BioNordika Finland Oy • tel 0207 410 270 • info@bionordika.fi • www.bionordika.fi

November - December 2019

Issue 3

Happy birthday NEBNext!

NEB’s NGS-dedicated product line, NEBNext, is turning 10 years this year!To celebrate our pre-teen we are giving out birthday cakes for THREE WINNERS

participating in NEBNext-inspired crossword puzzle!

1.

1. In which kit fragmentation, dA-tailing, end-repair and cleanup happens in one tube?

2. If bisulfite treatment is too harsh for your whole genome methylation studies, which other option could you choose?

3. You have a blood sample you want to sequence but want to get rid of globin mRNA and rRNA first. Which kit do you choose?

4. Which element makes it possible to make cDNA library out of single cells or very low inputs?

5. Which problem is small RNA library prep kit from NEB battling against?

6. What makes NEB’s adaptor so unique?

7. In which RNA workflow the input amounts range from 10 ng to 1 ug?

8. Which long-range fragment sequencer is supported by NEB reagents?

9. How can you separate molecular duplicates in NEBNext Direct kit?

10. Where in the manual will you find the info on how many standards you have in the E7630, NEBNext® Library Quant Kit for Illumina®?

Let Anniina (anniina.brofeldt@bionordika.fi) know the correct answers to the crossword puzzle by end of December, and you might get a cake delivered to your office!

2.

3.

4.

5.

6.

7.

8.

9.

10.

FREE SAMPLES for testing

All NEBNext products

-10%

Get your answers from our seminar BioNordika and Eurogentec will arrange a free seminar concerning the changing regulation and help around it. The seminar will be held in Helsinki in January with the title “A manufacturing partner for IVDR"

If you want to participate or have any questions, Anniina is happy to help via email or phone.

2 Valid until 31.12.2019 with promo code BN-WINTER.

Let's talk ...about getting the best start

New PhD student, is that you?Congratulations on your excellent decision to start a PhD thesis!

To make the beginning of your journey smoother, Bionordika wants to offer you a new student starter pack when signing up for our free newsletter.

Here are the instructions for ordering:1. Go to: https://www.bionordika.fi/tilaa-uutiskirje 2. Click which techniques interest you (to personalize the newsletter)3. Write to the comment field the following info: You are a new PhD student + your group and PI name4. Give your contact info5. Wait eagerly the new student starter pack to arrive.

IVDR is coming – brace yourself

Ask Anniina for more information!Anniina Brofeldtanniina.brofeldt@bionordika.fiTel. 020 7410 279

What is LDT?

Can I keep my in-house

PCR/qPCR assay?

How much work there is to

keep my in-house assay?

Can someone help me

with that?

I wonder if I have overdone

my gearfor this?

Valid until 31.12.2019 with promo code BN-WINTER 3

Let's talk ...about saving your time

Interested? Let's talk

Anniina Brofeldtanniina.brofeldt@bionordika.fi

Tel. 020 7410 279

Story of Timo: All-round service support

Our offer on epigenetics services:

This is Timo. Timo just got his article back from a reviewer and reviewer wants Timo to add a section about methylation in his article.

Oh no.

Timo has no laboratory work done concerning epigenetics. Timo is sad.

Timo remembers he is not alone. He reaches out to his loyal and incredibly awesome scientific

supplier, BioNordika Oy.

BioNordika Oy informs their supplier Diagenode about the situation Timo has: ”We must help Timo! Quickly!”

Timo’s samples are fetched from him and shipped to the knowledgeable scientists at Diagenode.

PING!

BANG!

BOOM!

Results from Timo’s samples are rushed back to Timo. There is no time to waste when it comes to

almost ready papers!

Timo’s frown is gone when he reads that his paper is approved for publishing.

He now knows that the extensive epigenetics

services from Diagenode will save him in the field

of methylation andchromatin

studies as well as with RNA-seq

if needed!

MethyledDNA

ChromatinRNA

Don’t forget the new EM-seq from NEB, a wonderful addition to our methylation study portfolio!

• ChIP-seq and ChIP-qPCR• ChIPmentation• ATAC-seq• miRNA & small RNA• mRNA• lncRNA

• Reduced representation bisulfite sequencing (RRBS)• Whole genome bisulfite sequencing (WGBS)• Methylated DNA immunoprecipitation (MeDIP)• MagMeDIP• Illumina Infinium EPIC arrays• Targeted methylation studies

I can help you!Hanna Laiho hanna.laiho@bionordika.fi Tel. 020 7410 280

4

CELLEDYRKNING

Valid until 31.12.2019 with promo code BN-WINTER.

Let's talk ...about nucleic acid gel electrophoresis

Do you have a favorite ladder you are using every year? Maybe you remember even the ordering number or the band sizes?

Ladders may not be the most exciting products that you are working with, but you can save money by checking the price and how much product you are using per well. A good ladder is like a loyal friend, the one that never fails you!

We offer DNA, RNA and protein ladders, both prestained and unstained options.

NEB Ladders

Easier and safer tools Protein Ark Agarose tablets and non-carcinogenic DNA stain

End of year sale!

