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PANA RealTyper™
· HPV· STD
PANA qPCR™
· EML4-ALK Screening· EML4-ALK Genotyping
PANAMutyper™ R
· EGFR· KRAS· NRAS· BRAF
PNAClamp™
· EGFR· KRAS· NRAS· BRAF· PIK3CA· IDH1· JAK2· BCR-ABL
PNA Oligos
· PNA FISH Telomere Probe· PNA FISH Centromere Probe· miRNA inhibitor· Gamma PNA· Real-time PCR Probe· Globin Redection RNA· Custom PNA
Global Leader of Molecular Diagnostics & Platform Technology Provider
For more information, please visit www.panagene.com 2
PNA is an artificially synthesized nucleic acid.
Phosphate ribose ring of DNA is replaced to polyamide backbone of N-(2-aminoethyl)glycine.
Despite radical structure change, PNA still binds to its complementary DNA or RNA sequences by Watson-Crick base pairing.
PNA provides several physicochemical advantages with stronger binding affinity and chemical stability as probes for molecular diagnostics and antisense drugs.
Criteria PNA DNA
Binding affinity with DNAStronger
(1°C higher per base)
Hybridization rate Faster (100 times)
Salt conc. for hybridization Independent Dependent
ΔTm of single mismatched duplex < 24 °C < 12 °C
Chemical stability Stable Unstable in acid
Water solubilityLimited solubility
: enhanced by linkersSoluble
Probe length for diagnosis 11 - 18 25 - 30
Biological stability Stable to nuclease & protease Enzymatic degradation by nuclease
Thermal stability Excellent Moderate
PNA (Peptide Nucleic Acid)
3|For more information, please visit www.panagene.com 2
PANA qPC
R™
EML4-
ALK
/ PNA O
ligomers
PANA R
ealTyper™PA
NAM
utyper™R Series
PNAC
lamp™
Mutation D
etection Kits
Platform Technologies
Innovative platform technologies in molecular diagnostics area
PNAClamp™
PANA qPCR™ PANA S-melting™
PANA C-melting™ PANArray™
Ct AnalysisMelting-curve
Analysis
selectiveAmplification
MultiplexTarget detection
MultiplexGenotyping
Selective amplification& Multiplex Genotyping Microarray
Somatic Mutation1% detection
Infectious DiseaseDrug Resistance
Infectious DiseasePersonalized Medicine
Somatic Mutation0.01-0.1%
Genotyping &Expression Profiling
EGFR, KRAS, NRAS,
BRAF, IDH1, IDH2,
PIK3CA, JAC2, c-KIT,
BCR-ABL, HER2, ERCC1
TB/NTM,
EML4-ALK,
CRE,
HPV screening
HPV genotyping
STD
RV
CRE
TB/NTM
EGFR, KRAS, NRAS,
BRAF
HPV genotyping, miRNA,
expression, HBV-Drug,
resistant
HCV genotyping
STD,
MTHFR,
CYP450
PNA / Platform
Technologies
Amplification
Mutant DNA
PNA
Foward
Reverse
Infection Disease
Cancer
For more information, please visit www.panagene.com 4
● PANA C-Melting™Technology
● Features
● ProductsProducts Cat. No. Detectable Mutation Size
EGFR PNAR-3001 Exon 18, 19, 20, 21 (47 mutations) 24 tests
KRAS PNAR-1001 Coon 12, 13, 59, 61, 117, 146 (29 mutations) 24 tests
NRAS PNAR-1101 Coon 12, 13, 59, 61, 117, 146 (31 mutations) 24 tests
BRAF PNAR-2001 V600 (5 mutations) 24 tests
• LOD < 0.1% guaranteed
• Excellent specificity
• Multiplex genotyping
• Relative quantification
• Easy and convenient test procedure
• Powerful analyzer
▶ Validated Specimens - Plasma - Serum - Tissue
▶ Compatible Instruments
- Bio-Rad CFX96 - ABI QuantStudio5
PANAMutyper™R Series
Diagnosis of Cancer for Liquid biopsy
marked
-d(R
FU)/
dT
400
300
200
100
0
-100
40 50 60 70
Temperature, Celsius
Melting Peak
Annealing step
Extension step
Wild-type Allele
Wild-type Allele
Clamping PNA
3'
3'5'
Detection probe
Detection probe
Mutant Allele
Mutant Allele
Mutant Allele
Denaturation
a) b)PCR clamping
MT 10% MT 1%MT 0.1%MT 0.01%MT 0.005%MT 0.001%MT 0%
L858R
5|For more information, please visit www.panagene.com 4
● Advantage of PANAMutyper™
New Advanced Mutation Detection Solution for Circulating Tumor DNA
