General InformationYear Plan, 2016, updated in 2018. ... 12 Mr. Rajendra Maharjan Electrician Part...
Transcript of General InformationYear Plan, 2016, updated in 2018. ... 12 Mr. Rajendra Maharjan Electrician Part...
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General Information
Name of the Institute:
Central Department of Biotechnology, Tribhuvan University
Address of the Institute:
Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu,
Nepal.
Type of University:
Government
Quality Assurance and Accreditation approval date:
22nd
Nov 2018
Year of Establishment of the Institute:
2008
Course commencement:
2009
Conducting courses:
M.Sc. Biotechnology (2009)/Ph.D. Biotechnology(2011)/Post Doc.(2018)
Name of the Head of the Institution:
Prof. Dr. Krishna Das Manandhar
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Introduction
Biotechnology is a cutting-edge technology as well as an applied science which can stabilize
the economy of the country. Furthermore, analysts have predicted that biotechnology will be
one of the most important applied sciences of the 21st century. Industrial countries and some
developing countries have already been benefited from the advancement in Biotechnology
improving plants, animals and microorganism and producing environment friendly products
in the form of food items, pharmaceuticals. It is also widely used in health, disease diagnosis
and treatment in addition to effective criminal investigation methods. Nepal being
agricultural country and rich in natural resources has all possibilities to break the ice. So
with the joint hands of efficient manpower in the field of biotechnology and proper national
strategies set by Tribhuvan University along with the knowledge acquired in this field and
provision of good research budget allocation can make it possible to harness the benefit of
the accumulated knowledge.
Central Department of Biotechnology was established in 2008 AD under Institute of Science
and Technology, Tribhuvan University(TU), Nepal, the premier university in Nepal. It
provides courses in diverse disciplines at various levels including modern biology subjects.
Considering the potential modern biotechnology, TU established Central Department of
Biotechnology hoping for a leading role in fulfilling the national demand of the manpower
and also to start innovative researches in various fields of biotechnology. The curriculum of
Master level in biotechnology has been formulated considering the need of the current
nation’s requirement as well as to meet the international standard in the subject. It is a
technological professional manpower preparation program as of other Institute of
engineering, Institute of agriculture & animal Science, Institute of Medicine and Institute of
Forestry programs of Tribhuvan University. Recently, this department has launched EMIS,
which keeps all the departmental information like data of students, teaching and non-
teaching staff data, store data, library and various activities going on in department.
Infrastructure (Land/building/lab equipment)
Central Department of Biotechnology, Institute of Science and Technology, Tribhuvan
University is located at Tribhuvan University Compound in Kirtipur Municipality of
Kathmandu valley of Bagamati Province. The Department is within the Science Block close
by the Central Library of the university
in the premises of Physics-Chemistry-
Computer-Biotechnology complex.
Site map of the Central Department of
Biotechnology, Institute of Science and
Technology,TribhuvanUniversit, Kirtipur
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It has approx 75m X 15 m (1125 sq mt) existing land space using as working building with a
big lawn in front side of around 500 sq mt [Fig. 2]
Current existing building area (The red line boundary area) of Central Department of Biotechnology,
Institute of Science and technology, Tribhuvan University. The building area is approx. 1125 Sq mt
and the lawn in front 500 sq mt.
Quality Assurance and Accreditation Certification
Department defined its vision mission, goal and objective as to meet the VMGO of
Tribhuvan University. This department is running in accordance to the road map set in Five
Year Plan, 2016, updated in 2018.
Department initiated the process for Quality Assurance and Accreditation from University
Grant Commission-Nepal from the year 2017 submitting the Letter of Intend (LOI). The Pre-
Review Team (Pre-PRT) from UGC inspected the Department extensively in January 04,
2018. The satisfaction to the Pre-PRT team led for the PRT visit in August 12, 2018
comprising a QAA Foreign Expert. The eight Bench Mark bases analysis of the Department
passed the criteria. Department is follows the policies and procedures of Tribhuvan
University to meet the Department policies. It is proud of its teaching learning, research, new
ongoing infrastructure, students support, information technology and public information
though there are lots of room to improve. Finally, Department received the QQA-Certificate
on November02, 2018. During the process of QAA and aftermath, Department has changed
lots of its activities improving the academic and research fundamentals. Among the 13
Central Departments under Institute of Science and Technology of the university, it has
emerged as one of the best Department and has moved for Center of Excellence with in
Tribhuvan University.
Central Department of Biotechnology
Tribhuvan University
Institute of Science and Technology
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A NOTABLE ACHIEVEMENT
IN THE YEAR 2018, November
22, 2018, Department got QAA
certificate issued by University
Grant Commission
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Motto, Vision, Mission and Objectives of the course
(Edited 2018)
Motto
Welfare of Nation and people through Biotechnology
Vision
World class Biotechnology institute for serving lives and nature through
1. An academic entity for biotechnology studies
2. An academic Centre of Excellence for Teaching-Learning and Research
3. Role model for transformation of research to nation development
Mission
Explore and conduct country specific research to contribute to science world
1.1. Well furnished attractive academic premises with enough academic and research
spaces
2.1 Establish CDBT as the first choice of bioscience study/teaching Explore the new
fields very
2.2 Equip institutional laboratory as nation’s model teaching laboratory
3.1 Emphasize on the application oriented researches
3.2 Publish original research articles in high impact factor cited journals
3.3 Produce salable manpower for national and international market
Objectives
An academic entity for biotechnology studies
1.1.1 Biotechnology Department, an academic and beautiful environmental premises.
1.1.2 Construction of building to accommodate required rooms and research spaces.
An academic Centre of Excellence
2.1.1 Develop/recruit qualified and skilled manpower (scientist/experts) in their respective
area of specialization in biotechnology.
2.1.2 Allow the students/faculties to participate international seminars and conferences as
well as bring faculties from abroad to lure the international students towards CDBT.
2.1.3 Frequent revisions of courses to make a competent syllabi and prompt examination
system.
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2.1.4 Launch new programs associated with biotechnology array alone or in affiliations
2.1.5 Attract SAARC/international students for academic degree on biotechnology
2.2.6 Equip the department with all types of essential, general ,advance and sophisticated
scientific instruments suitable for academic and innovative researches.
2.2.7 Develop as a Center of Biological Science Training for faculties/researchers/lab
technicians/students.
Research center of Excellence
3.1.1 Identify the researches according to the specialization of faculties and need for the
country.
3.1.2 Focus to medicinally important phytochemical compound, antibody production,
transgenic plant, vaccine production, animal production, medical diagnostic
tools, food technology etc.
3.2.1 Conduct research projects either alone or in collaboration with national and
international institutions and give message to scientific community of the world..
3.3.1 Encourage the M.Sc. students, Ph.D. scholars, post doctorate degree and faculties to
give high degree performances utilizing the facilities available in the Department.
3.3.2 Allow the students and research scholars learn the knowledge and skills of advance
facilities of the Dept.
Major Activities to achieve the objectives
1.1 New Biotechnology building under completion .
2.1.1 Three faculties are facilitated for Ph.D. and 1 recently completed.
2.1.2 Students and faculties are having opportunities to participate in ICGEB/NIH like
programs.
2.1.3 Syllabus revision for 3 times and now working for next latest revision
2.1.4 New course M.Sc. Virology has been approved and yet to develop syllabus. Now
thinking of M.Sc. Bioinformatics.
2.1.5 Enquiries from SARC for Ph.D. and M.Sc. Looking forward for admission.
2.2.1 Equipping the lab is ongoing process Plan to have highly sophisticated instruments
like NGS, HPLC, Ultra centrifuge under resource Mgmt Program of HERP.
2.27 Teachers’ training in Experimental Biotechnology as winter school.
3.1.1 Course based on specialization of faculties Eg. Dengue virus, bacteriophage, biofuel.
3.1.2 Carrying some applied researches on biofertilizer, beverages, disease diagnosis etc.
3.2.1 International Collaboration with LJI, ICGEB etc.
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3.2.2 High degree performances. Eg. Cutaneous leishmaniasis, Biofertilizer
Comparative progresses
Physical Infrastructure
Central Department of Biotechnology started foundation of its building but was stopped for
a long time. After the QAA the building is at the verge of completion of its first phase of
work, the ground floor of middle portion of the design. All the works except marble flooring
and painting have been completed. The total land area is about 15000 sq mt with on going
building constructed area 2000 sq. mt.
The ongoing new building construction of Central Department of Biotechnology. A. Land area
behind the construction B. Current status of the constructed building at the verge of completion.
List of faculties/Expertise/support staff Available
The Central Department provisioned for 18 faculties (Academicians) and 11 staffs for
administrative, technical and supportive section. Presently it has only ten faculties. Visiting
faculties are being outsourced as experts from outside according to need of specific subjects
and course cycle. Distance classes from different countries are carried out using skype/hangs
out facilities. Similarly, the staffs required for different specific purposes are also hired as
daily attendance based staffs like electrician, night gate keeper and support staff and now are
