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REMEDIAL INVESTIGATION REPORT FOR THE STANDARD CHLORINE OF DELAWARE, INC SITE DELAWARE CITY, DELAWARE APPENDICES G - L VOLUME m September 1992 Prepared by: Roy F. Western, Inc. 1 Weston Way West Chester,Pennsylvania 19380-1499 ftR3067i*8
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REMEDIAL INVESTIGATION REPORTFOR THE
STANDARD CHLORINE OF DELAWARE, INC SITEDELAWARE CITY, DELAWARE
APPENDICESG - L
VOLUME m
September 1992
Prepared by:
Roy F. Western, Inc.1 Weston Way
West Chester, Pennsylvania 19380-1499
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APPENDIX G
EXPOSURE DOSES
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APPENDIX H - ECOLOGICAL CHARACTERIZATION OFTHE WHITE-TAIL DEER
flR306778
STORY OF TEE WHITE-TAILED DEER
Range: Across s. Canada to c. British Columbia and throughout the United States,except for most of California, Nevada, Utah, and w. Colorado. Range extends intoSouth America.
Relative Abundance in Delaware: Common.
Habitat: Forested edges, swamp borders, areas interspersed with fields and woodlandopenings. During winter months when snow depth exceeds 16 inches (40.6 cm), deerwill "yard" in stands of conifers, forming a central resting area with trails packedthrough the snow.
Special Habitat Requirements: Dense cover for winter shelter, adequate browse.
Reproduction: Age at sexual maturity: some females mate as yearlings, most malesand females are mature at 18 months. Breeding period: late October to mid-December.Peak: November. Gestation period: 201 days. Young born: May and June with anextreme spread from March to September. Litter size: 1 to 4 fawns, average 2.
Home Range: 2 to 3 square miles (5.2 to 7.8 km2). Size depends on the quality of thehabitat. Home range is from 40 acres (16.2 ha) in excellent habitat to 300 acres (121.5ha) in poor habitat (Banfield 1974:392).
Food Habits: Mainly crepuscular. Deer browse on a variety of woody deciduous plantsand some coniferous growth, feeding on twigs and stripping young bark. Also graze ongrasses, herbs and mushrooms, and grub for roots. Adaptable in its foot habits.
Comments: Gregarious, usually forming in small groups. Family groups consisting of
STAND-CLVRI-SCT6A.RPT H-l MnOAC*7"7O
a doe with her fawns and yearlings are (sometimes) conmidn in the late fall.
Key References: Banfield 1974, Godin 1977, Taylor 1956.
STAND-CL\RI-SCT6A.RPT H-2
J*^ flR306780
APPENDIX I - ECOLOGICAL CHARATERIZATION OF THEGREAT BLUE HERON
AR30678
LIFE HISTORY OF THE GREAT TCTJTTR HF.KON
Range: Breeding: Southern Canadian provinces, s. to s. Mexico. Winter:Massachusetts s. through coastal states w. across the s. half of the United States, s. ton. South America. .
Relative Abundance in Delaware: Locally common (Maine) to rare (Massachusetts)in breeding season.
Habitat: Breeding: shallow shores of ponds, lakes, streams, rivers, wet meadows,wooded swamps, fresh bays, and marshes. Wintering: mainly coastal areas with bare(snow-free) ground and open water.
Special Habitat Requirements: Generally require tall tree for nesting.
Nesting: Egg dates: April 15 to June 9, New York (Bull 1974:73). Clutch size: 3 to7, typically 4. Incubation period: About 28 days (Bent 1926:106). Nestling period:About 60 days (Pratt 1970). Broods per year: 1. Age at sexual maturity: 2 years (Bent1926:108). Nest height: to 130 feet (39.6 m), typically high in large trees. Nest site:varies with habitat. Usually in tall trees but may be built on ground, rock ledges, andsea cliffs, or in shrub. Birds are typically colonial nesters but may be solitary. Samenest often repaired for use each season. Nest may be miles from food sources.
Territory Size: Sizes vary with habitat and stage of reproductive cycle. Colonialnesters defend small areas often restricted to the distance the bird can extend its neck andbill from the nest.
STAND-CL\M-SCT6A.:RPT
flR3067.82
Sample Densities: Dozens of nests may be built in crown of a single tree. 131 activenests per 0.36 ha (0.9 acre) in Oregon (Werschukel et al. 1976).
Foraging: Major foods: aquatic and terrestrial insects, fishes, amphibians, reptiles,crustaceans, occasionally small birds and mammals. Substrates: shallow water, muddybottoms, grasses and weeds. Techniques: stand and wait (Kushlan 1976, Willard 1977),walk slowly. Active pursuit is infrequent. Occasionally floats (Pough 1951:40).Preferred feeding habitat: wet meadows, pastures.
Comments: Individual feeding territories may be strongly defended during thenonbreeding season. Birds occasionally nest miles from food source. Commonly seenfeeding on tidal flats during migration.
Key References: Bent 1926, Cottrille and Cottrille 1958, Palmer 1962, Pratt 1970.
flR306783
APPENDIX J - SEDIMENT TOXICITY TEST RESULTS
flR30678l*
RQUfl SURV6V, INC
Summary
A sample of sediment (SSC-20-B) from the Standard Chlorineof Delaware site in Delaware City, Delaware were collected byWeston personnel and shipped to Aqua Survey, Inc. (ASI) onOctober 29, 1991. The sediment sample was tested to define theacute and chronic toxicity to the amphipod (Hyallela azteca).
The test sediment was mixed with a clean control sediment ofsimilar physical composition to create a series ofconcentrations. Juvenile amphipods were exposed to the sedimentfor tan days. Mortality was observed and a 10 day LC50 wascalculated to be 81.99%, using the Probit Method (see Table I).A random sample of the survivors were examined for length andAnalysis of Variance and Dunnett's Multiple Range Test were usedto determine a No Observed Effects Concentration (NOEC) of 12.5%and a Lowest Observed Effects Concentration (LOEC) 25.0% (seeTables II).
Grain size and moisture determinations were run on both thet@st sample and the control.
Page 1 of 9
499 Poir.t Breeze Roec • Rerrunsto", Kew Jersey G8-S22 « T&eD one .'9OS)7S5-S7O<
flOUR SURVeV, INC
Report: The toxicity of a sediment sample fromStandard Chlorine of Delaware, DelawareCity, Delaware.
Sponsor: Standard ChlorineGovernor Lea Road, PO Box 319Delaware City, DE 19706
TestingFacility: Aqua Survey, Inc.
499 Point Breeze RoadFlemington, NJ 08822
StudyNumber: 91-322
Report: 1748
SampleIdentification: SSC-20-B
Date StudyStarted: November 1, 1991
DateStudy Finish: November 12, 1991
DateReported: November 20, 19911
Personnel: C. Nally Y. TerrellS. Douglas S. GrassoR. Fristrom C* LawrenceE * Schwetje J. Banko
W. Scott Douglas DateStudy Director
Page 2 of 9
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flQUfl SURVeV, INC
I* Objective
The objective of this test was to define the acute andchronic toxicity of test sediment (SSC-20-B) from theSTANDARD CHLORINE OF DELAWARE site when in the environmentof benthic invertebrates. The measures of potency aresignificant reduction in survival or growth of exposedorganisms as compared to a control after ten days.
