Human cytomegalovirus glycoprotein N polymorphisms among renal transplant recipients in India
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POSTER PRESENTATION Open Access Human cytomegalovirus glycoprotein N polymorphisms among renal transplant recipients in India A Raj Kumar Patro 1,4* , Lalit Dar 1 , Sunil K Pati 1 , Sanjay K Agarwal 2 , Sandeep Guleria 3 , Shobha Broor 1 , Suresh B Boppana 5,6 From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014) Chennai, India. 30 January - 1 February 2014 Background Human Cytomegalovirus (HCMV) is one of the most common infectious complications in renal transplant recipients. Extensive genetic polymorphisms in envelope glycoproteins of HCMV have been demonstrated among clinical HCMV isolates. Some previous studies have pro- posed the potential linkage between HCMV gN geno- type to the frequency of symptomatic infection and clinical outcome in renal transplant recipients. No data available on circulating HCMV strains among renal transplant recipients in India. Objective To determine the cytomegalovirus strain diversity in renal transplant recipients. Methods One hundred thirty two renal transplant recipients were included in this study. DNA extracted from blood sam- ples by Qiagen kit method. Specimen DNA was ampli- fied by PCR using glycoprotein N (gN) gene primers. Genotyping was carried out by RFLP and results were confirmed by direct and/or cloning followed by nucleo- tide sequencing of the plasmid DNA of selected strains. Results HCMV detected by PCR for gN gene in the 83(63%) samples and sixty seven of these samples were typed by RFLP. Among these gN1 was identified in 17(25.3 %) cases. None of the samples contained gN2 genotype, gN3 genotype detected in 9(13.4%) cases, while gN4 strain was identified in 23 cases (34.3%), respectively. Infection with multiple gN type was seen in 18(27 %) of the samples. Conclusion The results of our study showed that gN4 was the com- monest gN genotype found followed by gN1. This study was supported by the Indian Council of Medical Research, Government of India. Authors’ details 1 Departments of Microbiology, All India Institute of Medical Sciences, New Delhi, India. 2 Departments of Nephrology, All India Institute of Medical Sciences, New Delhi, India. 3 Departments of Surgery, All India Institute of Medical Sciences, New Delhi, India. 4 Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, India. 5 Departments of Pediatrics, University of Alabama at Birmingham, Birmingham, USA. 6 Departments of Microbiology, University of Alabama at Birmingham, Birmingham, USA. Published: 27 May 2014 doi:10.1186/1471-2334-14-S3-P66 Cite this article as: Patro et al.: Human cytomegalovirus glycoprotein N polymorphisms among renal transplant recipients in India. BMC Infectious Diseases 2014 14(Suppl 3):P66. * Correspondence: [email protected] 1 Departments of Microbiology, All India Institute of Medical Sciences, New Delhi, India Full list of author information is available at the end of the article Patro et al. BMC Infectious Diseases 2014, 14(Suppl 3):P66 http://www.biomedcentral.com/1471-2334/14/S3/P66 © 2014 Patro et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Transcript of Human cytomegalovirus glycoprotein N polymorphisms among renal transplant recipients in India
POSTER PRESENTATION Open Access
Human cytomegalovirus glycoprotein Npolymorphisms among renal transplant recipientsin IndiaA Raj Kumar Patro1,4*, Lalit Dar1, Sunil K Pati1, Sanjay K Agarwal2, Sandeep Guleria3, Shobha Broor1,Suresh B Boppana5,6
From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014)Chennai, India. 30 January - 1 February 2014
BackgroundHuman Cytomegalovirus (HCMV) is one of the mostcommon infectious complications in renal transplantrecipients. Extensive genetic polymorphisms in envelopeglycoproteins of HCMV have been demonstrated amongclinical HCMV isolates. Some previous studies have pro-posed the potential linkage between HCMV gN geno-type to the frequency of symptomatic infection andclinical outcome in renal transplant recipients. No dataavailable on circulating HCMV strains among renaltransplant recipients in India.
ObjectiveTo determine the cytomegalovirus strain diversity inrenal transplant recipients.
MethodsOne hundred thirty two renal transplant recipients wereincluded in this study. DNA extracted from blood sam-ples by Qiagen kit method. Specimen DNA was ampli-fied by PCR using glycoprotein N (gN) gene primers.Genotyping was carried out by RFLP and results wereconfirmed by direct and/or cloning followed by nucleo-tide sequencing of the plasmid DNA of selected strains.
ResultsHCMV detected by PCR for gN gene in the 83(63%)samples and sixty seven of these samples were typed byRFLP. Among these gN1 was identified in 17(25.3 %)cases. None of the samples contained gN2 genotype,
gN3 genotype detected in 9(13.4%) cases, while gN4 strainwas identified in 23 cases (34.3%), respectively. Infectionwith multiple gN type was seen in 18(27 %) of the samples.
ConclusionThe results of our study showed that gN4 was the com-monest gN genotype found followed by gN1. This studywas supported by the Indian Council of Medical Research,Government of India.
Authors’ details1Departments of Microbiology, All India Institute of Medical Sciences, NewDelhi, India. 2Departments of Nephrology, All India Institute of MedicalSciences, New Delhi, India. 3Departments of Surgery, All India Institute ofMedical Sciences, New Delhi, India. 4Department of Microbiology, All IndiaInstitute of Medical Sciences, Bhubaneswar, India. 5Departments of Pediatrics,University of Alabama at Birmingham, Birmingham, USA. 6Departments ofMicrobiology, University of Alabama at Birmingham, Birmingham, USA.
Published: 27 May 2014
doi:10.1186/1471-2334-14-S3-P66Cite this article as: Patro et al.: Human cytomegalovirus glycoprotein Npolymorphisms among renal transplant recipients in India. BMCInfectious Diseases 2014 14(Suppl 3):P66.
* Correspondence: [email protected] of Microbiology, All India Institute of Medical Sciences, NewDelhi, IndiaFull list of author information is available at the end of the article
Patro et al. BMC Infectious Diseases 2014, 14(Suppl 3):P66http://www.biomedcentral.com/1471-2334/14/S3/P66
© 2014 Patro et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
