Kinds Of Complexity Worst-case complexity. Average complexity. Amortized complexity.
Genomic Complexity
description
Transcript of Genomic Complexity
![Page 1: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/1.jpg)
Genomic Complexity
![Page 2: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/2.jpg)
How many genes in the chromosome?• DNA or RNA?• Double Stranded or Single Stranded?• Shape?• Number of copies?• Size in base pairs?• Assume 400 amino acids per protein, then how many bases
per gene for a typical protein?• How many genes possible?• Do genes overlap?• Are both strands transcribed?
![Page 3: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/3.jpg)
Consider the Ribosome
![Page 4: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/4.jpg)
And its operon
![Page 5: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/5.jpg)
Studying Macromolecules –Extracting DNA
Cell Pellet
Random fragments ~20-40 kbp
![Page 6: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/6.jpg)
Extraction of Chromosomal DNA
• DNeasy Tissue Kit• Chromosomal
DNA, ~ 20-40 kbp size fragments
• Kim Foster
Chromosomal DNA
Chromosomal DNA
RNA
RNA
23 kbp
![Page 7: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/7.jpg)
Hybridization
![Page 8: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/8.jpg)
Quantitative Hybridization
![Page 9: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/9.jpg)
Liquid Scintillation Counters
Photomultiplier Tubes
log Scale
Electronic Noise
3H 14C 32P
Spike
![Page 10: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/10.jpg)
Experiment 1 – Total 14C-Uracil
10 µg DNA
10 µg RNA
5 µg RNA washes off
5 µg RNA sticks
![Page 11: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/11.jpg)
Experiment 2 – rRNA 14C-Uracil
10 µg DNA
10 µg rRNA
>99% rRNA washes off
35 ng rRNA sticks
![Page 12: Genomic Complexity](https://reader035.fdocuments.us/reader035/viewer/2022062814/5681681e550346895dddadf3/html5/thumbnails/12.jpg)
Experiment 3 – tRNA 14C-Uracil
10 µg DNA
10 µg tRNA
>99% tRNA does not stick
5 ng tRNA sticks