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Genetic diversity analysis of CWR in PortugalJoana Magos Brehm, Brian V. Ford-Lloyd, Nigel Maxted, Maria Amlia Martins-LouoJointPGRSecure/ECPGRworkshop: ConservationstrategiesforEuropeanCWRand LRdiversity79September2011,Palanga,Lithuania1Aims of this studyNational approachGenetic diversity studySelection of populations for conservationRelevant points and conclusionsOVERVIEW2

2262 CWR (mainland) (~77% of Portuguese Flora) populations are little genetically differentAMOVA (Analysis of Molecular Variance): Among populations: 8% Within populations: 92%OUTBREEDERPopulation genetic structure and differentiationDianthus cintranus subsp. barbatus

Dcb3Dcb1Dcb4Dcb2Dcb51863.99% of the total variance is explained by the first three coordinates.Dcb3 separates from the rest of the populations, while Dcb1 and Dcb2 cluster together and Dcb4 and Dcb5 make another cluster in all three principal coordinate combinations.

9786711000Dcb3Dcb1Dcb4Dcb2Dcb5NO GEOGRAPHIC PATTERNIsolation by distanceDianthus cintranus subsp. barbatus

Dcb3Dcb1Dcb4Dcb2Dcb5Mantel test => NO ISOLATION BY DISTANCE (no restriction to geneflow)19GENETICALLY VERY HOMOGENOUS!Dianthus cintranus subsp. barbatus

Dcb3Dcb1Dcb4Dcb2Dcb520Selection of populationsfor conservation1. Criteria used21DEMOGRAPHIC DATA: population sizeTHREAT DATA : number of threatsGENETIC DATA:Criteriagenetic diversity (measure of molecular diversity)# polymorphic alleles (measure of genepool richness)# common (population frequency >0.05) and localised alleles (in 0.05, and occurring in