A Nutritional Eye Washing Solution (NEWS) Concentrated that CouldImprove the Sharpness of VisionJohnson K Gao*

Retired from the Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA*Corresponding author: Johnson K Gao, Retired from the Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH, Current address;5609 Harbor Town Drive, Garland, TX 75044, USA, E-mail: jkxgao@gmail.com

Received date: December 02, 2017; Accepted date: Published date:

Copyright: ©use, distribution, and reproduction in any medium.

Abstract

The age-related macular degeneration (AMD) caused vision loss for millions of patient worldly. Lutein andZeaxanthin can reduce the blue light and ultraviolet damage to the retina and decrease rate of AMD. However, luteinis difficult to be dissolved in water, the lutein as a dietary supplement through mouth may be only very small amountthat can reach the eyes. This is a try to use two herbs and four vitamins put in a concentrated nutritional eyewashing solution, and use two drops of that concentrated eye washing solution mixed in every one milliliter of saline,and put in an eye cup to wash the eyes, that may benefit to the easier absorption of those nutritional elements bythe eye. Expectedly, that kind of novel eye washing solution may have some benefit for eye health and a sharpvision.

IntroductionLutein and Zeaxanthin can reduce the blue light and ultraviolet

damage to the retina and decrease the rate of age-related maculardegeneration (AMD) [1]. It is known that AMD had caused vision lossfor millions of people around the world. By feeding the body withantioxidants, such that lutein, it activates the body's self-healingproperty, enable the body to perform detoxin, repair and rejuvenatethe sick cells, thus, it strengthens immune function and even reverseaging. However, lutein is insoluble in water [2]. So, it is difficult todissolve lutein by saline and other aqueous solvent that may greatlydecrease its absorption through digestive system. Free lutein isabsorbed at a higher rate than lutein esters [3].

The eye is about 7~8 g in weight. Two eyes are about 15 g totally. Ifthe average human body is about 50 kg, two eyes are only 15/50,000 ofthe body weight, or, 0.0003 of the body weight. Suppose that one manuptakes a 20 mg lutein pill and if lutein is evenly distributed to thewhole body, two eyes can only get 20 mg × 0.0003=0.006 mg of lutein,or each eye gets 3 µg of lutein. In my preliminary experiment, I learntthat lutein is very difficult to be dissolved in water and alcohol. Theuptaking of lutein by mouth, it might stick to the foods, and only smallamount of lutein can be absorbed by the digestive system. So, it is quitepossible that when a man takes 20 mg lutein pill, one eye can only getabout 1 µg of lutein or even less. And we know that the diameter ofretinal artery, that can transport lutein to the retina, is rather small. So,it is reasonable to consider that eyes are always under the malnutritioncondition of lutein supplying. We may say that aged people, especiallyfor those who have bad circulation or blood vessels cumulated withlipids due to long time of eating bad oily foods are in a state of thirstyof lutein, which will cause blur eye or the ultraviolet light hurt to theeye, or eye pain, or macular degeneration, etc.

Here is a protocol to prepare a nutritional eye washing solution(NEWS). This experiment took six hours to complete (from November10, 2017, 8:00 pm to November 11, 2017, 2:00 am). Previously, I hadtried to prepare eye wash solution containing lutein. However, I feltthat it was very difficult to dissolve lutein in saline or alcohol. With the

successful of this preliminary experiment, I decide to use 1/250 TritonX-100 in 190 proof of Mohawk liquor (grain alcohol) as the organicphase solution to dissolve oily substances.

Preliminary experimentThis pre-experiment is for the selection of the best solvent to

dissolve Lutein:

Figure 1-Explanation of the experiment procedure and result

Figure 1: Solubility of lutein in Mohawk 95% alcohol containingdifferent concentration of Triton X-100.

Add 0.75 ml of Mohawk liquor, 190 proof (95% Alcohol) to eachwell. Add 0.25 ml of Triton X-100 (Sigma Co.) to the well No. 1. Mix itthoroughly. Then, take 0.25 ml liquid out from well No. 1 and transferit to the well No. 2, mix it in the way similarly to well No. 1, andtransfer 0.25 ml of the mixture from well No. 2 to well No. 3, and tomake a diluted series until the well No 5 and discharge the final 0.25 mlof liquid taken from well No. 5. So, the concentration of Triton X-100in 95% alcohol will be that:

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Gao, J Eye Dis Disord 2018, 3:1

Johnson K Gao. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted2018

January 15, 2018; January 21, 2018;

Well No. 1=(1/4) ^ 1, or, 1/4, or, 25% of triton in 95% alcohol.

Well No. 2=(1/4) ^ 2, or, 1/16, or, 6.25% of triton in 95% alcohol.

Well No. 3=(1/4) ^ 3, or, 1/64, or, 1.5625% of triton in 95% alcohol.