14. Ratio of Full time Teaching and Non-Teaching is 0.83:1.
List of Existing Faculties
SN
Name of Faculty Designation Status Highest Academic Qualification
1 Prof. Krishna Das Manadhar Head Permanent Ph. D./Post Doc.(Immunology/
Animal biotechnology)
2 Prof. Ganga Kharel Professor Permanent (on
sabbatical leave) Ph. D. (Food biotechnology)
A B
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3 Prof. Rajani Mallla Former Head Permanent Ph. D. (Microbiology)
4 Jarina Joshi Lecturer Permanent Ph. D. (Bioprocess)
5 Bal Hari Poudel Lecturer (on Ph.D. Leave) M. Sc. (Biotechnology)
6 Smita Shrestha Lecturer (on Ph. D. leave) B.V.Sc/M.Sc. (Medical
Biotechnology)
7 Pragati Pradhan Lecturer Permanent M. Sc. (Biochemistry)
8 Dr. Suresh Subedi Lecturer Permanent Ph. D. (Bioprocess)
9 Preeti Regmi Lecturer (on Ph. D. leave) M. Sc. (Biotechnology)
10 Alina Shree Sapkota Lecturer Permanent M. Sc. (Biochemistry)
List of regular visiting faculties as experts
SN
Name of Faculty Designation Status Highest Academic Qualification
1 Prof. Gauri Shankar
Manandhar Visiting faculty Part-time Ph. D. (Cell biology)
2 Dr. Pramod Aryal Visiting faculty Part-time Ph. D. (Genetic engineering)
3 Dr. Bhusan Shrestha Visiting faculty Part-time Ph. D. (Agriculture biotechnology)
4 Prof. Mukunda Ranjit Visiting faculty Part-time Ph. D. (Plant biotechnology)
5 Dr. Rohit Pokhrel Visiting faculty Part-time Ph. D. (Medical biotechnology)
6 Dr. Umesh Mandal Visiting faculty Part-time Ph. D. (Animal Biotechnology)
7 Dr. Mahesh Adhikari Visiting faculty Part-time Ph. D. (Agriculture biotechnology)
8 Other Faculties Temporary Part-time Different Subjects
List of staffs
SN
Name of Staff Post Status
1 Mr. Bhupendra Lal Shahi Section Officer Permanent
2 Ms. Sumitra Manandhar Account Officer Permanent
3 Mr. Ujjwal Man Pradhan Head account assistant Permanent
4 Mr. Madanman Singh Rokaya Head assistant Permanent
5 Ms. Elen Pradhan Head Lab assistant Permanent
6 Mr. Sailendra Koirala Head Lab assistant Permanent
7 Ms. Til Kumari Gautam Technical lab assistant Permanent
8 Ms. Binu Tamang Technical lab assistant Permanent
9 Ms. Subha Maya Magar Support staff Permanent
10 Ms. Maiya Khanal Support staff Permanent
11 Mr. Guna Nidhi Dhakal Support staff Permanent
12 Mr. Rajendra Maharjan Electrician Part time
13 Mr. Pan Bhai Support staff (Rest room
maintenance) Part time
14 Mr. Lalit Gurung Gate keeper Part time
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Comparative summary table of Full time teaching staff
Full Time
Teachers
Disadvantaged /
Janajatis Others Grand
Total
From
outside
Ktm
Valley Female Total Female Total
Before
QAA
Total 4 6 2 6 12 4
With PhD 1 3 - 3 6 2
Enrolled PhD 1 1 - - 1 -
With Masters 2 2 2 3 5 2
After
QAA
Total* 4 5 2 5 10 4
With PhD 2 3 - 2 5 2
Enrolled PhD 1 1 1 2 3 1
With Masters 1 1 1 1 2 1
Comparative summary table of Full time non-teaching staff
Staff Category
Disadvantaged /
Adivasi Janajatis Others Grand
Total
From
outside
Kathmandu
Valley Female Total Female Total
Administrative Staff 1 4 1 4 8 4
Technical Staff 1 1 2 3 4 3
Total 2 5 3 7 12 7
Teaching Learning Schedule
Department Academic has developed academic Calendar based on the calendar published by
Institute of Science and Technology. The total working days per semester will not be less
than 100 days. Department created environment for the regular and research students to
work from early morning to late evening
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Day class work Evening time of lab work
Infrastructure, facilities and IT support
Department has existing building in the Physics-Chemistry-Computer-Biotechnology
Complex and a new separate building at the verge of completion. The existing building is
cozy but well managed and utilized spaces for the courses running in the Department. There
is only one class room which is being used both for two semesters by managing the practical
classes in two shifts. Even the seminar room is used appropriately for classes whenever
remain free. Two M.Sc. general laboratories are equipped for running two groups of students
every day in the morning and afternoon sessions. Severn rooms/compartments are
designated for specific research purposes. One Department Library is equipped with about
700 reference books. Store rooms and administrative rooms are for serving the daily
requirements for the students, faculties and staffs. There is only one rest room within the
Department premises, however, the rest rooms in the Physics and Computer Department are
also available for use. There are altogether 17 specific defined rooms/compartments in the
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existing building facilitated with equipments [Table 8]. It is obvious that the spaces are not
enough for advance scientific equipment in the Department so it is managing the lack of
spaces by sharing the available rooms for necessary purposes. The hope “new building for
the Department” is under construction.
Physical facilities of the Central Department of Biotechnology.
Sections of Buildings Numbers
Class room/ seminar room 2
Administrative rooms / Account section 2
Store room 2
Library 1
Fresh/Wash room 1
M.Sc. laboratories
Immunology and Microbiology/Medical Biotechnology/ Bioprocess
Cell Biology and Genetics/Animal Biotechnology/ Food
Biotechnology
Bioinformatics/Seminar room
2
Research laboratories
Molecular Biology/ Genetic Engineering
Tropical Infectious Disease and Virology
In vitro fertilization/ Phyto-Bioprospecting
Bacteriophage/ Streptomyces
Bio-fuel engineering
Plant/Fungal Tissue Culture
Biofertilizer/Biopesticide
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Total 17
Laboratory Facilities
The CDBT though a new department of Tribhuvan University at its initial stage, is equipped
with the modest and sophisticated instrumental facilities necessary to run the basic and
advanced research on the different disciplines of biotechnology. There were two general
laboratories of
1. Lab 1: Microbiology/Immunology/Bioprocess/Food/Medical Biotechnology
2. Lab 2: Cell biology and cytogenetics/Biochemistry/Animal Biotechnology
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Masters’ student’s Laboratory in Central Department of Biotechnology. A. Laboratory 1. B. Laboratory 2.
Department provisioned s research laboratories after QAA certification. Department has 7
small rooms/chambers. Based on the researches carrying by the faculties, the laboratories are
1. Molecular Biology/Genetic Engineering,
2. Tropical Infectious Disease and Virology,
3. In vitro fertilization/ Phyto-Bioprospecting,
4. Bacteriophage/ Streptomyce,
5. Bio-fuel engineering,
6. Plant/Fungal Tissue Culture and
7. Biofertilizer/Biopesticide.
Research laboratory of Central Department of Biotechnology.
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Laboratory with equipments in Tropical Infectious Disease and Virology laboratory
Laboratory with equipments in Natural product and drug design laboratory
Laboratory with equipments in Molecular Biology/Genetic Engineering, Bacteriophage and
Biofuel laboratory
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Instrument facilities:
The CDBT is equipped with all basic equipment necessary for M.Sc. biotechnology practical
works and for some of the advance equipments for PhD students. Some major equipment
installed in the department are: Flow Cytometry, Real Time PCR, Lyophilizer, Fluorescent
microscopes, Nanodrop, Biosafety cabinet, Cell coulter, Fermenter, ELISA plate reader and
washer, Thermal cyclers, Spectrophotometer, -80oC Freezer, CO2 incubator, Growth
chambers, Shaking incubators, Phase Contrast Microscope with photograph facility etc. The
equipment installed in the Department explain its high efficiency in the scientific research
works of present day world and gives the glimpse of laboratory in developed countries. The
department is in the process of purchasing Next Gen Sequencing (NGS), Ultracentrifuge and
High Performance Liquid Chromatography (HPLC) in this fiscal year. Still the most advance
equipment are sought which in our opinion should be facilitated as central facility by
Tribhuvan University for all scientific researchers. This department got continuous support
from different sectors for instrument collection.
List of major instruments installed in the Department
SN Instruments Brand Funding Source
1 Flow cytometry BD NIH, USA
2 Real Time PCR BioRad HERP, UGC
3 Lyophilizer ZIRBUS Department
4 Fluorescent microscopes,
Compound
ZEISS HERP, UGC
5 Fluorescent microscopes, Inverted BEL Hombolt
6 Nanodrop Shimadzu HERP, UGC
7 Biosafety cabinet BioBASE HERP, UGC
8 Fermenter Electrolab Department.
9 ELISA plate reader and washer Thermo Department., HERP-
UGC
10 Thermal cyclers BioRad Department / HERP-
UGC/ LJI-Dengue
Project
11 Spectrophotometer Chemito Department, TWAS
12 -800C Freezer GFL TWAS
13 CO2 incubator Memmert Department
14 Growth chambers Eliete/Sonar Department
15 Phase Contrast Microscopes with
photograph facility
BEL Department
16 Oxygen, Nitrogen gas supply
system
Shanker Gas Department
17 Cooling outswing centrifuge (Not
fuctional)
Hitachi Hombolt
18 BOD incubator OpticsTechnology UGC/ Department
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19 Shaking incubator Nauyog, India TU planning
20 Gel Doc MS major IRLASP+Department
21 Cooling Centrifuge YINGEAI
Instrument
LJI-Dengue Project
22 Cell coulter Elumina HERP
23 Chiller for rotavapor JKA UGC Project
24 Freezers (#2) Associated
Scientific
Technology/
DELTH
CDBT, TU
New arrivals after QAA process
Flow Cytometry BOD incubator
Biosafety cabinet Shaking incubator
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Cooling centrifuge PCR machines
Previously installed instruments
Florescent microscope Phase contrast microscope
Lyophilizer and Nano Drop
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More other instruments available in the department
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Classroom-Teaching /Learning Facilities
Class room is well equipped with audio-visual (power-point projection) facilities supported
by wi-fi for internet access. Along with the advanced tool, the following conventional
teaching tools are also available in the lecture room.
Smart Board
LCD-Projector
Overhead Projectors
White board–Marker
Classroom with IT facilities available in the department
Class room lecture and Seminar cum class room
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Research Activities
Before QAA [2009 – 2017]
International Projects (Faculties)
Renewable Nepal program [NORAD ] – Prof. Tribikram and Dr. Jarina Joshi
1. Development of Recombinant Sacharomyces cervicae by expressing xylose
transporter and xylose isomerase for efficient multiple sugar metabolism. 2012
2. Design of microbial fuel cell to produce ethanol from lignocellulosic biomass and
greenery waste and hence to produce electricity from fuel cell. 2012
Natural Product and Drug Development. Prof Krishna Manandhar
3. Bio-prospecting on Biological Materials of Nepal. Collaborators: Korea Research
Institute of Bioscience & Biotechnology (KRIBB), Korea; and Ethnobotanical
Society of Nepal (ESON)-2013
National Projects
1. Retrospective analysis of EGFR mutation in lung cancer patient. 2011. Prof. Mohan
Kharel. Supported by UGC.
2. Identification of antigenic protein from Leishmania donovani as marker for diagnosis
of visceral leishmaniasis.2011. PI: Dr Krishna Das Manandhar Supported by UGC.
3. Morphogenic and Genotoxic Effects of Insecticides on Vicia faba and Allium cepa
used by Farmers in Kathmandu Valley. Supported by UGC. PI: Dr Sampoornananda
Jha. 2011. UGC.
4. Screening of medicinal plants having antileishmanial property. Supported by
National Academy of Science and Technology (NAST). PI: Dr Krishna Das
Manandhar. 2012. NAST
5. Detection of BRCA1 mutation in breast cancer cases in Nepal: a pilot study. 2012.
Supported by UGC.
6. Analysis of anticancer effect of different indigenous medicinal plants of Nepal.
Supported by Ministry of Education PI: Bal Hari poudel
7. Muscular Distrophy study on Nepalese patients by continuous PCR. Supported by
Institute of Medicine, TUTH, IOM, T.U. PI: Dr. Smita Shrestha Chhetri. 2012.
