Homogenizers introduction
13
Click here to load reader
Transcript of Homogenizers introduction
Lab equipment and reagents
www.wisbiomed.com 1-866-692-1249
WISBIOMED
-bead based tissue homogenizers
Bead Based Tissue Homogenizers
www.wisbiomed.com 1-866-692-1249
High density beads in a closed sample chamber with tissue
samples
Homogenizer agitate sample vessels
Beads in the sample chamber move fast to shred tissue
samples, or grind up sample pieces
Types of Grinding Beads
www.wisbiomed.com 1-866-692-1249
Stainless steel: high density
Zirconium Oxide: high density, inert surface
Zirconium Silicate:
Glass:
Density: stainless steel>Zirconium Oxide>Zirconium
Silicate>Glass
Types of sample chamber movement
www.wisbiomed.com 1-866-692-1249
Linear motion: entire sample chamber moves front left to
right or up down. HT-01 Homogenizer
S shape motion: TissueLyzer, GenoGrinder
3D motion: Precellys, Minilys, Powerlyzer, TissueRaptor
Sample size and through-put
www.wisbiomed.com 1-866-692-1249
Low through-put: one to three samples per run: BeadBug,
Minilys, CryoGrinder
High through-put: Precellys, BulletBlender
Sample tube size:
Single tube design: for optimal performance
Flexible tube size: defined sample chamber size, fit different
size tubes, plates
Combination design: for different size tubes
Motion Speed
www.wisbiomed.com 1-866-692-1249
Low speed: less than 2000 RPM: BeadBug, BulletBlender,
HT-01
High speed:Minilys, Precellys,
Variable speed: user set speed: Precellys
Cooling Design
www.wisbiomed.com 1-866-692-1249
No cooling and require softened tissue samples to start with:
BeadBug, Minilys, BulletBlender
No cooling, but work with frozen solid tissue samples:
Minilys, Precellys
Built-in dry ice cooling: BulletBlender Gold
Attached dry ice or liquid nitrogen cooling: Precellys with
Cryolys
Low through put homogenizers
www.wisbiomed.com 1-866-692-1249
BeadBug: for one to three 2ml tube, room temperature
operation
Minilys: for upto three 2ml tubes, or one 7ml tube
CryoGrinder: manually process one sample at a time, work
with CryoCooler with liquid nitrogen
www.wisbiomed.com
Bullet Blender Storm
www.wisbiomed.com 1-866-692-1249
Models for 2ml tubes, 5ml tubes and 50ml tubes
Each works only one type of tube size
Up to 24 samples per run
Built-in cooling fan to facilitate air circulation
www.wisbiomed.com
Bullet Blender Gold
www.wisbiomed.com 1-866-692-1249
Models for 2ml, 5ml or 50ml tubes
With built in dry ice bucket for cooling
Fixed tube size design
Extra sound insulation resulting a quiet operation
www.wisbiomed.com
High Through Put Homogenizer
www.wisbiomed.com 1-866-692-1249
Fixed sample chamber for 2ml, 5ml, 15ml or 30ml or 96
well plates
Linear motion
Room temperature operation
website
Precellys Homogenizer
www.wisbiomed.com 1-866-692-1249
Models for 2ml tubes, 7ml tubes and 0.5ml tubes
High through put
Speed from 5000 to 6500 RPM
Optional cooling unit with dry ice or liquid nitrogen
www.precellys.com
Pre-filled Tubes for homogenization
www.wisbiomed.com 1-866-692-1249
Pre-filled tubes with beads
Sterilized, RNase free, DNase free
Bulk packaged beads and tubes
www.wisbiomed.com