II. Test material
Source: Standard ChlorineGovernor Lea RoadPO Box 319Delaware City, DE 19706
Name: SSC-2-B
DateReceived: October 30, 1991
III. Materials and Methods
A. Method
The method employed was a modification of the methodoutlined by American Society of Testing Materials,Standard Guide for Conducting Sediment Toxicity testswith Freshwater Invertebrates, E1383-90.
B. Test Organisms
Species
The test species for this test was Hvallela azteca,which is a representative benthic invertebrate.
Size/Age/Phvsical Condition
Animals used in this test were amphipods, <14 days oldand appeared to be in good condition.
Page 3 of 9
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survey we.
Source/Acclimation
The animals used were cultured at Aqua Survey, Inc.
C. Test System
Source of Dilution Water
Dilution water was Round Valley Reservoir water(Lebanon, NJ) which was altered using standard EPAmethods to increase the hardness.
Temperature _= - ,-.... ...
The test temperature was 20+/- 2°C.
Test Vessels .. " .
The test vessels were 1-liter borosilicate glassbeakers covered with a petri plate and gently aeratedthrough glass tipped aeration lines.
Photoperiod
The test was conducted on a 16-hour light/8-hour darkphotoperiod.
D. Test Design
Test Levels
Three replicates of varying concentrations (6.25, 12.5,25.0, 50.0 and 100 percent by volume) of sediment weretested and compared to three replicates of controlsediment.
Page 4 of 9
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Control
A control sediment was obtained from Spruce RunReservoir in Annandale, New Jersey. The controls wererun using 100% control sediment and the same number oforganisms as the test concentrations.
Beginning the Test
The test concentration were prepared by mixing varyingvolumes of test sediment and control sediment to give afinal total volume of 600 cm3 for each concentration.The test was initiated by placing approximately 200 cm3of each test concentration into each of three replicatetest vessels and filling the vessels with dilutionwater. The system was allowed to settle under gentleaeration overnight. After settling, twenty animalswere chosen at random and gently added to the testvessels.
The test was of 10 days duration.
All test vessels were examined for survival and growthat 10 days.
Water Quality Measurements
Dissolved oxygen, pH, temperature and conductivity weredetermined at the start and end of the test in everytest vessel. Daily measurements of these parameterswere taken in one test vessel from each treatment.Total alkalinity and total hardness were measured ineach test vessel from each treatment at the start andend of the test.
Feeding
Animals were fed three times weekly on a diet ofcrushed Tetra Min and cereal flakes as required by ASTMguidance*
Grain Size
Particle size distribution was determined usingStandard Methods for Particle-Size Analysis of SoilsASTM Designation: D 422 - 63 (Reapproved 1972)American National Standard A37 145-1972 Approved March2, 1972 by American National Standard Institute.
Page 5 of 9
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Reference Toxicant
A standard reference toxicant test with Sodium DodecylSulfate was performed. The toxicant was dissolved indeionized water and mixed with culture water to achievethe desired concentration. No substrate was provided.
IV* Results
Average survival ranged from 25 to 95% in all replicates(see Table 1). An LC50 was determined to be 81.99%calculated using the Probit method. Mean length varied from1.33 mm to 1.93 mm* Analysis of variance testing withDunnett's procedure indicated significant reduction inlength of the surviving amphipods in concentrations 25, 50and 100%, as compared to the control.
The test solution temperature was maintained at 20 •*-/- 2°C(see Table III).
The pH ranged from 7.1 to 8*3 (see Table III).
Conductivity ranged from 315 to 480 umhos/cm. Dissolvedoxygen was kept at or above 5.5 mg/L in all test vessels.Alkalinity ranged from 8 to 108 mg/L. Hardness ranged from128 to 244 mg/L (see Table III).
Particle size distribution for the control and test sedimentwere similar enough as to eliminate physical toxicity (seeTable IV).
The 48 hour LC50 for the reference toxicant was determinedto be 10.08 mg/L.
V. Source of Documentation
All original data documentation is being maintained at:
Aqua Survey, Inc.499 Point Breeze RoadFlemington, NJ 08822
.VIPage 6 of 9
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TABLE I
Sample
CONTROL
6.25%
12.5%
25.0%
50.0%
100.0%
Percent Survival
A
85
75
90
65
80
35
of Amphipods
ReplicateB
95
90
90
90
70
40
after 10 Day Exposure
C Average-
90 90.0
85 83.3
90 90.0
80 78. 3 f
55 68.3*
25 33.3*-
TABLE II Hyallela Growth Summary
Sample
CONTROL
6.25%
12.5%
25.0%
50.0%
100%
Mean Lenath
1.93
1.70
1.64
1.46 *
1.43 *
1.33 *
S
0.24
0.04
0.07
0.14
0.22
0.01
* denotes statistically significant response at £0.05,
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•TABLE III Physical
Sample DissolvedOxygen (mg/L)
CONTROL 6.1-8.8
6.25% 6.5-8.8
12.5% 6.3-8.9
25.0% 7.0-8.9
50.0% 6.0-8.9
100% 5.5-8.9
Chemical Readings for Amphipod Test
pH Temperature
7.1-8.3 19.0-20.0
7.2-8.2 19.0-20.0
7.2-8.1 19.0-20.0
7.3-8.1 19.0-20.0
7.4-8.1 19.0-20.0
7.4-8.1 19.0-19.5
Conductivity(umhos/cm)
350-480
340-440
340-450
335-410
320-410
315-340
•
CONTROL
6.25%
12.5%
25.0%
50.0%
1 100%
Total "Alkal inity ( ma/Li
76-108
72-92
68-80
60-76
44-56
8-56
^^B Page 8 of 9
imfm 499 Point Breeze Roccf * Fterangior.. New jersey C3S22-* Teiep crs, (9
TotalHardness (mg/L)
136-208
180-232
156-244
128-196
128-188
128-172
OS}7SS- 37OG F.AB3s&6-7
SURV6V, INC.
A. Physical Testing
Grain size and water content determinations were made forthe test sediment and the control sediment.
Grain size distribution is defined by the followingcriteria:
Sand > or = to 0.0625 mmSilt <0.0625 but >0.0039 mmClay <0.0039 mm
See Table 4 for Grain Size and Percent Moistureresults.
