HIV GP120 Envelope Protein
description
Transcript of HIV GP120 Envelope Protein
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HIV GP120 Envelope Protein
Objectives :
• Observing consensus sequences of different strains
• Structure analysis
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GP120 FunctionGP120 involved in infection of human T-cells
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HIV TYPES
HIV Type IHIV Type II
HIV Type III
SIV
Human viruses Simian virus
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GP120 Sequence analysisSequence analysis shows a high mutation rate with preserved large consensus regions not only between strains, but also between different types
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GP120 Sequence analysis
HIV Type I shows additional sequence of 7 aa in it’s consensus sequence in a consensus region in acids 27-33
consensus LWVTVFYGVPVWKEATTTLFCATDNK-------NTWATHACVPTDPDYQEVELNNVTENF 53
HIV1 LWVTVYYGVPVWHDADPVLFCASDAKahsteahNIWATQACVPTDPSPQEVFLPNVIESF 92
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Two structures of GP120-CD4 from HIV 1 were analyzed
Hiv-1 Yu2 isolate Hiv-1 Hxbc2 isolate
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Difference between the proteins
Score = 566 bits (1460), Expect = e-161Identities = 276/321 (85%), Positives = 289/321 (89%), Gaps = 8/321 (2%)
The similarity is shown, using protein blast
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Structure of conserved region
GP120 Hxbc2 isolatePreserved region marked in yellow
CD4
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Docking of GP120
Clustered solution ofDimers
With current approach GP120 exists as atrimers or tetramers on the membrane surface.According to J. Biol. Chem., Vol. 275, Issue 45, 35137-35145, November 10, 2000
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Conclusions1. Preserved sequences found
2. GP120-CD4 theoretical docking performed, clustered solutions found
3. The results provide a basis for further research