Gene expression and function analysis

sample and assay technologies by QIAGEN

Enjoy first-time success

Rely on QIAGEN’s manual and automated workflow solutions for:

■ Sample collection and disruption

■ RNA stabilization and purification

■ Real-time PCR and RT-PCR and gene expression assays

■ RNAi and gene silencing

■ miRNA purification and assays

■ Methylation analysis in epigenetics research

■ Protein sample preparation and assays

Making improvements in life possible — www.qiagen.com

Sample & Assay Technologies

GEFPortf0

309S1W

W

“It’s truly exhilarating tobe recognized for yourhard work. This prizewill motivate younggenerations of scientistsand encourage newdiscoveries.“

Submission

deadline

August 1

imagination at work

For the purpose of this prize, molecular biology isdefined as “that part of biology which attempts tointerpret biological events in terms of the physico-chemical properties of molecules in a cell”.

(McGraw-Hill Dictionary of Scientific andTechnical Terms, 4th Edition).

GE Healthcare Bio-Sciences AB, a GeneralElectric Company.Björkgatan 30, 751 84 Uppsala, Sweden.© 2009 General Electric Company– All rights reserved.

The GE & Science Prize for Young Life Scientists.

Rewarding brilliance since 1995.

Imagine standing on the podium at the Grand Hotel in Stockholm, making youracceptance speech. Imagine joining the ranks of those published in Science magazineand having your essay on your work in molecular biology read by your peers aroundthe world. Imagine taking part in a seminar with the other Prize winners and NobelPrize laureates and discussing your work with leaders in the field. Imagine what youcould do with the US$25,000 prize money. Imagine this brilliant start to your careerand where it could lead you. Now stop imagining, and make it a reality.

Dr. Christine Jacobs-Wagner did just that. In 1997, she entered in the GE & SciencePrize for Young Life Scientists and won the grand prize. So why not you? If you are oneof those who were awarded a Ph.D in 2008, then submit your 1000-word essay byAugust 1, 2009. Your brilliant idea could well be on its way to building a better reality,for you and for those around you.

All the information you need awaits you at: www.gelifesciences.com/science

Dr. Christine Jacobs-Wagner

Grand Prize winner 1997

GE Healthcare

Coordinate your application focus.Achieve greater operational efficiency.GE Healthcare is focused on helping you develop efficient biopharmaceutical

process flows and eliminating wasteful practices, taking your value stream to

new frontiers. Through our wide range of technology platforms and services,

upstream and downstream from process development to final manufacturing

scale, GE Healthcare can connect you to the power of operational excellence.

Learn more about operational excellence at: www.gelifesciences.com/opex

GE Healthcare Bio-Sciences AB, a General Electric CompanyBjörkgatan 30, 751 84 Uppsala, Sweden© 2008 General Electric Company – All rights reserved.

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Your local distributor www.eppendorf.com/worldwide · Application Support E-Mail: support@eppendorf.com

Eppendorf AG · Hamburg · Germany · Tel: +49 40 538 01-0 · Eppendorf North America, Inc. · USA · Tel.: +1 800 645 3050

Each of your valuable samples deserves the best

treatment. See for yourself how the Eppendorf tips

will save time and reduce costs.

With respect to material, �t, design and operating forces

our tips set new standards. The close environment of

each sample should be adapted to its speci�c quality and

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absence of certain substances, but also stability, reliability,

or geometry. The Eppendorf tips are designed to cover all

of the speci�c needs of your samples!

Stop Aerosols! Eppendorf ep Dual�lter T.I.P.S.®

l Maximum protection of pipette and sample by our

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www.cellsignal.comfor quality products you can trust...

Antibodies and Related Reagents for Signal Transduction Research

Confocal immunofluorescent analysis of HeLa cells, treated with either 10 µM MG132 (left) or10 µM MG132 and 1 mM DMOG (right), using Hydroxy-HIF-1α (Pro564) (D43B5) RabbitmAb #3434 (green). Actin filaments have been labeled using DY-554 phalloidin (red).