Well No. 4=(1/4) ^ 4, or, 1/256, or, 0.3906% of triton in 95% alcohol.

Well No. 5=(1/4) ^ 5, or, 1/1024, or, 0.098% of triton in 95% alcohol.

Use five brush-like tooth pickers and each one was loaded with asmall amount of red-brown color lutein as it was shown on the top ofthe photo, stir each individual lutein-loaded tooth picker in each well.

The best solubility of lutein is in well No. 2 and 3. The well No. 4 isalmost close to totally dissolving with minimum amount of TritonX-100 used. The well No. 5 has a lot of un-dissolved lutein. The wellNo. 1 has small amount of undissolved lutein as well.

Figure 2-The experiment procedure and result

Figure 2: Solubility of lutein in water containing differentconcentration of Triton X-100.

Add 0.75 ml of spring water to each well. Add 0.25 ml of TritonX-100 (Sigma Co.) to the well No. 6. Mix it thoroughly. Take 0.25 mlout and transfer it to the well No. 7, mix it and similarly. Then, transfer0.25 ml out and transfer it to the well No. 8 to make a diluted seriesuntil the well No. 10 and discharge the final 0.25 ml taken from wellNo. 10. So, the concentration of Triton X-100 in water will be that:

Well No. 6=(1/4) ^ 1, or, 1/4, or, 25% of triton in water.

Well No. 7=(1/4) ^ 2, or, 1/16, or, 6.25% of triton in water.

Well No. 8=(1/4) ^ 3, or, 1/64, or, 1.5625% of triton in water.

Well No. 9=(1/4) ^ 4, or, 1/256, or, 0.3906% of triton in water.

Well No. 10=(1/4) ^ 5, or, 1/1024, or, 0.098% of triton in water.

Use five brush-like tooth pickers and loaded with the red-browncolor lutein as it was shown at the top of the photo, stir each individuallutein-loaded tooth picker in each well. Only the well No. 6 the luteinwas partially dissolved. But, lutein in the wells No. 7 to 10 all can't bedissolved.

Figure 3-The experiment procedure and result

Figure 3: Solubility of lutein in Mohawk 95% alcohol containingdifferent concentration of poly-glycerine.

Add 0.75 ml of Mohawk liquor 190 proof (95% Alcohol) to eachwell. Add 0.25 g of poly-glycerine to the well No. 11. It can't bedissolved evenly when it was warned few second in a microwave oven.Take 0.25 ml of warmed and partially dissolved poly-glycerine inalcohol out and transfer it to the well No. 12, mix it well and transfer0.25 ml on the mixture out and transfer it to the well No. 13, and toprepare a diluted series until the well No 15 and discharge the final0.25 ml from well No. 15. So, the concentration of poly-glycerine in95% alcohol will be that:

Well No. 11=(1/4) ^ 1, or, 1/4, or, 25% of poly-glycerine in 95%alcohol.

Well No. 12=(1/4) ^ 2, or, 1/16, or, 6.25% of poly-glycerine in 95%alcohol.

Well No. 13=(1/4) ^ 3, or, 1/64, or, 1.5625% of poly-glycerine in 95%alcohol.

Well No. 14=(1/4) ^ 4, or, 1/256, or, 0.3906% of poly-glycerine in95% alcohol.

Well No. 15=(1/4) ^ 5, or, 1/1024, or, 0.098% of poly-glycerine in95% alcohol.

Use five brush-like tooth pickers and loaded with the red-browncolor lutein as it was shown on the top of the photo, stir eachindividual lutein-carried tooth picker in each well.

None of any wells can dissolve lutein. But, the solution has showncertain yellow color. That means a small amount of lutein is extractedby the poly-glycerine in 95% alcohol. Well 13 and 14 looks less luteinin the well or on the wall of the wells. Actually, the lutein was stickedon the toothpicks and be discarded.

Based on the above three experiments, I decided to use 1/250 TritonX-100 in 95% Mohawk alcohol as the medium to disperse lutein,vitamin A, E, and other organic materials.

The experimental procedure includes the preparation of two phasesolutions. Then, mix the organic phase with the water phase to form aconcentrated "Nutritional Eye Washing Solution". It is a stock solutionand must be stored in a refrigerator. Dilute it ten times with saline toform a working solution.

A. Water phase solution preparation:

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(2018)Gao JK A Nutritional Eye Washing Solution (NEWS) Concentrated that Could Improve the Sharpness of Vision. J Eye Dis Disord: 112. 3

Heat sterilized and grounded to small pieces of stem of Tinosporacordifolia.....4 g

Boric acid................................................0.05 g

Himalayan salt ........................................0.8 g

Aspirin (81 mg/pill) .................................0.081 g

Vitamin C (Pharbest Co. 500 mg/pill) ......0.5 g

Barbering hydrochloride (0.1 g/pill cut half)…......0.05 g

D-sorbitol (Sigma Co.) ..........................................0.01 g

Put the above seven items in a tea bag, cook in 120 ml of boilingspring water for more than10 min. The water will reduce the volume toless than 100 ml passing through a sheet of Whitman No. 1 filter paper.Add more boiled spring water to bring the final volume of 100 ml.