Protein Ark Agarose tablets:• Ready-to-Use tablet format: No messy weighing and improved gel-to-gel reproducibility• Prepare your gel in minutes, all you need is running buffer• Ultrapure Agarose: High resolution of DNA fragment with low background Magnite Green DNA stain:• Safer, non-carcinogenic alternative to Ethidium Bromide• Detect bands under Blue light or UV illuminator

BioNordika Standard Agarose is ideal for identification and recovery of roughly >1000 bp nucleic acid fragments by electrophoresis.

• DNAse and RNAse free• Sharp resolution• Strong gels with low background

-5%on all NEB

ladders

Cat. no. Description OfferPAL-E-2in1-100 Elite 2-in-1 Agarose tablets, 100 pcs 100 €

PAL-E-MGS-100 Magnite Green DNA stain, 1 ml 95 €

Refill your stocks! BioNordika agarose

Cat. no. Description OfferBN-50004 Standard Agarose – Type LE, 500 gram 250 €

5

CELLEDYRKNING

Valid until 31.12.2019 with promo code BN-WINTER.

Let's talk ...about updating your lab gear

Cat. no. Description OfferBN-50004 Standard Agarose – Type LE, 500 gram 250 €

Need new tools for your research? Get a spinner for free! • Is your centrifuge getting tired?• Do you have enough capacity in your shaker?• Do you need another dry bath?

Contact Hanna for more information

Hanna Laiho hanna.laiho@bionordika.fi

Tel. 020 7410 280

siRNA with the new, unique RNAi-cap® technology • Stronger gene knockdown (up to 5-fold)• Reduced off-target effects• Not dependent on the algorithm• Longer gene knockdown (up to 12 days)• Protects from degradation in serum (up to 72h)• Gene knockdown guarantee!• Endotoxin-free CLINIgrade version for in vivo use

iBONi siRNA controls: validated with RNAi-cap®Test the difference! NEW!

Purchase new instruments from our suppliers Labnet International and Cleaver Scientific, worth for >1000 € and get a

spinner centrifuge FREE OF CHARGE!*

Co-Mix Combined Mixer and Vortexer

The only instrument to offer simultaneous microplate mixing and tube vortexing in one compact design

Description The Vitl Co-Mix is the only instrument to offer simultaneous, independently controlled microplate mixing and tube vortexing in one compact design.

The Co-Mix supports 96 and 384 well microplates in skirted, semi-skirted and unskirted variants plus deep-well plates, 0.2ml PCR tubes and PCR strips as well as 0.5, 1.5 and 2.0ml micro test tubes.

The Vortexer supports most skirted and unskirted tube formats.

Benefits

Intuitive touch-screen user interface Effortless changing between operating modes Pre-programmed with commonly used mixing programs User-defined mixing programs can be added to suit your needs Innovative plate-holding mechanism to avoid sample spillage or

contamination when loading and unloading microplates

Accessories

The Co-Mix also comes complete with a full set of adapter plates as standard.

Extra adapter plates can be purchased separately.

Technical Specifications

Dimensions (mm) W x D x H 190 x 350 x 200

Weight 7kg

Speed range (mixer) 200 – 3000 RPM

Speed range (vortexer) 700 – 3500 RPM

Time range (mixer) 5 seconds – 28 days

Time range (vortexer) 2 seconds – 60 seconds

Preset programs (mixer) 9

Preset programs (vortexer) 1

User defined programs Memory for 190 additional

user programs

Power Supply 100 – 240 VAC nominal

Catalogue number

V101001 Vitl Co-Mix (adapter plates included)

Please contact sales@vitlproducts.com for ordering details for the Co-Mix T: +44 1233 638 383 UK

T: +1 804 381 0905 USA

vitlproducts.com *One per order

6

CELLEDYRKNING

Let's talk ...about substrates for IHC

Valid until 31.12.2019 with promo code BN-WINTER.

Get the brightest colors in your IHC with Vector ImmPACT substrates!

Plus IHC kits include:• The normal kit reagents in Ready-To-Use (R.T.U.) format • BLOXALL® endogenous enzyme quenching solution (SP-6000)• ImmPACT® DAB EqV substrate (SK-4103)

Get added convenience with optimized and matched reagents in one product.

Cat. no. Description OfferMP-7800 ImmPRESS® Universal PLUS Polymer Kit, HRP

(Horse Anti-Mouse/Rabbit IgG), 15 ml218 €

MP-7801 ImmPRESS® Horse Anti-Rabbit IgG PLUS Polymer Kit, HRP, 15 ml

218 €

MP-7802 ImmPRESS® Horse Anti-Mouse IgG PLUS Polymer Kit, HRP, 15 ml

218 €

PK-8200 VECTASTAIN® Elite® ABC Universal PLUS Kit, HRP (Horse Anti-Mouse/Rabbit IgG), 50 ml

440 €

Get more and save with the new Plus IHC kits

Questions about IHC?Juho Niva Juho.niva@bionordika.fi Tel. 020 7410 276

...and other ImmPACT Substrates: ImmPACT DAB EqV HRP (Cat# SK-4103), ImmPACT SG HRP (Cat# K-4705), ImmPACT AEC HRP (Cat# SK-4205), ImmPACT AMEC Red HRP (Cat# SK-4285)