8. Production of Ethanol in Electrochemical cell. Supported by UGC, Nepal. PI: Jarina
Joshi 2013.
9. Screening of medicinal plants having flavonoid, antioxidant and anticancerous
properties . Supported by UGC, Nepal.
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Status of Ongoing Projects of the Institute:
International Projects
Department started to receive the international projects from the year 2012 and it was
supported by the Norwegian Agency for Development Cooperation- Nepal Chapter in
renewable energy for Prof. Tribikram Bhattarai and Dr. Jarina Joshi, In 2013 a project of
Prof. Krishna Das Manandhar funded by Korea Research Institute of Bioscience &
Biotechnology (KRIBB), Korea had been conducted. Similarly, Prof. Manandhar received a
grant from ICIMOD to develop proposal on study overall quality of water from Nepalese,
Bhutanese and Indian Himalayas and its health impacts on dependent population in 2018.
The Dengue project is an examplenary for international network development in research.
Prof. Manandhar who worked as Fulbright Senior Research Scholar in La Jolla Institute for
Allergy and Immunology, Calaifornia, USA in 2015/2016 could develop and continue the
work as a five year project from 2016-2020. Based on the grant support, The Tropical
Infectious Disease and Virology Lab (TIDAV Lab) of the Department is able to support
many M.Sc. theses and a PhD. on Dengue disease.
TIDAV Lab is continuously applying the NIH Grants forming a international groups,
however, success is still awaited. Interestingly, in 2019, Department could join hands with
Dr. Manju Jain from Central University of Punjab using the research data generated by Prof.
Manandhar’s Lab for Nepal chapter to apply for a international collaborative research grant
application called by International Centre for Genetic Engineering and Biotechnology
(ICGEB)-Italy. The project is accepted and is the ever first grant of its kind. Hence,
Department has entered to the international competitive grant application showing its
competent international strength. Currently there are 3 International Projects running in the
Department and all are of Prof. Manandhar.
Ongoing International Projects in Central Department of Biotechnology
Year Title Funding body Grants Status
Dec 2016-
Dec 2020.
Dengue – CDBT-LJI-KARIUS
Project. PI – Prof. Krishna Das
Manandhar, International
Collaborator: Dr. Sujan, USA
Dengue fever and Dengue like
illness in Nepal.
La Jolla Institute
for Allergy &
Immunology and
KARIUS Inc. USA
25000 USD Ongoing
2020 – 2022
Leishmaniasis – ICGEB Project,
PI – Dr. M Jain, Central
University of Punjab, CoPIs:
Prof. Krishna D Manandhar, and
Prof. Greg Matlewenski, Cananda
A Molecular. & Immunological
Investigation of leishmaniasis
from an unusual foci of cutaneous
and visceral disease in India and
Nepal .
International Center
for Genetic
Engineering and
Biotechnology
(ICGEB), Trieste,
Italy
39000 Euro Accepted and
yet to start
2019-2020 Epstein Barr virus- Switzerland Prof. Cristian Lab, Reagents At the end of
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Pilot Project, PI- Prof. Krishna
Das Manandhar and Prof. Cristian
Munz.
Screening of Epstein Barr virus
and Kaposi Sarcoma antiserum
in Nepalese healthy population
and molecular diagnosis
Immunology
Department,
University of
Zurich and Tropical
Infectious Disease
and Virology lab,
CDBT-TU
only project
National Projects
Department has envisaged at least one project for each faculty in its five year plan developed
in 2012 which continued in its next plan 2016. As per the vision, all the faculties are having
their own projects either of international or national. Department boast University Grant
Commission Nepal for making available of the collaborative research project (CRP) grants
with good sum of funds to start a good research. This Department is proud enough to get two
such grants and are running very smoothly in the departmental laboratory. Further
Department highly appreciate the effort carried by each faculties to bring research projects.
Currently there are 7 national grants running funded all most all by UGC-Nepal.
Ongoing national Projects in Central Department of Biotechnology
Year Title Funding body Grants Status
2018 - 2020
(2074 - 2076
B.S.)
Streptomyces – UGC collaborative
Project. PI – Prof. Rajani Malla.
Biodiversity of Streptomyces from
soil collected from various parts of
Nepal and screening for potent
bioactive compounds.
UGC Institutional
Grant
NRs.
2,000,000.00
At the end of
project
2019-2022
(2075 –
2078 B.S.)
Dengue – UGC collaborative
Project. PI – Prof. Krishna Das
Manandhar. Profiling Cellular
Immune Responses in Dengue Virus
Infected Nepalese Population.
UGC Institutional
Grant.
NRs.
2,000,000.00 Ongoing
2019-2020
Ayurved Drug – National
collaboration. PI- Prof. Krishna Das
Manandhar Cytotoxicity and Anti-
cancer activities of “Heerak
Bhasma” (Diamond Particles) on
different cell lines.
National Ayurveda
Training and
Research Center,
Gov. of Nepal.
NRs.
200,000.00 Ongoing
2020-2022
PI- Ms. Jarina Joshi:Design of
Microbial fuel Cell to Manage
Household Organic Wastes Using
Mixed Culture of Microbes.
Faculty Research
Grant by UGC
NRs.
400,000.00 Ongoing
2020-2021 PI – Dr. Suresh Subedi Screening of
Novel Genes Involved in Biofilm-
Small Research
Development and
Innovation Grant by
NRs.
200,000.00 Ongoing
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mediated Carbapenem Resistance
in Pseudomonas aeruginosa.
UGC
2020-2021
PI – Ms. Pragati Pradhan.
Application of Bacteriophage to
treat the multidrug resistant
bacteria infection in mouse model
Small Research
Development and
Innovation Grant by
UGC
200,000.00 Pilot work
Ongoing
2020
PI – Dr. Pramod Aryal. Molecular
charaterization of plant pathogen
and control (plant tissue culture
model and field trial). Focus on
Coffee Leaf Rust, Fall Army Worm
and Dadhuwa disease of L.
cardamom.
Department and
targeted to propose
in UGC grants
Reagents Pilot work
Ongoing
Besides the faculties, the Ph.D. and M.Sc. students are also carrying their separate projects
for their respective thesis works supervised by their supervisors (Faculties). The trends of
receiving thesis grants to the students of the Department are very encouraging. All the six
Ph.D. students’ projects are being funded by different institutions except the recent enrolled
ones.
Ongoing research projects with funds carrying by Ph.D. students in the Central Department of
Biotechnology.
Year Title Funding body Grants Status
2016 - 2020
Characterization of TNF-alpha gene
polymorphism and its impact in
Influenza A/Pandemic( H1N1)
patients in Nepal
National Public
Health
Laboratory,
Kathmandu
All facilities
in kinds On Going
2017-2020
Molecular and immunological
characterisation of hepatitis- e virus in
Nepal
UGC- Nepal NRs.
600,000.00 On Going
2018-2020 Characterization of Bacteriophage
against Multidrug Resistant Bacteria.
UGC-
Nepal/NAST
NRs.
600,000.00 On Going
2017-2021 Effect of medicinal plants on the
immune response of stem cells. NAST
All facilities
in kinds On Going
2018-2022
Role of Selective immunological
Marker for Dengue Severity in
NepalRole of Selective immunological
Marker for Dengue Severity in Nepal
UGC- Nepal NRs.
900,000.00 On Going
2019-2024
Designing of Transdermal Patch from
Natural Polymer and Oils for
Rheumatoid Arthritis Therapy
UGC- Nepal NRs.
900,000.00 On Going
Similarly, the students carrying M.Sc. thesis works are also being funded by the different
institutions like UGC-Nepal, Nepal Youth Council, Nepal Academy of Science and
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Technology (NAST) and Central Department of Biotechnology itself. There are 10 projects
supported by the other national institution besides CDBT [Table 7].
Ongoing research projects of M.Sc. students with funds from national funding organizations
carrying at the Central Department of Biotechnology.
Year Title Funding body Grants Status
2019 Cutaneous Leishmaniasis UGC 50,000.00 Ongoing
2019 Chronic Myeloid Leukemia UGC 30,000.00 Ongoing
2019 Dengue- Cytokine Profiling UGC 30,000.00 Ongoing
2019 Bio-film mediated antibiotic
resistance
National Youth
Council Nepal 50,000.00 Ongoing
2019 Dengue – Host Genetics UGC 50,000.00 Ongoing
2019 Dengue – Vaccine candidate
development
Chinese Academy
of Science 80,000.00 Ongoing
2019 Bioethanol Production National Youth
Council Nepal 50,000.00 Ongoing
2019 Isolation of BT strain and
identification of VIP gene NAST In Kinds Ongoing
2019 Biodegradation of Lignocllulose
Biomass using microogranism NAST In Kinds Ongoing
2019 Anti diabetic effect of Plant
extracts NAST In Kinds Ongoing
Ph.D. Graduates
2016 Graduation
1. Molecular characrerization of Lactobacillus spp. from Diiferent parts of Nepal
Ranjan Koirala 2012 Prof. Rajani Malla and Dr. Sangeeta Shrestha
2018 Graduation
2. Immunological and Molecular Epidemiology of Emerging and Circulating Viral
Diseases of Nepal
Birendra Prasad Gupta 2013 Prof. Krishna D Manandhar
2019 Graduation
3. Molecular cloning of thermophilic Cellulase
Poonam Yadav 2013 Prof. Tribikram Bhattarai and Dr. Jyoti Maharjan
4. Study On Immune Reconstitution Inflammatory Syndrome (Iris) In Hiv/Aids
Infection
Shrawan Kumar Mishra 2013 Prof. Krishna D Manandhar
2020 Graduation
5. Enhancement in ethanol production by electrochemical redox combination of
microbial cells from lignocellulosic biomass.
24
Jarina Joshi 2013 Prof. Tribikram Bhattarai and Prof. Amar Yadav
Ongoing Ph.D.