The Highest Quality Antibodies for the Study of

Angiogenesis...from Cell Signaling Technology®

:: Cell Signaling Technology’s innovativeproducts offer unsurpassed sensitivity,specificity and performance.

:: Extensive in-house validation means thatoptimization is not left up to you, the user.

:: Technical support provided by the samescientists who produce and validate theproducts translates into a thorough, fastand accurate response.

Unparalleled product quality, validation and technical support.

Immunohistochemical analysis of paraffin-embeddedhuman astrocytoma using VEGF Receptor 2 (55B11)Rabbit mAb #2479.

Immunohistochemical analysis of paraffin-embedded mousesmall intestine using MMP7 (D4H5) Rabbit mAb #3801.

©2009

CellS

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ESSENTIAL BY NATURE

The 2009|10 NEB Catalog & Technical Reference

www.neb.com

Highlights

High Fidelity (HF) Restriction Enzymes - the next generation•

in restriction enzyme technology

A comprehensive offering of PCR reagents•

The latest innovation in competent cells for protein expression•

An extensive selection of markers and ladders for DNA,•

RNA and protein

A new gene expression and cellular analysis section showcasing•

a powerful protein labeling technology unique to NEB

A range of expression systems, including a novel kit for•

cell-free expression

Tools for glycobiology•

Tools to enable RNA research•

A substantial technical reference section•

To request a copy, visit www.neb.com

This year’s catalog features minireviews focused

on renewable energy generated from natural resources.

CLONING & MAPPINGDNA AMPLIFICATION

& PCRRNA ANALYSIS

PROTEIN EXPRESSION

& ANALYSIS

GENE EXPRESSION

& CELLULAR ANALYSIS

This annual international research prize recognizes

accomplishments in neurobiology research based on

methods of molecular and cell biology. The winner and

finalists are selected by a committee of independent

scientists, chaired by the Editor-in-Chief of Science.

Past winners include postdoctoral scholars and

assistant professors.

To be eligible, you must be 35 years of age or younger.

If you´re selected as this year´s winner, you will receive

$25,000, have your work published in the prestigious

journal Science and be invited to visit Eppendorf in

Hamburg, Germany.

Deadline for entries

June 15, 2009

2008 Winner

Mauro Costa-Mattioli, Ph.D.

Department of NeuroscienceBaylor College of Medicine,

Houston, Texas

Eppendorf & Science Prize for

Neurobiology

For more information

www.eppendorf.com/prize

Get recognized!

$25,000Prize

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www.aacr.org/meetingcalendar

Conference Chairpersons:Arnold J. Levine

Institute for Advanced Study,

Princeton, NJ

Elizabeth H. Blackburn

University of California,

San Francisco, CA

Joan S. BruggeHarvard Medical School,

Boston, MA

Robert A. Weinberg

Whitehead Institute for

Biomedical Research,

Cambridge, MA

Frontiers in Basic Cancer Research will be a broad-based

meeting with the goals of presenting the best in basic

cancer research and giving early-career investigators the

opportunity to interact with leaders in the field.

Abstract Submission, Award Application, and

Early Registration Deadline: August 10, 2009

October 8-11, 2009 • Boston Park Plaza • Boston, MA

DREXEL UNIVERSITYcongratulates the recipient of its inaugural

ANTHONY J. DREXEL $100,000 EXCEPTIONAL ACHIEVEMENT AWARD

FOR EXCELLENCE IN TRANSLATIONAL RESEARCH

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University Professor and Professor of Biomedical Engineering, Boston University

Co-Director and Co-Founder, Center for BioDynamics, Boston University

Investigator, Howard Hughes Medical Institute

drexel.edu

JAMES J. COLLINS, PH.D.

www.sciencemag.org/products SCIENCE VOL 324 5 JUNE 2009 1341

AAAS/Science Business Office

New Products Focus: Cell/Tissue Culture

LIFE SCIENCE TECHNOLOGIESNew Products Focus: Imaging and Microscopy

CrossBeamWorkstationThe Auriga CrossBeam focused ion beam/scanning electron mi-

croscope workstation features a new chamber design and unique

charge compensation system for advanced analysis. The instru-

ment’s redesigned vacuum chamber includes 15 ports for different

detectors. Its charge compensation system enables the local ap-

plication of an inert gas flush. In this way, electrostatic charging of

nonconductive samples is neutralized and detection of secondary

electrons as well as backscattered electrons becomes feasible. The

heart of the workstation is the field emission-scanning electron mi-

croscopy column, which enables the analysis of magnetic samples.