Vector Laboratories ImmPACT™ line of substrates offer the following advantages:• 2-4 fold greater sensitivity than the original substrate kits• Supplied with stock solutions in dropper bottles and optimized diluent• Optimized specifically for immunohistochemistry applications• Stock solutions stable for one year, working solution stable for up to 14 days at 4 ºC

All ImmPACT substrates

-14%

ImmPACT DAB HRP #SK-4105

ImmPACT VIP HRP #SK-4605

ImmPACT NovaRED HRP #SK-4805

ImmPACT Vector RED AP#SK-5105

7

CELLEDYRKNING

Let's talk ...about protein purification

Valid until 31.12.2019 with promo code BN-WINTER

Protein Ark’s HiFliQ 1 ml and 5 ml FPLC columns come pre-packed and ready for use with pre-charged Heparin, Nickel-NTA, Cobalt-NTA and Glutathione Agarose resin for affinity purification of Heparin-binding protein, poly-histidine and GST-tagged recombinant proteins.

HiFliQ columns are compatible with all common chromatography instruments (including ÄKTA™ FPLC’s) and a wide range of reducing agents, detergents and other buffer additives.

Pre-packed HiFliQ FPLC Columns

Name Resin Column capasity Units1 ml HiFliQ Ni-NTA FPLC column Ni-NTA 50-75 mg 1 x 1 ml, 5 x 1 ml

5 ml HiFliQ Ni-NTA FPLC column Ni-NTA 250-300 mg 1 x 5 ml, 5 x 5 ml

1 ml HiFliQ GST FPLC column Glutathione 10 mg 1 x 1 ml, 5 x 1 ml

5 ml HiFliQ GST FPLC column Glutathione 50 mg 1 x 5 ml, 5 x 5 ml

1 ml HiFliQ Co-NTA FPLC column Co-NTA 40-50 mg 1 x 1 ml, 5 x 1 ml

5 ml HiFliQ Co-NTA FPLC column Co-NTA 200-250 mg 1 x 5 ml, 5 x 5 ml

1 ml HiFliQ HeparinFPLC column Heparin 3-5 mg 1 x 1 ml, 5 x 1 ml

5 ml HiFliQ HeparinFPLC column Heparin 15-20 mg 1 x 5 ml, 5 x 5 ml

-10%HiFliQ FPLC

Columns

Contact Juho for more information

Juho Niva Juho.niva@bionordika.fi

Tel. 020 7410 276

NEW! HiFliQ Ni Advance FPLC Columns

Simply load your culture media onto the column!HiFliQ Ni Advance FPLC Columns (1 ml & 5 ml) are designed for faster purification of secreted proteins using clarified culture media directly. The Fastback Ni Advance resin resistant to EDTA (up to 20 mM) and DTT (up to 20 mM) for IMAC chromatography.

There is still time to take advantage of Cell Signaling Technology (CST) B3G4 campaign. Choose 4 products from CST’s entire portfolio of primary and secondary antibodies, antibody sampler kits, ELISA kits, SimpleChIP kits and companion products. We’ll give the cheapest item for FREE!Need help choosing? Contact our antibody specialist Juho!

The best antibody deal of the year!

Only valid until 13.12.2019

Endothelial cells are often used to study f.ex. angiogenesis and it’s role in the develop-ment of cancer and metastasis, cardiovascular diseases and blood brain barrier models• Human umbilical vein endothelial cells (HUVEC)• EGMTM Endothelial Cell Growth Media family

Hepatocytes are commonly used in drug development phases but also in the study of non-alcoholic fatty-liver disease• Plateable human hepatocytes• Hepatocyte culture BulletKit, Thawing medium, Plating medium

Neuronal cells are useful for the study of pathogenesis in the neurodegenerative diseases• Especially rodent neurons and glial cells• PNGM Primary Neuron Growth Medium, AGM Astrocyte Growth Medium

Hematopoietic cells can feel like easy-to-obtain but, in fact, can be difficult to isolate and characterize. Wide variety of different hematopoietic cells can be used to study f.ex. immunotherapies and cell therapy.• PBMCs, CD34+ cells• X-Vivo media

Contact Hanna for more information

Hanna Laiho hanna.laiho@bionordika.fi

Tel. 020 7410 280

BioNordika Finland Oy • Kutomotie 18, 00380 Helsinki tel 0207 410 270 • info@bionordika.fi • www.bionordika.fi

Promo code: BN-WINTER19Note! The promotional code must be referenced when ordering to receive the

discounts and special prices. The offers are listed ex. VAT and shipping charges, and are valid until 31.12.2019. The offers cannot be combined with other offers

or agreements. We reserve the right for typos.

CELLEDYRKNING

Let's talk ...about biologically more relevant cell culture

Primary cells do better with optimized set of medium and growth factors

Your benefits:• Massive pool of donors available - get the right tools for your research• Easy to source just when you need the cells• Strict Quality Control and guaranteed performance

20% discount d

when a vial of cells* is purchased together with an optimized Bullet Kit for cultivation!

*Ask for a list of cells included in the campaign