Heat tolerance QTL in wheat - IWGS 2017€¦ · Heat tolerance QTL in wheat Nick Collins,...
Transcript of Heat tolerance QTL in wheat - IWGS 2017€¦ · Heat tolerance QTL in wheat Nick Collins,...
![Page 1: Heat tolerance QTL in wheat - IWGS 2017€¦ · Heat tolerance QTL in wheat Nick Collins, University of Adelaide International Wheat Genetics Symposium, Tulln, April 2017. Impact](https://reader036.fdocuments.us/reader036/viewer/2022063016/5fd57982ac93c44b8363f0eb/html5/thumbnails/1.jpg)
Heat tolerance QTL in wheat
Nick Collins, University of AdelaideInternational Wheat Genetics Symposium, Tulln, April 2017
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Impact of heat waves on wheat yield in southern Australia
Data used from 600 southern-Australian NVTs, 2005-2010
Paul Telfer et al. 2015; AGT
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Greenhouse/chamber assays
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Our two greenhouse/chamber assays
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Waagan x Drysdale heat tolerance QTL
Grain filling tolerance and stay green
Floret fertility tolerance
2B 3B 6B
Ppd-B1
1B 7A
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Floret sterility is associated with an absence of starch in mature pollen grains
Drysdale(intolerant)
Waagan(tolerant)
Control Heat
Iodine staining
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Dominance of the 2B-QTL (grain set)
a a a a a a
b b
c
b b
c
Family 1 Family 2
2B-QTL genotype:
DW DDWW
Score grain set
DW (heterozygous RIL plant)
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Dominance of the 2B-QTL (pollen)
a a a aa
b
Score % starchy pollen
Intolerance may result from a loss of gene function
DW DDWW
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Does the 2B-QTL tolerance affect female reproduction?
n=41
n=51
n=64n=61
n=61n=50 n=63
n=24
n=40
Family 1 Family 2 Family 3
2B-QTL genotype:
Emasculate
Apply pollen fromnon-heated plant
Score grain set
DW DDWW
No indication that it does (or at least to the same extent as it does the pollen)
![Page 10: Heat tolerance QTL in wheat - IWGS 2017€¦ · Heat tolerance QTL in wheat Nick Collins, University of Adelaide International Wheat Genetics Symposium, Tulln, April 2017. Impact](https://reader036.fdocuments.us/reader036/viewer/2022063016/5fd57982ac93c44b8363f0eb/html5/thumbnails/10.jpg)
Is the 2B-QTL tolerance the result of gene expression in haploid cells?
Family 1 Family 2
Heterozygote:
Progeny:
heat
DW
DW DD DW DD WW
Determine overall marker allele frequencies
No.Heat-stressing F1 hybrids will not work as a
selection tool for heat tolerance breeding
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Mitotic divisions in (haploid) microspores
Image: Borg and Twell, 2011
Meiosis
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Mitotic divisions were not affected...
‘Normal’ tri-nucleate pollen (DAPI staining):
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Susceptible stage in cv. Drysdale
Early meiosis
Pre meiosis Meiosis Tetrad Early uninucleate
Late uninucleate
Binucleate Trinucleate
(80-90% of final spike length)
Starch accumulation normally begins
Tapetum degradation normally begins
Tolerance is suspected as being the result of gene action in the tapetal cells
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Linkage to rust resistance genes (Drysdale x Waagan population)
D W
Yr27Lr23? Heat tolerance
2BSD W
• Rust resistance • Pseudo black chaff• Heat induced seedling chlorosis
Heat tolerance, staygreen
3BS
Sr2
D W
Heat tolerance, staygreen
6BS
No rust resistance?
Fertility toleranceGrain filling tolerance
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ARC Industrial Transformation Research Hub forWheat in a Hot and Dry Climate
2016-2020• Univ. Adelaide (ACPFG), Univ. SA, Univ. Sydney• GRDC• AGT, Longreach, InterGrain NILs for heat tolerance QTLs will be field-
trialled under regular rain fed conditions.
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Response to heat shock – senescence in the grain
heat
Dry weight per grain (mg)
Senescence gene A
Senescence gene A
Shirdelmoghanloo et al. 2016. Functional Plant Biology 43:919-930Shirdelmoghanloo et al. 2016. Acta Physiol Plant 38:208
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Acknowledgements
Nick CollinsHamid ShirdelmoghanlooMillion ErenaMizanur RahmanIman LohrasebSigrid HeuerDiane MatherJulian TaylorSabela Munoz-Santa
Livinus EmebiriPeter Martin