Table IV Results of Grain Size and MoistureContent Analysis
Sample
Control
SSC-20-B
%Sand
13.0
14.00
%Silt
54.0
14.0
%Clay
33.0
30.0
%Moisture
57.73
29.76
Page 9 of 9
Cl/1 499 Point Breeze Road • w«ring*ar.. New Je-sey O3822 « Taepriorse (9O£}7SS-37OC d"? 0 -5 93
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EPA PROBIT ANALYSIS PROGRAMUSED FOR CALCULATING EC VALUES
Version 1.4
idard Chlorine H. azteca 10 day LC50Observed Ad j ust ed Predicted
Number Number Proportion Proportion ProportionCone . Exposed Resp . Responding Responding RespondingControl 60 6 0.1000 0.0000 0.12666.2500 60 10 0.1667 0.0458 0.000312.5000 60 6 0.1000 -.0305 0.006525.0000 60 13 0.2167 0.1031 0.058550.0000 60 19 0.3167 0.2176 0.2569DO. 0000 60 40 0.6667 0.6183 0.6034
- Square Heterogeneity » 2.3811.913740
"ina » 0.328974
Estimate Std. Err. 95% Confidence Limits
ircept -0.817289 1.410320 ( -3.581517, 1.946939)De 3.039749 0.763101 ( 1.544071, 4.535428)
0.126630 0*025999 ( 0.075672, 0.177589)ponse Rate
A-l
flR306795
dard Chlorine H. azteca 10 day LC50Estimated EC Values and Confidence Limits _
Lower UpperCone. 95% Confidence Limits
00 14.0749 2.7121 25.124100 23.5836 7.4013 35.9441
L .00 31*0546 12.5741 ,43.7373L5.00 37.3930 17.9007 50.1558
00 81.9860 65.2608 109.210500 179.7582 127.7165 442.990800 216.4479 145.8972 633.0807
55.00 285.0163 176.9761 1078.8999.00 477.5656 252.5387 2951.9468
A-2
flR306796
andard Chlorin© H. azt©ca 10 day LC50PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
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andard chlorine definitive test, growth data*: stc!2 Transform: NO TRANSFORMATION
Wilks test for normality
0.262
0.958
Laical W (P = 0.05) Cn = 18) = 0,897itical W (P « 0.01) (n = 18) - 0.858
La PASS normality test at P=0.01 level. Continue analysis,
andard chlorine definitive test, growth dataLe: stc!2 Transform: NO TRANSFORMATION
.:letts test for homogeneity of variance
nilated B statistic =14.50Lie Chi-square value -= 15.09 (alpha « 0.01)ble Chi-square value = 11-07 (alpha =0.05)
rage df used in calculation ==> df (avg n - 1) = 2.00e±d for Chi-square table value ==> df (#groups-l) = 5
S homogeneity test at 0.01 level. Continue analysis
If groups have unequal replicate sizes the average replicate size isused to calculate the B statistic (see above).
E: standard chlorine definitive test, growth data5: stc!2
kNSFORM: NO TRANSFORMATION NUMBER OF GROUPS: 6
IDENTIFICATION REP VALUE ' TRANS VALUE
control 1 1.9400 1.9400control 2 2.1600 2.1600control 3 1.6800 1.6800
6.25 1 1.6600 1.66006.25 2 1.7400 1.74006.25 3 1.7000 _ 1.700012.5 1 1.6100 1.610012.5 2 1.7200 1.720012.5 3 1.5900 1.590025 1 1.3100 1.310025 2 1.5800 1.580025 3 1.5000 1.500050 1 1.6800 1.680050 2 1.3200 l."320050 3 1.2875 1.2875
X 1.3333 1.3333 «nor^->,-v~A-4 flR306798
100 2 1.3250 1.3250100 3 1.3200 1.3200
andard chlorine definitive test, growth datai t: stc!2 Transform: NO TRANSFORMATION
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 1 Of 2
IDENTIFICATION N MIN MAX MEAN
Control 3 1.680 2.160 1.9276.25 3 1.660 1.740 1.70012.5 3 1.590 1.720 1.64025 3 1.310 1.580 1.46350 3 1.288 1.680 1.429100 3 1.320 1.333 1.326
indard chlorine definitive test, growth data: stcl2 Transform: NO TRANSFORMATION
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 2 Of 2
IDENTIFICATION VARIANCE SD SEM
control 0.058 0.240 0.1396.25 0.002 0.040 0.02312.5 0.005 0.070 0.04025 0.019 0.139 0.08050 0.047 0.218 0.126100 0.000 0.007 0.004
chlorine definitive test, growth dataLe: stcl2 Transform: NO TRANSFORMATION
ANOVA TABLE
*CE . DF SS MS F
een 5 0 . 717 0 . 143 6 . 500
iin (Error) 12 0.262 0.022tal 17 0.979
critical F value = 3.11 (0.05,5,12)Since F > Critical F REJECT Ho:All groups equal
*ndard chlorin© definitive test, growth data: stc!2 Transform: NO TRANSFORMATIONDUNNETTS TEST - TABLE 1 OF 2 Ho:Control<Treatment. - ___
MEAN CALCULATED INLOUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT SIG
control 1.927 1.9276.25 1.700 ,. 1.700 1.87212.5 1.640 1.640 2.36725 1.463 1.463 3.826 *50 1.429 1.429 4.108 *100 1.326 1.326 4.959 *
i: lett table value =2.50 (1 Tailed Value, P O.05, df=12,5)
idard chlorine definitive test, growth data 'xe: stc!2 Transform: NO TRANSFORMATION
DUNNETTS TEST - TABLE 2 OF 2 Ho;Control<Treatment
NUM OF Minimum Sig DiffOUP IDENTIFICATION REPS " (IN ORIG. UNITS)
2**
^6
control6.2512.52550100
33333.3
0.3030.3030.3030.3030.303
% of DIFFERENCECONTROL FROM CONTROL
15.715.715.715.715.7
0.2270.2870.4630.4970.601
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4A-19
eport Date: 11/18/91 " Page: 1
11/12/91 Total # of Samples: 1Run Control K Contant: 0.01348 - Hunter of Entries: 7
Correction: 5.000 Temperature: 21.000 Specific Gravity: 2.650f f
1 Grans Used: 50.00 n: 0.97000 Sample #: 1 Gram Used: 0.00 m: 0.00000
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A-20
aort Date: 11/18/91 Page: 1
: 11/12/91 Total # of Saaples: 1urs Standard Chlorine < Contant: 0.01348 Nuaber of Entries: 7spersant Correction: 5.000 Temperature: 21.000 - Specific Gravity: 2.
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A Q U A S U R V E Y INC.
TEST ORGANISM ACCLIMATION & TRANSPORTATION
DATE: /i fin I_______ . . , . . - . . . QA/QC:
TEST JOB I: 4('133 CLIENT:IN-LAB [X^ ] MOBILE [ ] SATELLITE [ ]
TEST SPECIES; A. p*.<f»t+__________________Total Number of organisms acclimated:AQtJA SURVEY, INC Investigators:1
A. ORGANISMS1. ASI Culture/Holding [Tank /Receiving Log- §1 ;<ffi2. Procured From/Date Received:3. Age Information:_*^
B* RECEIVING WATSR PARAMETERS (Non Cultured Organisms)1. Tempers2, Salinity:3. Alkalinity:
