts established with

iLife. Please contact our Customer Support team

([email protected]).

• As outcome of scientific experiments depend upon several

factors such as sample source/Quality, shipping, sample

storage at customer site, iLife Discoveries does not guarantee

the expected outcome in all cases of the experiment. Payment

should be made irrespective of data generated.

• The supply of analysis report and/or products ordered to iLife

shall be made within stipulated time mentioned in the Quotation

or order acceptance or rate Contract agreement letter.

• As and where, iLife operates through an Authorized Channel

Partner, the supplies and billing will be routed directly by iLife

Discoveries and/or by the Authorized Channel Partner.

FOR GOVERNMENT CUSTOMERS

• Please enquire with our Customer Support Team regarding iLife

Discoveries government-discounts schedule.

EXCLUSIVE TERMS FOR SALE

• iLife Discoveries does not agree to or is not bound to any other

terms & conditions, unless those terns & conditions have been

expressly agreed to in written by a duly authorized officer of iLife.

• Request for storage of samples is agreeable with an additional cost..

• Request for return of samples will not be accepted.

Terms & Conditions



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Microarray technology

Training on the Agilent microarray system includes:

Introduction to the microarray technology

Custom microarray designing

Microarray types & applications

Experimental workflow including

Gene Expression microarray experimental runs

Duration: 10 working days

High-end Technology Training

Quantitative PCR technology

Training on Quantitative PCR includes:

Introduction to the QPCR technology

Experimental workflow

Primer/ Probe designing, synthesis and validation studies


Agilent 2100 Bioanalyzer

Training on Bioanalyzer includes:

Introduction to Bioanalyzer technology

Qualitative and Quantitative Analysis of DNA

Qualitative and Quantitative Analysis of RNA(RIN number)

Qualitative and Quantitative Analysis of Protein

Troubleshooting




GHT001 Microarray Technology Training (Academic)

GHT002 Quantitative PCR Technology Training (Academic)

GHT003 Agilent 2100 Bioanalyzer Technology Training (Academic)

GHT004 Microarray Technology Training (Corporate)

GHT005 Quantitative PCR Technology Training (Corporate)

GHT006 Agilent 2100 Bioanalyzer Technology Training (Corporate)

GHT007 Customized High end Technology Training




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forteBio Octet Technology Training

Training on Octet includes

Introduction to Biolayer Interferometry

technology and Instrumentation

Protein Quantitation studies

Kinetic Characterization studies

Small molecule and small peptide studies




PHT001 ForteBio Octet Technology Trainig (Academia)

PHT002 ForteBio Octet Technology Trainig (Corporate)

PHT003 Customized Octet Technology Training (Academia)


iLife Catalog

Documents

Transcript of iLife Catalog