* *
EGFR T790M EGFR L858R KRAS G12D KRAS G12V KRAS Q61R KRAS Q61K
B Tech N Tech d Tech c Tech Q Tech PANAMutyper™ PNAClamp™
5%
2%1%
0.10%
0.01%
Comparison of somatic mutation detection methods
*:Not detected in 5% mutation samples(false negative), §: No test for those mutations
Comparison of somatic mutation detection methods Superiority of PANAMutyper™: The Choice is Clear
PANAM
utyper™R Series
B Tech d Tech c Tech
N Tech Q Tech
Easiness
LOD
Run time Equipment independancy
Assay cost
PANAMutyper™
c Tech
d Tech
B Tech
Increasing Cost Effectiveness
0.01
0.1
1
5Incr
easi
ng T
est Sen
stiv
ity(%
)(M
edia
n L
OD)
General Info : [email protected]
PANA qPC
R™
EML4-
ALK
/ PNA O
ligomers
PANA R
ealTyper™PN
AC
lamp™
Mutation D
etection Kits
PNA / Platform
Technologies
For more information, please visit www.panagene.com 6
PNAClamp™ Technology is the PNA-based PCR clamping that selectively amplifies only the mutated target DNA sequence as a minor portion in the mixture with the major wild type DNA sequences.
• Compatible with the most popular real-time PCR instruments• Test several biomarkers in one plate - eg.) EGFR+KRAS+BRAF … in one plate• High sensitivity and specificity• Various compatible samples• Mutation detectable with small amount of DNA
Criteria PANAGENE Company A Company B
TechnologyPNAClamp™
& Real-time PCRScorpions™Real-time PCR
& ARMS™Direct sequencing(D-Sequencing)
Sensitivity <1% <1% 15~25%
Procedure Time(except DNA extraction)
2.5 hrs. 3hrs. 11hrs.
Simplicity ++++ ++ +
Reproducibility +++ ++ ++
PNAClamp™Mutation Detection KitsKFDA approved
marked
Real-time PCR-based Ready-to-use Kit● PNAClamp™Technology
● Features
● Panagene’s product vs. other products
PNA annealing
PNA Probe
NO Amplicon
Amplicon
PCR reaction
7|For more information, please visit www.panagene.com 6
▶ Validated Specimens
- Tissue (Fresh, FFPE) - Biopsy
- Pleural effusions
- Cytology samples
▶ Compatible Instruments
- Bio-Rad CFX96
- Roche LC480II
- ABI 7500, 7900, StepOnePlus
- Qiagen Rotor-Gene Q
● Products
* Research Use Only
Products Cat. No. Detectable Mutations Size
EGFR PNAC-3002 Exon 18, 19, 20, 21 (40 mutations) 25 tests
KRAS PNAC-1006 Codon 12, 13, 59, 61, 117, 146 (40 Mutations) 25 tests
NRAS PNAC-1101 Codon 12, 13, 59, 61, 117, 146 (43 Mutations) 25 tests
BRAF PNAC-2001 V600 (5 mutations) 50 tests
PIK3CA PNAC-4001 Codon 542, 545 or 546, 1047 (13 mutations) 25 tests
IDH1 PNAC-5001 R132 (5 mutations) 25 tests
JAK2 PNAC-6001 V617F 25 tests
BCR-ABL* PNAC-7001 T315I 25 tests
Technical Question : [email protected]
PNAC
lamp™
Mutation D
etection Kits
PANA qPC
R™
EML4-
ALK
/ PNA O
ligomers
PANA R
ealTyper™PA
NAM
utyper™R Series
PNA / Platform
Technologies
For more information, please visit www.panagene.com 8
• Tm analysis for high multiplexing in real - time• Specific PNA probe for target HPV, STD and RV
Products Cat. No. Detectable Mutation Size
HPV PNAM-1001 40 HPV types (genotyping 22 types, screening 18 types) 48 tests
STD PNAM-2001 13 STD types (NG, CT, UU, UP, TV, MG, MH, CA, HD, T.P, HSV1, HSV2, GV) 48 tests
PANA RealTyper™marked
● PANA S-Melting™Technology
● Features• High sensitivity and specificity• High reproducibility• Multiplex genotyping
• Easy and convenient test procedure• Powerful analyzer
● Products
DenaturationExtension
Annealing
PNA Probe
PNA Probe
-d(R
FU)/
dT
500
400
300
200
100
0
40 50 60 70 80 90
Temperature, Celsius
Melting Peak
-d(R
FU)/
dT
1200
1000
800
600
400
200
040 50 60 70 80 90
Temperature, Celsius
Melting Peak
-d(R
FU)/
dT
500
400
300
200
100
040 50 60 70 80 90
Temperature, Celsius
Melting Peak
-d(R
FU)/
dT
1000
800
600
400
200
