Hematoxylin and Eosin...
Transcript of Hematoxylin and Eosin...
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Hematoxylin and Eosin (H&E)
� The Hematoxylin is a basic dye that stains acidic
components of cells a blue color.
� This characteristic is known as basophilia.
� Hematoxylin stains the nuclei of cells, and the RER of
the cytoplasm.
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� Eosin is an acidic dye that stains the basic
components of the cells a reddish-pink color.
� This characteristic is known as acidophilia.
� Most of the cytoplasm of cells is stained by eosin.
� Bone matrix is also stained by eosin.
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Periodic acid-Schiff (PAS) staining
� PAS is a widely used staining technique that stains the
neutral sugars of glycosaminoglycans a pink color.
� Common components stained positively with PAS
include mucus, glycogen, muco-protein, glycoprotein,
the basal lamina, organ capsules, blood vessels, etc.
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Orcein
� Orcein staining is used to stain elastic fibers a dark
brown-purple color.
� This is used, for example, to show the elastic
components in the walls of arteries, or in the matrix
of elastic cartilage.
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Osmium tetroxide
� Osmium is used to stain lipids a dark black color.
� It is very useful for demonstrating the myelin of
myelinated nerves, or lipid droplets in the liver or
steroid-secreting cells.
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Toluidine blue
� Toluidine blue is a so-called metachromatic stain.
� It is a blue stain that stains specific components of tissues
a purple color.
� This change in staining color known as metachromasia.
� Metachromasia is seen in the matrix of hyaline cartilage,
or in the granules of mast cells.
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Impregnation
� Impregnation is a staining technique in which
blocks of tissue are processed in solutions
containing metals such as silver or gold, which
attach to specific components in tissues.
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� The silver or gold are then further processed
(reduced) and develop into dark metallic deposits.
� The stained blocks are then sectioned.
� Silver impregnation is widely used to stain
neurons and to demonstrate reticular fibers.
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Vital staining
� Vital staining refers to the uptake of dyes by cells.
� If we inject Trypan blue into experimental animals,
the dye is rapidly engulfed by specific macrophages.
� We can use such vital staining to demonstrate the
Kupffer cells of the liver.
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Giemsa stain
� There are a variety of "Romanowsky-type" stains with
mixtures of methylene blue, azure, and eosin
compounds.
� Among these are the Giemsa stain and the Wright's stain
(or Wright-Giemsa stain).
� The latter is utilized to stain peripheral blood smears.
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Connective tissue stains
� The trichrome stain helps to identify the supporting
collagenous stroma in sections from a variety of organs.
� Trichrome helps in identifying normal structures, such
as connective tissue capsules of organs, the lamina
propria of gastrointestinal tract, and the broncho-
vascular structures in lung.
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Malory stain
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Masson`s trichrome
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� Examples of trichrome stain:
�Malory stain: stains collagen blue
�Masson`s trichrome: stains collagen
green