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![Page 1: Http://oilab.seas.wustl.edu -- 1 In Vivo Photoacoustic Imaging: Brain Research Application Vassiliy Tsytsarev, E-mail: tsytsarev@umaryland.edutsytsarev@umaryland.edu.](https://reader035.fdocuments.us/reader035/viewer/2022062515/56649d095503460f949db673/html5/thumbnails/1.jpg)
http://oilab.seas.wustl.edu -- 1
In Vivo Photoacoustic Imaging:Brain Research Application
In Vivo Photoacoustic Imaging:Brain Research Application
Vassiliy Tsytsarev,
E-mail: [email protected]
University of Maryland School of Medicine
Most of the presented data have been obtained in:
Optical Imaging Laboratory
Department of Biomedical Engineering
Washington University, St. Louis
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http://oilab.seas.wustl.edu -- 2
What is Photoacoustic?What is Photoacoustic?
Photoacoustic imaging - a hybrid biomedical imaging modality, is developed based on the photoacoustic effect
Photoacoustic effect – discovered by Alexander Bell in 1880; he showed that thin discs emitted sound when exposed to a beam of sunlight that was rapidly interrupted with a rotating slotted disk.
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http://oilab.seas.wustl.edu -- 3
How it works?How it works?
Laser
Ultrasound
Ultrasound trasducer
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http://oilab.seas.wustl.edu -- 4
PA Imaging: three types of scanningPA Imaging: three types of scanning
PACT image of the cortical vasculature in a living adult intact rat
Dark-field AR-PAM OR-PAM (Open Brain)
(Wang et al., 2003; Stein et al., 2008)
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http://oilab.seas.wustl.edu -- 5
Wavelength-tunable laser system
Condenser lens
Pinhole
Microscope objectiveCorrection lens
Ultrasonic transducer
Right-angle prismSilicone oil layer
Water tank
ScannerPolyethylene membrane
Acoustic lens
Scheme of optical-resolution photoacoustic microscopy (OR-PAM)
Scheme of optical-resolution photoacoustic microscopy (OR-PAM)
Hu et al, 2007; (Wang et al., 2003; Stein et al., 2008)
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http://oilab.seas.wustl.edu -- 6
Intact Rat PA ImagingIntact Rat PA Imaging
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http://oilab.seas.wustl.edu -- 7
Open Brain PA ImagingOpen Brain PA Imaging
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http://oilab.seas.wustl.edu -- 8
Functional Brain Imaging: Transcranial ApplicationFunctional Brain Imaging: Transcranial Application
(Hu and Wang, 2009)
In vivo PACT image of the cerebral vascular response to right-side whisker stimulation (intact rat)
The hemodynamic response due to whisker stimulation is shown in blue and red and is superimposed on the cortical vascular image shown in gray.
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http://oilab.seas.wustl.edu -- 9
PA Microscopy: 2-Wavelengths Functional ImagingPA Microscopy: 2-Wavelengths Functional Imaging
Hu, Maslov, Tsytsarev and Wang, 2009
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http://oilab.seas.wustl.edu -- 10
Photoacoustic imaging of the vascular responsePhotoacoustic imaging of the vascular response
Real-time monitoring of photoacoustic signals at specific excitation wavelengths reveals vascular dynamics, such as changes in blood volume and oxygen saturation, in response to electrical or physiological stimulation or epileptic seizures
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http://oilab.seas.wustl.edu -- 11
Neurovascular coupling: response to electrical stimulation
Neurovascular coupling: response to electrical stimulation
AstrocyteNeuron
Electrode
Electrical stimulation may cause neurons to release various neurotransmitters (Glutamate [Glu], GABA, ATP and NO). These reactions drive the vessel to either vasoconstriction or vasodilatation
GABA
Glu
Glu
K+, NO
ATP
Blood vessel
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http://oilab.seas.wustl.edu -- 12
Photograph of exposed mouse brain surface with introduced microelectrode
Photograph of exposed mouse brain surface with introduced microelectrode
Microelectrode
Cranial opening
5 mm
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http://oilab.seas.wustl.edu -- 13
Photoacoustic imaging of the brain microvasculature
Photoacoustic imaging of the brain microvasculature
Photograph of the microelectrodeMin Max
1.0
SO
2
1mm
The oxygen saturation (SO2) mapping is shown as a superposition in color scale. A line-scan monitoring of the vascular response was performed along the dashed yellow line.
400 μmOptical absorption
0.6
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Vascular response to electrical stimulationVascular response to electrical stimulation
Transverse
Axi
al
10 μm
Transverse
Axial
Stimulation
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Vascular response to electrical stimulationVascular response to electrical stimulation
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Vasoconstriction and vasodilatationVasoconstriction and vasodilatation
50 s
130 s
230 s
150 s
250 s
10 s
50 s
126 s
217 s
150 s
280 s
25 µm50 µm Optical absorptionMin Max
Electrical stimulations
150 µA100 µA
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Time courses of the vessel cross-sectional area under various stimulation intensities
Time courses of the vessel cross-sectional area under various stimulation intensities
Vasoconstriction and vasodilatation are observed, and the response duration is positively correlated with stimulation intensity
Stimulation 1 Stimulation 2Each stimulation consisted of a train of four 0.3 ms pulses at 300 Hz
150 μA300 μA
350 μA
110 μA
100 μA
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http://oilab.seas.wustl.edu -- 18
Transcranial imaging of the single blood vesselTranscranial imaging of the single blood vessel
400 μm
Studiedvessel
Stimulation
0 10 20 time (s)
1.6
1.4
1.2
1.0
medial
rostral
Transcranial images of stimulation-induced vasodilatation
a) Transcranial brain imageb) Crossectional monitoringc) Time courses of the electrical-
stimulation-induced vessel size
(a) (b)
(c)
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http://oilab.seas.wustl.edu -- 19
Epileptic Seizures: Single Vessel MonitoringEpileptic Seizures: Single Vessel Monitoring
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SummarySummary
Optical-resolution photoacoustic microscopy (OR-PAM) imaged two types of vascular response to electrical stimulation:
Vasoconstriction Vasodilatation
OR-PAM clearly and reliably imaged the vascular response to electrical stimulation at the capillary level with a temporal resolution of one second.
OR-PAM is a promising tool for in vivo studies of neurovascular coupling under a variety of experimental conditions invasively as well as transcranially
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http://oilab.seas.wustl.edu -- 21
Epileptic seizures accompanied by two vessels vasodilatation (PA Imaging)
Epileptic seizures accompanied by two vessels vasodilatation (PA Imaging)
Vessel 1
Vessel 2
EEG
10 s / 2 mV
25 μm
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Conlusions and Perspecives:Conlusions and Perspecives:
- Current application: animal experiment- Clinical application: questionable
- Perspective: biomarcer hybridozation