Identification of possible biological stains for differential staining of Normal and Malignant...
-
date post
21-Dec-2015 -
Category
Documents
-
view
220 -
download
4
Transcript of Identification of possible biological stains for differential staining of Normal and Malignant...
Identification of possible biological stains for differential
staining of Normal and Malignant tumour tissue
Nor Hayati Othman,
Pathology dept, PPSP
Objectives
• To search for suitable natural stains derived from national plants of Malaysia
• To determine the differential staining of the new stain on human normal and malignant tumour tissue
Normal Vs cancer cell
Normal celln:c 1:4-8smooth nuclear membraneno nucleus
Malignant celln:cmore hyperchromatic [more basic]nuclear membrane coarsechromatic coarse, stippledmitosispleomorphic
Cytoplasm - ± opaque
Those are easy cases!!
• Subtle changes in cell require further tests
• Immunohistochemsitry– We stain the proteins found in cells eg muco-
proteins, keratins, vimentins, osteobilins, neurofilaments etc
– Every protein has different characteristics and could be stained
The stain should be
• Permanent
• Able to differentiate acidic nucleus from basic cytoplasm
• Able to pick up loss of desmosomes when normal epithelial cell become malignant
• Able to detect changes in nucleus when cells become malignant
How to verify the stains?
• 100 Normal and 100 Epithelial Malignant human tissues obtained from the archives of Pathology laboratory.
• The ability to differentiate will be scored and statistically analysed