C. HOLDING [ ] CULTURE V I WATER PABAMHTERS1 . Temperature;2. Salinity:3, Alkalinity:4. Water Source!
D. ACCLIMATION [Temperature - Water Type]1. AccTzmarfeion^Chamber Volume (Liters):
^3. Acclimation Water Temperinra4. Acclimation Water Salinity5. Acclimation Commencement - Date6* Change-Over Rate (ml/minute):7. Culture Acclimation Ending-Date:_______ Time:8. _____[ Acclimation Ending-Date:_______ Time:
E. TRANSFER CUSTODY £ TRANSVKR WATER PARAMETERS1. Culture Lab > Test Lab: -Date; nluQt Time;2. Temperature;3. Salinity:4. Alkalinity:5. Culture Lab Technician Initialss-J*- ,jfefrP7 [Transfer]6. Test Lab Technician Initials: (M [Receiving]
REMARKS: (O
* " :' " " fln^nAPI"?C fl 499 Pcint Breeze Pooci - Pemington. New Jersey 08822 • feieohcne (201) 788-370(1 TAxT*20107fi8-(516£-
EPA PROSIT ANALYSIS PROGRAMUSED FOR CALCULATING EC VALUES
Version 1.4
5 hour SRT H. aztecaObserved Adj usted Predicted
Number Number Proportion Proportion Proportiondone. Exposed Resp. Responding Responding Responding
2.5000 20 0 0,0000 0.0000 0.00045.0000 20 2 0.1000 0.1000 0.046810.0000 20 7 0.3500 0.3500 0.492220.0000 20 20 1.0000 1.0000 0.949240.0000 20 20 1.0000 1.0000 0.9995
- Square Heterogeneity s 3.973« 1.003573
i~ma •• 0,181684
li'ameter Estimate Std. Err. 95% Confidence Limitsercept -0.523735 1.088649 ( -2.657487, 1.610018)p@ 5.504067 1.063503 ( 3.419601, 7.588532)
leoretical Spontaneous Response Rate = 0.0000
A-25
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J100 2.0225 5.1809)667 3.1544 6.4630
.8983 3.9795 7.3053
.5355 4.6405 7.9601)826 8.3392 12.19005550 12.7716 21.9047.2353 13.9164 25.54280644 15.7303 32.22375824 19.6229 50.2581
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A-26
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A-28
APPENDIX K - SOIL TOXICITY TEST RESULTS
flR.306.821
RQUn SURV6V, INC
Report: The toxicity of sediment and soil samplesfrom Standard Chlorine of Delaware, DelawareCity, Delaware,
Sponsor: Standard ChlorineGovernor Lea Road, PO Box 319Delaware City, DE 19706
TastingFacility: Aqua Survey, Inc.
499 Point Breeze RoadFlemington, NJ 08822
StudyNumber: 91-189
Report I 1615
DateStudy Started: September 4, 1991
DateStudy Finish: September 25, 1991
DateReported: September 26, 1991
Personnel: Y. Terrell G. MacTyeS. Douglas C. NallyS. Grasso D. KingJ. Fischer C. LawrenceR. Fristrom
_____,*WT1 Scott Douglas ^ " Da'te'Study Director
Page 1 of 3
499 Point Breeze Road » FierungTor., New Jersey O6S22 c "eie one (?O6)7SS-E7CX
flQUfl SURVCV, INC
Summary
Samples of soil and sediment from the Standard Chlorine ofDelaware site in Delaware City, Delaware were collected by Westonpersonnel and shipped to Aqua Survey, Inc.(ASI) on September 4and 5, 1991. Samples were tested for toxicity to earthwormsfEisenia foetida), lettuce fLactuca sativa), and amphipods(Hyallela azteca).
Samples of soil were assessed using both earthworms andlettuce seeds in 14 day and 5 day exposures, respectively.Effects observed were checked for statistical significance usingAnalysis of Variance and Dunnett's Multiple range test. After 14days of exposure, significant mortality of earthworms was notedin samples SLTX-2, 5, 6, and 7 (see Table 1). Significantreduction of germination was observed in samples SLTX-2, 4, 5, 6and 7 (see Table 1).
Samples of sediment were tested for effects on the amphipod,Hvallela azteca using a 10-day static exposure. Significantmortality to Hyallela was observed in samples SDT-4 and SDT-6(see Table II). Surviving amphipods were measured to the nearest,01mm to assess possible sublethal effects - none were noted.
Page 2 of 3
499 Point Breeze Road •Remington. Ne*' Jersey O8S22 » Taiebtccle [9O£)73S-57OO FAX (9
flQUfl SURV€V, INC.
TABLE I
SLTX-1SLTX-2SLTX-4SLTX-5SLTX-6SLTX-7
Summary of Soil Toxicity Tests
Percent Responding
E. foetida L,0%
100%*7%
30%*100%*100%*
sativa33%78%*62%91%*92%*89%*
*denotes statistically significant response at a^O.05,
TABLE 11 Summary of Sediment Toxicity Tests
SDT-1. SDT-2SDT-4SDT-5SDT-6SDT-7
Percent Hyallela
13%21%65%*17%40%*28%
Responding
*denotes statistically significant response at oc<0,05,
following pages contain full data packages/ statisticalanalyses and procedures for each of the species tested.
Page 3 of 3
tMj 495 Point Breeze Road • Remington, Mew Jersey O8S22 « Tefei-.one (9C£)7SS-S7OC
survey
I. Objective
The objective of this test was to determine the toxiceffects of test soils from STANDARD CHLORINE OFDELAWARE when in the environment of terrestrialinvertebrates. The measure of potency is significantmortality of the exposed organisms as compared to acontrol after 14 days.
II. Test material =
Source: Standard ChlorineGovernor Lea RoadPO Box 319Delaware City, DE 19706
Name: SLTX-1 _; . _ _ . . _SLTX-2SLTX-4SLTX-5SLTX-6SLTX-7
Date - - . . * - .Received: September 4, 1991
III. Materials and Methods"LT" ' , • , " ' - •
A. Method
The method employed was a modification of themethod outlined by The United States EnvironmentalProtection Agency, Protocols for Short TermToxicity Screening of Hazardous Waste Sites,EPA/600/3-88/029.
B. Test Organisms
Species - ; - - - : : _ . - - • . -
The test species for this test was Eiseniafoetida, which is a representative terrestrialinvertebrate.
Size/Aae/Phvsical Condition
Animals used in this test were mature1 earthworms>60 days old and appeared to be in good condition.
Page 1 of 5
499 Point Breeze Road • Ffemirtgfon, New Jersey O5-522 « TeJeb-dne1 ;9O3)75S-37OC FA" fifOEJ-fS*'*
RQUfl SURV6V, INC
^Source/Acclimation _
The animals used were obtained from CarolinaBiological (2700 York Road, Burlington, NC) andheld in a peat/soil mixture for at least 10 daysat test temperature prior to testing.
C* Test System
Source of Artificial Soil
A mixture of 10% peat, (Mosser Lee Co., Millston,WI), 20% EPK kaolinite clay, (Felspar Corp.,Edgar, FL) and 70% washed silica sand (CarolinaBiological, 2700 York Road, Burlington, NC) wasused for dilutions.
Temperature
The test temperature was 20+/- 2° C
Test vessels
The test vessels were 1-liter borosilicate glassbeakers covered with a petri plate.
Photoperiod
The test was conducted in continuous light at 50-100 foot candles.
