h Plc Detectors
-
Upload
mauricio-ronald-cruz-cruz -
Category
Documents
-
view
217 -
download
0
description
Transcript of h Plc Detectors
![Page 1: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/1.jpg)
HPLC Bulk Property Detectors
Refractive Index Detector
![Page 2: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/2.jpg)
Refractive Index Detector
Plus:1) Measures a bulk property2) Nearly Universal (different RI than mobile phase)3) Comparable response for different analytes4) Detects species with no chromophores
Minus:1) Temperature dependent2) Poor sensitivity (LOD ≈ 100 ng)3) No gradient elution
HPLC Bulk Property Detectors
![Page 3: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/3.jpg)
Evaporative Light Scattering Detector
Solid state laser
HPLC Bulk Property Detectors
![Page 4: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/4.jpg)
Plus:1) Measures a bulk property2) Nearly Universal (must be non-volatile)3) Does not detect liquids (gradients are ok)4) Detects species with no chromophores
Minus:1) Signal not linear with concentration2) Fair sensitivity (LOD ≈ 1 ng)3) No salts or buffers in mobile phase
Evaporative Light Scattering Detector
HPLC Bulk Property Detectors
![Page 5: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/5.jpg)
Conductivity Detector
HPLC Bulk Property Detectors
![Page 6: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/6.jpg)
Plus:1) Measures a bulk property2) Nearly Universal (must be ionic)3) Common for Ion Exchange LC4) Detects species with no chromophores5) Simple, robust
Minus:1) Fair sensitivity (LOD ≈ 1 ng)2) No salts or buffers in mobile phase3) Gradients a challenge
HPLC Bulk Property Detectors
Conductivity Detector
![Page 7: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/7.jpg)
HPLC Specific Property Detectors
Conventional UV-Vis Absorption Detector
Hg PenLamp
254 nm
Photodiode 2
Photodiode 1
![Page 8: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/8.jpg)
HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
DeuteriumLamp
PhotodiodeArray
![Page 9: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/9.jpg)
HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
![Page 10: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/10.jpg)
HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
Plus:1) Measures a specific property2) Nearly Universal (must have chromophore)3) Most common of all detectors (~75%)4) Potential to provide qualitative info.5) Simple, robust
Minus:1) Fair sensitivity (LOD ≈ 1 ng)2) Expensive with PDA, limited with Hg Lamp3) Misses some important analytes
![Page 11: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/11.jpg)
HPLC Specific Property Detectors
Fluorescence Detector
![Page 12: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/12.jpg)
HPLC Specific Property Detectors
Fluorescence Detector
![Page 13: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/13.jpg)
![Page 14: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/14.jpg)
HPLC Specific Property Detectors
Plus:1) Measures a specific property2) Highly Selective (must fluoresce)3) Second most common of all detectors (~15%)4) High sensitivity (LOD ≈ 0.01 ng)5) Can interrogate very small volumes
Minus:1) Not Universal2) Limited Applications
Fluorescence Detector
![Page 15: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/15.jpg)
HPLC Specific Property Detectors
Electrochemical Detector
![Page 16: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/16.jpg)
HPLC Specific Property Detectors
Plus:1) Measures a specific property2) Highly Selective (depends on reduction potential)3) High sensitivity (LOD ≈ 0.01 ng)
Minus:1) Not Universal2) Must have electrolyte in mobile phase3) Mobile phase must be aqueous4) Gradients not possible
Electrochemical Detector
![Page 17: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/17.jpg)
HPLC Element Specific Detectors
ICP Detector Detector
![Page 18: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/18.jpg)
Plus:1) Can be Highly Selective2) Can be nearly Universal3) Can have single sensitivity for all analytes
Minus:1) $$$2) Mobile phase must be aqueous4) Mobile phase must not contain element of interest
ICP Detector Detector
HPLC Element Specific Detectors
![Page 19: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/19.jpg)
UV-Vis detection of sugars in juice
![Page 20: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/20.jpg)
UV-Vis detection of sugars in juice
![Page 21: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/21.jpg)
ICP Carbon detection of sugars in juice
![Page 22: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/22.jpg)
ICP Carbon detection of sugars in orange juice
![Page 23: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/23.jpg)
ICP Carbon detection of amino acids
![Page 24: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/24.jpg)
ICP Carbon detection of amino acids
![Page 25: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/25.jpg)
![Page 26: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/26.jpg)
Tag analytes with fluorophoreBefore or After Separation
HPLC Derivatization Detectors
![Page 27: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/27.jpg)
Tag analytes with fluorophoreBefore or After Separation
HPLC Derivatization Detectors
![Page 28: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/28.jpg)
Detect the change in a constant signalas the analyte passes
HPLC Indirect Detectors
![Page 29: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/29.jpg)
Hollow Cathode Detector
Recent HPLC Detector
![Page 30: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/30.jpg)
Hollow Cathode Detector
Recent HPLC Detector
![Page 31: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/31.jpg)
Hollow Cathode Detector
Recent HPLC Detector
![Page 32: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/32.jpg)
Hollow Cathode Detector
Recent HPLC Detector
![Page 33: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/33.jpg)
Hollow Cathode Detector
Recent HPLC Detector
![Page 34: h Plc Detectors](https://reader036.fdocuments.us/reader036/viewer/2022062519/5695d06f1a28ab9b02926f61/html5/thumbnails/34.jpg)
Hollow Cathode Detector
Recent HPLC Detector