Functional Magnetic Resonance Imaging - Lehigh …inbios21/PDF/Fall2007/Ferris_101007.pdf ·...
Transcript of Functional Magnetic Resonance Imaging - Lehigh …inbios21/PDF/Fall2007/Ferris_101007.pdf ·...
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Functional Magnetic Resonance Imaging
Bridging the Science Between Animal and Human Research
Craig Ferris, Ph.D. DirectorCenter for Comparative Neuroimaging
Department of PsychiatryUniversity of Massachusetts Medical School
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Center For Comparative Neuroimaging
mouse rat
marmoset rhesus
University Massachusetts Medical School
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Magnetic Resonance Spectrometer(4.7 T)
RF Probes
Computer Console
Passive Shielding
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Magnetic Resonance Imaging “Then & Now”
Damadian et al., Science 194:1431,’76 CCNI, 9.4T
Kramer et al., Neuroradiology 21:239 ’81 - Lauterbur
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High Resolution Magnetic Resonance Image Of Marmoset Monkey Brain
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Superconducting Magnets
Generate External Magnetic Field - B0
Field Strength Between 1.5 – 11.7 Tesla (T)Operate Near Absolute Zero Temperature
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Manipulating Hydrogen Atoms
MRI is non-invasive and uses the protons from mobil hydrogen in water and fat to generate signals
The hydrogen nucleus spins creating a local magnetic field causing the charged nucleus to act like little magnet aligning in BO.
Over time (2-3 sec) protons come to equilibrium or net magnetization MO
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Hydrogen Protons “Wobble”Not only does the nucleus of the hydrogen atom spin like a top but it also precesses or “wobbles.”The precessional frequency is defined by the Larmor Equation ω = γ BO
γ = 42.6 MHz/T for hydrogenWhen the field strength increases so does the precessional frequency
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Flipping Hydrogen Protons To Get MR Signal
An RF pulse with the same precessional frequency can “flip” the proton into the transverse plane
Protons precess around the transverse plane giving off an oscillating signal
Protons are initially in phase but rapdily dephase and signal decays in 20 to 30 msec.
The time constant that defines the rate of decay is T2
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•Spectroscopy
•Quantitative Anatomy
•Tract Tracing with Manganese Chloride
•Angiography
•Diffusion Weighted Tensor Imaging
•Diffusion/Perfusion Imaging
•Function ImagingCBF – cerebral blood flowCBV – cerebral blood volumeBOLD imaging
What can a neuroscientist learn about the brain using a magnet?
Ferris et al., J Neuroendocrinology 18:307, 2006
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Diffusion Weighted Tensor Imaging
Applications• brain myelination and
development• neurodegeneration
unpublished data, CCNI 9 4T
DT maps of marmoset
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Applications• phenotyping transgenic mice • developmental biology • neurotoxicology
MR Spectroscopy Quantifying Brain Chemistry
unpublished data, CCNI 4.7T
Brain EtOH Levels
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Tract Tracing with Manganese Chloride
unpublished data, CCNI 9.4T
Absence Status Seizure
Application• tracing functional
neural circuits
MnCl2injection site
Mn2+ accumulation 8 hr after injection
in-plane resolution 50 µm2T1-weighted contrast
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Basal condition
BLO
OD
FLO
W
Δ BOLD SIGNAL
4 Sec
HbFe2+O2
HbFe2+O2
HbFe2+
HbFe2+
O2
Initial increase in metabolism
Reactive hyperemia
Ogawa et al., MRM 14: 68-78 1990
O2
Functional Imaging Using BOLD BOLD Signal
Blood Oxygen Level Dependent
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BOLD Imaging and Psychiatry: A Critical Tool for Studying Brain Function
Since Ogawa’s original publication there have been close to 1,800 publications on BOLD imaging.Only around 300 have used animals – why?
They don’t sit still.
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Why Conscious Animals?
