Mitochondria = aerobic respiration

•Intermembrane space= proton (H+) pool

•Mitochondrial matrix = Krebs cycle (Stage 2)

•Christae (sections of inner membrane that protrude inward) = Electron Transport Chain (Stage 3)

*Glycolysis (Stage 1) occurs in the cytoplasm of cell anaerobic

REVISITING MITOCHONDRIA

OVERVIEW OF CELLULAR RESPIRATION

The first step in cell respiration

Occurs in the cytoplasm of the cell

The production of 2 pyruvate molecules from 1 glucose molecule (through a 10-step series of

enzyme-catalyzed reactions)

2 ATP used; 4 ATP produced

GLYCOLYSISWhat is it?

Substrate-level Phosphorylation (pg. 95)

Glycolysis

• Using the induced-fit model, enzymes in glycolysis help phosphate groups hook up

• The enzyme binds 2 substrates- ADP + Pi

ATP ADP + Pi

Direction of reaction depends on stage in the cycles

This occurs at two of the steps of glycolysis

Glycolysis (pg. 98)

1. Glucose glucose-6-phosphate (G6P)

2. G6P fructose-6-phosphate (F6P)3. F6P fructose-1,6-biphosphate (F1,6-BP)

4. F1,6-BP dihydroxyacetone phosphate (DHAP)

5. glyceraldehyde-3-phosphate (G3P)

ATP ADP

ATP ADP

Glycolysis2 molecules of G3P undergo identical series of

reactions:6. G3P 1,3-bisphosphoglycerate (BPG)

7. BPG 3-phosphoglycerate (3PG)

8. 3PG 2-phosphoglycerate (2PG)

NAD+ NADHPi

ADP ATP

Glycolysis9. 2PG phosphoenolpyruvate (PEP)

10. PEP Pyruvate

H2O

ADP ATP

Glucose + 2 ADP + 2 Pi + 2 NAD+ ----> 2 Pyruvate + 2 ATP + 2 (NADH + H+)

GlucoseSong

http://www.northland.cc.mn.us/biology/Biology1111/animations/glycolysis.html

Glycolysis animation