GC/MS-Komplettlösungen von Agilent. Der schnellste Weg … · GC/MS-Komplettlösungen von Agilent....
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Transcript of GC/MS-Komplettlösungen von Agilent. Der schnellste Weg … · GC/MS-Komplettlösungen von Agilent....
GC/MS-Komplettlösungen von Agilent. Der schnellste Wegzur sicheren Methoden-Implementierung für Ihr Labor
GC MS Euro Tour 2011Page 1
Bernhard Rothweiler
Senior Applications Chemist
Europe, Middle East, Africaand India
New technologies essential to
meet higher productivity needs but…
• Requires time for startup
• Requires high investigation costs
• May generate questionable results
GC MS Euro Tour 2011Page 2
• May generate questionable results
Agilent Analyzer:
Get on a fast track to higher productivity
What is different when ordering an Analyzer?
CheckoutSystem with
#SP1: Factory setup and tested analyzer
Familiarization
Customer CD
Customer CD
w/o Analyzer
GC MS Euro Tour 2011Page 3
IndividualComponents
produced
IndividualComponentsTested (OFN)
Systemassembled
App. methoddeveloped oncust. system
Systemcheckout on
App. mix
Run 1st
customersample
customizationor optimization
method
On-goingmaintains and
service
Factory CE Setup; I&F Optional Consulting After InstallationWithout Ordering Analyzer
Factory Setup
Optional
Consulting After InstallationOrder Fast Track AnalyzerCE Specialist
I&F
reportapplication setup & consultingApp. optimized
consumablesStd. HW, SW
No More Struggle for DevelopingMethods
Sampleready
OrderStandardcheckout
Standardfamiliarization
GC MS Euro Tour 2011Page 4
Agilent developsmethod
Orderkit
Applicationcheckout
Applicationfamiliarization
RefinemethodOrder
• Universal methods to startup application , meeting most testing needs!
• Analyzers are flexible, can be adapted to customer needs
MSD Analyzer
Pesticide
GC/MSD Analyzer ( Backflush, MMI, DRS, Pesticide library)
GC/MSD Analyzer ( Backflush, MMI, DRS, Jap. List Pesticide library)
GC/MSD Analyzer ( Backflush, MMI, FPD, ECD, DRS, Pesticide library)
Forensics2 Toxicology Screening GC-MS Analyzer
GC MS Euro Tour 2011Page 5
2 Toxicology Screening GC-MS Analyzer
Backflush, MMI, SIM/Scan/NPD, DRS, Forensic/Tox library
Backflush, S/S inlet, SIM/Scan, DRS, Forensic/Tox library
VOC‘s
HSS/GC/MSD Analyzer ( S/S or MMI, SIM/Scan, DRS, Solvents+ library)
PAH‘s
GC/MSD Analyzer ( Backflush, MMI, DB-EUPAH column )
926 Pesticide Analyzer
Factory • All checkout on your system• System setup and instrument leak tested• Application appropriate column, liner and septa pre-installed• Retention time locked on your instrument• Capillary flow device setup for backflushing• DRS, AMDIS & NIST & RTL Pesticide libraries installed• Run pesticide checkout mix – perform DRS analysis• Signal/Noise check
Delivery • Quick start guide for checkout method
GC/MS Pesticide Analyzer
GC MS Euro Tour 2011Page 6
Deliverywith EasyTools
• Quick start guide for checkout method• All factory checkout results on CD ROM• Training videos• Consumables included, no separate ordering required• Trial SPE QuEChERS kits (AOAC and EN methods)• Easy consumables re-ordering information
ApplicationfocusedInstallation
• Agilent CE makes sure you are ready to runreal samples before he/she leaves
State-of-the-Art Technologies to Improve
Pesticides Screening with Agilent GC/MSDAnalyzer
Deconvolution
Reporting SW (DRS)
Retention Time
Locking (RTL)
Capillary Flow
Technology (CFT)
GC MS Euro Tour 2011Page 7
RTL databases
for 926 pesticide compounds
App. specific consumables
Sample Prep. QuEChERs
Multi-Mode Inlet,
SIM/SCAN; QEdit
Agilent Pesticide Analyzer:
Data File:
Adjacent Peak Subtraction = 1
Resolution = Medium
Sensitivity = Medium
MSD Deconvolution
Sample Name: carrot1 …to here in 2 - 3 min
from here…
Screen for 926
GC MS Euro Tour 2011Page 8
Chem R.T. Diff
station sec.
4.4196 33704619 Cashmeran 13.59 60 13.1 67 35
6.9401 1517222 Phenanthrene-d10 35.96 24.68 92 9.2 79 2
8.0245 84695 Diisobutyl phthalate 16.19 9.88 83 9.2 81 9
9.27 84742 Di-n-butylphthalate 61.5 23.38 95 7.5 92 1
12.136 72559 p,p'-DDE 6.15 3.52 87 15 82 1
12.9476 72548 p,p'-DDD 2.6 1.55 69 12.5 77 5
12.954 789026 o,p'-DDT 2.6
13.5916 50293 p,p'-DDT 2.85 1.33 70 12 71 3
13.9963 115866 Triphenyl phosphate 15.11 9.18 95 16.1 84 1
14.9048 117817 Bis(2-ethylhexyl)phthalate 4.46 56 9.6 64 62
14.99 2385855 Mirex 1.38
6.943 Phenanthrene-d10 10
Data File:
Date/Time: 2:45:59 PM Thursday, February 19, 2009
Sensitivity = Medium
Shape Requirements = Medium
Match Reverse
Match
Hit
Num.
