HANDS-ON APPROACH TO CHROMATOGRAPHY Nataša Gros 1 Margareta Vrtačnik 2, M. Filomena Camões 3 1...
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Transcript of HANDS-ON APPROACH TO CHROMATOGRAPHY Nataša Gros 1 Margareta Vrtačnik 2, M. Filomena Camões 3 1...
HANDS-ON APPROACHHANDS-ON APPROACHTO CHROMATOGRAPHYTO CHROMATOGRAPHY
Nataša GrosNataša Gros11 Margareta Vrtačnik Margareta Vrtačnik22, M. Filomena , M. Filomena CamCamõõeses33
1 1 Faculty of Chemistry and Chemical Technology, Faculty of Chemistry and Chemical Technology, 22 Faculty of Natural Sciences and Engineering, Faculty of Natural Sciences and Engineering,
University of Ljubljana, SloveniaUniversity of Ljubljana, Slovenia3 3 University of Lisbon, PortugalUniversity of Lisbon, Portugal
Project No: SI/03/B/F/PP-176012© FKKT, Ljubljana, 2003-2005
NavigationNavigation – – nextnext Navigation Navigation –– back to modules back to modules
ExperimentExperiment
Video clipVideo clip
LEGEND
Next slideNext slide
1. Introduction to chromatography
2. Gas chromatography
3. Liquid chromatography
4. Chromatography –fields of application
Examples of slides
A simplified gas chromatograph
Cylinder with carrier gas
Flow regulator
InjectorColumn with
stationaryphase
Detector
display
Record of
measurement values
-0.005
0
0.005
0.01
0.015
0.02
0.025
0.03
0.035
0 50 100 150 200 250 300 350 400 450 500
t /s
Odz
iv /
V
Chromatogram
Graphic presentation of measurement valuesvs time
Chromatographicpeak of the
component elutedfirst
Chromatographicpeak of the
component elutedsecond
RESPONSE
A simplified liquid chromatograph
Chromatographic system with vacuum water-jet pump
Containerwith
eluentPump
Sample injection
port
Columnwith
stationaryphase
Detector
display
Record of
measurement values
“Separating the dyes from mint aroma
Video: clickon the icon
“Separation of the dyes from mint aroma
Green component
blue component
AcknowledgementsThis Project has been carried out with the support of the
European Community. The content of this project does not necessarily reflect the
position of the European Community, or National Agency.
For this reason neither the National Agency nor the European Community take any responsibility regarding the content of the report.