Hvdration Water
T©st soils were brought to 75% of water holdingcapacity using ASI deionized water.
D. Test Design
Test Levels
Three replicates of undiluted test soil weretested and compared to three replicates of anartificial soil.
Page 2 of 5
lUFI 499 Point Breeze Road «• Rerrungfon, New Je-sey O8822 « reiephone (9OS)73c-37OCrJC. BoxS3O5? -Eaton Rocse lculs?cnc 7OS54 •
INC
Control
A control was run concurrently using 100%artificial soil and the same number of organismsas the test soils.
Beginning the Test
Two hundred (200) grains of each test soil andcontrol were added to each of three replicatevessels. Hydration water was added to the nearest0.5 ml using a plastic syringe. Ten animals werechosen randomly and added to each of threereplicate vessels per treatment. Test vesselswere then randomly located in the test chamber.
The test was of 14 days duration.
All test vessels were examined for survival at 7and 14 days. _ ______
Measuring Temperature/Organic Matter Compositionand pH
A temperature record was obtained by taking areading from a thermometer immersed in water nearthe test system twice daily*
The pH of each treatment was measured and recordedat the beginning and end of the test.
Organic matter composition was determined by AshFree Dry Weight Method, Standard Methods, 17th ed.
Reference Toxicant
A standard reference toxicant test with 2-chloroacetamide was performed. The toxicant wasdissolved in deionized water and added with thehydration water to give the requiredconcentrations on a mg/kg basis.
Page 3 of 5
499 Point Breeze Road *Fierrtinston,New Jersey O3S22 « Tsles-.cne (9OS)785-£7OOPO. Box.£3-O5v »-3c:cr. Rouge LcL'sTKa ?C?34 «.Te!-SD o _s (£.00 -0 -468- FAX (5C±\. 923-2
SURV€V. INC
IV. Results
Average survival rarxged from 0 - 100% in all treatments.(See Table I)
The test temperature was maintained at20 +/- 2°C (See Table II).
The pH ranged from 3.6 to 6.5 (see Table II).
Organic matter composition ranged from 0.4 - 7.9%(See Table II).
Significant mortality was observed in samples SLTX-2, 6and 7 after a 7 day exposure. After 14 days, significantmortality was observed in SLTX-2, 5, 6 and 7 (see Table I)In one chamber of SLTX-5, the soil had dried outconsiderably/ possibly leading to increased mortality inthat chamber (see Raw Data).
The 14 day LC50 for the reference toxicant wasdetermined to be 41.9 mg/kg.
V. Source of Documentation
All original data documentation is being maintained at:
Aqua Survey, Inc.499 Point Breeze RoadFlemington, NJ 08822
Page 4 of 5
49? Point Breeze Road • Flerrungfon. New Jersey O3322 * Telephone (90S) 758- S7OO FAX (90S; 7>- = C^ 923-2S3
^^ Table I
CONTROL
SLTX-1
SLTXr-2
SLTX-4
SLTX-5
SLTX-6
SLTX-7
* denotes•Table II
Sample
Control
SLTX-1
SLTX-2
SLTX-4
SLTX-5
SLTX 6
SLTX-7^
Survival at Survival at7 davs 14 davs
100% 100%
100% 100%
0% 0% *
93% 93%
93% 70% jt
0% 0% y
0% 0% fc
statistically significant result
Temperature pH
20.5 - 22.0 4.1 - 6.0
20.5 - 22.0 4.7 - 5.7
20.5 - 22.0 4.8 - 6.1
20.5 - 22.0 3.6 - 4.9
20.5 - 22.0 5.5 - 6.2
20.5 - 22.0 3.8 - 4.1
20.5 - 22.0 5.3 - 6.5
————— HCPUR SUffl
Average Weightat Termination
325 mg
238 mg
NA
207 mg
213 mg
NA
NA
at "<0.05
PercentOrganic Matter
7.9
0.4
1.0
1.2
2.3
3.1
0.7
Page 5 of 5
fiR3Q6829499 Point Sreeze Road • Remington, New Jersey OS822 -« Telep'-!one"(9O8",7oS-S7OO FAX(?O£)7S&-91ccf?CL. Box£3O5? * Be-on Rojpe Loaisic." : 7CS34 * Teiechcne <SOS5 654- cS - FAX foGi 92S-2€"39
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ID: ____________:_________ TEMPERATURE INITIALS TEMPERATURE INITIALS
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AR30683I
'ANDARD CHLORINE EISENIA FOETIDA 14 DAY TEST."•*: STCI£ Transform: ARC SINE (SQUARE ROOT(Y))
u-squar* tast for normality: actual and expected frequencies
BRVAL 01.5 -1.5 to <-0.5 -0.5 to 0.5 >0.5 to 1.5 >1.5
ECTED 1.407 5.082 8.022 5.082 1.407ERVED 0 2 1 5 4 0
culated Chi-Sguare goodness of fit test statistic 10.9833,le Chi-Sguarm value (alpha = 0.01) » 13..277a. PASS normality test. Continue analysis.
ETDARD CHLORINE EISENIA FOETIDA 14 DAY TEST•: STCLE Transform: ARC SINE (SQUARE ROOT(Y))
y test for homogeneity of variancetlatts test for homogeneity of variance
M two tests can not be performed because at least one group haso variance.
ta FAIL to m@©t homogeneity of variance assumption."itional transformations are useless.
