Quantitative neuroanatomy meets quantitative genetics: Using ...
Genetic basis and analysis of quantitative...
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Genetic basis and analysis of quantitative traits
Mapping quantitative trait loci (QTL) – genetic architecture
Fine-mapping and cloning – molecular basis
1. What is the genetic architecture and molecular basis of phenotypic variation in natural populations?
2. Why is there phenotypic variation in natural populations?
Phenotype (P) = Genotype (G) + Environment (E)
Phenotypic variation – mutation & selection
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Correlate genotype with phenotype
1. Linkage mapping -QTL mapping-family data
2. Linkage disequilibrium-association study-population data
Mapping quantitative trait loci
A/aVar(E) = 1
A = 0; a = 10Var(G) = 25
A = 0; a = 0.1Var(G) = 0.1
A = 0; a = 1Var(G) = 1.0
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QTL mapping
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QTL mapping
i=abgiei
LOD=log10L a , b , 2/L A ,0, B1
2
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For single markers: LOD scores are confounded with the effect size
Interval mapping: Lander and Botstein 1989
Tight linkage or large effect Weak linkage or small effect
La ,b , 2=∏
i[Gi0Li0Gi 1 Li 1]
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LOD Thresholds for Significance
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Sample size for interval mapping
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Molecular basis of quantitative traits
QTL mapping
AssociationFine-mapping
Quantitative complementationReciprocal hemizygosity
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Model organism resources
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Fine-mapping
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Fine-mapping
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Quantitative complementation(addition or subtraction)
*
*
Addition (plasmid, transposon) Subtraction (deficiency, mutation)
*
( )( )
( )
( )
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Quantitative complementation(deficiency mapping)
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Deficiency mapping
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Deficiency mapping
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Reciprocal hemizygosity
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Bristle Number in Fruit flies: A model for quantitative traits
ST = sternopleural bristlesSC = scutellar bristlesAB = abdominal bristles
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(apparent) Stabilizing selection on bristle number
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Quantitative traits response to selection
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Selection on bristle number
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Linkage versus linkage disequilibrium
In a single generation (meiosis-family):Probability of no recombination = (1-θ)n
In two generations:Probability of no recombination = (1-θ)2n
In t generations:Probability of no recombination = (1-θ)nt
B1/B
2
A1/A
2
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Haplotype Frequency A1B1 x11
A1B2 x12
A2B1 x21
A2B2 x22
Allele Frequency A1 p1 = x11 + x12
A2 p2 = x21 + x22
B1 q1 = x11 + x21
B2 q2 = x12 + x22
Linkage Disequilibrium
D = x11 - p1 q1
correlation coefficient r = D
p1 p2q1q2B
1/B
2
A1/A
2
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Decay of linkage disequilibrium
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Linkage versus association
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Genetic variance (population) is a function of allele frequency
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Linkage disequilibrium
Lai et al (1994)
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Molecular Quantitative Genetics: Sporulation in Yeast
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Molecular Quantitative Genetics: Sporulation in Yeast
Deutschbauer and Davis (2005)RME1 – 1bp insertion in a mononucleotide repeat, noncodingTAO3 – missenseMKT1 – missense92% of variation explained
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Reciprocal Hemizygosity TestsAllele-Replacements
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Quantitative Trait Nucleotide Frequencies
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Genetic Interactions AmongQuantitative Trait Nucleotides
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Molecular Quantitative Genetics: Sporulation in Yeast
Deutschbauer and Davis (2005)RME1 – 1bp insertion in a repeat, noncodingTAO3 – missenseMKT1 – missense92% of variation explained
Ben-Ari et al (2006)RAS2 – 1bp insertion in repeat, noncodingPMS1SWS2FKH260 kb region on Chr14
Gerke et al (2009)RME1 – 1bp insertion in a repeat, noncodingIME1 – missense and noncodingRSF1 – missense80-90% variation explained
RME1 – Regulator of meiosis, TF represses IME1.
IME1 – Inducer of meiosis, TF.
RSF1 – Respiration factor, mediates transition to respiratory growth.
TAO3 – Transcriptional activator, involved in polarized morphogenesis.
MKT1 – Maintenance of K2 killer toxin.
RAS2 – GTP binding protein that regulates nitrogen starvation response, sporulation, filamentous growth.
PMS1 – ATP binding protein required for mismatch repair.
SWS2 – mitochondrial ribosomal protein of the small subunit.
FKH2 – Forkhead TF, regulates G2/M phase genes, silencing and transcriptional elongation.
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Linkage versus association
Method Frequency Effect size
Linkage rare large
Association common small