HAMILTON Software VENUS VENUS 96 Head Strategies.

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HAMILTON Software VENUS VENUS 96 Head Strategies

Transcript of HAMILTON Software VENUS VENUS 96 Head Strategies.

Page 1: HAMILTON Software VENUS VENUS 96 Head Strategies.

HAMILTON Software VENUS

VENUS96 Head Strategies

Page 2: HAMILTON Software VENUS VENUS 96 Head Strategies.

2 Strategies

• “Potato printing” – removing some tips then using the remaining ones

• Shifted tip pick up – picking up tips with the head over only part of the rack

HAMILTON Software VENUSUsing the 96 Head to pipette less than 96 samples

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HAMILTON Software VENUSPotato Printing

•Create a rack that contains just the tips you want to use

• Pickup some tips with the channels and throw them away

• Pickup tips with the channels and use them (e.g. to add controls) then throw them away

• Pick up tips with the channels and put them in an empty rack

Details

Page 4: HAMILTON Software VENUS VENUS 96 Head Strategies.

HAMILTON Software VENUSPotato Printing

•Pick up the remaining tips with the 96 head

Sample Tracking

This sequence must include the whole rack of tips

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HAMILTON Software VENUSShifted Tip Pickup

There must be space around the rack

For clear tip racks, you will need a tip support (p/n 182040)

Nestable tips should be placed on an NTR1 module (p/n 191420)

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HAMILTON Software VENUSShifted Tip Pickup

Ensure the tip sequence is correctly sorted

To pick up columns, sequence sorted in columns

To pick up rows, sequence sorted in rows

Either order will work for single tips

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HAMILTON Software VENUSShifted Tip Pickup

First, move the tips to the correct position

Use a CORE 96 Head Tip Pick Up (single step) to pick up the tips from a standard rack and the Tip Eject step to put them in the support

Move your nestable tip rack from a stack to this position (CORE grippers or iSWAP)

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HAMILTON Software VENUSShifted Tip Pickup

Picking up one or more columns of tips

Mode is (3) Columns(s)

Select the number of columns here – this is view of the head from above

Ignore this – it is incorrect

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HAMILTON Software VENUSShifted Tip Pickup

Picking up one or more columns of tips

The channel pattern in the previous image will result in this pickup

tip rack96 head

Take care that there is nothing here that the 96 head will hit

Multiple Cycles

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HAMILTON Software VENUSShifted Tip Pickup

Picking up one or more rows of tips

Tip sequence must be sorted in rows

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HAMILTON Software VENUSShifted Tip Pickup

Picking up one tip

Choose which corner of the head