Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on...

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Gel electrophoresis L Mathias

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Transcript of Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on...

Page 1: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

Gel electrophoresis

L Mathias

Page 2: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

Definition

• Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied.

Page 3: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

DNA negatively charged

Page 4: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

DNA cut up using restriction endonucleases

Page 5: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.
Page 6: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

Make up the gel which the DNA will be put into

Page 7: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.
Page 8: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.
Page 9: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.
Page 10: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

Dye added to the DNA

Page 11: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

Buffer solution added to the tank

Page 12: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

DNA samples loaded into wells

Page 13: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

Electrical current applied to the chamber

Page 14: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

DNA is stained using ethidium bromide

Page 15: Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

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