1. Characterization of TNF-alpha gene polymorphism and its impact in Influenza
A/Pandemic( H1N1) patients in Nepal
Bimlesh Jha 2015 Prof. Krishna D Manandhar
2. Molecular and immunological characterisation of hepatitis- e virus in nepal
Smita Shrestha 2015 Prof. Krishna D Manandhar
3. Characterization of Bacteriophage against Multidrug Resistant Bacteria.
Guna Raj Dhungana 2016 Prof. Rajani Malla
4. Effect of medicinal plants on the immune response of stem cells.
Bhuvan Saud 2017 Prof. Rajani Malla and Dr. Kanti Shrestha
5. Role of Selective immunological Marker for Dengue Severity in NepalRole of Selective
immunological Marker for Dengue Severity in Nepal
Ramanuj Rauniyar 2018 Prof. Krishna D Manandhar
6. Designing of Transdermal Patch from Natural Polymer and Oils for Rheumatoid
Arthritis Therapy
Prasansha Pant 2018 Prof. Rajani Malla and Prof. Dr. Rameshwar Adhikari
7. Nutritious values of fermented vegetables
Rajesh Shrestha 2019 Prof. Ganga Kharel
8. Febrile of Unknow origin
Dr. Eans Tara Tuladhar 2020 Prof. Krishna D Manandhar
9. Epstein Barr Virus & Nasopharyngeal Carcinoma
Soma K Baral 2020 Prof. Krishna D Manandhar
10. Anti MDR Bacteriophage isolation and characterization
Deependra Mandal 2020 Prof. Rajani Malla
Researches on national issues
Local Farmer Mr. Rajendra Kunwar
and friends From Jumla District of
Nepal interacting with faculties of
CDBT for protection of indigenous
threatened walnut plant found in
Jumla District
25
Research team and the Boipesticide and Biofertilizer developed in the Department using the
cocktails of bacteria
26
Research team of Tropical Infectious and Viral Disease Laboratory in the Department and
coming in front line in the national issue of infectious and viral diseases
27
Publications: Comparison before and after QAA
Regular projects and thesis are running in the Department. All the faculties are equally active
to write manuscript and publish paper. Most of the papers are published in reputed
international journal though TU policy has not given any credit hour for doing project,
guiding thesis and making manuscript.
Number of publications: In the first five years of the Department establishment i.e. 2009-
2013, it could publish only 7 international and 16 national research articles..In the next three
years (2014-2016) the ratio reversed and there were 23 international and 3 national. The 3
senior faculties retired after then and replaced by fresh faculties. Despite the situation,
Department could increase its publication number during and after the initiation of QAA
hiking them to 29 (25 international and 4 national). The data showed that the researchers
both students and faculties wished to published in international journals in comparison to
national ones. In the Departmental history, it has published 79 papers in total comprising 55
international and 23 national papers.
Publication Status of department (Phase wise and year wise)
2009-2013 2014-2016 2017-2019
7
23 25
16
3 4
Publication in three phases of Departmental history
International National
Year Int Nat Total
2019 8 1 9
2018 9 1 10
2017 8 2 10
2016 7 1 8
2015 8 0 9
2014 8 2 10
2013 1 7 8
2012 2 5 7
2011 2 1 3
2010 1 2 3
2009 1 1 2
Total 55 23 79
1st 5 yr before QAA after QAA
28
Research Papers published from establishment year (2009) to 2019
2019
1. Shrestha, Mitesh & Khatri-Chhetri, Medha & Poudel, Ram & Maharjan, Jyoti & Dumre, SP & Manandhar, Krishna & Pandey, Basu & Pun, Sher & Pandey, Kishor. (2019). Molecular evidence supports the expansion of visceral leishmaniasis towards non- program districts of Nepal. BMC Infectious Diseases. 19. 10.1186/s12879-019-4083-3. (Peer-reviewed journal)
2. Sunil Timilsena, Sakkarin Ardsiri, Surada Lerdwana, Krishna Das Manandhar, Kovit Pattanapanyasat, Egarit Noulsri (2019). Accuracy of lymphocyte counts from UniCel DxH 800 in β-thalassemia/HbE patients having various numbers of nucleated red blood cells. Asian Pac J Allergy Immunol. doi: 10.12932/AP-170119-0472 (H index, Impact factor 1.747)
3. Bastola, Anup & Shrestha, Mitesh & Lamsal, Mahesh & Shrestha, Srijan & Prajapati, Sabita & Adhikari, Anurag & Gupta, Birendra & Hide, Mallorie & Devkota, Lina & Chalise, Bimal & Pandey, Kishor & Manandhar, Krishna. (2019). A case of high altitude cutaneous leishmaniasis in a non-endemic region in Nepal. Parasitology International. 74. 101991. 10.1016/j.parint.2019.101991. (Impact factor 2.017, SCI Rank 0.849)
4. Bhuvan Saud, Rajani Malla and Kanti Shrestha. Stem Cell Therapy in Nepal: Challenges and Opportunities. Hosa Journal of Stem Cells Research, Development and Therapy. 2019. DOI: 10.24966/SRDT-2060/100022. (Indexed journal)
5. Bhuvan Saud, Rajani Malla and Kanti Shrestha. A Review on the Effect of Plant Extract on Mesenchymal Stem Cell Proliferation and Differentiation. doi: 10.1155/2019/7513404, 2019 (Impact factor- 4)
6. Jarina Joshi, Pradip Dhungana,Bikram Prajapati, Rocky Maharjan, Pranita Poudyal, Mukesh Yadav, Milan Mainali, Amar Prasad Yadav, Tribikram Bhattarai and Lakshmaiah Sreerama (2019). Enhancement of ethanol production in electrochemical cell by Saccharomyces cerevisiae (CDBT2) and Wickerhamomyces anomalus (CDBT7). Front. Energy Res. - Bioenergy and Biofuels. 7:70. (Q2 journal)
7. Mukesh Yadav, Garima Bista, Rocky Maharjan, Pranita Poudyal, Milan Mainali, Lakshmaiah Sreerama, Jarina Joshi (2019). Secretory laccase from Pestalotiopsis species CDBT-F-G1 fungal strain isolated from high altitude: Optimization of its production and characterization. Appl. Sci. 9: 340. (Q2 journal)
8. Kathleen m. Kelsey, Michal Zigo, Winston E. Thompson, Karl Kerns, Gaurishankar Manandhar, Miriam Sutovsky, Peter Sutovsky (2019). Reciprocal surface expression of arylsulfatase A and ubiquitin in normal and defective mammalian spermatozoa. Cell and Tissue Research. DOI: 10,1007/s00441-019-03144-1. (Impact factor- 3.043)
2018
29
9. Shrestha M., Pandey BD., Maharjan J., Tiwari PN., Manandhar KD, Pun SB, Dumre S., Pandey
K. (2018). A Case of Visceral Leishmaniasis from the Non-endemic Himalayan region of
Nepal. Parasitology Research . 2018 Jul;117(7):2323-2326. doi: 10.1007/s00436-018-5887-6.
Epub 2018 May 2.
10. Sunil Kumar Sah, Joaquin V. González, Sadina Shrestha, Anurag Adhikari, Krishna Das
Manandhar, Shyam Babu Yadav, David A. Stein, Birendra Prasad Gupta and María Alejandra
Picconi. Human papillomavirus genotype distribution in cervical cancer biopsies from
Nepalese women. Infectious Agents and Cancer (2018) 13:4 DOI 10.1186/s13027-018-0176-7.
11. Seroepidemiology study of Hepatitis B virus infection in Nepal Smita Shrestha1,Sudhamshu
KC2,Balram Gautam2,Sher Bahadur Pun3,sila Mahatara1,Krishna Das Manandhar.SciUn
research journal,2018
12. Gupta BP, Tuladhar R, Kurmi R, Manandhar KD. Dengue periodic outbreaks and
epidemiological trends in Nepal. Annals of Clinical Microbiology and Antimicrobials.
2018;17:6. doi:10.1186/s12941-018- 0258-9
13. P Yadav, S Korpole, GS Prasad, G Sahni, J Maharjan, L Sreerama,. Morphological, enzymatic
screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of
Myagdi, Nepal 2018. Journal of Applied Biology & Biotechnology Vol 6 (3), 1-8
14. Birendra Prasad Gupta, Mahesh Lamsal, Sudhikshya Chaulagain, Ramanuj Rauniyar, Rajani
Malla, Smita Shrestha, Roshan Kurmi,Krishna Das Manandhar. Emergence of dengue in Nepal.
VirusDis. 2018.https://doi.org/10.1007/s13337-018-0439-3
15. Punam Yadav, Suresh Korpole, Gandham S Prasad, Girish Sahni, Jyoti Maharjan, Lakshmaiah
Sreerama, Tribikram Bhattarai (2018) Morphological, enzymatic screening, and phylogenetic
analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal. Journal of
Applied Biology & Biotechnology Vol. 6(3), pp. 1-8, May-Jun, 2018
16. Birendra Prasad Gupta, Mahesh Lamsal, Sudhikshya Chaulagain, Ramanuj Rauniyar, Rajani
Malla, Smita Shrestha, Roshan Kurmi,Krishna Das Manandhar. Emergence of dengue in Nepal.
VirusDis. 2018.https://doi.org/10.1007/s13337-018-0439-3
17. MiteshShrestha ,HemantaKumari Chaudhary, Pradip Kumar Chaudhary,Bal Hari Poudel*
Sequence Analysis of Rifampicin Resistance Determining Region (RRDR) of Mycobacterium
tuberculosis journal of tropical lifesciencesVOL. 8, NO. 1, pp. 87 – 90, 2018
2017
18. Shravan Kumar Mishra, Sundar Khadka, Subhash Dhital, Raj Kumar Mahto and Krishna Das
Manandhar*.Biomarkers in Immune Reconstitution Inflammatory Syndrome (IRIS) among
People Living with Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome
(HIV/AIDS). J AIDS Clin Res 2017, 8:9 DOI: 10.4172/2155-6113.1000728
19. Ananta Shrestha, Anurag Adhikari, Manjula Bhattarai, Ramanuj Rauniyar, Jose D. Debes,
André Boonstra, Thupten K. Lama, Mamun Al Mahtab, Amna Subhan Butt, Sheikh
Mohammad Fazle Akbar, Nirmal Aryal, Sapana Karn, Krishna Das Manandhar and Birendra
Prasad Gupta. Prevalence and risk of hepatitis E virus infection in the HIV population of Nepal.
Virology Journal.2017. 14:228 DOI 10.1186/s12985-017-0899-x
20. Krishna K. Shrestha, Yadu N. Paudel, Krishna D. Manandhar, Gyandra P. Ghimire2 Sangho
Choi and Sabina Shrestha. Bio-prospecting of plant resources for validation of indigenous
knowledge and the search for novel herbal drugs in Nepal. Madhav Karki, Rosemary Hill,
Dayuan Xue, William Alangui, Kaoru Ichikawa and Peter Bridgewater (eds.). 2017. Knowing
30
our Lands and Resources: Indigenous and Local Knowledge and Practices related to
Biodiversity and Ecosystem Services in Asia.Knowledges of Nature 10. UNESCO: Paris. pp.