040 50 60 70 80 90
Temperature, Celsius
Melting Peak
PNA Probe(HEX)
PNA Probe(FAM)
PNA Probe(ROX)
PNA Probe(Cy5)
▶ Validated Specimens
- Cervical swab sample - Liquid based cytology sample
▶ Compatible Instruments
- Bio-Rad CFX96 - ABI QuantStudio5
PCR Amplification Melting Curve
9|For more information, please visit www.panagene.com 8
◆ Tube A : NG, CT, UU, UP, TV, MG, MH◆ Tube B : CA, HD, T.P, HSV1, HSV2, GV
Real-time PCR based Multiplex Genotyping Kit
● PANA RealTyper™HPV
◆ Tube A and B : High Risk Type and Low Risk Type - 20 High Risk HPV Genotypes:
16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 70, 73, 82- 2 Low Risk HPV Genotypes:
6, 11
◆ Tube O : Other Types : Screening 18 Types- 18 HPV types: 32, 34, 40, 42, 43, 44, 54, 55, 62, 81, 83, 30, 61,67, 74, 84, 87, 90
● Comparison with Current HPV Testing Methods
● PANA RealTyper™STDPANA RealTyper™ STD Genotyping Kit takes advantages of superior PNA properties to DNA. STD genotyping can be completed in a shorter time with higher sensitivity and specificity. Single or multiple infections among 13 STD can be accurately identified.
UP+MG TV+MG
-d(R
FU)/d
T
400
300
200
100
0
40 50 60 70 80
Temperature, Celsius
Melting Peak
UP+TV+MG
-d(R
FU)/d
T
400
300
200
100
0
40 50 60 70 80
Temperature, Celsius
Melting Peak
-d(R
FU)/d
T
500
400
300
200
100
0
40 50 60 70 80
Temperature, Celsius
Melting Peak
Testing Method Provided Information
Genotyping Co-infection Turn around time
Hybrid capture II X X 3 hours
Microarray /bead ○ ○ Over 5 hours
DNA sequencing ○ X Over 8 hours
Real-time PCR ▲ ▲ 3 hours
PANA RealTyper™ ○ ○ 3 hours
○ : Provided / ▲ Limited provided / X : Not provided
Quote Request : [email protected]
PNAC
lamp™
Mutation D
etection Kits
PANA qPC
R™
EML4-
ALK
/ PNA O
ligomers
PANA R
ealTyper™PA
NAM
utyper™R Series
PNA / Platform
Technologies
For more information, please visit www.panagene.com 10
PANA qPCR™ technology uses a sequence-specific PNA probe with a fluorescent reporter and quencher. The PNA probe is designed to hybridize specifically to a nucleotide region of the target sequence. PNA probe is not hydrolyzed during amplification because its resistant to enzyme degradation. PANA qPCR™ is a powerful tool for multiplexing, allelic discrimination experiments and quantitative analysis with high specificity and sensitivity.
Products Cat. No. Detectable Variants Size
Screening PNAQ-3101 25 variants (V1, V6, V3a, V3b, V2, V4, V7, Vnew, V5a, V5b, V8a, V8b, ect) 24 tests
Genotyping PNAQ-3201 12 variants (V1, V6, V3a, V3b, V2, V4, V7, Vnew, V5a, V5b, V8a, V8b) 10 tests
PANA qPCR™EML4-ALK Screening Kit PANA qPCR™EML4-ALK Genotyping Kit
Components 1 tube ( EML4-ALK fusion gene + Internal control )
4 tube (12 EML4-ALK fusion gene + Internal control )
Coverage 25 EML4-ALK fusion variants detectable 12 EML4-ALK fusion variants detectableDye ROX, HEX ROX, HEX, FAM, CY5
Probe Common Probe Specific ProbeSensitivity 10^2 copy 10^2 copy
Assessment EML4-ALK positive or negative Assess each variantSize 24 tests / kit 10 tests / kit
PANA qPCR™ EML4-ALK™RUO (Research Use Only)
● PANA qPCR™Technology
● Products
● Features
▶ Validated Specimens - Tissue (Fresh, FFPE)- Biopsy- Pleural essusions- Cytology samples
▶ Compatible Instruments
- Bio-Rad CFX96- Roche LL480Ⅱ- ABI7500, 7900, StepOnePlus- Qiagen Rotor-GeneQ
Common probes
Specific probes
Quenched status
Hybridized status
EML4 ALK
F : fluorophoreQ : quencher
F
F
Q
Q
template DNA
Screening
Genotyping
PNA probe
11|For more information, please visit www.panagene.com 10
PNA-DNA hybridization is much faster than DNA-DNA and has shown much higher signal to background noise ratio.
