ISOLATION AND CULTIVATION OF VIRUSES

Cultivation and identification of viruses

What are viruses?

Viruses are infectious ,intracellular,obligate,parasites

Methods to cultivate animal viruses:-

1) Embryonated Chick egg

2)Live animals

3)Tissue culture

The primary purposes of viral cultivation are:

1. to isolate and identify viruses in clinical specimens

2. to prepare viruses for vaccines

3. to do detailed research on viral structure, multiplication cycles, genetics, and effects on host cells.

1.Good Pasteur2.Burnel-Modified3.Animal eggs eg:-CHICKEN,DUCK and TURKEY

4.5to12 days old eggs

Tissues Viruses(cultivated)

Choriallantonicmembrane

Herpes,Small pox virus,Rous Sarcoma virus

Amniotic cavity Mumps virus ,Influenza

Yolk Sac Yellow fever,Rabies

Embryo Influenza ,Mumps

Sites suitable for cultivation of viruses :

Viral culture in eggs: Some viruses, such as influenza viruses, are grown in embryonated chicken eggs