Huacaya BVDV in Alpaca: Abortion and Canada 17,000 ... · PDF fileBVDV in Alpaca: Abortion and...
Transcript of Huacaya BVDV in Alpaca: Abortion and Canada 17,000 ... · PDF fileBVDV in Alpaca: Abortion and...
BVDV in Alpaca: Abortion and
Persistent Infection
Susy Carman 1Murray Hazlett 1
Josepha Delay 1Nancy Carr 2Mohit Baxi 3
Dirk Deregt 3
Huacaya
Canada 17,000Ontario 1,300
Tested 101 alpaca28 herds
5 BVDV infected3 BVDV PI2 PI studied
Suri
University of Guelph 1Elginburg,ON 2
Canadian Food Inspection Agency 3
Denver, 2006
Alberta 1 Alberta 2 Ontario 4
Ontario 552 alpaca
9 sick, 1 diedstress breaks
fleece
Aborted Fetus5.5 mon
IHC lu,ki,ht + BVDVVI + NCP-BVDV skin only
17/20 -Type 1 NADL - 1:3072Type 2 NVSL 125c - 1:394
2 early pregnancy
losses 75-80d gest
13 cria born1 Low birth weight
BVDV PIDiarrhea
2.5 mon
9 m
on3 mon
Alberta 3
Gabriel
Sickly criaLeBron born3,500 km
LeBron died 8 monPneumonia, Diarrhea
5 mon
abort/still/neo/BVDVAb
Ontario 6Aborted Fetus
7 mon
6 mon
PregnantFemale
Herd History PI Cria1
3 mon
East
Gabriel’shuacaya farm
52 alpaca
Dam & cria barn facing south
Fleece storage
Courtesy Nancy Carr
Herd History PI Cria 21,500 km
Ontario 764 alpaca
New Brunswick 997 alpaca
2 abortions
9/10 - Type 1 NADL - 1:384Type 2 NVSL 125c - 1:96
21 tested1 Low birth weight cria
PI BVDV
PregnantFemale
4 mons ges
Lilahuacaya
4monthsPneumonia, Diarrhea
Ontario 833 alpaca
Lila - PIGabriel - PI● Exposed before120d gestation● Exposed about 65d gestation
(Dam Ab Type 1, 1:768)
● NCP-BVDV iso 75d buffy coat; 75d,104d,120d serum; 120d tissues
● NCP-BVDV iso from placenta;3d, 26d, 46d buffy coat; 46d tissues
● RT-PCR - BVDV Type 1, - whole blood-/+plasma, -/+serum, + buffy coat
● RT-PCR - BVDV Type 1,-/+/- serum 120d
● Ag ELISA 46d + serum, + ear notch ● Ag ELISA 120d +serum
● Serum virus titer 105.0 CCID50 /mL● Serum virus titer 104.0 CCID50 /mL
● Serum Ab titer 120d - neg Type 1, 2● Serum Ab titer 46d - neg Type 1,2
ItalyPader
V 60
Gifhorn
T1802
V2536BDV
SCPGi6
CD87
125c
BVDV24812
SD-1
C86
NADLSinger
OslossCP7
Hastings
BVDV1b
Alpaca-24913
BVDV1a
H138
Giraffe
Eystrup
CSFV
100
100
100
97100
100
9991
57
100
8988
7590
6262
100
Gabriel Type1b - 4 NA / 2AA diff
Courtesy of Dr. Mohit Baxi
PI Cria 1 BVDV E2 gene
Lila AxillaGabriel Ear
Hair follicle
Skin
Gabriel Lila
Brain
Retina
Gabriel
Thymus
Lila
BoneMarrow
Gabriel Lila
Compartment 3Stomach
Colon
Gabriel Lila
Kidney
Chorioallantoic membraneplacenta
Need to update recommendations for testing before next alpaca birthing season
(1 vs 16 or 39 BVDV PI)
• Test/specimen to identify BVDV PI cria?
• VI +/+ buffy coat • RT-PCR + buffy coat• IHC + placenta• IHC +/+ haired skin• Ag ELISA +/+ serum, + haired skin• Multiple tests on low birth weight cria?
• Vaccination? – assessment of herd exposure
• Cerebellar Hypoplasia? • Mucosal Disease?
Thyroidgland
LilaGabriel
Alberta 1 Alberta 2 Ontario 4
Ontario 552 alpaca
9 sick, 1 deadstress breaks
fleece
Aborted Fetus5.5 mon
IHC lu,ki,ht + BVDVVI + NCP-BVDV skin only
14/17 -Type 1 NADL - 1:3072Type 2 NVSL 125c - 1:394
2 early pregnancy
losses 75-80d gest
13 cria born1 Low birth weight
PI BVDVDiarrhea
2.5 mon
9 m
on3 mon
Alberta 3
Gabriel
Sickly criaLeBron born3,500 km
LeBron died 8 monPneumonia, Diarrhea
5 mon
abort/still/neo/BVDVAb
Ontario 6Aborted Fetus
7 mon
6 mon
Female
Herd History PI Cria1
3 mon