Hadar Less

Frontiers in Metabolomics

Integration ofIntegration of

Metabolomics Metabolomics & &

TranscriptomicsTranscriptomics

Information FlowInformation Flow

DNA mRNA Protein Reaction

Integration:Integration:

The whole is larger then The whole is larger then the sum of its parts.the sum of its parts.

The combining and coordinating of The combining and coordinating of separate parts or elements into a separate parts or elements into a unified whole.unified whole.

The Holy GrailThe Holy Grail

Identify function of unknown Identify function of unknown genegene

Identify unknown metaboliteIdentify unknown metabolite

Cluster genes & metabolites Cluster genes & metabolites (pathways)(pathways)

Biological DrawbacksBiological Drawbacks

Several genes encode the same Several genes encode the same enzymeenzyme

One enzyme work on different One enzyme work on different substratessubstrates

One substrate participate in One substrate participate in different reactionsdifferent reactions

Technical DrawbacksTechnical Drawbacks

Information gap between mRNA to Information gap between mRNA to reactionsreactions

Measuring snapshots instead of Measuring snapshots instead of dynamicsdynamics

Low variable to observation Low variable to observation ratioratio

Current publicationsCurrent publications

VisionVision

InfrastructureInfrastructure

What can we do?What can we do?

genes compressiongenes compression

G1

Gn

E1

What can we do?What can we do?

Branch point analysisBranch point analysis

M1

M2

M5

M3

M4 M6

M8M7

E12

E25

E68E67E45

E34 E36

Branch point Branch point enzymesenzymes

What can we do?What can we do?

Imitate dynamicsImitate dynamics

ratioratio

timetime

++++++++++

++++

++

What can we do?What can we do?

Construct public datasetsConstruct public datasets

Integration of Integration of Metabolomics Metabolomics

& & TranscriptomicsTranscriptomics

Hadar Less

Frontiers in Metabolomics