200. France. 157-168
21. Neupane, Surendra , Sijapati, Jaishree , Bhattarai, Tribikram Shrestha, Sangita (2017)
Genetic diversity in Nepalese population of Swertia chirayita (Roxb. Ex Fleming) H. Karst
based on intersimple sequence repeats (ISSR) markers. Afr. J. Biotechnol. 16(16), 895-907
22. Baidya Nath Jha, Mitesh Shrestha, Durga Prasad Pandey, Tribikram Bhattarai, Hari Datta
Bhattarai and Babita Paudel (2017) Investigation of antioxidant, antimicrobial and toxicity
activities of lichens from high altitude regions of Nepal. BMC Complementary and Alternative
Medicine 17:282-289
23. Punam Yadav, Bhagwat Rimal, Jyoti Maharjan, Sujan Shrestha, Lakshmaiah Sreerama,
Tribikram Bhattarai (2017) “Physico-Chemical Analysis of Hot Springs of Myagdi District,
Nepal for the Balneotherapy and Drinking Purposes”. INTERNATIONAL JOURNAL OF
ADVANCED BIOTECHNOLOGY AND RESEARCH 8: 699-709’
24. HemantaKumari Chaudhary1 , Mitesh Shrestha , Prakash Chaudhary , Bal Hari Poudel
Amplification Refractory Mutation System – Polymerase Chain Reaction for Rapid Detection
of rpoB Gene Mutations in Mycobacterium tuberculosis International Journal of Applied
Science & Biotechnology 2017; Vol 5(1): 81-85
25. Evaluation of Flavonoid Content, Antibacterial,Antioxidant and Anticancerous Properties of
Six Medicinal Plants of Nepal. Deepak Raj Pant, SilaMahatara, UshaAdhikari ,smitashrestha
Journal of academic research,2017
2016
26. Prabin Shrestha, Bishnu Joshi, Jarina Joshi, RajaniMalla, Lakshmaiah Sreerama. Isolation
and Physicochemical Characterization of Laccase from Ganoderma lucidum-CDBT1 Isolated
from Its Native Habitat in Nepal. BioMed Research International Volume 2016 (2016), Article
ID 3238909, 10 pages
27. Panday R, Bhatt P S, Bhattarai T, Shakya K and Sreerama L. Aldehyde dehydrogenase
expression in Metaphire posthuma as a bio-indicator to monitor heavy metal pollution in
soil. BMC Res Notes. 9:491.
28. Bibek Bhatta, RoshinaThapa, Sanjay Shahi, YogeshBhatta, Dipendra Raj Pandeya, BalHariPoudel. A Pilot Study on Screening of BRCA1 Mutations (185delAG, 1294del40) in Nepalese Breast Cancer Patients. Asian Pacific Journal of Cancer Prevention, 2016. Vol 17,
29. Birendra Prasad Gupta, Thupten K Lama, AnuragAdhikari, Anant Shrestha,
RamanujRauniyar, BinaySapkota, Sandeep Thapa, Pawan Prasad Gupta, Krishna Das
Manandhar ; First report of HEV viremia in healthy blood donors from Nepal;Virus
Disease. 2016. doi:10.1007/s13337-016-0331-y.
30. Upadhya BP, Malla R, Manandhar KD, Gupta BP, Adhikari A, Rauniyar R, Tamarkar
CS, Jha BK, Kurmi R. Zika and Its Preparedness in Nepalese Scenario. SM Virol. 2016;
1(2): 1008
31
31. Katawal I., Kharel G.P., Poudel BH.Antioxidant Activity and Cytotoxicity of Nepalese Nak Cheese Journal: Biological and Chemical Research,2016. Volume 3, 234-245
32. Moonens, K.; Gideonsson, P.; Subedi, S.; Bugaytsova, J.; Romao, E.; Mendez, M.; Norden, J.;
Fallah, M.; Rakhimova, O.; Shevtsova, A.; Lahman, M.; Castaldo, G.; Brannstrom, K.;
Coppens, F.; Lo, A. W.; Ny, T.; Solnick, J.; Vandenbussche, G.; Oscarson, S.; Hammarstrom,
L.; Arnqvist, A.; Berg, D. E.; Muyldermans, S.; Boren, T.; Remaut, H. (2016). Structural
Insight in Adaptive and Redox-sensitive ABO Glycan Binding by Helicobacter pylori. Cell
Host & Microbe 2016 January 13; 19(1): 55-66.
2015
33. Gupta BP, Adhikari A, Rauniyar R, Kurmi R, Upadhya BP, Jha BK, Pandey B,
DasManandhar K; Dengue virus infection in a French traveller to the hilly region of
Nepal in 2015: a case report;BMC J Med Case Rep. DOI 10.1186/s13256-016-0847-1.
34. AnuragAdhikari, Birendra Prasad Gupta, Krishna Das Manandhar, Shravan Kumar
Mishra, Hari Krishna Saiju, RajendraMaan Shrestha, Nawneet Mishra and Shishir
Sharma; Negative feedback circuit for toll like receptor-8 activation in human
embryonic Kidney 293 using outer membrane vesicle delivered bi-specific siRNA;
BMC Immunology.2015. 16:42 DOI 10.1186/s12865-015-0109-9.
35. Singh S, Gupta BP, Manakkadan A, Manandhar KD, Sreekumar E;. Phylogenetic
study reveals co-circulation of Asian II and Cosmopolitan genotypes of Dengue virus
serotype 2 in Nepal during 2013; Infect Genet Evol. 2015. 34: 402–409
36. Adhikari A, Rauniyar R, Raut PP, Manandhar KD, Gupta BP, 2015.Evaluation of
sensitivity and specificity of ELISA against Widal test for typhoid diagnosis in endemic
population of Kathmandu. BMC Infectious Disease; 2015, 15:523.
37. Birendra P Gupta, Sneha Singh, RoshanKurmi, Rajani Malla, EaswaranSreekumar,
Krishna Das Manandhar. Re-emergence of dengue virus serotype 2 strains in a 2013
epidemic in Nepal. Ind J Med Res; 2015,142 (Supplement), pp 1-6. DOI:10.4103/0971-
5916.176564
38. Satya Prakash, Avishek Mishra, Tilak Ram Shrestha, NagendraAwasthi, Isha
Pradhan, Anushuman Mishra and Krishna Das ManandharInternational. Evolutionary
prospective of Indian Subcontinent Population. Techno Consultancy Services Journals.
2015, 4(1):124-129.
39. Bhim Prakash Khatri; Tribikram Bhattarai; Sangita Shrestha and Jyoti Maharjan.
Alkaline thermostable pectinase enzyme from Aspergillusniger strain MCAS2 isolated
from Manaslu Conservation Area, Gorkha, Nepal. Springer Plus.2015. 4:488.
40. BimalaDhakal and Jarina Joshi. Neutral Red Immoblized Graphite Felt Anodic Microbial Fuel Cell for Wastewater Treatment and Generation of Electricity. J. Bioprocess Bioprocessing Biotech, 2015.5(11):261 (http://dx.doi.org/10.4172/2155-9821.1000261).
32
2014
41. Manandhar Krishna Das, Yadav Thakur Prasad, Prajapati Vijay Kumar, Basukala
Om, Aganja Ram Prasad. Nanonization increases theantileishmanial efficacy of
amphotericin B: an ex vivo approach. Advances in experimental medicine and biology.
2014; 808 doi:10.1007/978-81-322-1774-9_7
42. Gupta BP*, Adhikari A, Chaudhary BK, Manandhar KD, Yadav SB, Shrestha N,
Chaudhary P, Rauniyar R, Patel BD. 2014. Hepatitis E outbreak in Nepal during 2014.
Journal of Advance in Biology. 5: 610-613
43. Bishnu Joshi, Basant Pant, Pravesh Rajbhandari, Ram Prasad Aganja, Rajani Malla,
Krishna Das Manandhar and Lakshmaiah Sreerama. The Epidemiology and Health
Burden of Neurocysticercosis in Nepal. International Journal of Tropical Diseases &
Health,2014.4(2): 204-223.
44. Gupta BP*, Mishra SK, Manandhar KD, Malla R, Tamarakar,CS, Bajarcharya A,
RauniyarR, Raut PP, Sah SK and Pokhrel S. Comparison of CPE NT and PRNT assays
for estimating Neutralizing antibody titres against Japanese Encephalitis Virus,
Int.J.Curr.Microbiol.App.Sci.2014.3(1): 407-413
45. Jarina Joshi, Rejeena Shrestha, RojlinaManandhar, Krishna Manandhar, LakshmaiyaSreerama, TribikramBhattarai.. Improvement of ethanol production by electrochemical redox combination of yeast cells. International Journal of Biological Sciences and Applications, American Association of Science and Technology. 2014.1(3), PP 44-51. (http://www.aascit.org/journal/ijbsa).
46. Baplu Rai, Ashish Shrestha, Shishir Sharma and Jarina Joshi. Screening, Optimization and Process Scale up for Pilot Scale Production of Lipase by Aspergillusniger. Biomedicine and Biotechnology. 2(3): 54-59 (pubs.sciepub.com/bb/2/3/3 © Science and Education Publishing DOI:10.12691/bb-2-3-3)
47. Ritambhara Aryal, Anil K. Sah, Deepak S. Paudel, Bishnu Joshi, Sunil P. Lekhak, ReemaRajbhandari, BalHariPoudel, Bijaya Pant, Ganesh Prasad Neupane, Basant Pant. Polymerase chain reaction using the mpb64 fragment for detection of mycobacterium tuberculosis complex DNA in suspected TB cases. International Journal of Medical Science and Public Health. 2014. Vol 3. Issue 11.
48. RanjanKoirala, Giovanni Ricci, Valentina Taverniti, Chiara Ferrario,RajaniMalla,
Sangita Shrestha, Maria GraziaFortina, Simone Guglielmetti.Isolation and molecular
characterization of lactobacilli from traditional fermented Dahi produced at different
altitudes in Nepal. Dairy Sci. & Technol. Vol. 94:397–408 DOI 10.1007/s13594-014-
0167-4, 2014
2013
49. Chhipi Shrestha, J. K.,.Bhattarai, T., Sijapati, J., Rana, N., Maharjan1, J., Rawal D. S.,
Raskoti B. B., Shrestha S. Assessment of Genetic Diversity in Nepalese Populations
of Swertiachirayita (Roxb. Ex Fleming) H. Karst Using RAPD-PCR
Technique. American Journal of Plant Sciences, 2013. 4: 1617-1628.
33
2012
50. Geroge P., Timothy D. Smith, Heather Howard, Fahd Al-Mulla, LotfiChouchane,
AndreaseHadjisavvas, Tilak Ram Shrestha et al. Human Variome Project Country
Nodes: Documenting Genetic Information within a Country. Human Mutation. 2012.