Tenney et al., Epilepsia 44:995, 2003
• BOLD Signal is depressed with anesthesia
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S1FL
S2FL
CPu
Awake Anesthetized (α-chloralose)
0 60 120 180 240-20
-15
-10
-5
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8.9 %
Time (x 1 second)
BO
LD p
erce
nt c
hang
es fr
om S
1FL
-5
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CPu
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CPu
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erce
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hang
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om S
1FL
Time (x 1 second)
BOLD Signal Changes In Awake vsAnesthetized Rats
Sicard et al., Cereb Blood Flow Metab 23:472, 2003
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Getting Started: Acclimating Animals to the Imaging Protocol
Physiology and Stress Hormone
King et al., J Neurosci Methods 148:154-60; 2005
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Atlas
Three Dimensional Segmented Rat Atlas
Registration “Group Statistics”
Segmentation
Activation Maps
3D Visualization
GUI
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Stimulation Paradigms“How do you talk to an animal in a magnet?”
• Odors
• Visual Images
• Tactile Stimulation
• Drug Challenges
• Complex Environmental Cues
“vivarium”
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Applications In Neuroscience
Imaging emotional states, e.g. sexual arousal, hunger, fear, etc.
Developmental studies on neurological disorders, e.g. stroke, seizure, dementia, etc.
Drugs and brain function, e.g. cocaine, MDMA, anxiolytics, neuroleptics, etc.
Learning and memory
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PRESENT AREAS OF RESEARCH
Maternal BehaviorStressDrugs of Abuse• MDMA “ecstasy” • Cocaine• Nicotine
AggressionFear and AnxietyDrug Discovery
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MOTHER/INFANT BONDING
Early maternal care is critical for the psychosocial development of infants.
With the evolution of mammals, nursing has helped strengthen the mother/infant bond and extend the period of parental investment in young.Courtesy of Steve Suomi
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a
b c
Volume coil
Body tube opening exposes the ventrum
Weigh boat cradle padded with gauze 4 pups inside cradle
under the ventrum
Can We Image the Nursing Brain?
Yes …
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Interestingly, the Brain Activity Suggests Suckling is a Rewarding Experience
• Mesocorticolimbic dopaminergic pathways are activated.
• Previous studies show suckling has many of the properties of addictive drugs. Suckling motivates bar pressing for pups and conditioned place preference.
(Fleming, Spear, and Morrell labs)
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Is motherhood more rewarding than cocaine?
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… it would seem so.
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MARMOSET FAMILIES
“MOM, DAD, & THE KIDS”
MONOGAMOUS
TWINS
AFFILIATION
DEVELOPMENT
SMALL SIZE
Courtesy of Nancy Shultz-Darken
Primatologists and collaborators:
•Charles Snowdon
•Toni Zeigler
•Nancy Shultz-Darken
•Melinda Novak
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0
100
200
300
400
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1 2 3 4midday baseline*
habituation baseline
habituation post-restraint
unstable peer group*
*Wisconsin Marmoset Colony
plas
ma
cort
isol
(µg/
dl)
Stress-Induced Changes in Plasma Cortisol
simulated imaging session
Habituating Marmosets to the Stress of Restraint and Imaging
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Choice of Anesthetic
• sodium pentobarbital
• chloral hydrate
• propofol
• isoflurane
• medetomidine/atipamezole
(Domitor/Antiseden)
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Marmoset imaging restrainer and RF
electronics
Brain Activity and Recreational MDMA “Ecstasy”
To date, no one has done fMRI studies on humans or monkeys to observe changes in brain activity following oral MDMA.
post imaging
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Brain Activation Following Oral MDMA (1mg/kg)
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Activation of Limbic Circuitry Following Oral MDMA
N=6
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05
1015202530354045
Veh+1 3.3+10
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Adolescence vs Adulthood
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PPI%
Male vs Femaleveh/1mg
veh/1mg
veh/1mg
veh/1mg
% p
repu
lse
inhi
biti
on%
pre
puls
e in
hibi
tion
3mg/10mg3mg/10mg
3mg/10mg 3mg/10mg
Adolescent females are most vulnerable to the MDMA
Pre-MDMA
Post MDMA
*
*
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A Special Thanks to…
Tara Messenger
Praveen Kulkarni
Marcelo Febo
Josie Harder
Mathew Brevard
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SUPPORT
National Institute of Mental HealthNational Institute on Drug Abuse
&Insight Neuroimaging Systems (INSL)* Booth #1610
&Azevan Pharmaceuticals**
*Disclosure: Craig Ferris has a financial interest in INSL, the bioengineering company that produces the RF electronics and holders for animal imaging.
**Disclosure: Craig Ferris has a financial interest in Azevan, the pharmaceutical company developing drugs for the treatment of psychiatric disorders.
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Animal Imaging Has Its Limits
Thank You