gh Sensitivity DNA Assay DE34903050 2019-09-23 09-16 ... - …

Instrument Name: G2938CDE34903050 Firmware:Serial#:Assay Information:

C.01.069DE34903050

Instrument Information:

Assay Origin Path: C:\Program Files\Agilent\2100 bioanalyzer\2100expert\assays\dsDNA\High Sensitivity DNA.xsy

Assay Class:Version:Assay Comments:

High Sensitivity DNA Assay1.03Copyright © 2003-2010 Agilent Technologies

Chip Information:

Chip Comments:

Type: G2938C

Chip Lot #:Reagent Kit Lot #:

Kursus 2019 library 41 Kursus 2019 library 42 Kursus 2019 library 43

Kursus 2019 library 6 Kursus 2019 library 11

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 9/23/2019 10:25:31 AM

2100 expert_High Sensitivity DNA Assay_DE34903050_2019-09-23_09-16-16.xad Page of1 12

Created:Modified:

9/23/2019 9:16:16 AM9/23/2019 9:40:27 AMData Path:

High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE34903050_2019-09-23_09-16-16.xad

Assay Class:

Electrophoresis File Run Summary

Sample Name Sample Comment Rest. Digest Status Observation Result Label ResultColor

Kursus 2019 library 41Kursus 2019 library 42Kursus 2019 library 43Kursus 2019 library 6Kursus 2019 library 11sample 6sample 7sample 8sample 9sample 10sample 11Ladder

Chip Lot # Reagent Kit Lot #

Chip Comments :



Created:Modified:

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Assay Class:

Electrophoresis File Run Summary (Chip Summary)

General Analysis SettingsNumber of Available Sample and Ladder Wells (Max.) : 12Minimum Visible Range [s] : 32Maximum Visible Range [s] : 138Start Analysis Time Range [s] : 33End Analysis Time Range [s] : 137.5Ladder Concentration [pg/µl] : 1950Uses Standard Area for Ladder FragmentsLower Marker Concentration [pg/µl] : 125Upper Marker Concentration [pg/µl] : 75Used Upper Marker for QuantitationStandard Curve Fit is Point to PointShow Data Aligned to Lower and Upper Marker

Integrator SettingsIntegration Start Time [s] : 33.05Integration End Time [s] : 137Slope Threshold : 0.8Height Threshold [FU] : 5Area Threshold : 0.1Width Threshold [s] : 0.6Baseline Plateau [s] : 0.5

Filter SettingsFilter Width [s] : 0.5Polynomial Order : 4

Ladder Ladder Peak Size Area1 35 1602 50 2103 100 2084 150 2215 200 2426 300 2707 400 3058 500 3069 600 33610 700 32111 1000 36612 2000 41313 3000 41114 7000 40015 10380 214



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Assay Class:

Electrophoresis Assay Details

Peak table for LadderPeak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 50 150.00 4,545.5 Ladder Peak3 100 150.00 2,272.7 Ladder Peak4 150 150.00 1,515.2 Ladder Peak5 200 150.00 1,136.4 Ladder Peak6 300 150.00 757.6 Ladder Peak7 400 150.00 568.2 Ladder Peak8 500 150.00 454.5 Ladder Peak9 600 150.00 378.8 Ladder Peak10 700 150.00 324.7 Ladder Peak11 1,000 150.00 227.3 Ladder Peak12 2,000 150.00 113.6 Ladder Peak13 3,000 150.00 75.8 Ladder Peak14 7,000 150.00 32.5 Ladder Peak15 10,380 75.00 10.9 Upper Marker

Ladder

Overall Results for LadderNoise: 0.2



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Assay Class:

Electropherogram Summary

Peak table for sample 1 : Kursus 2019 library 41Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 330 8.40 38.53 341 4.98 22.14 351 5.16 22.35 372 14.89 60.76 383 11.34 44.97 403 8.74 32.98 428 8.76 31.09 447 7.10 24.110 488 6.27 19.511 10,380 75.00 10.9 Upper Marker12 12,411 0.00 0.0Region table for sample 1 : Kursus 2019 library 41From[bp]

To[bp]

Corr.Area

% ofTotal

Average Size[bp]

Size distribution inCV [%]

Conc.[pg/µl]

Molarity[pmol/l]

Color

200 1,000 224.5 88 425 25.5 180.34 701.4

Kursus 2019 library 41

Overall Results for sample 1 : Kursus 2019 library 41Number of peaks found: 10 Noise: 0.2

Corr. Area 1: 224.5



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Assay Class:

Electropherogram Summary Continued ...