33(11):1513-1519.
51. Buddha Bahadur Basnet1, Til Bahadur Basnet, Bishnu Joshi, Rajan Kumar Dahal,
Rajani Malla. Multidrug resistance Acenetobacterbaumannii: A systematic review for
microbial and clinical study. International journal for applied biology and
pharmaceutical technology. 4 (2),166-184, 2012.
2011
52. Bishnu Joshi, Govind Prasad Shah, Buddha Bahadur Basnet, Meg Raj Bhatt, Dinita
Sharma, Krishna Subedi, Janardhan Pandey and Rajani Malla. Phytochemical
extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum
(Tulsi),Eugeniacaryophyllata (clove), Achyranthesbidentata (Datiwan) and
Azadirachtaindica (Neem). Journal of microbiology and antimicrobials. Vol. 3 (1):1-7,
2011
53. Bishnu Joshi, Megh Raj Bhatt, Dinita Sharma, Jarina Joshi, Rajani Malla and LakshmaiahSreerama. Lignocellulosic ethanol production: Current practices and recent developments. Biotechnology and Molecular Biology Review, 2011, Vol 6(8), 172-182.Available online at http://www.academicjournals.org/BMBR
2010
54. R. Maurya , R. Kumar , V. K. Prajapati , K. D. Manandhar , D. Sacks , S. Sundar& S.
Nylén. 2010. Human visceral leishmaniasis is not associated with expansion or
accumulation of Foxp3+CD4 cells in blood or spleen. Parasite Immunology.
2010.32(7): 479 – 483.
2009
55. Pant, D.R., Bhattarai T., Beck, E and Fettig, S. Genetic transformation of Nepalese
wheat (Triticumaestivum,L) cultivars with ipt gene under the regulation of a senescence
induced promoter from maize. Pak. J. Biol. Sci. 2009. 12(2):101-109
Biotechnology Products in Country Development
Students engaged in different fields (nationally/internationally)
Fields Numbers of students
Ph. D. USA 22
Ph.D. other countries/ Nepal 16
Civil services/ Government
institute
9
Research Lab 28
34
Teaching 10
Miscellaneous (thesis
remaining + dormant)
36
Besides, several students have owned their one biotech-business and benefitting themselves
and providing job opportunities for others.
1. Arya Diagnostics (Bagbazar, Kathmandu): molecular disease diagnosis (Raju Lama)
2. Dirghayu Biotech (Godawari, Lalitpur): use of molecular tools in new innovations
(Mitesh Shrestha, Rajendra Napit, Roshan Nepal, Sujindra Subedi).
3. Praramva Biotech (Sitapaila, Kathmandu): biofertilizer and biopesticide production
(Baidyanath Jha)
4. Ficus Biotech (Dhulikhel, Kavre): tissue culture propagation and distribution of
Paulownia, Bamboo plants (Santosh Dahal)
5. Rara Biotech: Diagnostic kit development.
6. Sumeena Karki, Bimala Dhakal, Gauri Thapa
Conferences/Workshops/Schools (2017 – 2020)
International
1. "International Workshop on Advancing Biological and Clinical Research with
Flow cytometry-2019" on March 2-6, 2019.
2. Himalayan Cysticercosis International Conference (HCC) 2018 [Co-
organizer]. December 7-8, 2018
3. International Workshop on Application of Flow Cytometry in Biotechnology-
2017, on September 14-17, 2017
4. 3rd
international conference “South Asian Biotechnology Conference -2017”-
March 16-18, 2017)
National
1. IInd
CDBT-TU Winter School-2020 on Experimental Biotechnology: A
Teachers’ Training (Hands on training) on January 27-31, 2020
2. First ELOQUENCE and Academia-Industry Interaction program 2019.
3. CDBT-TU Winter School-2018 on Applied Molecular Biology (Hands on
training) on January 7-10, 2018
4. First Biotech - TU Summer School - 2017 on Applied Molecular Biology, on
April 06-09, 2017
5. Workshop on Bio-prospecting of Nepalese medicinal plants and Drug
Development, 2017
35
36
Foreign delegates: Flow cytometry workshop - 2018
Participants after "International Workshop on Advancing Biological and Clinical
Research with Flow cytometry-2019" on March 2-6, 2019.
Workshop and Seminar
37
Three-Day Workshop on "Screening of Natural Products (microbes and plants)
and their anti-infective activities" and Interdisciplinary Seminar on "Natural
Products and Drug Discovery"
Four-Day Workshop on “Molecular Biology on Hand Training“ -2017
38
Winter School -2018
Eloquence -2018
39
Winter School – 2020 [Experimental Biotechnology: A Teachers’ Training ]
A Day Seminar on Viral Diseases
Scientific and Academic Visitors, faculties and scientists
40
Scientific and Academic Visitors, faculties and scientists
41
Scientific delegates in international conference organized by Department
42
Talk with Prof. Poul, NIH 2019
Group Photo after talk with VC from Sikkim University, India- 2019
43
List of some Visitors (Scientists/Researchers)
1. Prof. Paul K. Wallace Roswell Park Cancer Institute, USA
2. Dr. William Telford Aational Cancer Institute, NIH, USA
3. Dr. Sujan Shrestha La Jolla Institute for Allergy and Immunology, USA
4. Dr. Annie Elong Ngono La Jolla Institute for Allergy and Immunology, USA
5. Dr. Melanie M'cCauley University of California San Diego, USA
6. Prof. K.R.S Sambasiva Rao Mizoram University, Mizoram, India
7. Mr. Matthew Ellenbogen La Jolla Institute for Allergy and Immunology, USA
8. Ms. Jenna Hambrick La Jolla Institute for Allergy and Immunology, USA
9. Dr. Rekha Gaur Tata Memorial Center, India
10. Dr. Hemant Agrawal Flow Cytometry Solution, India
11. Dr. David Hedley University of Toronto, Canada
12. Ms. Sue Chaw University of Toronto, Canada
13. Prof. Brook Milligan New Mexico State University, USA
14. Prof. Christian Koch Friedrich Alexander Universitat, Germany
15. Prof. Alok Srivastav Mumbai, India
16. Prof. Mamoru Yamada Yamaguchi University, Japan
17. Dr. Yogan Khatri University of Illinois at Urbana-Champaign.
18. Dr. Prabhat S. Kunwar Biogen Inc., USA.
19. Prof. Jing Che Kunming Institute of Zoology, CAS, China
20. Prof. Dr. Christian Koch Friedrich-Alexander Universitat Erlangen-Nurnberg, Germany
21. Prof. Dr. Jyoti Prakash Tamang Sikkim University, Gangtok, Sikkim, India (Acting VC)
22. Prof. Leah Gheber Ben-Gurion University of the Negev, Israel
23. Prof. Livio Tvainotti University of Padova, Padova, Italy
24. Dr.BishesPoudel/Dr.Sampurna Civil Hospital, Baneshwor
National and International Collaborations
This department has established Collaboration with different institutes and regularly
collaborating research work with the institutes.
Table 11: List of MOU with different National and International Institutes:
S.N Title Start Date End Date Remarks
1 Alpha Agro. Pvt. Ltd. January 12, 2020 Unlimited
2 Medi Quest Laboratory Clinic P. January 12, 2020 Unlimited
44
Ltd. (Magh 6, 2076)
3 Natural History Museum,
Tribhuvan University,
Swayambhu, Kathmandu
June 1st , 2019
(Jestha 18, 2076)
June 1st, 2022
4 State Key Laboratory of Genetic
Resources and Evolution,
Kunming Institute of Zoology,
Chinese Academy of Sciences,
Kunming, China
13th
August,
2019
13th
August,
2023 (4 years)
5 Sukraraj Tropical and Infectious
Disease Hospital, Teku
18 December,
2018
2022
6 National Public Health
Laboratory (NPHL), Department
of Health Services (DoHS), Teku,
Kathmandu, Nepal.
29th
Dec 2014
Unlimited
7 Muscular Dystrophy Foundation,
Nepal.
17 August
2012
Unlimited
8 Department of Food Technology
and Quality Control (DFTQC),
Babarmahal, Kathmandu, Nepal.
07/05/2066 10 Years after
the date of
signature
Ended
9 Rastria Suntalajat Bali Bikash
Karyakram, Kirtipur, Krishi
Bibhag.
25/11/2067
One year Ended
10 St. Cloud State University, MN,
USA.
April 4, 2011 Unlimited
11 Rajiv Gandhi Centre for
Biotechnology (RGCB),
Thiruvananthapuram, India.
15 Jan 2014 3 years Ended
12 La Jolla Institute for Allergy and
Immunology, San Diego, and
KARIUS Inc. San Fransisco,
California, USA.
Unlimited
13 Centre for Health and Disease
Studies- Nepal (CHDS-Nepal),
Kathmandu, Nepal.
Unlimited
14 Karius, Inc. 1505A Adams Court,
Menlo Park, CA 94025.
Unlimited
15 The Western Australian
Neuroscience Research Institute.
28th
October
2016
4 Years
16 Hester Biosciences Nepal Private
Limited, Nala Sera, Ugrachandi
VDC-7, Kavre, Nepal.
15/10/2015 3 Years from
the date of sign
Ended
17 Mega Agrotech Pvt. Ltd. 06/03/2067 10 Years
18 Apeejay Stya University
Sohna -Gurgaon, NCR India
31st March 2015 3 Years Ended
19 National Ayurveda Research and
Training Center(NARTC),
Government of Nepal, Kirtipur
July, 2018 Unlimited
20 Institute de Rechurche pour le
Development (IRD)- France
45
21 University of Zurich
Faculty and students in International universities/research centers
Institute where Faculties doing Ph. D. and M.Sc. students participated in Conference and
short scientific visits from department presented papers and got best awards
Recognitions
Science Awards
46
a. Nepal Academy of Science and Technology Award [Nabil Science and Technology
Award] in 2017 and 2018 to Prof. Krishna Das Manandhar and Prof. Rajani Malla
b. Best poster award from ICGEB-Italy to a student in 2018
c. Seventh batch student Ms. Srijan Shrestha supervised by Prof. Krishna Das
Manandhar was awarded in the international workshop organized by ICGEB,
Trieste-Italy got the Best Poster Award on the research work presented in the topic
“Immuno-molecular study of cutaneous leishmaniasis: an alarming and emerging
disease of Nepal”
d. Dayananda Bajracharya Best Thesis award of NAST - 2019
Sixth batch student Mr. Mahesh Lamshal supervised by Prof. Krishna Das
Manandhar got the award of the Best Master Thesis titled on “Development and
Evaluation of an in-House IgG-Capture ELISA for the Detection of Dengue virus “
nominated by Nepal Academy of Science and Technology.
