Fullerene, Super Anti-Oxidant

-Clinical Test Results on Whitening and

Anti-Aging-

August 30th , 2013

Vitamin C60 Bioresearch Corporation Tomoko OHTSU

2

Minimum Knowledge about Fullerene!

1. Fullerene(C60) is a carbon allotrope of carbon, like a diamond.

2. Fullerene(C60) is a Nobel Prize winning material

3. Fullerene(C60) is a super anti-oxidant.

- 172 times as effective as Vitamin C

- stable against light and/or heat

- power is long-lasting (more than 24 hours)

Great Effects of Fullerene

Anti-aging（preventive effects of cell death)

Inflammation Easing

(reducing of a ruddy face)

Anti-Wrinkle

Sunscreen / UV cut Whitening

（Melanin Control）

Sebum Oxidation Control

Pore Tightening

3

Recover

Skin barrier

1. Free Radical Scavenging activity

2. Comparison data with

other anti-oxidant

ingredients

3. Preventive Effect of Fullerene

4

Data of Fullerene Effect

- in vitro Study-

Free Radical Scavenging activity

high

low

Co

ncen

trati

on

of

Fre

e

Rad

ical

Without Fullerene With Fullerene

5

-20

0

20

40

60

80

100

Linoleic acid H2O2 UV rayAn

tio

xid

an

t A

cti

vit

y (

AO

A%

)

Vitamin E Vitamin C Derivative(APS) Fullerene(Radical Sponge)

Radical scavenging activity of Fullerene

6

PV

P/C

60

PV

P/C

60O

PV

P/C

70

PV

P/C

60O

n

cate

chin

vitam

in E

isoflavone

coenzym

e Q

10

querc

etin

cru

cm

in

AP

PS

a-lip

oic

acid

An

tio

xid

an

t

Acti

vit

y(A

OA

%)

0

20

40

60

49.5

35.6

29.1

25.1

17.7 14.8

7.35

An

tio

xid

an

t A

cti

vit

y(A

OA

%)

Comparative testing with other antioxidant

ingredients by β-Catotene method

7

Preventive effect of Fullerene

11 h

ours

late

r

Without Fullerene With

Fullerene

8

9

Clinical Test Results by using Fullerene

contained cosmetics

1. Brightening (Whitening) Effects

(Radical Sponge™ )

2. Effect of Fullerene on the Skin Barrier

(Radical Sponge™)

3. Fullerene is effective against wrinkle

( LipoFullerene™ )

Brightening (Whitening) Effects

by Fullerene

Effectively eliminate UV-induced free

radicals

Prevent excessive melanin production

Verified by various clinical trials

10

Suppression of Melanin production by Fullerene

Without Fullerene With Fullerene

Let’s check the movie!!

11

Me

lan

in In

de

x

0

0.5

1

1.5

0 4 8 （weeks）

He

mo

glo

bin

In

de

x

p < 0.001

p < 0.001

0 4 8 (weeks）

1.3

1.2

1.1

1.0

0.9

0.8

0.7

0.6

p < 0.001

Brightening (Whitening) Effect by Fullerene

Subjects: 18 females aged 34 to 49 Area: Face

The melanin index decreased intentionally on the 4 weeks.

13

You can feel the Whitening/Brightening effect in about 1-2 month.

Continuous use of Fullerene contained product is the key to have better

results.

After 5 month

After 3 month

Continuous use of Fullerene contained product will lead you

more brighter and fair skin condition.

Brightening (Whitening) Effect by Fullerene

14

Effect of Fullerene on the Skin Barrier

by Fullerene

Increase “Transglutaminase 1” mRNA

expression.

Increase “Cornified Emvelope” formation and

help maintain Skin barrier function.

Clinical study: Help in recovering skin barrier

function.

15

Structure and function of the stratum corneum

Intercellular lipid

Stra

tum

co

rne

um

20 μ

m

Gra

nu

lar la

ye

r Normal stratum corneum

Cornified

cell

Cornified

envelope

（CE）

Moisture loss

Stratum corneum with decreased

skin barrier function

flecks

wrinkles

dullnes

s

rough

skin

dry

skin

・drying

・UV

・stress

・aging

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Clinical Test :

Tape Stripping Method to measure Trans Epidermal Water

Loss (TEWL) Radical Sponge® helped recovery of TEWL on 2nd and 3rd day

significantly

-40

-20

0

20

40

60

80

100

0 1 2 3 4

Rate

of

reco

very

（％）

#P<0.05 vs. RS(0%) # #

RS(0%

)

RS(1%)

days

0 1 2 3

4 Trial Design Randomized blinded study

Method TEWL changes after Tape Stripping

Subjects 10 healthy subjects (5male 5female)

Trail period and applications Solution was applied twice daily for 5days

17

Fullerene is effective against wrinkle

by Fullerene

Remove the Free Radicals which is the

primary cause of wrinkles.

