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Transcript of If you have questions check out our facebook group!! Jacob Matson [email protected].
![Page 1: If you have questions check out our facebook group!! Jacob Matson matsonj@uci.edu.](https://reader035.fdocuments.us/reader035/viewer/2022070413/5697bfd31a28abf838cac47d/html5/thumbnails/1.jpg)
If you have questions check out our facebook group!!
http://www.facebook.com/groups/350201601657281/
Jacob [email protected]
![Page 2: If you have questions check out our facebook group!! Jacob Matson matsonj@uci.edu.](https://reader035.fdocuments.us/reader035/viewer/2022070413/5697bfd31a28abf838cac47d/html5/thumbnails/2.jpg)
Energetics• Enzymes stabilize transition state, lower activation energyGibbs free energy not at equilibrium:• ∆G = ∆G° + RTlnQ R=8.314 J/(K*mol) Q = [product]/[reactant]At equilibrium:∆G = ∆G° + RTlnKeq ∆G = 0, easy to solve for Keq
![Page 3: If you have questions check out our facebook group!! Jacob Matson matsonj@uci.edu.](https://reader035.fdocuments.us/reader035/viewer/2022070413/5697bfd31a28abf838cac47d/html5/thumbnails/3.jpg)
Energy Coupling• Link an unfavorable reaction (+ ∆G)with a favorable one (- ∆G) • Ex: many unfavorable reactions are coupled with ATP hydrolysis
∆H: Enthalpy: heat given off∆S: Entropy: how disordered something is
∆G = ∆H - ∆TS• A reaction has a ∆H = 0.5 kcal/mol, ∆S of 0.001 kcal/mol at 27 C• What will ∆G be? Above what temperature will the reaction be
favorable?∆G = 0.5-300*.001=.20 = .5-T*.001 T=500K
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Pyruvate dehydrogenasename product
E1 pyruvate dehydrogenase CO2
E2 dihydrolipoamide acetyltransferaseAcetyl CoAE3 dihydrolipoamide dehydrogenaseNADH
![Page 5: If you have questions check out our facebook group!! Jacob Matson matsonj@uci.edu.](https://reader035.fdocuments.us/reader035/viewer/2022070413/5697bfd31a28abf838cac47d/html5/thumbnails/5.jpg)
Prosthetic groups E1 TPP – thiamine pyrophosphate
E2 lipoic acid
E3 FAD
Note: overall reaction is Oxidative decarboxylation
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Where is each vitamin found?
B1: thiamine -> TPP
B2: riboflavin -> FAD
B3: niacin -> NAD
B5: pantothenic acid -> coenzyme A
![Page 7: If you have questions check out our facebook group!! Jacob Matson matsonj@uci.edu.](https://reader035.fdocuments.us/reader035/viewer/2022070413/5697bfd31a28abf838cac47d/html5/thumbnails/7.jpg)
Which of these does not inhibit deactivation of pyruvate dehydrogenase (E1)?
A. Ca2+ calcuim activates the phosphatase, releasing inactivation
B. NADC. CoAD. ADPE. Pyruvate
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What are the products of the TCA cycle (1 cycle)?
2CO2, 1 ATP or GTP 1QH2, 3NADH
How many ATP are made per NADH /QH2?
2.5 per NADH1.5 per QH2
![Page 9: If you have questions check out our facebook group!! Jacob Matson matsonj@uci.edu.](https://reader035.fdocuments.us/reader035/viewer/2022070413/5697bfd31a28abf838cac47d/html5/thumbnails/9.jpg)
How many ATP are made by oxidation of 1 glyceraldehyde-3-phosphate?
Glycolysis= 2 atp + 2.5 from nadh+2.5 from nadh pyruvate carboxylase+ 10 from TCA=17 ATP
![Page 10: If you have questions check out our facebook group!! Jacob Matson matsonj@uci.edu.](https://reader035.fdocuments.us/reader035/viewer/2022070413/5697bfd31a28abf838cac47d/html5/thumbnails/10.jpg)
What enzyme catalyzes formation of the following molecule?
Succinyl-coa synthetase
Succinyl-coA to succinate
Makes one GTP/ATP
![Page 11: If you have questions check out our facebook group!! Jacob Matson matsonj@uci.edu.](https://reader035.fdocuments.us/reader035/viewer/2022070413/5697bfd31a28abf838cac47d/html5/thumbnails/11.jpg)
Draw the product of the TCA enzyme linked to the electron transport chain.
Succinate to fumarateBySuccinate dehydrogenase
AkaComplex IIIn
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Which enzymes (TCA Cycle) produce NADH + CO2 through oxidative
decarboxylation?
Isocitrate dehydrogenase(isocitrate to alphaketoglutarate)
Alphaketoglutarate dehydrogenase(alphaketoglutarate to succinyl coa)
![Page 13: If you have questions check out our facebook group!! Jacob Matson matsonj@uci.edu.](https://reader035.fdocuments.us/reader035/viewer/2022070413/5697bfd31a28abf838cac47d/html5/thumbnails/13.jpg)
Draw the product of the final oxidative step of the TCA cycle.
Malate dehydrogenaseMalate to oxaloacetateMakes nadh
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Which of these does not inhibit citrate synthase?
A. ATPB. Succinyl-CoAC. NADHD. IsocitrateE. They all inhibit it
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You discover a new species with 5 electron transport chain proteins. In what order will
electrons flow through these proteins? E0’
A 0 VB 1 VC -1 VD 3 VE -2 V
Start: E C A B D end
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How many protons are pumped across each complex of the ETC?
I: 4 NADHII: 0 succinateIII: 4 IV: 2Where do NADH / Succinate drop off electrons?
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How many hydrogens need to be transported across the inner membrane to make 1 ATP?
4
3 go through atp synthase1 needed for symporter of H and Pi
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How many hydrogens are pumped by the ETC per oxidized glucose molecule?
10 hydrogens per nadh6 per qH2Glycolysis: 2nadhPyruvate dehydrogenase: 2 nadhTca: 6 nadh 2 QH2Total 10 nadh 2 qh2 100 + 12 = 112 hydrogens
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FILL OUT YOUR EVALS PLEASE
GOOD LUCK WITH THE FINAL!!!!!!
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Tired of studying? Watch these videos!!
Glycolysis regulationhttp://www.youtube.com/watch?v=LHMLHCx0ASM&t=2m20sHey There Pyruvatehttp://www.youtube.com/watch?v=6TOdcaNw3IAMaking ATPhttp://www.youtube.com/watch?v=YyN0wx2AHfE&feature=relatedOxidate it or Love ithttp://www.youtube.com/watch?v=VCpNk92uswY
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If you want them, my answers will also be posted online at the link below within a day after this review. I recommend you try each problem first before seeing the answer to test how much you know.
https://webfiles.uci.edu/matsonj/Winter2012/98reviewanswers.ppt