f =-
A-2
RR306832
I LE: STANDARD CHLORINE EISENIA FOETIDA 14 DAY TESTSTOLE
: ARC SINE (SQUARE ROOT(Y)) NUMBER OF GROUPS: 7
IDENTIFICATION REP VALUE TRANS VALUE
CONTROL 1 1.0000 1.4120CONTROL 2 1.0000 1.4120CONTROL 3 1.0000 1.4120SLTX-1 1 1.0000 1.4120SLTX-1 2 1.0000 1.4120SLTX-1 3 1.0000 1.4120LSTX-2 1 0.0000 0.1588LSTX-2 2 0.0000 0.1588LSTX-2 3 0.0000 0.1588SLTX-4 1 0.8000 1.1071SLTX-4 2 1.0000 1.4120SLTX-4 3 1.0000 1.4120SLTX-5 1 0.8000 s 1.1071SLTX-5 2 0,8000 1.1071SLTX-5 3 0.5000 0.7854SLTX-6 1 0.0000 0.1588SLTX-6 2 0.0000 0.1588SLTX-6 3 0.0000 t 0.1588SLTX-7 1 0.0000 0.1588SLTX-7 2 0.0000 0.1588SLTX-7 3 0.0000 0.1588
D CHLORINE EISENIA FOETIDA 14 DAY TESTle: STCLE Transform: ARC SINE(SQUARE ROOT(Y))
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 1 of 2
IDENTIFICATION N MIN MAX MEAN
CONTROL 3 1.412 1.412 1.412SLTX-1 3 1.412 1.412 1.412LSTX-2 3 0.159 0.159 0.159SLTX-4 3 1.107 1.412 .I"" 1Y310"SLTX-5 3 0.785 1.107 1.000SLTX-6 3 0.159 0.159 0.159SLTX-7 3 0.159 0.159 0.159
ANDARD CHLORINE EISENIA FOETIDA 14 DAY TESTe: STCLE Transform: ARC SINE(SQUARE ROOT(Y))
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 2 Of 2
ENTIFICATION VARIANCE SD SEM
CONTROL 0.000 0.000 0.000SLTX-1 0.000 0.000 0.000LSTX-2 0.000 0.000 0.000SLTX-4 0 . 031 0 . 176 0 . 102SLTX-5 0 . 035 0 . 186 0 . 107 „ A
A-3 SR3Q6833
SLTX-6 0.000 0.000 0.000SLTX-7 0.000 0.000 0.000
KTDARD CHLORINE EISENIA FOETIDA 14 DAY TESTe: STGLE Transform: ARC SINE(SQUARE ROOT(Y))
ANOVA TABLE
URGE
we©n
thin (Error)al
DF
6
14
20
SS
6.849
0.131
6*980
MS
1.142
0.009
F
126.889
ritical F valu^ « 2.85 (0,05,6,14)F > Critical F REJECT Ho:All groups equal
:«NDARD CHLORINE EISENIA FOETIDA 14 DAY TEST@: STCLE Transform: ARC SINE(SQUARE ROOT(Y))
DUNNETTS TEST - TABLE 1 OF 2 Ho:Control<Treatment
UP
1"3
,.5f
IDENTIFICATION
CONTROLSLTX-1LSTX-2SLTX-4SLTX-5SLTX-6SLTX-7
TRANSFORMEDMEAN
1.4121.4120.1591.3101.0000.1590.159
MEAN CALCULATED INORIGINAL UNITS
1.0001.0000.0000.9330.7000.0000.000
T STAT
0.00016.1791.3125.32016.17916.179
SIG
*
***
mnatt tabl© valu© » 2.53 (1 Tailed Value, P=0.05, df=14,6)
'ANDARD CHLORINE EISENIA FOETIDA 14 DAY TESTi ©: STCLE Transform: ARC SINE(SQUARE ROOT(Y))
DUNNETTS TEST - TABLE 2 OF 2 Ho: ControKTreatment
NUM OF Minimum Sig Dif fROTP IDENTIFICATION REPS (IN ORIG. UNITS)
-
J4
7
CONTROLSLTX-1LSTX-2SLTX-4SLTX-5SLTX-6SLTX-7
3333333
0.0960.0960.0960.0960.0960.096
% of DIFFERENCECONTROL FROM CONTROL
9.69.69.69.69.69.6
0.0001.0000.0670.3001.0001.000
A-4
flR30683U
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A-5
flR306835
A Q U A S U R V E Y INC.
TEST ORGANISM ACCLIMATION & TRANSPORTATION
flQUfi SURV€V, INC
DATE: • ' ______ . * , QA/QC:TEST JOB *: Ql-ffft CLIENT:IN-LAB I/"I MOBILE [ ] SATELLITE [ 1
TEST SPECIES: £-- t*uCt£/sLv________________Total Number of organisms acclimated:_AQUA SURVEY, INC Investigator£T7jVl\
\sA. ORGANISMS
x1. ASI Culture/Holding [Tank /Receiving Log f ] : ?2. Procured From/Date Receied: Q(-CMl3. Age Information : v?
B. RECEIVING WATER PARAMETERS (Non Cultured Organisms)1 . Temperature : 3 H .o___2. Salinity: .3. Alkalinity: _
C, HOLDING [ /I CULTURE [ ] WATER PARAMETERS1 . Temperature : > 5"- Q* "2. Salinity: ~" . ____3. Alkalinity: _4- Water Source:
ACCLIMATION [Temperature - Water Type]1„ -Acclimation Chamber Volume (Liters):2. Acclimatrtosi--W ter Type:3. Acclimation4. Acclimation Water SalinTt5. Acclimation Commencement -6. Change-Over Rate (ml/minute):7. Culture Acclimation Ending-Date:8* ______ Acclimation Ending-Date:
E. TRANSFER CUSTODY & TRANSFER WATER PARAMETERS1. Culture Lab > Test Lab: -Date; 7 *0 '1 ( Time:_2, Temperature; ,-X . ^ ""3. Salinity: ' ^ 1 - *4. Alkalinity: ^ ^5. Culture Lab Technician InitialsV xb/ [Transfer]6. Test Lab Technician Initials: T /Jy>f7 / [Receiving]
REMARKS: ( dm;, Pj fL.U rf Q
A-6g p o n
499 Point Breeze Rood * Remington, New Jersey 08822 • Telephone (201) 788-8700 !=& (20 )738- 165
ORGANIC HATTER CCNPCSITIO* OETESMENATIOM WORKSHEET
le #: calibration? YES NO (Circle one) *
i i Oven: Temperature:
;ti« Furn
a, .s ID
CA
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ace: Tewerature:
1Crucible ID
1
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Crucible Mass(flB)
// Tf-Jf"• fe
R.il?7H- <f'79/'/Z / 7
Crucible * Sonple
Z/ ' /27.72**157 -3Z.Q3f7J "Z.S ""
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' nician Initials: ______:____ Date:
s X Organic Hatter » (Wet sample * Crucible) * (Dry sample * Crucible)(Wet sample * Crucible) - Crucible
uier SQIU Mft icftreR a/e MotsroRS
13.. _ . -» t 1 ji yO jj\ ^ T
-2
-5 n.aa 3.1o,7A-7
flR306837
'C. NUMBER NUMBER PERCENT BINOMIALDEAD DEAD PROS. (%)18 90 2.012253E-027 35 13.1588
.. .5 20 0 0 9.536743E-05J.75 20 0 0 9.536743E-05375 20 0 0 9.536743E-05: BINOMIAL TEST SHOWS THAT 17.5 AND 70 CAN BEAS STATISCALLY SOUND CONSERVATIVE 95 PERCENT
>NFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVELOCIATED WITH THESE LIMITS IS 99.97978 PERCENT.APPROXIMATE LC50 FOR THIS SET OF DATA IS 41.62775
>»»»RESULTS CALCULATED USING THE MOVING AVERAGE METHODG LC50 95 PERCENT CONFIDENCE LIMITS7.6027S2E-02 41.30789 35.0066
50.09094»»RESULTS CALCULATED USING THE PROBIT METHOD
LoJRATIONS G H GOODNESS OF FIT PROBABILITY24 .1723734 1 .9488084
>PE - 6.118149PERCENT CONFIDENCE LIMITS » 3.578023 AND 8.658274
0 - (41. 886921 -———————\ -————————\PERCENT CONFIDENCE" LIMITS - 134.91167J AND 150.42877J
- 17.44894 ———————5 PERCENT CONFIDENCE LIMITS - 9.081364 AND 23.35941
A-8
AR306838
.^ - *** **G*. IMC a j Q>Job # : ____________;_____________ EARTHWORM DAILY QSSgRVATZGMS Start d«t» : y -X. — y >
Stirt tine :
TEMPERATURE INITIALS TEMPERATURE INITIALS
DAY 0: __/ C gtfj DAY 8:
DAY 1:43 /Al. SlU DAY 9 f/A>,6, Cj/1
DAY 2:36o/Jli >!/; DAY
DAY 3s3J./j2J_ _2fflLV_ DAY
DAY JH3.D3/ all 3J& DAY
DAY 5 0.?/ > 9jt9 DAY
DAY 6;Jlg,5/gtJl SSL DAY
DAY
NOTES AMD OBSERVATIONS:
A-9
R30S839
flQUR SURV€V, INC
I. Objective
The objective of this study was to determine the toxiceffects of test soil from the STANDARD CHLORINE OFDELAWARE site on seed germination when in theenvironment of terrestrial plants. The measure ofpotency is that initial concentration of test soil(compared to a control) which produces significantreduction in germination of the exposed seeds after 120hours.
