Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open...

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Guide to importing images from ImageJ/FIJI to Photoshop and Illustrator 1) Open image in FIJ and split stack into images Dr Andrew Burgess

Transcript of Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open...

Page 1: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

Guide to importing images from ImageJ/FIJI to Photoshop and Illustrator

1) Open image in FIJ and split stack into images

Dr Andrew Burgess

Page 2: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

2) Change LUT to greys (NB: you are not interested in changing colour in PS you can keep colour LUT is you like)

3) Make sure images are RGB Colour

Page 3: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

4) Save your Files as TIFFs

5) Open Photoshop (PS), then from file menu choose scripts>Load Files into Stack…

Page 4: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

6) Choose Browse and locate your TIFF files you just savedand click Open and then OK.

7) This will load all of your images into separate layers

Page 5: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

8) Make 3 new folders by clicking on

the folder icon 3 times

9) Drag each image into a folder Folder Icon

10) You can now ad as many adjustment layers as you like

to each image. Normally Levels and Hue/Saturation are the only ones you will

need.

NB: Adjustment layers are great as they provide non-

destructive editing abilities and allow you to quickly turn off

and on your changes.

Levels and Hue/Saturation

Page 6: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

10) Click on the adjustment layer and a Properties panel should appear. You can use

this to play and alter theimage as you want.

11) To colour an image click the colourize box, then move

Saturation to 100%And Lightness down to -30 to

-40%Move the colour slider to your

desired colour.

Page 7: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

12) NB: currently you will only see the top layer image. To make a composite overlay image select each Folder Group

and then from the dropdown menu select Screen.Repeat for other Group/s

13) You should now have an image that looks like this

Page 8: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

14) To copy all the layers as a single merged image to Adobe Illustrator, first select all (cmd+A)

14) Then choose Copy Merged from Edit menu (or shift+cmd+C).

You can then paste (cmd+V) this Image into your Illustrator File

Page 9: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

15) Optional: If you want to copy each coloured or grey scaled layer individually, just hide the layers your not

interested in. Then repeat Step 14.

Only the green layer now visible

Page 10: Guide to FIJI-Photoshop Image manipulation · ImageJ/FIJI to Photoshop and Illustrator 1) Open imageinFIJ and split stack into images DrAndrew Burgess. 2) Change LUT to greys (NB:

16) For Grey scale image, just hide the Hue/Saturation adjustment layer

Here is anexample of a

finished montageimage in illustrator