Skin structure is improved steadily.

Fullerene/squalene cream significantly

reduced wrinkle area compared to the

placebo-treated area.

18

Wrinkles formation by ultraviolet rays

Decrease of MMP9 and FOS mRNA means repression of wrinkles formation.

RO

S

UVB Wrinkle

formation

FOS Epidermi

s

Basement

membrane

Dermis

laminin-5

IV type collagen

VII type collagen

The active oxygen which occurred by ultraviolet rays activates FOS

(intracellular signaling) and MMP9 (degradative enzyme of collagen),

and forms wrinkles.

MMP9（Matrix metallopeptidase 9）・・・ degradative enzyme of laminin-5 etc. 19

The percentage of wrinkle area decreases in the

LipoFullerene application group than a Placebo application

group.

Fullerene is effective against wrinkle

by LipoFullerene

20

Guideline

s

Guidelines for Evaluation of Anti-

Wrinkle Products prepared by the

Japanese Cosmetics Science Society

in 2006

Trial

Design A randomized, double-blind test

Trial

Period

Eight weeks (September 24, 2008 ～

November 19th, 2008）

Subjects 23 Japanese women in their 30s to

40s with wrinkle grade 2 to 3.

Test

Samples

1%-LipoFullerene-blended cream

(active) and Lipofullerene free cream

(placebo)

10 x 10 mm

Skin surface replica Shadow image

Wrinkle: few many

Evaluation

Great Effects of Fullerene

Anti-aging（preventive effects of cell death)

Inflammation Easing

(reducing of a ruddy face)

Anti-Wrinkle

Sunscreen / UV cut Whitening

（Melanin Control）

Sebum Oxidation Control

Pores Tighten

21

Moisturizer

(Skin barrier)

22

For Basic Skin Care Products and MORE!

Basic Skin Care Products - Lotion

- Essence/Serum

- Cream

+

1. Color Cosmetics

(BB Cream, CC Cream)

2. Hair care

(Scalp tonic,

Shampoo & Treatment)

3. Beauty Laser care

4. Sun screen

5. Facial Mask

Fullerene Products in

Overseas

Anti Anti - - oxidant Whitening Serum oxidant Whitening Serum

With Fullerene RS With Fullerene RS ™ ™

23

0

200

400

600

800

1000

1200

1400

1600

2005 2007 2009 2011 2013

Number of Clinics

Fullerene is famous for Doctor’s Cosmetics

More than 1,500 clinics in Japan has selected our Fullerene cosmetic

ingredients!!

24

2005 Launch Radial Sponge

2007 Entered to Internet Market

2006 Start Selling in Overseas（Korea・Taiwan・USA）

2008 1000 Clinics selected Fullerene

2009 Launch LipoFullerene

2011

For Clinical Doctors

Dr.Brandt （USA）

Dr.Ci labo

DHC

Over 1000 items are selling in the market !!

Felice Towako Cosmetics

NOVA C60（Canada）

MISSHA

（Korea）

マードゥレクス

CBON

アジュバンコスメ

Dr.Perricone（USA）

JMEC

Derfarma

AR

Doctor Products

WithUs

アプロ サイエンス

TUNEMAKERS

Dr.WU（Taiwan）

Dr. Recella

Unicity

St.Clare （Taiwan）

ISA Nox (Korea）

Water-Soluble Fullerene

Oil-Soluble Fullerene

Fullerene Contained Products

25

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Radical Sponge

(water-soluble)

LipoFullerene

(oil-soluble)

Our Products

Trademark (LOGO)

Products which containing more than 1% of our ingredients(Radical Sponge

or LipoFullerene) can put our trademark to their product package and web page. 27

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Marketing Points of Fullerene!!

Nobel Prized Winning Ingredient 172 times as effective as Vitamin C Stable against light(UV) and/or heat Power is long-lasting (more than 24 hours) Abundance of Clinical data Multifunctional ingredient Safety and secure ingredient selected by Clinic Doctors

Samples of our ingredient is available!!

After concluding Non-Disclosure Agreement (Sample

Evaluation Agreement), VC60 will send sample to the

customer for free.

• Radical Sponge (water-soluble Fullerene) : 20 g

• LipoFullerene (oil-soluble Fullerene): 16 g

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Sample formulations are available!!

• Radical Sponge (water-soluble Fullerene)

Serum & Cream

• LipoFullerene (oil-soluble Fullerene):

16 g

Milk Lotion W/O type & O/W type

30

Exhibitions

in-cosmetics Asia 2013

Date: October 29th – 31st, 2013

Time: 10:00 – 17:00

Place: BITEC Hall Bangkok,

Thailand

Booth number: E10

(Booth share with Bronson &

Jacobs)

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Vitamin C60 BioResarch Corporation

Contact:

info@vc60.com

+81-3-3517-3252

http://www.vc60.com

Thank you for your attention!