Carl Zeiss

For information +49-73-64-20-21-94

www.zeiss.de

Fluorescent Blot KitSpectraPlex provides the tools to perform quantitative, two-color

fluorescent protein immunoblots. Multicolor detection makes it pos-

sible to simultaneously quantify a loading control and a protein of

interest or phosphorylated isoforms of a single protein, which great-

ly increases the information from a single blot while saving time and

reagents. The kit includes fluorescently labeled secondary antibod-

ies aswell as blocking andwashing buffers optimized to provide high

sensitivity; as little as 1 pg of protein can be detected. The excitation

and emission spectra of the SpectraPlex secondary antibodies are

compatible with common fluorescence imaging systems.

Alpha Innotech

For information 800-795-5556

www.alphainnotech.com

Scanning Probe MicroscopeThe Hydra is designed to open new horizons for the application of

Cell Imaging SystemThe IN Cell Analyzer 2000 is a flexible cell imaging system for high content

analysis with high image quality, speed, and ease of use. The system can per-

form a wide variety of challenging experiments, from investigative micros-

copy through automated screening and imaging of organelles, cells, tissues,

and whole organisms. New features include rapid preview scanning of a se-

lected area of a sample at any available magnification prior to starting an ac-

quisition run; a high-performance large-chip charge-coupled device camera

combined with a widefield illumination source that is twice as bright as a con-

ventional xenon lamp, ensuring statistically robust results in a single pass;

and whole-well imaging to capture an entire well in a single image, enhancing

the ability to image rare events. A wide range of objectives are available to

suit a variety of sensitivity requirements.

GE Healthcare

For information +44-(0)20-7866-7862

www.gehealthcare.com/incell

atomic force microscopy (AFM) in biology. The ground-up design ofthe Hydra provides no geometric or optical obstruction or interfer-ence either from above or below the scanning probe microscopesystem. It incorporates a tuning fork feedback mechanism to pro-vide new sensitivities. The Hydra comes with a NanoToolKit of glass-based probes, including transparent AFM probes and nanopipetteprobes for conductance and structurally correlated patch-clampapplications. The instrument is suitable for all modes of optical mi-croscopy, including standard fluorescence, confocal, total internalreflection fluorescence, fluorescence resonance energy transfer,and differential interference contrast techniques.Nanonics ImagingFor information 800-289-7162www.nanonics.co.il

Optical Metrology InstrumentThe LEXT OLS4000 is the latest version of the LEXT confocal laser

scanning microscope metrology system. It offers enhanced function-

ality, including near-vertical slope capabilities, large optical zoom,

and a navigation overview window. Its software brings complex

processes within easy reach of a broad range of users. The system

has a sleek look and requires only a single control unit. It has been

designed with a large and fast scanning mirror along with dual pin-

holes; the large mirror enables the system to provide superior opti-

cal quality, and the increased scan speed halves the time it takes

to create a three-dimensional image of a sample. The 405-nm laser

and dual pinholes operate simultaneously to ensure that the system

provides high resolution and clarity as well as enabling steep slope

detection up to 85°, so even the most complex surface topologies

can be imaged and analyzed.

Olympus Europe

For information +49-40-2-37-73-5426

www.microscopy.olympus.eu

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizationsare featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any prod-ucts or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.

High-end Quality

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Leica Microsystems

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Mr. Gunkel, why are DIN and ISO norms

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According to physicist Claus Gunkel, Head of the Leica Optic Centre, high-end quality starts in the

process – which begins by listening carefully. Users are included in a quality consciousness which

represents far higher demands than international standards: for top-performance optics produced

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