Growth and Cell Division
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Transcript of Growth and Cell Division
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Growth and Cell Division
Growth:– Not size,
but number of cells
Bacteria:– Binary
fission Yeast:
– Budding
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Cell Division
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Bacterial Growth Curve
Lag phase
Log phase
Stationary phase
Death phase
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Measuring Bacterial Concentration
Serial dilution/ Standard plate counts
CFU = Colony Forming Unit
CFU/ml
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Measuring Bacterial Growth
Direct microscopic counts
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Measuring Bacterial Growth Most probable
number
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Measuring Bacterial Growth
Filtration
Turbidity (Spectrophotometry)
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Physical Factors Affecting Bacterial Growth
pH Temperature
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Oxygen
Moisture
Physical Factors Affecting Bacterial Growth
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Osmotic pressure– Salt
Radiation
Physical Factors Affecting Bacterial Growth
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Nutritional Factors Affecting Bacterial Growth
Carbon source Nitrogen source Sulfur/ phosphor Trace elements Vitamins Nutritional complexity Enzymes:
– Extracellular– Periplasmic– Cytoplasmic
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Sporulation
Endospores
Other sporelike bacterial structures
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Culturing Bacteria — Streak Plates Streak plate method
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Culturing Bacteria — Pour Plates Pour plate method
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Culture Media Purposes
Selective media
Differential media
Enrichment media
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Culture Conditions — O2
Controlling oxygen content
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Culture conditions
Maintaining cultures
Preserved cultures
Diagnostic tests
Limitations
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