III. Genetics & Genetic Engineering Presentation # 3 Ch. 11, 13, & 14 Gregor Mendel - the “Father...
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Transcript of III. Genetics & Genetic Engineering Presentation # 3 Ch. 11, 13, & 14 Gregor Mendel - the “Father...
III. Genetics & Genetic EngineeringPresentation # 3 Ch. 11, 13, & 14
• Gregor Mendel - the “Father of Genetics”
• Was an Austrian monk• Worked in a monastery garden• Cross-pollinated plants, studied
traits (characteristics) of offspring• Looked at true-breeding pea
plants - if pollinated produced offspring identical to themself
• Looked at hybrids - the offspring of crosses between parents of different traits
Some of Mendel’s crosses:
Seed Shape
Flower Position
Seed CoatColor
Seed Color
Pod Color
Plant Height
PodShape
Round
Wrinkled
Round
Yellow
Green
Gray
White
Smooth
Constricted
Green
Yellow
Axial
Terminal
Tall
Short
Yellow Gray Smooth Green Axial Tall
Section 11-1
III. Genetics – A . Terms to Memorize:
• 1. Gene - units of DNA passed from parent to offspring. Each adult has two copies of each gene - 1 from each parent
• 2. Allele - a specific form or expression of a gene trait– Example - brown eyes, Curly hair.
• 3. Dominant - an allele that is always expressed or seen– Dark pigments are usually dominant
• 4. Recessive - an allele that is can be hidden, it will not be expressed if present with a dominant allele
• 5. Phenotype - actual gene expression - what is physically seen• 6. Genotype - the actual pair of alleles present
– Homozygous = same 2 alleles in gene pair BB, bb (purebred)– Heterozygous= = different alleles present in gene pair Bb, Tt (Hybrid)
• 7. Probability - the likelihood that a particular event will occur - Q:If you flip a coin 4 times in a row, what is the probability that it will land on tails every time? - A: 1/2 x 1//2 x 1/2 x 1/2 = 1/16 Each coin flip is an independent event. - Q: What is the probability of having 3 girls in a row? - A: 1/2 x 1//2 x 1/2 = 1/8 Each baby is an independent event.
III. Genetics
– B. Genetic Crosses• 1. Monohybrid - 1 trait crosses
– Studies 1 set of alleles from both parents– a) identify the trait and letters to be used - brown hair (B) or blond (b)– b) write the genotypes - i.e. Bb or BB or bb for both parents– c) separate the alleles into possible gametes b , B for each parent– d) draw a Punnett square and write one allele by each row and column– Join gametes together– Determine ratios of phenotypes and
genotypes
b
B
BB
Probability can help us predict the outcomes of genetic crosses.
III. Genetics
– B. Genetic Crosses• 2.Dihybrid - 2 trait crosses
– Studies 2 sets of alleles from both parents– Steps are the same but more complex because of more combinations– To identify # of possible gametes - look at how many different alleles there are
for each trait then multiply.– Example - BbFf - 2 different b’s and 2 different f’s 2x2=4– Use foil to get possible gametes f-firsts, 0-outer, i-inner, l-lasts– Use punnett square to determine offspring
Dihybrid Cross
bf
bF
Bf
BF
bfbFBfBF
BBFF BBFf BbFF BbFf
BBFf BBff BbFf Bbff
BbFF BbFf bbFF bbFf
BbFf Bbff bbFf bbff
An example of a Dihybrid cross
Another Dihybrid cross:Section 11-3
III. Genetics
– B. Genetic Crosses• 3. Incomplete Dominance
– Two alleles are neither dominant or recessive– The two show a blending of their phenotypes– Example: red carnations crossed with white carnations produce all pink
carnations– CRCR= red CWCW = white CRCW = pink
• 4. Co dominance – Two alleles are both dominant or expressed at the same time– Example: hair color in cattle Red and White hair crossed = Roan– HRHR = red hair HWHW = white HRHW = roan (red and white hair)
IncompleteDominance:
III. Genetics– B. Genetic Crosses
• 5. Polygenic traits – More than one gene will determine phenotype– Hair color in humans is controlled by more than one gene– Eye color in humans also is polygenic– Epistatic traits - one gene exerts control over another gene expression. - Ex: More than 12 pairs of alleles interact in various ways to produce coat color in rabbits. - Ex: 2 gene pairs interact together to produce 8 types of combs in roosters.
• 6. Multiple Alleles – More than two alleles are possible for one gene– Example: Blood type
» Type A = “A” antigen on the blood cell IA = A allele» Type B = “B” antigen on the blood cell IB = B allele» Type O = NO antigen on the blood cell i = O allele
Multiple alleles- Blood Typing
Polygene inheritance
III. Genetics– C. Genetic Disorders/Diseases
• 1. Detection - obtaining fetal cells to do karyotyping and biochemical tests– A) amniocentesis (see next slide)– B) Chorionic villus sampling (see next slide)
• 2. Sex-linked traits - genes only found on the X or Y chromosomes– A) colorblindness– B) hemophilia– C) muscular dystrophy – Do a Punnett Square example:
Y
XC
XcXC
XCXC XCXc
XCY XcY
• Question: • Can 2 normal parents havea colorblind child? If so, what is the sex of that child?• A: Yes (If mom is a carrier). Male.
