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Genome wide selection for Eucalyptus improvement at international paper in Brazil
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POSTER PRESENTATION Open Access Genome wide selection for Eucalyptus improvement at international paper in Brazil Carla Garcia 1* , Bruno Lima 2 , Adriano Almeida 3 , Wesley Marques 4 , Marcos Resende 5 , Roland Vencovsky 6 , Dario Grattapaglia 7 From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery Arraial d’Ajuda, Bahia, Brazil. 26 June - 2 July 2011 Background The efficiency of plant breeding depends mainly on two actions of the breeder: creation and subsequent identifi- cation of superior genotypes. In both actions, selection plays a fundamental role in the definition of crosses to be performed, with interest in creating new genotypes and identifying superior trees to be used commercially. The great attraction of molecular tools for plant breed- ing is the direct use of DNA information in selection, allowing higher efficiency, quickness in obtaining genetic gains with relatively low costs, when compared to the traditional selection based on phenotypic data. All these objectives can be reached through a new approach: gen- ome-wide selection (GWS) or just genomic selection (GS). Genomic selection can be applied in breeding pro- grams of any species. Results obtained in simulated data indicated that GWS can be very profitable in eucalyptus breeding [1]. GWS is based on selection exclusively by molecular markers, after having their genetic effects esti- mated based on phenotypic data, in a breeding popula- tion sample [2]. The present work aims to characterize and estimate genetic parameters of a hybrid progeny test, the population that will compose the genome-wide selection study at International Paper in Brazil. Material and methods The International Paper Brazil population chosen for genome-wide selection purposes is installed in a Hybrid Progeny Test, comprising 58 crosses from controlled pollination of Eucalyptus grandis and Eucalyptus uro- phylla and five common checks (commercial clones of the company), totaling 63 treatments. These 58 families are derived from 56 different parents crossed. The test was installed in July 2006 in Brotas (São Paulo State, Brazil) in randomized complete block design, with six plants per plot and eight blocks, corresponding to a total of 3,024 plants. In 2011 the test was evaluated, per- forming the measurement of diameter at breast high (DBH) and plant height of the progenies. The obtained data were analyzed in Selegen-REML/BLUP software for the estimation of genetic parameters. The analysis, will allow the identification of the elite individual trees, which comprised the GWS population, ranking the best living trees of the test by estimating the annual average increment and morphological aspects (removing dead, forked and broken trees). Following the selection of the GWS 1,000 trees population, xylem samples were col- lected and sent for DNA extraction and genotyping with DArT and SNP markers. Results Three variables were analyzed to characterize the test population: DBH, plant height and wood volume (calcu- lated as function of plant diameter and height). DBH had general mean of 13.8 cm and genotypic variance between progenies of 1.61 cm². The individual narrow- sense heritability of the character was 0.255 ± 0.039, very similar to the plant height heritability of 0.236 ± 0.037. Plant height presented general mean of 21.3 meters and genotypic variance of 2.17 m². The esti- mated wood volume had general mean of 0.168 m3 per plant and genotypic variance of 0.0012 (m3)². The individual narrow-sense heritability was slightly higher than for DBH and plant height, namely 0.297 ± 0.042. All estimates were typical of quantitative traits con- trolled by many loci of small effects. Based on the analy- sis of the experiment, the best trees were selected, as potential clones and parents for crossing. This analysis revealed 44 crosses with elite clones, to be used as * Correspondence: [email protected] 1 International Paper in Brazil, Mogi Guaçu, SP, Brazil Full list of author information is available at the end of the article Garcia et al. BMC Proceedings 2011, 5(Suppl 7):P44 http://www.biomedcentral.com/1753-6561/5/S7/P44 © 2011 Garcia et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Transcript of Genome wide selection for Eucalyptus improvement at international paper in Brazil
POSTER PRESENTATION Open Access
Genome wide selection for Eucalyptusimprovement at international paper in BrazilCarla Garcia1*, Bruno Lima2, Adriano Almeida3, Wesley Marques4, Marcos Resende5, Roland Vencovsky6,Dario Grattapaglia7
From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and DeliveryArraial d’Ajuda, Bahia, Brazil. 26 June - 2 July 2011
BackgroundThe efficiency of plant breeding depends mainly on twoactions of the breeder: creation and subsequent identifi-cation of superior genotypes. In both actions, selectionplays a fundamental role in the definition of crosses tobe performed, with interest in creating new genotypesand identifying superior trees to be used commercially.The great attraction of molecular tools for plant breed-ing is the direct use of DNA information in selection,allowing higher efficiency, quickness in obtaining geneticgains with relatively low costs, when compared to thetraditional selection based on phenotypic data. All theseobjectives can be reached through a new approach: gen-ome-wide selection (GWS) or just genomic selection(GS). Genomic selection can be applied in breeding pro-grams of any species. Results obtained in simulated dataindicated that GWS can be very profitable in eucalyptusbreeding [1]. GWS is based on selection exclusively bymolecular markers, after having their genetic effects esti-mated based on phenotypic data, in a breeding popula-tion sample [2]. The present work aims to characterizeand estimate genetic parameters of a hybrid progenytest, the population that will compose the genome-wideselection study at International Paper in Brazil.