The NIST library was searched for the components that
R.T. Cas # Compound Name AMDIS
Amount (ng) AMDIS NIST
Screen for 926
pesticides in
one 21-minute
GC/MS run
50
170 280
31075
185
160
How Does Deconvolution Work?
GC MS Euro Tour 2011Page 9
Extracted IonChromatograms
(EIC)
After de-skewing
50
170
280
75 late retention time185 shape & early retention time
310 early retention time
160 shape
same shape and sameretention time
AMDIS Deconvolution Pulls Out IndividualComponents and their Spectra
Deconvoluted components and spectra
matrixTIC
Component 1
Component 2
Component 3
Components and MixedSpectra
GC MS Euro Tour 2011Page 10
target
interferenceComponent 3
Deconvolution
DRS Report from 3 Integrated Processes
Total ion chromatogram
Targets are identified byDeconvoluted Target
AMDIS deconvolutescomponent spectra and
GC MS Euro Tour 2011Page 12
Combined quantitative and qualitative
HTML summary report
Quant Results
Targets are identified bycomparison to locked R.T.sand 3 qualifier ion ratios,then quantified using targetion area vs ISTD cal table
Deconvoluted Targetspectra confirmed byAMDIS, searched againstNIST08 MS database
Confirmed NIST08 hits
component spectra andsearches target MS databaseusing locked RT as a qualifier
Confirmed AMDIS hits
AMDIS Quant Result
Spinach Report, results from 3 DRS processes
NIST 08 Match and Hitnumber of the top 100hits from 191,000+compounds
GC MS Euro Tour 2011Page 13
ISTD, used for targets amount
Match against
AMDIS library
RT diff in sec. vsexpected locked RT
Configuration of New Tox AnalyzerPost column Capillary Flow Technology (PUU) device provides:
A) BackflushingB) No vent column changingC) Overpressure protection
LiquidInjector
AUX EPC
2.73 psig
VentPurged Ultimate
Union (PUU)
GC MS Euro Tour 2011Page 14
7890AGC
S/S Inlet
Column
15 m X 0.25 mm id X 0.25 um DB-5MSUI
5975CMSD
Diffusion
(or Turbo)
1m x 0.15 mm id
PUU
DRS Report From Blood Sample
AMDIS is used for compound identification. ChemStation is usedfor quantitative measurement of compound.
GC MS Euro Tour 2011Page 16
GC/MS/MS Analyzer
GC MS Euro Tour 2011Page 17
MS1 Quad andPost Filter
CollisionCell
Pre Filter and MS2Quad
DetectorSource
7000 B MS/MS SystemOptimized for gas chromatography
MS/MS Ensures Lowest Detection LimitsEI: spectrum of analyte can also includeions from matrix, column bleed, gases, etc.
Product 1
Product 2
GC MS Euro Tour 2011Page 18
Q1 SIMisolate precursorbefore CID
Product 1Product 3
CID +
Q2 SIM
Lower m/z ProductIons measured againstzero chemical noise
chemical noisefrom these otherions is eliminated
Apple
Cabbage
SIM - EIC (246) MRM (2 transitions)
S/N = 448
S/N = 241
Consistent, High Quality ResultsSIM versus MS/MS Comparison: Food Safety
GC MS Euro Tour 2011Page 20
Orange
Spinach
Ginseng
S/N = 456
S/N = 260
S/N = 446
State-of-the-Art Technologies to ImprovePesticides Analysis with Agilent
GC/MS/MS Analyzer
Dynamic MRMRetention Time
Locking (RTL)
Capillary Flow
Device for Backflushing
GC MS Euro Tour 2011Page 21
MRM DB for 500+Pesticides
Locking (RTL)
App. specificconsumables
Sample Prep.QuEChERS
Multi-Mode Inlet,
Cold Splitless
SP1 Pesticide Analyzer MRM Methods
7890-0501 7890-0502
setup post-column BF mid-column BF
flow control constant pressure constant flow
# of Analytes in DB 500+ 200+
# of Analytes in method 200+ 200+
GC MS Euro Tour 2011Page 22
# of Analytes in method 200+ 200+
RTL yes, to Agilent method no
Method/DB origin Agilent FDA and Agilent
inj mode(s) hot SL and cold pulsed SL cold pulsed splitless
Backflush post run post run
runtime 42 min 20 min
coolant N2 or Air (preferred) N2 or Air (preferred)
At User’s LabAt Factory
GC/MS/MS Pesticides AnalyzerDatabase for ~1000 pesticides under development
MRM DB for
Dynamic MRMRetention Time
Locking (RTL)
App. specific Multi-Mode
Capillary Flow
Device forBackflushing
Plug and Play
Ready forReady for
GC MS Euro Tour 2011Page 23
MRM DB for500+
Pesticides
App. specificconsumables
Sample Prep.QuEChERS
Multi-ModeInlet,
Cold Splitless
Ready forReady for
the 1the 1stst runrun
Triple Quadrupole GC/MS PAH AnalyzerA ready-to-use system for fast, sensitive PAH analysis
The following components are included – saving youtime and money:
• Retention Time Locked DB-EUPAH column (20 m x 0.18 mm x 0.14 μm) optimized and tested for PAH analysis
• PAH calibration standards and internal standards used for method
GC MS Euro Tour 2011Page 24
• PAH calibration standards and internal standards used for methodcheckout
• PAH MRM acquisition method, quick start guide, and Application Notethat show you how to set up the analysis method provided with theAnalyzer
• CD-ROM with factory checkout results, data files, and reports
Thank you !!!Thank you !!!
Questions???Questions???
GC MS Euro Tour 2011Page 25