III. Genetics
– C. Genetic Disorders/Diseases• 4. Gene mutations - changes in DNA sequence caused by exposure to radiation or
chemicals, crossing over or genetic errors– Sickle-celled anemia - blood cells are misshaped due conditions of low oxygen
» Recessive trait, no known cure– Cystic fibrosis - recessive allele, causes thick mucous build up in the lungs and
intestines, can cause liver disease, diabetes» Recessive trait, no know cure
– Tay-Sachs - slow degenerative disease of optic and mental function in young children
» Recessive trait carried on chromosome 15, no known cure
D. How have humans created new breeds?• Selective Breeding - allowing
only those animals with desired characteristics to produce the next generation.
• Ex: breeds of dogs, horses, cats, farm animals, crops.
• Hybridization - crossing dissimilar individuals to bring together the best of both organisms. Ex: daisies, crops
• Inbreeding - continued breeding of individuals with similar characteristics.
• Ex: Golden retrievers, German shepherds
• All of the above have been done for years, without altering the genetic code.
www.vwgs.com/
IV. DNA Technology
– A. Genetic Engineering-making changes in the DNA code• Restriction Enzymes- proteins that “cut” DNA at specific locations,
looks for a certain nucleotide (base) sequence• DNA recombination
– Cutting and splicing pieces of DNA into other strands of DNA» Plasmids - circular DNA molecules found in bacteria, separate
from other bacterial DNA» Sticky ends - matching or complimentary segments of DNA that are
produced by restriction enzymes» Human genes can be inserted into bacterial plasmids so the bacteria can
produce human enzymes or proteins = recombinant DNA
QuickTime™ and aCinepak decompressor
are needed to see this picture.
How restriction enzymes are used to edit DNA:• Enzymes cut the DNA molecule at a certain site.• Different restriction enzymes recognize and cut different sequences of
DNA.• The cut ends are called sticky ends because they may “stick” to other
complementary bases by means of H bonds.• Can then take a gene from one organism and attach it to the DNA of
another organism = Recombinant DNA.
Overview: Making aRecombinant DNA molecule.
• Ex: Genetically Engineered Insulin - Produced by splicing the human gene for making insulin into the plasmid of E.coli host cells.
• The genetically modified bacteria then produces insulin; it is collected and used for diabetics.
• Was 1st recombinant DNA drug approved for use in humans.
• Another Ex: Human Growth Hormone
Example: Steps in producing Human Growth Hormone
http://www.pbs.org/wgbh/nova/sheppard/labwave.html
DNA Fingerprinting:
Process of cutting apart DNA from two sources and comparing the results from gel electrophoresis.
Utilized in criminal investigations and paternity/maternity cases. (No individual is exactly alike.)
Weblink
Example of Using DNA Technology:
DNA plus restriction enzyme
Mixture of DNA fragments
Gel
Power source
Longer fragments
Shorter fragments
Section 13-2
Figure 13-6 Gel Electrophoresis
DNA Fingerprinting Procedure: • A small sample of DNA is cut with a restriction enzyme. (From sperm, blood, hair, or other material.)• The DNA fragments are separated by size using gel electrophoresis.• The shorter fragments move faster toward the + charge.• Patterns of bands are compared to see if suspect’s band pattern is the same as that of the
crime scene material.
Applications of Genetic Engineering:
• Transgenic Organisms - they contain genes from another species
• Examples:• tobacco plant which glows in
the dark (see top photo) • corn which produces a natural
pesticide • mice with similar immune
systems as humans - are used study effects of diseases
• sheep which carry a gene for a human blood protein. They secrete it in their milk - helps patients with cystic fibrosis. (See GM sheep, bottom photo)
www.mun.ca/.../Luciferase_ reporter_gene.htm
More applications of Genetic Engineering: Cloning. Steps of cloning:
A donor cell is taken from a sheep’s udder. Donor
Nucleus
These two cells are fused using an electric shock.
Fused Cell
The fused cell begins dividing normally.
EmbryoThe embryo is placed in the uterus of a foster mother.Foster
Mother
The embryo develops normally into a lamb—Dolly
Cloned Lamb
Egg Cell
An egg cell is taken from an adult female sheep.
The nucleus of the egg cell is removed.
Section 13-4
Decision Making - Safety and Ethical Issues of DNA Technology:
• Can DNA technology create hazardous new pathogens? Could they escape from the lab?
• Is genetically modified food safe to eat?• Can transgenic plants pass their new
genes to other plants in wild areas?• Who should be allowed to take Human
Growth Hormone?• Should we try to eliminate genetic
defects in our children?• Should everyone get a DNA fingerprint
ID?• Can the Human Genome Project result
in human health discrimination?
Chromosomal Abnormalities and Nondisjunction
• Nondisjunction in meiosis results in gametes with abnormal chromosome number
• Most cases produce gametes that are not viable
Down Syndrome – Trisomy 21
• Extra 21st chromosome
• Causes physical and mental abnormalities
Trisomy 18 Edward’sSyndrome
Trisomy 13 Patau’s
Syndrome
Turner’s Syndrome
• Female with only one X chromosome (XO)
• Sterile
Klinefelter’s Syndrome
• Male XXY, XXXY, or XXXXY• Sterile
Jacob’s Syndrome (XYY)
• Men are mostly normal• Increased aggression
and learning disabilities