II. Test material
Source: Standard ChlorineGovernor Lea Road, PO Box 319Delaware City, DE 19706
Name: SLTX - 1SLTX - 2SLTX - 4SLTX - 5SLTX - 6SLTX -7
DateReceived: September 4, 1991
III. Materials and Methods
A. Method
The method employed was a modification of themethod outlined by The United States EnvironmentalProtection Agency, Protocols for Short TermToxicity Screening of Hazardous Waste Sites,SPA/600/3-88/029.
B. Test Organisms
Species -
The test species for this test was Lactuca sativa.which is a representative terrestrial plant.
Size/Age/Physical Condition
Seeds used in this test were of this year's lotculled for size and appeared to be in goodcondition.
Page 1 of 5
499 Point Breeds Road • F!errJngicn,New Jersey O3£22 * Telephone C9O£)7&8-37OC ?AK
flQUfl SURV€Y, INC.
Source/Acclimation
The seeds used were obtained from Harris Seeds, 60Saginaw Drive, Rochester, NY; Lot #1664;Buttercrunch variety #506; packaged for 1991.
C. Test System
Source of Artificial Soil :
Washed silica sand from Carolina Biological, (2700York Road, Burlington, NC) was used as a controlmedium.
Temperature '~'~~-'
The test temperature was 24+/- 2° C
Test vessels " ' •
The test vessels were 15xl50-mm plastic petridishes placed in 12" resealable plastic bags.
Photoperiod
The first 48 hours of testing were in totaldarkness, followed by a 16-hour light / 8-hourdark photoperiod at 400 +/" 40 foot candles.
Hvdration Water
Hydration of test soils was brought to 85% ofwater holding capacity using ASI deionized water.
D. Test Design
Test Levels ^ , .^ _
Three replicates of undiluted test soil weretested and compared to three replicates of anartificial control soil.
Page 2 of 5
499 Point Breeze Roac • Remington, New Jersey O8S22 « re.:ephoner:(?O3)?3S-37CC
flQUR SURV€V, INC
Control
A control w.as run concurrently using 100%artificial soil and the same number of organismsas the test concentrations.
Beginning the Test
One hundred (100) grams of each test soil andcontrol were added to each of 3 replicatechambers. Hydration water was added to thenearest 0.5 ml using a plastic syringe. Fortyseeds were chosen randomly and added to each ofthree replicate chambers per treatment and gentlycovered with silica sand. Each plate was thenplaced in a resealable plastic bag and randomlylocated in the exposure chamber.
The test was of 120 hours duration.
All test vessels were examined for germination at120 hours. The seedlings were removed singly fromthe test chamber and enumerated.
Measuring Temperature and pH
The temperature of a single replicate of eachtreatment was measured for temperature at thebeginning of the test and daily thereafter.
The pH of each treatment was measured and recordedat the beginning and end of the test.
E. Reference Toxicant
A standard reference toxicant test with 2-chloroacetamide was performed. The toxicant wasdissolved in deionized water and added with thehydration water to give the requiredconcentrations on a mg/kg basis.
xnPage 3 of 5
49? Point Breeze Roao* • Fierrilngtor.. New jersey OSS22 * Telephone (9O2/735-87OCO ^ v s Di"\' [-"Itt * f\! '"el«-p •"•*"«• "TO1 C G>> _ *" .1y-.,» . %,,—, . /"C ••V v1* i.Cy /'Of "->\/ f^LJf^- £*• ~ f**\ s*.n "B 1-*— *-»i KOuge I.OU.S.G. ;•» /-« 0c4 * : :S—rtOP.S v-^^. 5Ofe-4co4 r/-.A ,C -*4._, r_: - c3'y
flQUfi SURV6V, INC
IV. Results
Average germination ranged from 8 - 92% in all treatments.(see Table I).
The test solution temperature was maintained at24 +/- 2°C (See Table II) .
The pH ranged from 3.3 to 7.4 (see Table II).
Significant reduction (as determined by Analysis ofVariance) in germination was observed in all samples exceptSLTX - 1.
The 120-hour EC50 for the reference toxicant wasdetermined to be 8.04 mg/kg. This compares to anacceptable range according to the USEPA of6.64-14.2 mg/kg (see Appendix for data).
V. Source of Documentation
All original data documentation is being maintained at
Aqua Survey, Inc.499 Point Breeze RoadFlemington, NJ 08822
Page 4 of 5
a/i 499 Point Breeze Road •Fieraingtoa tvew Jersey OSS22 * Telephone (9O3)7S3-£7OC FAX (9Oc£tPi O y R3O C » 5" " P- 'J P I O"? !:V - "'f'-Z s T ]A—S——%e. -'C"->/-N' i = ' /!AC=j; E'iv '=."•* ,"N- CT< OPr- • -'- "-~f ~ !_;•_? w%~ . i-> - •v. . i . fj 's i-*~k~,3,- .. i>_ , •\_*-i oinf « sSrtw-^, ir ^ ,fc i c *~r *<&f- —'Oic" 4CO*-i' rV-v/v .O'fc i- y ' .t
flQUfl SURV6V. INC.
Table ...1
CONTROL
SLTX - 1
SLTX - 2
SLTX - 4
SLTX - 5
SLTX - 6
SLTX - 7
* Samples
Table II
CONTROL
SLTX - 1
SLTX - 2
SLTX - 4
SLTX - 5
SLTX - 6
SLTX - 7
Average Germination Rate (percent)
ReplicateA B C
90 88 98
85 68 78
28 8 30
33 45 55
3 5 20
15 5 3
13 8 13
which showed significant effect at
Lettuce Seed Physical Chemical
Temperature °C pH
23.0 - 24.5 6.7 - 7
23.0 - 24.5 4.3 - 4
23.0 - 24.5 4.3 - 4
23.0 - 24.5 3.3 - 3
23.5 - 24.5 5.3 - 5
23.0 - 24.5 3.5 - 3
23.0 - 24.5 4.7 - 5
Average
92
77
22 *
38 *
9 *
8 *
11 *
a < 0.5.