Peak table for sample 2 : Kursus 2019 library 42Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 334 21.35 96.93 357 15.78 67.04 377 11.03 44.35 426 11.56 41.16 501 9.07 27.47 534 10.91 30.98 566 6.60 17.79 588 7.78 20.110 10,380 75.00 10.9 Upper MarkerRegion table for sample 2 : Kursus 2019 library 42From[bp]

To[bp]

Corr.Area

% ofTotal

Average Size[bp]


Conc.[pg/µl]

Molarity[pmol/l]

Color

200 1,000 376.6 98 449 25.4 235.39 867.9



Corr. Area 1: 376.6



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Assay Class:


Peak table for sample 3 : Kursus 2019 library 43Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 439 529.26 1,828.63 515 371.87 1,093.74 561 504.55 1,362.65 3,407 6.25 2.86 10,380 75.00 10.9 Upper Marker7 12,814 0.00 0.0Region table for sample 3 : Kursus 2019 library 43From[bp]

To[bp]

Corr.Area

% ofTotal

Average Size[bp]


Conc.[pg/µl]

Molarity[pmol/l]

Color

200 1,000 2,189.1 97 509 26.8 1,660.39 5,504.0



Corr. Area 1: 2,189.1



Created:Modified:

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Assay Class:


Peak table for sample 4 : Kursus 2019 library 6Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 126 102.71 1,238.03 385 592.60 2,332.54 394 623.96 2,396.85 2,472 85.57 52.56 10,380 75.00 10.9 Upper Marker7 13,302 0.00 0.0Region table for sample 4 : Kursus 2019 library 6From[bp]

To[bp]

Corr.Area

% ofTotal

Average Size[bp]


Conc.[pg/µl]

Molarity[pmol/l]

Color

200 1,000 1,906.2 86 401 15.9 1,259.40 4,918.8



Corr. Area 1: 1,906.2



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Assay Class:


Peak table for sample 5 : Kursus 2019 library 11Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 126 85.90 1,035.23 380 96.49 384.64 418 35.50 128.75 10,380 75.00 10.9 Upper Marker6 13,249 0.00 0.0Region table for sample 5 : Kursus 2019 library 11From[bp]

To[bp]

Corr.Area

% ofTotal

Average Size[bp]


Conc.[pg/µl]

Molarity[pmol/l]

Color

200 1,000 381.9 77 398 19.2 250.12 1,000.4



Corr. Area 1: 381.9



Created:Modified:

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Assay Class:




Created:Modified:

9/23/2019 9:16:16 AM9/23/2019 9:40:27 AMData Path:


Assay Class:

Gel Image

Sample 6 has not been run, no results available.



Created:Modified:

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Assay Class:

Invalid Samples

Description Number Source Category Sub Category Time Time Zone User HostRun ended onport 2 (Numberof wellsacquired: 6)

Instrument Run 9/23/20199:40:24 AM

(GMT +02:00)RomanceStandard Time

Lab W-LAB02

Run started onport 2 (File:C:\ProgramFiles\Agilent\2100bioanalyzer\2100expert\Data\2019-09-23\2100expert_HighSensitivity DNAAssay_DE34903050_2019-09-23_09-16-16.xad)



Lab W-LAB02

ProductNumber :G2938C



Lab W-LAB02

Name :G2938CDE34903050



Lab W-LAB02

Vendor :AgilentTechnologies



Lab W-LAB02

Serial# :DE34903050



Lab W-LAB02

Firmware :C.01.069



Lab W-LAB02

Cartridge :Electrode



Lab W-LAB02



Created:Modified:

9/23/2019 9:16:16 AM9/23/2019 9:40:27 AMData Path:


Assay Class:

Run Logbook

gh Sensitivity DNA Assay DE34903050 2019-09-23 09-16 ... - …

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