PANAGENE has developed PNA based miRNA Inhibitors with higher sensitivity, reproducibility and stability in the cell. We provide all types of PNAs™ miRNA inhibitors to inhibit miRNA available from miRBase Sequence Database hosted by the Sanger Institute (http://microrna.sanger.ac.uk).
Reference1.Su Young Oh et al., Oligonucleotides. 2010 Oct; 20(5):225-30 2. Fabani MM et al., Nucleic Acids Res 1-10.
Panagene has developed PNA based miRNA Inhibitors with higher sensitivity,reproducibility and stability in the cell. We provide all types of PNAs™ miRNA inhibitors to inhibit miRNAs available from miRBase Sequence Database hosted by the Sanger Institute (http://microrna.sanger.ac.uk).
For more information, please visit www.panagene.com
Telomere probe Centromere probe
- Telomere, Centromere probes- Q-FISH, CO-FISH, Flow-FISH - Fast hybridization- Custom probe design possible
- Unlabeled PNA - Labeled PNAs with dyes, biotin or other functional groups- Modified PNAs for your various research - Special PNAs from unnatural bases - High purity (up to 99%) - Large scale manufacturing - PNA monomers- Bis-PNAs- Gamma PNAs(γPNA)
* right-handed helix* enhanced PNA-DNA stability* more handle for labeling* improved solubility* stereogenic center
PNA Oligomers
● FISH probes
● miRNA Inhibitors
● Custom PNA Oligomers
PNAC
lamp™
Mutation D
etection Kits
PANA qPC
R™
EML4-
ALK
/ PNA O
ligomers
PANA R
ealTyper™PA
NAM
utyper™R Series
PNA / Platform
Technologies
Products List
Products Cat. No. Details Size
PANAMutyper™ R Series (for Plasma)
EGFR PNAR-3001 Exon 18, 19, 20, 21 (47 mutations) 24 tests
KRAS PNAR-1001 Coon 12, 13, 59, 61, 117, 146 (29 mutations) 24 tests
NRAS PNAR-1101 Coon 12, 13, 59, 61, 117, 146 (31 mutations) 24 tests
BRAF PNAR-2001 V600 (5 mutations) 24 tests
PNAClamp™ Mutation Detection Kit (for Tissue)
EGFR PNAC-3002 Exon 18, 19, 20, 21 (40 mutations) 25 tests
KRAS PNAC-1006 Codon 12, 13, 59, 61. 117, 146 (40 mutations) 25 tests
NRAS PNAC-1101 Codon 12, 13, 59, 61. 117, 146 (43 mutations) 25 tests
BRAF PNAC-2001 V600 (5 mutations) 50 tests
PIK3CA PNAC-4001 Codon 542, 545 or 546, 1047 (13 mutations) 25 tests
IDH1 PNAC-5001 R132 (5 mutations) 25 tests
JAK2 PNAC-6001 V617F 25 tests
BCR-ABL PNAC-7001 T315I 25 tests
PANA qPCR™ EML4-ALK Detection Kit
Screening PNAQ-310125 variants
(V1, V6, V3a, V3b, V2, V4, V7, Vnew, V5a, V5b, V8a, V8b, ect.)24 tests
Genotyping PNAQ-320112 variants
(V1, V6, V3a, V3b, V2, V4, V7, Vnew, V5a, V5b, V8a, V8b)10 tests
PANA RealTyper™Kit
Products Cat. No. Details Size
HPV PNAM-1001 40 HPV types (genotyping 22 types, screening 18 types) 48 tests
STD PNAM-200113 STD types
(NG, CT, UU, UP, TV, MG, MH, CA, HD, T.P, HSV1, HSV2, GV)48 tests
Cancer
Infectious Disease
Distributed by Eurogentec Website : www.eurogentec.com Contact: [email protected]