Hypoxia-inducible regulation of placental BOK expression by Daochun Luo, Isabella Caniggia, and...

Hypoxia-inducible regulation of placental BOK expression

by Daochun Luo, Isabella Caniggia, and Martin Post

Biochem. J.Volume 461(3):391-402

August 1, 2014

©2014 by Portland Press Ltd

Analysis of the human BOK genomic structure (A) Schematic illustration of the human BOK promoter.

Daochun Luo et al. Biochem. J. 2014;461:391-402


EMSA Binding of hypoxia-inducible transcription factors to the BOK-HRE was determined by gel-shift assay.



ChIP assays JEG3 cells were exposed to 21% (lanes 1–5) or 2% (lanes 6–10) O2.



Effect of of HIF proteins on BOK-HRE in the context of a heterologous promoter (A) Schematic illustration of BOK-HRE reporter constructs: control vector SV40-SEAP2, 1.0 kb BOK-SEAP2,

BOK-HRE(+)-SV40-SEAP2, BOK-HRE(−)-SV40-SEAP2, mutated BOK-HRE-SV40-SEAP2 a...



Effect of hypoxia on the activity of the native BOK promoter (A) Schematic illustration of promoterless pSEAP2-basic, 1.0 kb BOK pSEAP2 and its negative control, 0.8 kb BOK-pSEAP2

missing the BOK-HRE.



Effect of hypoxia on the antisense-BOK promoter (A) Schematic illustration of promoterless pSEAP2-basic, 1.7 kb antisense-BOK pSEAP2 and its positive control, 1.0 kb BOK pSEAP2.



Knockdown of the HIF system suppresses the BOK-HRE response to hypoxia HEK-293T (A) or JEG-3 (B) cells transfected with both expression plasmids (pSEAP2-Basic, 0.8 kb-BOK- pSEAP2,

1.0 kb-BOK-pSEAP2 or 1.7 kb-Antisense-BOK-pSEAP2) and siRNAs targeting either...



Expression of antisense-BOK RNA (A) JEG3 cells without treatment of 2% O2 expressed antisense-BOK (400 bp PCR product; lanes 1 and 2).



Hypoxia-inducible regulation of placental BOK expression by Daochun Luo, Isabella Caniggia, and...

Documents

Transcript of Hypoxia-inducible regulation of placental BOK expression by Daochun Luo, Isabella Caniggia, and...