Material and methodsThe International Paper Brazil population chosen forgenome-wide selection purposes is installed in a HybridProgeny Test, comprising 58 crosses from controlledpollination of Eucalyptus grandis and Eucalyptus uro-phylla and five common checks (commercial clones ofthe company), totaling 63 treatments. These 58 familiesare derived from 56 different parents crossed. The test
was installed in July 2006 in Brotas (São Paulo State,Brazil) in randomized complete block design, with sixplants per plot and eight blocks, corresponding to atotal of 3,024 plants. In 2011 the test was evaluated, per-forming the measurement of diameter at breast high(DBH) and plant height of the progenies. The obtaineddata were analyzed in Selegen-REML/BLUP software forthe estimation of genetic parameters. The analysis, willallow the identification of the elite individual trees,which comprised the GWS population, ranking the bestliving trees of the test by estimating the annual averageincrement and morphological aspects (removing dead,forked and broken trees). Following the selection of theGWS 1,000 trees population, xylem samples were col-lected and sent for DNA extraction and genotyping withDArT and SNP markers.
ResultsThree variables were analyzed to characterize the testpopulation: DBH, plant height and wood volume (calcu-lated as function of plant diameter and height). DBHhad general mean of 13.8 cm and genotypic variancebetween progenies of 1.61 cm². The individual narrow-sense heritability of the character was 0.255 ± 0.039,very similar to the plant height heritability of 0.236 ±0.037. Plant height presented general mean of 21.3meters and genotypic variance of 2.17 m². The esti-mated wood volume had general mean of 0.168 m3 perplant and genotypic variance of 0.0012 (m3)². Theindividual narrow-sense heritability was slightly higherthan for DBH and plant height, namely 0.297 ± 0.042.All estimates were typical of quantitative traits con-trolled by many loci of small effects. Based on the analy-sis of the experiment, the best trees were selected, aspotential clones and parents for crossing. This analysisrevealed 44 crosses with elite clones, to be used as
* Correspondence: [email protected] Paper in Brazil, Mogi Guaçu, SP, BrazilFull list of author information is available at the end of the article
Garcia et al. BMC Proceedings 2011, 5(Suppl 7):P44http://www.biomedcentral.com/1753-6561/5/S7/P44
© 2011 Garcia et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.
training/discovery population for GWS purposes. Thesuperior individuals of these crosses were selected tocomplete 1,000 trees, dispersed through the entire test.The number of crosses (44) selected and the differentparents identified (45) are important informations forestimating easily the effective population size (Ne), animportant factor in GWS [1].
ConclusionsThe information and data generated will lead the studiesof the next steps into GWS approach. With the deepunderstanding of the training population that we have,the genomic breeding values (GBV) can be estimatedand the GWS applied using phenotypic and marker datasimultaneously. A correct choice of a breeding popula-tion is essential for traditional plant breeding and is notdifferent in integrated molecular approaches. Subse-quently, the wood phenotyping will be performed andthe GBV also estimated for wood quality traits. Thesedata will start up the implementation of GWS in Inter-national Paper breeding program in Brazil, as opera-tional procedure, looking at quick selection and,consequently, cost decreases.Financial support. International Paper Brazil,
FAPESP, Embrapa-Cenargen
Author details1International Paper in Brazil, Mogi Guaçu, SP, Brazil. 2Department ofGenetics, University of São Paulo - ESALQ/USP, Piracicaba, SP, Brazil.3International Paper in Brazil. 4University of Campinas, Campinas, SP, Brazil.5EMBRAPA Forestry/UFViçosa, Brazil. 6Department of Genetics, University ofSão Paulo - ESALQ/USP, Piracicaba, Brazil. 7EMBRAPA Genetic Resources andBiotechnology, EPqB 70770-910, Brazilia, DF, Brazil.
Published: 13 September 2011
References1. Grattapaglia D, Resende MDV: Genomic selection in forest tree breeding.
Tree Genetics & Genomes 2011, 7:241-255.2. Resende MDV, Lopes PS, Silva RL, Pires IE: Seleção Genômica Ampla e
maximização da eficiência do melhoramento genético. Pesquisa FlorestalBrazileira 2008, 56:63-77.
doi:10.1186/1753-6561-5-S7-P44Cite this article as: Garcia et al.: Genome wide selection for Eucalyptusimprovement at international paper in Brazil. BMC Proceedings 2011 5(Suppl 7):P44.
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Garcia et al. BMC Proceedings 2011, 5(Suppl 7):P44http://www.biomedcentral.com/1753-6561/5/S7/P44
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