Industry.
Recognition of research product by Industry - 2019
• Alpha Agro Pvt. Ltd., Birganj has recognized our thesis research supervised by Dr.
Pramod Aryal and 4 of our graduates who worked on “Biofertilizers” are instantly
recruited as researcher in the company.
• The Biopesticide produced by the graduates became successful to control ARMY
WORM which has been a chronic issue for the farmers due to devastating damage of
crops
.
47
Nepal Academy of Science awards to Prof. Krishna Manandhar, Prof. Rajani Malla
and Student Roshan Nepal
Students receiving toper (Mr. Rajindra Napit) and best thesis (Ms. Sthapit) awards.
IT supports
48
The Department has 25 desktop computers and 12 laptops installed in different teaching-
learning rooms and provided to the faculties. The facilities have been found beneficial to the
Department and faculties.
Table 10: Laptop and computers available in department
S. N Number of Computer Remarks
1. Bioinformatics Lab 12
2. Faculty 10 Laptop
3. Computer /library 1
4. Store 1
5. Account 1
6. HOD Office 1 + 1 all in one PC + 1 laptop
7. Instruments 6 + 1 laptop
8. Lab Assistant 1
9. Class room 1
Faculties with laptop handed over by department
The administrative and library function has been regulated by the Education Management
Information System (EMIS) developed as per need of the Department. The system is
functional and has been easy to get required information instantly.
49
EMIS developed for the analysis of administrative data of the Department
Student support
Library
50
The Library of the Central Department of Biotechnology has approximately a collection of
614 volumes best reference books on biotechnology. In addition, Department has regular
provision of deposition of masters’, Ph. D. students thesis and research projects. The
students and faculty have access to use Tribhuvan University central library. Central
Department has access for different national and international online journals.
Journals/Periodicals subscribed by the library Journals available in Central Department of
Biotechnology library
Fig 18: Library in the department
National
RevoScience (Half yearly Journal)
Nepal Chemical Society (Annual Journal)
National Academy of Science and Technology Journal (Annual Journal)
International Journals and Journals in the process of ordering
Current trends in Biotechnology and Pharmacy (INDIA)
Applied Microbiol. Biotechnology
Indian Journal of Biotechnology
Trends Biotechnology
Nucleic acid research
Yeast
Scientific journal of Biotechnology
E- Journal access in Central Library, Tribhuvan University
1. American Institute of Physics journals
2. Annual Reviews
3. OUP - Oxford Journals
4. Project MUSE journals
5. Nepal Journals Online (NepJOL) www.nepjol.info
List of Online Journals (NepJOL)
3 Agronomy Journal of Nepal
5 Asia Pacific Journal of Environment Ecology and Sustainable Development
51
31 International Journal of Applied Science and Biotechnology
32 international Journal of Environment
43 Journal of Biomedical Science
49 Journal of Food Science and Technology Nepal
54 Journal of institute of Science and Technolgy
62 Journal of Nepal Agriculture Research Council
63 Journal of Nepal Chemical Science
67 Journal of Nepal Pharmaceutical Association
79 Journal of The institute of Agriculture and animal Science
83 Journal of Universal College of Medical Science
85 Kathmandu University Journal of Science, Engineering and Technology
86 Kathmandu University Medical Journal
89 Nepal Agriculture Research Journal
90 Nepal Journal of Biotechnology
94 Nepal Journal of Medical Science
97 Nepal Journal of Science and Technology
100 Nepalese Hart Journal
101 Nepalese Journal of Biosciences
111 Our Nature
114 SAARC Journals of Tuberculosis, Lung Diseases and HIV/AIDs
122 Tribhuvan University Journals
125 Water Nepal
Other Facilities
Besides this department has facilities of shearing Tribhuvan University properties. Students,
staffs and faculties are getting the facilities every year.
Tribhuvan University Central Library is located about 100 meter away from existing
department. Plenty of books related to biotechnology are available there. List of
books related to biotechnology available in Central library are listed in
appendix.Besides online journal facility is also available in Central library.
A sheared playing ground is available in front of department where students and
faculties can play outdoor games. Besides, Tribhuvan University has plenty of free
spaces which can be utilized freely, as this department is constituent to Tribhuvan
University]
Central Hostel of Tribhuvan University is located about 500 meter away from
existing Department. However, very few students of biotechnology are staying in the
hostel
Central TU press and Guest house is present in the TU campus premises.
There is a central health facility in the kirtipur campus where students and staffs can
go for health check up.
52
53
Different facilities available to the department by Tribhuvan University
Extra Curricular Activities
54
55
Components of University
Departmental organizational set up.
56
Curriculum Development and instruction
Central Department of Biotechnology was established in 2007 AD under Institute of Science
and Technology, Tribhuvan University, Nepal. Tribhuvan University is the premier
university in Nepal, which provides courses in diverse disciplines at various levels of
modern biology. Considering the potentialities of modern biotechnology, TU established
Central Department of Biotechnology hoping for a leading role in fulfilling the national
demand on the manpower and also to start innovative researches in various fields of
biotechnology. The curriculum of Master level in biotechnology has been formulated
considering the need of the current nation’s requirement as well as to meet the international
standard in the subject. It is a technological professional manpower preparation program as
of other Institute of engineering, Institute of agriculture & animal Science, Institute of
Medicine and Institute of Forestry programs of Tribhuvan University.
Department is in the process of curriculum revision. It has send letters to the experts for 2nd
phase suggestion. Recently a close meeting was held along with experts to get comments on
curriculum. Two workshops held at three years interval time (2010/11 and 2014/15) and
those meetings amended the curriculum and forwarded to Academic Council, TU for
approval. As this department was new, there were plenty of flaws in the first time when the
department was formed, those pitfalls were corrected. Now and then faculty meeting are
conducted along with the national and international experts of Biotechnology in Nepal to
evaluate suitability of the curriculum so that true matching of academic goals and objectives
with teaching is judged.
Curriculum are showing positive effects for students learning. Those positive effects are
being noticed specially in students final examination, proposal defense, final thesis defense
and paper presentation outside the University. For strengthening of students’ confidence, 2
non-credit courses and several elective courses are introduced in the curriculum and students
are given freedom to choose elective courses offered by department. No inferior report on
students’ performance is received from any of the scientists or expert under whom the
students worked. Department has sufficient evidence of the possession of international
qualities of the students both in theory and practical field. All students have ability to run use
bioinformatics lab with access of computer and internet facility.
This department encounters some dilemma now and then with regard to the curriculum
development: whether it should focus its curriculum for training students for the advanced
studies in abroad or it should train the students to make able to address national issues. If it
57
focuses to the latter, the problem is that there is less employment opportunities in the field of
biotechnology in Nepal. Hence this scenario urges for immediate increment of employment
opportunities in the field of biotechnology of Nepal so that graduates of this department
would be fully utilized within the country.
Specific priorities area
1. Tropical Infectious and vector born Diseases diagnosis
2. Viral Disease molecular characterization and development of diagnostic kits/vaccine
candidate molecules
3. Immune responses of Nepalese population against infections
4. Bacteriophage as control of MDR bacterial infection
5. Biofertilizers and Biopesticides
6. Bioactive compound production from microorganisms like Streptomyces
7. Bioprospecting of the medicinal plants
8. In vitro fertilization in animal models to large animals
9. Bio-fuel engineering
10. Plant and Fungal culture
Hugely expecting Major instruments in 2020
With support from QAA
Higher Education Reform Project called application from QAA certified academic
institution for Resource Management fund. The applicant sought one part of investment from
its own resources for which two part of the budget will be supported by UGC-Nepal. Central
Department of Biotechnology with its available fund resources (10 million) planned to
propose 3o0 million procurement plan in which it plans for hugely required Next Generation
Sequencer (NGS) with bioanalyzer, HPLC, Ultra centrifuge, compound microscope, biosfety
cabinet etc as mentioned in the table below. Besides it has applied for laboratory
strengthening grant support of equivalent amount 5 million Nepali currency and scheduled to
purchase Ultra Centrifuge machine. Department envisages a well equipped advance
laboratory after the completion of the grant utilization.
58
Procurement Plan under Higher Education Reform Project -2020
59
Ultra centrifuge HPLC
Next Generation Sequencing
Hugely expecting Major instruments in 2020
Advancing the Laboratory with highly sophisticated equipments with support from QAA
60
Dream Biotechnology Complex
On going construction Building Site
On going construction Building Plan
61
Current construction Building Plan
Building Sketch
62
Strengths Learnings Opportunities and Challenges (SLOC) analysis
STRENGTH
1. High demand of the course (Applicant 4/5 times higher than capacity, Skill oriented
course, Coverage of diversity of knowledge)
2. Well established laboratory (Few advance equipments and all functional, All M.Sc.
based research works doable)
3. Twenty four hour working environment (Solar backup, water supply)
4. National and International research grant are on going
5. Many international scientists, researchers, faculties visit frequently
6. Vibrant faculties (Every Faculty has grants).
7. Capability to conduct national and international seminars/conferences/workshops/
training programs.
8. Research conductive environment.
9. Viable academia to industry approach
10. New building with enough space to expand
LEARNINGS
1. Very applied science in the context of Nepal.
2. Huge expenses in running the research and course.
3. Long run research grants are required to keep laboratory sustained.
4. Qualified and dedicated faculties and staffs are required.
5. Regular visits of scientists/researcher are sought.
6. National and international networking is must.
7. Honesty and sincerity in the students makes possible to run lab day-night.
8. Rooms of scope for biotechnology research in Nepal.
OPPORTUNITIES
1. Agriculture: Masters’ students have to take 3 credit course on Agriculture
biotechnology with 1 credit practical. This makes the student suitable to work in
agriculture sector. The syllabus includes mushroom culture, vermicompost, tissue
culture etc.
63
2. Medical: The syllabus includes 3 credit hour course on Medical biotechnology with
1 credit practical. This makes the student suitable to work in medical sector. The
syllabus includes disease diagnosis, immunology, vaccine production etc.
3. Environmental: After completion of Masters’ degree, students will be able to work
on different environment clinic techniques and use of waste to convert into value
added products.
4. Development: Stdents will be able to think for establishment of own industries.
5. Beautification: Masters’ students will be able to work on plant tissue culture so that
required plant seedling coul be cultured.
