Genetic EngineeringGenetic engineering involves the manipulation

of organism genes.

The central tool is the recombinant DNA technique.

- to isolate a gene of interest from one organism

- to insert the isolated gene to a DNA molecule (known as vector) that can replicate.

- to provide an environment (known as recipient cells) that allows reproduction of the joined DNA molecule and generate the gene product.

Genetic Engineering

To transfer a gene from one organism to another

- transformation: plasmid as vector

- transduction: viruses as vector

- conjugation: direct cell-to-cell contact is required.The gene is transferred by an episome (DNA

molecule).

http://en.wikipedia.org/wiki/Plasmid

http://en.wikipedia.org/wiki/Transduction_%28genetics%29

http://en.wikipedia.org/wiki/Bacterial_conjugation

TransformationTransfer and cloning of insulin gene

http://plantandsoil.unl.edu/croptechnology2005/gen/?what=animationList

TransformationTransfer and cloning of insulin gene

bacterial DNA