High Through Put Tissue Homogenizer

Process 96 wellplate in minutes.Get bone, hair, plants, corn pulverized easily.

The HT Homogenizer is the smallest, most powerful linear motion homogenizer available. It is also the least expensive costing $3500 less than comparable models. This unit was designed specifically for laboratories that require high throughput homogenization of samples in 96 well, microplate format while limiting well-to-well variation. This homogenizer is proven for disrupting samples for DNA, RNA, and protein isolation procedures.

The HT Homogenizer was born from the request of a pathologist that needed an effective high throughput homogenization tool that could

The HT Homogenizer is the smallest, most powerful linear motion homogenizer available. It is also the least expensive costing $3500 less than comparable models. This unit was designed specifically for laboratories that require high throughput homogenization of samples in 96 well, microplate format while limiting well-to-well variation. This homogenizer is proven for disrupting samples for DNA, RNA, and protein isolation procedures. The HT Homogenizer was born from the request of a pathologist that needed an effective high throughput homogenization tool that could be easily transported to the field for surveying zoonotic viruses. Powered with a strong 1/4 horsepower motor, the HT Homogenizer has been successfully used to homogenize tough samples such as corn, rice, soybean, and Arabidopsis callus. It is also effective at homogenizing softer tissues from animals and leaf punches. When used with silica and zirconium grinding media, the HT Homogenizer is also effective in disrupting microorganisms.

be easily transported to the field for surveying zoonotic viruses. Powered with a strong 1/4 horsepower motor, the HT Homogenizer has been successfully used to homogenize tough samples such as corn, rice, soybean, and Arabidopsis callus. It is also effective at homogenizing softer tissues from animals and leaf punches. When used with silica and zirconium grinding media, the HT Homogenizer is also effective in disrupting microorganisms. High throughput homogenization is valuable for any labs processing multiple samples for RNA extraction, DNA isolation, or protein analysis. Samples homogenized can be handled with workstations for subsequent processing and purfication. High throughput homogenization is valuable for any labs processing multiple samples for RNA extraction, DNA isolation, or protein analysis. Samples homogenized can be handled with workstations for subsequent processing and purfication. High throughput homogenization is valuable for any labs processing multiple samples for RNA extraction, DNA isolation, or protein analysis. Samples homogenized can be handled with workstations for subsequent processing and purfication.

Standard, half, and deep well plates, as well as vial sets, are locked into the homogenizers carriage assembly with a lid with adjustable latches. This allows for easy placement and removal of plates. Most samples can be homogenized in 2 minutes or less, making preparation and loading of the plate the bottleneck in sample preparation and not the homogenizer. The HT Homogenizer is CE and CSA certified. This product comes with a 2 year limited warranty.

Speed Range: 50-1600 rpm

Controls: Analog with a 10 min. timer

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Standard, half, and deep well plates, as well as vial sets, are locked into the homogenizers carriage assembly with a lid with adjustable latches. This allows for easy placement and removal of plates. Most samples can be homogenized in 2 minutes or less, making preparation and loading of the plate the bottleneck in sample preparation and not the homogenizer. The HT Homogenizer is CE and CSA certified. This product comes with a 2 year limited warranty.

Speed Range: 50-1600 rpm

Controls: Analog with a 10 min. timer

Find Out more

Standard, half, and deep well plates, as well as vial sets, are locked into the homogenizers carriage assembly with a lid with adjustable latches. This allows for easy placement and removal of plates. Most samples can be homogenized in 2 minutes or less, making preparation and loading of the plate the bottleneck in sample preparation and not the homogenizer. The HT Homogenizer is CE and CSA certified. This product comes with a 2 year limited warranty.

Speed Range: 50-1600 rpm

Controls: Analog with a 10 min. timer

Find Out more

These two grinder homogenizers are very effective at homogenizing a variety of samples in high throughput formats. Unlike other ball mills that move in a “figure 8” pattern, these units shake samples in a linear motion. This creates processing uniformity between samples. Both units are designed to hold standard plates, deep well plates, vial sets, and

small racks with microfuge or other small tubes. The Geno/Grinder can further be adapted to hold 15 or 50 ml conical tubes (adaptor cost is approximately $1000). The following are images of each unit.

