VENUS II - Freeflight.manual.free.fr/axis-venus2_uk.pdf · Venus II 4 2.
HAMILTON Software VENUS VENUS 96 Head Strategies.
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Transcript of HAMILTON Software VENUS VENUS 96 Head Strategies.
HAMILTON Software VENUS
VENUS96 Head Strategies
2 Strategies
• “Potato printing” – removing some tips then using the remaining ones
• Shifted tip pick up – picking up tips with the head over only part of the rack
HAMILTON Software VENUSUsing the 96 Head to pipette less than 96 samples
HAMILTON Software VENUSPotato Printing
•Create a rack that contains just the tips you want to use
• Pickup some tips with the channels and throw them away
• Pickup tips with the channels and use them (e.g. to add controls) then throw them away
• Pick up tips with the channels and put them in an empty rack
Details
HAMILTON Software VENUSPotato Printing
•Pick up the remaining tips with the 96 head
Sample Tracking
This sequence must include the whole rack of tips
HAMILTON Software VENUSShifted Tip Pickup
There must be space around the rack
For clear tip racks, you will need a tip support (p/n 182040)
Nestable tips should be placed on an NTR1 module (p/n 191420)
HAMILTON Software VENUSShifted Tip Pickup
Ensure the tip sequence is correctly sorted
To pick up columns, sequence sorted in columns
To pick up rows, sequence sorted in rows
Either order will work for single tips
HAMILTON Software VENUSShifted Tip Pickup
First, move the tips to the correct position
Use a CORE 96 Head Tip Pick Up (single step) to pick up the tips from a standard rack and the Tip Eject step to put them in the support
Move your nestable tip rack from a stack to this position (CORE grippers or iSWAP)
HAMILTON Software VENUSShifted Tip Pickup
Picking up one or more columns of tips
Mode is (3) Columns(s)
Select the number of columns here – this is view of the head from above
Ignore this – it is incorrect
HAMILTON Software VENUSShifted Tip Pickup
Picking up one or more columns of tips
The channel pattern in the previous image will result in this pickup
tip rack96 head
Take care that there is nothing here that the 96 head will hit
Multiple Cycles
HAMILTON Software VENUSShifted Tip Pickup
Picking up one or more rows of tips
Tip sequence must be sorted in rows
HAMILTON Software VENUSShifted Tip Pickup
Picking up one tip
Choose which corner of the head