B. Organic phase preparation:

In a small plastic cup, add 10 ml of 1/250 Triton X-100 in MohawkLiquor (190 proof). Then, add with the following items:

Vitamin A (GNC, 10,000 IU/soft gel capsule) ............. 10,000 IU

Vitamin E (Rugby, 400 IU/soft gel capsule)..........................400 IU

Lutein (Spring Valley 20 mg with Zeaxanthin 0.8 mg/gelcapsule).........0.02 g

Borneol................................0.5 g

Musk oil (Planned, but, this time not added, because of supplyunavailable)….5 drops

Phosphatidylserine (GNC, 100 mg/capsule) .............................0.1 g

Warm them in a microwave oven for few seconds to help dissolving.By passing through a filter paper, more than half of the mixture will beadsorbed by the filter paper and can't be recovered. Wash the filterpaper with 2~3 ml of 1/250 Triton X-100 in Mohawk liquor (190proof), bring the final volume to 5 ml.

C. Preparation of the concentrated nutritional eye wash solution:

Add the B (organic phase) solution to A (water phase) solution withvigorous stir. It will form a yellowish turbid solution, which maycontain micelli of lutein, oily vitamins and borneol. Use a sterilizedsyringe to suck 5 ml mixture and let it passing through a 0.2 or 0.45micron film to exclude larger particles and bacteria. Repeat severaltimes of the procedure, until 100 ml of mixture all done. Transfer thesolution into several sterilized small eye-drop plastic bottles. Keepthem in refrigerator.

Add every 2 drops of the "Concentrated Nutritional Eye WashSolution Containing Two Herbal Extracts and Four Vitamins" to 1 mlof saline to form a working solution. You may need to prepare 2~3 mland put it in an eyecup to wash your eyes. Wash the eyes twice or moreper day. The physiological flushing saline, vial of concentrated eyewash solution and eye cup (red scale=2 ml) are shown in Figure 4. Thefinal concentration of lutein is around 20 mg × 1/2 × 1/100 × 1/10, or10 µg/ml, which is several times higher than that the eyes obtaininglutein by oral administration through mouth of lutein 20 mg/tablet,absorbed by the digestive system, and transmitted to the retina.

Figure 4: From left to right: The physiological flushing saline, vial ofconcentrated eye wash solution and eye cup (red scale=2 ml).

Two volunteers, one after glaucoma surgery, Mr. X, age of 84. Hisleft eye is more blur and the image is smaller, only the left eye had usedthe eye wash solution for one week, the right did not do eye-washing asa control. He felt that the image is brighter with the left eye and theimage is larger than before. Another volunteer Mr. Y age of 81, afterright eye retinal pucker removal surgery, he still had blind spot in theleft-down center of the eye for four years, with the original vision only20/200. After he used the eye washing for 7 weeks, his vision improvedto 20/100, and close to 20/75. A self-drawing Amsler chart is shown infigure 5. The side effect had not shown obviously in both twovolunteers. But, it needs to observe continuously.

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J Eye Dis Disord, an open access journal Volume 3 • Issue 1 • 1000112

Gao JK A Nutritional Eye Washing Solution (NEWS) Concentrated that Could Improve the Sharpness of Vision. J Eye Dis Disord(2018): 112. 3

Figure 5: Volunteer Mr. Y self-drawing Amsler Chart. The red circleenclose the area of blind spot before using NEWS, the vision examwas 20/200. The blue circle enclose the area of blind spot after usingNEWS about 7weeks (the last 11 days used 1/250 Triton X-100 in95% Mohawk alcohol to dissolve lutein), the vision exam was20/100. The green circle enclose the area of blind spot after usingNEWS for 8 weeks (the last two weeks used 1/250 Triton X-100 in95% Mohawk alcohol to dissolve lutein), the vision exam was 20/75.

References1. Gary Heiting (2017) Lutein and Zeaxanthin: Eye and Vision Benefits.2. https://pubchem.ncbi.nlm.nih.gov/compound/Lutein#section=Top.3. Paul Krawitz (2017) Free lutein is absorbed at a higher rate than lutein

esters.

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J Eye Dis Disord, an open access journal Volume 3 • Issue 1 • 1000112

Gao JK A Nutritional Eye Washing Solution (NEWS) Concentrated that Could Improve the Sharpness of Vision. J Eye Dis Disord(2018): 112. 3