Troemner HT Homogenizer: The unit is smaller and lighter than the Geno/Grinder 2000. Capacity is one plate or vial set.

SPEX CertiPrep Geno/Grinder 2000: Plates are clamped two deep in the raised platform.

Both units function well and homogenize tissue with equal results. In functional tests, both units can effectively grind dry corn and soy to meal. Using small beads, microorganisms can be effectively cracked with both units. Both units also effectively homogenize animal tissue. With both units, sample size determines homogenization format. Leaf punches and tissue samples less than 50 mg can be processed in deep well plates while seeds and larger plant and animal samples require vial sets. Batch samples can be processed in 15 ml vial sets.

The essential differences between the units are with plate capacity, tube capabilities, latching method, timer and speed. The two plate capacity of the Geno/Grinder may be a plus for some laboratories, but most researchers will find that homogenization normally requires no more than 2 minutes while loading the plate may take an hour or longer. Consequently, the bottle neck for sample throughput is not the grinding, but rather loading the plate. The Geno/Grinder also has the option of fitting the unit with 15 or 50 ml tube adaptors. The value of this feature is questionable as the stroke of both units is optimized for the deep well plate and vial sets. The relatively long length of conical tubes (4.75 in.) means that the grinding ball is usually in limbo while with the shorter plates and vials the ball is going up just when the sample is coming down. This provides added force during the homogenization.

The method of securing plates and tubes in the units differs as summarized below.

The mechanism for securing plates into the HT Homogenizer employs an adjustable latch. This latch can be expanded as needed. Maximum height of samples is 2.75 inches.

The Geno/Grinder has a fixed latching system. For short plates and tubes, spacers must be fitted under the plates in order to securely fix the samples in place. Maximum height of samples is 2 inches.

The timer mechanism for the two grinders is also different. The Geno/Grinder has a digital timer that can run up to 99:59 minutes. Alternatively, the HT Homogenizer has digital circuitry controlled by a dial timer that runs up to 10 minutes. The timer difference is a matter of practicality and safety. Nearly all samples will be homogenized in less than 2 minutes. Batch samples, such as corn kernels in 15 ml vial sets, may require 5 minutes of homogenization. The Geno/Grinder uses a timer that is a component piece, i.e., off the shelf, thus it can be set for

prolonged times. However, using it in this capacity is not necessarily a good idea. The HT Homogenizer has a timer which is designed to limit grinding runs to 10 minutes. The rationale for limiting the run length is based on the concept that plates and vials may not hold up for prolonged periods of bead beating.

The HT Homogenizer has a greater speed range that the Geno/Grinder 2000. With a lower setting of 50 rpm, the HT Homogenizer can be used for mixing and extraction as well as grinding at higher speeds. The upper range of the Geno/Grinder at 1500 rpm is sufficient for grinding the hardest of samples, such as dried seeds, however the added speed of the HT Homogenizer at 1600 rpm accomplishes this task in less time. For practical purposes, the HT Homogenizer can be run at 1250 rpm, a speed that will prolong the life of the unit.

TABLE: Features Comparison of the Geno/Grinder 2000 and HT Homogenizer

Feature HT Homogenizer Geno/Grinder 2000 List Price $6460 $10450 Controls Dials with digital circuitry Digital timer and dial speed

control Speed 50-1600 rpm 600-1600 rpm Timer 0-10 min. 0-99:59 min. Capacity One deep well plate

Five stacked standard plates

One rack with tubes (5 ml or microfuge tubes).

Two deep well plates

Eight stacked standard plates

Two racks with small tubes

Adaptors are available for 15 ml and 50 ml tubes.

Weight 70 lbs. 105 lbs. Dimensions 17 L x 15 W x 15 H 16 L x 20 W x 16 H Motion Linear/Horizontal Linear/Vertical Power Requirements 115 V (also available in 230 V) 115V (also available in 230 V) Demonstration Unit Free Shipping Shipping Charge ($300-600)

**Comparison was made from David Burden