Topic 7: Nucleic Acids Questions

Chapter 7.1: DNA Structure & Replication

11. DBQ: The Hershey-Chase Experiment (pg. 344-345)

a) Supernatant: The soluble liquid fraction of a sample after centrifugation or precipitation of insoluble solids.http://www.biology-online.org/dictionary/Supernatant

b) It should be found in the pellet since it is much heavier in weight than the virus. Thus, the virus will be found in the supernatant since it is much lighter, and is able to float on top of the solution.

c) 80%d) Since most of the genetic material is left in the pellet, it is able to be injected inside the

bacteria cells. Thus, transforming the bacteria into infected cells.

12. Activity: Determining packing ratio (pg. 348)

a) 10:5b) 1:10 000

13. DBQ: Apoptosis and the length of DNA between nucleosomes (pg. 349)

1. i. 250 bp ii. 500 bp iii 750 bp

2. 250bp3. The fragments might become more defined and sharp like the right side, where the

fragments are clearly seen

14. Activity: Analysis of DNA profile (pg. 352-353)

Fragment size (bp)Column 2

Distance moved (mm)Column 2

Fragment size (bp)Column 3

Distance moved (mm)Column 3

60 7070 160130 200

15. DBQ: Analysis of DNA profiles using D1S80 (pg. 353)

a) 123, 246, 369, 492, 615, 738, 861b) G

Distance moved (mm) Fragment size 0.350.801.30

1.802.503.254.20

c)

Individual Distance moved (mm) Estimated fragment size1

2

3

4

5

6

2.42.7

1.73.5

1.42.8

1.11.8

1.23.1

1.61.8

400350

500200

600300

700500

600250

600500

d)