II. Beta Oksidasi english.ppt
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Transcript of II. Beta Oksidasi english.ppt
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Enormous energy reserves because its stored in the reduced
form and anhydrous (very non-polar). Oxidation produces 9 kcal / g.
In mammals the main site of TAG reserved is cytoplasm adipose (fat cells). Adipose cells specially for
the synthesis, storage and mobilization of TAG into fuel molecules to be transferred
to other tissues by the blood
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First Stage TAG hydrolysis by Lipase
TAG + 3 H2O (lipase) Glycerol + FA
Lipase activity of adipose cells is regulated by several hormones
Epinephrin, norephineprin, glucagon, adrenocorticotropic hormone is to
induce lipolysis
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Digestion - Hydrolysis Reaction
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Knoop (1904): Fatty acids are broken down by release 2carbon unit in every stage
Fatty acids are broken down by beta oxidation to carbon beta
oxidationFatty acid oxidation in mitochondria
Fatty acid activation is required before entering the mitochondrial matrix
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In the outer membrane of mitochondria and is catalyzed by enzyme acyl CoA synthetase (fatty acid tiokinase)
ATP stimulate the formatio n of thioester bond formation between the carboxyl group
of sulf hidril FA with the CoA:
R-COO + ATP R-COO-AMP (adenylate acyl) + PPi
Adenylate acyl + HS-CoA R-COO-S-CoA (acyl-CoA)
PPI pirofosfatase hydrolyzed by the reaction becomesirrev to the right
R-COO + CoA + ATP R-COO-S-CoA (acyl CoA) + AMP + 2 Pi + 2H
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Initial Step: Requires an ATP to synthesize acetyl CoA with the fatty acid.
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FA activated in outside the mitochondria, whereas oxidation in the
mitochondria Long-chain acyl
CoA (active) can not transferred into the inner mitochondrial membrane
need special transport mechanism conjugated with carnitine
Carnitine: zwitter ions formed from lysine catalyst:
carnitine acyl transferase I (bound to the outer mitochondrial membrane)
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Why beta-oxidation?
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Alfa oxidation In the seeds of plants that are sprouting.
COOH group is released as CO2C-alpha-oxidised aldehyde
further oxidation by NAD + COOH group Omega oxidation
In the hearts of several animal tissuesTG acid omega oxidation of alpha, omega-
dicarboxylic Beta oxidation
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Fatty acids are the primary fuel for humans and mammals
Fatty acids catabolyzed by O2 to CO2 and H2O
40% of the free energy produced by the cells preserved for forming ATP,
the remaining energy is released as heat
Catabolism of fatty acids occurs in mitochondria through a process known as beta oxidation
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The process of fatty acid oxidation (after activated to form acyl CoA)
Takes place in mitochondria
Four reactions stages:
1.Oxidation (dehydrogenation) by FAD2. Hydration
3. Oxidation by NAD
4. Tiolisis by CoA
The four reactions stages, will shorten the two carbon atoms in the acylchains and formed FADH2, NADH and acetyl CoA
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• Contribute to the formation of double chains of atoms C2 - C3.
• Has a hydrogen acceptor FAD+.
• Between the different fatty acids in length different enzyme
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•Catalyzes the hydration of trans enoyl CoA
•The addition of a hydroxyl group at C no. 3
•Enzyme is stereospecific•Generate 3-L-
hidroksiasil Co. A
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•Catalyzes the oxidation of-OH at C no. 3 / C β into a ketone
•Electron acceptor: NAD+
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• Splitting of carbon atoms between C alpha and C beta
• β-Ketotiolase catalyze thioester bond breaking.
• Acetyl-CoA removed and the remaining fatty acid acyl co A is connected with tio cysteine via thioester bonds.
HSCoA replace cysteine thiol thiols, generating fatty acyl-CoA (whichhave been reduced by 2 C).
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ExamplesBeta oxidation of Myristic Acid
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To be continued withFinal Exam of
Fatty Acid Catabolism