CHALLENGES
1. Number of senior faculties are retiring and new competent faculties through regular
and fast track to be recruited
2. Completion of its own building.
3. Initiation of new courses like M.Sc. Virology, Bioinformatics etc
4. Country base research identification and search of funds.
5. International grants to sustain the research activities
6. Sustainability of the Departmental strengths
Financial sustainability
The Department draws on various sources of revenues mentioned above, allowing it to
support its ongoing efforts and to undertake new initiatives. The flow chart shows how all of
these streams of sustainability will be exercised in the Department. The strategic plan is the
anchor, in which an organization’s programs, structure and systems, as well as financials are
reviewed and new opportunities are identified.
The new program opportunities are pursued using a distinct resource mobilization strategy.
All of these instruments are designed to showcase Department’s programs, institutional
structure, and financial health.
64
Rules and regulations
Tribhuvan University is an autonomous academic institute which develops the rules and
regulations independently within its vision “Tribhuvan University, as a credible central
university with a higher rank in the global arena, will be recognized for quality education
blended with research, innovation, knowledge creation, and transformative teaching and
learning at an affordable cost ensuring access and diversity through effective and efficient
utilization of its human and physical resources”. The rules and regulations approved by the
Executive Board of the university is being followed exactly by the Department in all
academic, administrative, economic and social works.
However this department has its own autonomous rules to conduct everyday works. All
executive works in the department is conducted under the leadership of Department head.
Overall, the department itself is under Tribhuvan University norms and policies and has its
own organizational structure which runs under Vice chancellor, Registrar, Rector and Dean-
Institute of Science and Technology. The activities in the Department are being carried out
forming a suitable organizational structure/committee as required. The detailed rules and
regulation required while working at the Department level will be formulated by the
Management Committee or Department Meetings which will be implemented only after
approval from the TU authorities.
Recruiting human resources
For recruiting permanent teaching staff and non-teaching staff, procedure adopted by
Service Commission, Tribhuvan University is followed.
Vacancy is announced in national newspaper which is later followed by written test
and interview. Generally, minimum requirement for eligibility of any teaching staff is
master’s degree in biotechnology or related subjects.
For contract post of teaching and non-teaching staff, vacancy is announced in national
newspaper and applicants are interviewed for selection.
For non-teaching staff, vacancies for the required post will be recruited based on
defined qualification according to Tribhuvan University rules.
Department has right to manage some academic programs as per need such as:
Entertaining visiting faculty: Department can hire visiting/part-time faculties but has to
get approval from center office. Under the Fulbright Program, three full time visiting
65
faculties/experts (Prof Dr. Lakshmaiah Sreerama, Prof. Alanka Hozaicand and Dr.
Melanie McCauley) were recruited under the leadership of HOD.
Organizing seminar and conferences: Pertaining to the Departmental academic programs,
it can organize activities without in notification to the TU authorities. Several national
and international seminars/conferences has been organized without prior information to
Tribhuvan University authority.
Running projects: Department can apply and receive research grants, however, it has to
inform to the TU center office. Several faculty grants research projects were completed
and more are running in the Department. For applying a grant, faculty and students can
get approval from Head of Department.
Financial mobilization
Department is run as of Self-Sustained Program. University provides the salary of its
permanent teaching and non-teaching staffs. Remaining all other expenses are to be cover
wither by the students’ fee or by research projects. Besides, university provides
Development Funds which has to spend as per the defined job of Tribhuvan University.
Department need to request optimum budget required for the expenses in different
categories beforehand the fiscal year starts for all expenses it incurs.
There is need of financial audits from TU level and Government Level every year.
a. Governance mechanism
The Department is led by Head of the Department, however, it runs in the direction provided
by the management committee. For the support to run the daily issues of the Department, it
has formed many committees.
Management committee: The committee led by Head of the Department comprises at least 5
members which advices the HOD to manages the daily routine work of the Department. The
team members are represented by the heads of account, administration office and faculties.
Subject committee: There is a full body subject committee comprising maximum 21
members from Department faculties, experts, ex-faculties and faculties from other TU
institutions which is chaired by HOD. The committee has responsibility to look after course
revision, new courses, equivalency issues required for the Department. Among the team,
66
there is provision of Executive Subject Committee led by HOD comprising at least 3
members which take care the frequent issues which really do not need full body consensus.
Research committee: All the research related activities of the Department is managed by this
committee which also has provision of chair by HOD comprising at least 3 members among
the Department faculties. It looks after the activities of M.Sc. projects and thesis, Ph.D.
Thesis, national and international projects, research ethical issues etc.
Entrance/Examination committee: A faculty nominated by the HOD will function as chief of
the committee and conduct any kind of examination especially the entrance exam.
Table 12: Different working committees formed in the department
SN Name of the Committee
1 Management committee
2 Subject committee
3 Research committee
4 Entrance examination committee
5 Internal Quality Assurance Committee (IQAC)
6 EMIS Committee
7 Purchase Committee
8 Nepal Biotechnology Society
67
SUMMARY
List of Achievement/Impact of QAA
1. Science Awards
a. Nepal Academy of Science and Technology Award [Nabil Science and
Technology Award] - 2017 and 2018 [Prof. Krishna Das Manandhar and
Prof. Rajani Malla]
b. Best poster award from ICGEB-Italy to a student - 2018
c. Krishna Chandra Best Thesis award to a student – 2018
d. Dayananda Bajracharya Best Thesis award of NAST – 2019
e. International conference awards (Microbiology conference) - 2020
f. Research Grants
g. ICGEB International Collaborative Research Programme Grant- Italy 2020
h. LJI-KARIUS Grant –USA 2016 – 2020
i. Epstein Barr Virus Pilot project -2019 -2020
j. Every Faculty has research project fund
2. There are three international projects and 7 national project and all the faculties
do have either national or international grants for their research projects.
3. Industry [Recognition of research product by Industry]
a. Biofertilizer
b. Biopesticide
c. Classical swine virus molecular characterization
4. Internationalization
a. Fulbright specialist program
b. Humbolt research scientist
c. Research scholar at NIH
5. Attraction for other streams
a. MBBS-MD to the PhD course
b. Medical Laboratory Technology to M.Sc /PhD.
c. Microbiology/Botany/Zoology/ Vet. Science
6. Procurement Plan for purchasing advanced equipment
a. Next Generation sequencer,
b. Bioanalyzer,
c. HPLC
d. -800C Deepfreezer etc.
7. Model Department for training to university teachers, students, professionals on
biotechnology tools
• Winter schools
• Summer Schools
• Workshops (National and Internationals)
8. Students are regularly involved in indoor and outdoor sports activities.
• Karante
• Footsal
• Cricket
• Vollyball etc.
68
Student Strenth
Summary of students flow in the department
Batch Enrollme
nt Year No. of
M.Sc.
Students
appeared in
Final Exam
Distinction First
Divisi
on
Second
Division Thesis/
Transcript
Pending /
Remarks
1st
Batch 2009 22 7 15 0 0
2nd
Batch 2010 23 13 9 1 0
3rd
Batch 2011 21 9 12 0 0
4th
Batch 2013 22 9 12 1 0
5th
Batch 2014 20 6 11 3 0
6th
Batch 2015 20 5 15 0 0
7th
Batch 2016 21 5 15 1 0
8th
Batch 2017 24 1 3 0 20
9th
Batch 2018 22 Ongoing
10th
Batch 2019 24 Ongoing
11th
Batch 2020 24 Under joining
0
5
10
15
20
1st 2nd 3rd 4th 5th 6th 7th 8th
No
. of
stu
de
nts
Batch of Students
Students Profile of different batches of students enrolled
Male
Female
Left after admission
Janajati Male
Janajati female
69
List of thesis title provided to students currently in the year 2020
S N Thesis Title
1 Competitiveness of different serotypes of dengue viruses and genotypes of JEV in vitro
(c6/36, BHK, Vero) and in vivo (mosquito, baby chicks).
2 Study of T cell subset population as host Immune Response in Leishmania infection
3 Development and optimization of low-cost LAMP-based assay for the detection of
dengue virus
1st 2nd 3rd 4th 5th 6th 7th 8th
Nu
mb
er
of
stu
de
nts
Batch of Students
Placewise distribution of students
From kathmandu valley Out of Kathmandu valley
1st 2nd 3rd 4th 5th
Nu
mb
er
of
stu
de
nts
Batches of students
Result distribution of different batches of students
Distinction First Division Second division
70
4 Identification of pathogen specific bacteriophage from sewage sample and utilization of
phage to develop highly specific diagnostic tool using same phage for rapid diagnosis.
5 Purification of antimicrobials produced by Streptomyces coelicolor by co-feeding
different precursor: CysG riboswitch modulation as mechanism.
6 Riboswitch mediated inhibition: A novel approach towards antimicrobials production.
7 Treatment of kitchen waste by using microbial fuel cell.
8 Generation of ethanol using cheaper electrodes in electrochemical cell.
9 Use of pectinase and amylase producing strains for degradation of potato peels in
electrochemical cell.
10 Optimisation of copy number detection and ELISA of Hepatitis B Surface Antigen
Negative patients.
11 Genotype detection and cell viability testing by MTT assay
12 Detection of H25L mutation in Hepatitis B patients by PCR
13 Application of Lytic bacteriophage against MDR bacteria causing urinary tract
infections
14 Enhanced proton and electron generation through water splitting to augment CO2
reduction in dark through engineered RuBisCo enzyme.
15 Identifying the pathways for anti-cancer properties of natural plant extracts rich in
flavonoid compounds
16
Cloning of CobA protein of Salmonella typhimurium and purification of protein for
enzymatic activity inhibition in elucidating probable inhibitory effect of antimicrobial(s)
developed in Central Department of Biotechnology by S. coelicolar.
17 Cloning of Dam protein of Salmonella typhimurium and purification of protein for
enzymatic activity inhibition in search of SAM analogue as antimicrobial agent.
18
Molecular charaterization of plant pathogen and control (plant tissue culture model and
field trial). Focus on Coffee Leaf Rust, Fall Army Worm and Dadhuwa disease of L.
cardamom.
19 Use of nano-biotechnology for atmospheric CO2 reduction in dark and electro
biocemical synthesis of ethanol and polymers.
71
20 Micro-nano cellulose biosynthesis through genetic cloning from reduced CO2 as above.
21 Effect of bioactive phytocompounds in primary fibroblast cell
22 Collection of Cordyceps spp. from high hills of Kathmandu Valley and investigation on
their in vitro culturability
23 Molecular Detection of chikungunya virus in Nepal.
24 Evaluation of plant derived antidiabetic product by in vivo method.
25 DNA barcoding of High value Medicinal plants or Exploration of beneficial
microorganisms for industrial application
26 Molecular characterization of Hepetofauna in Ilam, Kathamndu valley and Panchase hill
area (Kosi Parbat and Syngja)
27 Effect of diluents and freezing techniques on post-thawed buck semen quality
Team Togetherness for the Department