Data
.4
.7
.8
.6
.5
.8
.3
Page 5 of 5
•Ml 499 Point Breez© Rocd »=Iertington. New Jersey C3S2£ * 7e:ep~one (9C£)7oc-S7OO FAX (vofl ft 5&& 6 8 k kP.O. BcxS3O59 *Se*cr. Rouse Lculsscnc 7
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N
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:ANDARD CHLORINE LACTUCA SATIVA 5 DAY GERMINATION. a: STCLL Transform: ARC SINE (SQUARE ROOT(Y))
tapiro Wilks test for normality
0.205
0.958
ntical W (P = 0.05) (n = 21) « 0.908ritical W (P * 0.01} (n » 21) = 0.873
ita PASS normality tmst at P O.Ol level. Continue analysis
CANDARD CHLORINE LACTUCA SATIVA 5 DAY GERMINATIONPa: STCLL Transform: ARC SINE (SQUARE ROOT(Y))
test for homogeneity of variance
a culated B statistic 2.45Chi-square value «• 16.81 (alpha = 0.01)
able Chi~square value « 12.59 (alpha = 0.05)
y rage df used in calculation =^> df (avg n - 1) - 2.00'.ed for Chi-sguar© table value => df (igroups-l) = 6
.-a PASS homogeneity test at 0.01 level. Continue analysis.ir"E: If groups have unequal replicate sizes the average replicate size is
used to calculate the B statistic (see above).
'I LE: STANDARD CHLORINE LACTUCA SATIVA 5 DAY GERMINATIONI E: STCLLXANSFQRM: ARC SINE (SQUARE ROOT(Y)) NUMBER OF GROUPS:
R*' IDENTIFICATION REP VALUE TRANS VALUE
3 CONTROL 1 0.9000 1.24903 CONTROL 2 0.8800 1.21711 CONTROL 3 0.9800 1.42892 SLTX-1 1 0.8500 1.1731: SLTX-1 2 0.6800 0.9695: SLTX-1 3 0.7800 1.08263 SLTX-2 1 0.2800 0.5576? SLTX-2 2 0.0800 0.2868: SLTX-2 3 0.3000 0.5796^ SLTX-4 1 0.3300 0.61194 , SLTX-4 2 0.4500 0.7353f SLTX-4 3 0.5500 0.8355! SLTX-5 1 0,0300 0.1741b SLTX-5 2 0.0500 0.22555 SLTX-5 3 0.2000 0.4636« SLTX-6 1 0.1500 0.3977
A-I fiR3G68i*6
SLTX-6 2 0.0500 0.2255SLTX-6 3 0.0300 0.1741SLTX-7 1 0.1300 0.3689SLTX-7 2 0.0800 0.2868SLTX-7 3 0.1300 0.3 689
:ANDARD CHLORINE LACTUCA SATIVA 5 DAY GERMINATIONe:STCLL Transform: ARC SINE(SQUARE ROOT(Y))
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 1 of 2
IDENTIFICATION N MIN MAX MEAN
CONTROL 3 1.217 1.429 1.298SLTX-1 3 0.970 1.173 1.075SLTX-2 3 0.287 0.580 0.475SLTX-4 3 0.612 0.835 0.728SLTX-5 3 0.174 0.464 "0.288SLTX-6 3 0.174 0.398 0.266SLTX-7 3 0.287 0.369 0.341
ANDARD CHLORINE LACTUCA SATIVA 5 DAY GERMINATIONe: STCLL Transform: ARC SINE (SQUARE ROOT(Y))
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 2 of 2
ENTIFI CATION VARIANCE SD SEM
CONTROL 0.013 0.114 0.066SLTX-1 0.010 0.102 0.059SLTX-2 0.027 0.163 0.094SLTX-4 0.013 0.112 0.065SLTX-5 0.024 0.154 0.089SLTX-6 0.014 0.117 0.068SLTX-7 0.002 0.047 6.027
"JTDARD CHLORINE LACTUCA SATIVA 5 DAY GERMINATION.e: STCLL Transform: ARC SINE(SQUARE ROOT(Y))
ANOVA TABLE
URGE
:ween
thin (Error)
:al
DF
6
14
20
ss3.033
0.205
3.238
MS
0.505
"0.015
F
33.667
.___cal F value = 2.85 (0.05,6,14)Since F > Critical F 'REJECT Ho:All groups equal
A-2 flR3068U7
I'ANDARD CHLORINE LACTOCA SATIVA 5 DAY GERMINATIONile: STCLL Transform: ARC SINE(SQUARE RQOT(Y))
DUNNETTS TEST - TABLE 1 OF 2 HorControKTreatmentmF W
1**
•*5
' IDENTIFICATION
CONTROLSLTX-1SLTX-2SLTX-4SLTX-5SLTX-6SLTX-7
TRANSFORMEDMEAN
"1.2981.0750.4750.7280.2880.2660.341
MEAN CALCULATED INORIGINAL UNITS
0.9200.7700.2200.4430.0930.0770*113
T STAT
2.2338.2375.70810.10610.3269.568
SIG
*****
unnett table value = 2.53 (1 Tailed Value, P O.05, df=14,6)
PANDARD CHLORINE LACTUCA SATIVA 5 DAY GERMINATIONi e: STCLL Transform: ARC SINE(SQUARE ROOT(Y))
DUNNETTS TEST - TABLE 2 OF 2 Ho: ControKTreatment
NUM OF Minimum Sig DiffkoUP IDENTIFICATION REPS (IN ORIG. UNITS)
CONTROLSLTX-1
3 SLTX-24 _ SLTX-4^ SLTX-5V SLTX-6
7 SLTX-7
3333333
0.1790.1790.1790.1790.1790.179
% of DIFFERENCECONTROL FROM CONTROL
19.519.519.519.519.519.5
0.150'0.7000.4770.8270.8430.807
A-3
JOtt *s_____________ AQUA SOIVEY; 1HC. IMIT1ATIOH DATE: f^£—/- LETTUCg SEED GgRHlHATlOM INITIATION TIME;" 3;™ ™ ww* « ™»-
SAHPLE I.D.
CONTROL
TEHPERATURE
P«
&.TX-2.
TIHPERATURE
P«
Si-TX-V
TEMPERATURE
P«
TEHPERATURE
pa
TEMPERATURE
UITIALS
QA/OC
RIP Count
3.4.
TIH£ (HOURS)24 48 72 96 120
B.i*
$4.55.5"
53.5
23.0
$3.0
5X3.0
ZD
53.5
Z7/
1A
23.0
53
GeminationCount
13/YASi
53
T
9
MOTES and OftSERVATlOHS:
A-4
GeminationRate <X>
77?
,30
33,
5
92
0
; — L
AR3068U9
JOB *:_____________________ AQUA SURVEY, IMC. INITIATION DATE; 9S^ -5LETTUCE SEED GERMINATION INITIATION
MOTES and OBSERVATIONS:r(Ltittfsfrwa df&P?f-V
W& rfj*> (1 T
A-5 AR306850
3.-40* *s qi*-VSR_______ AQUA SURVEY, INC. INITIATION DATE; L?-L>-
_J_____________ LETTUCE SEED GERMINATION INITIATION TIMS; -V,.5Opu*
SJ04W.E 1.0.
CONTROL
TEHPIRATUftE
P«
•pp*
TIMPERATURI . . ..„ .„. , , ...... .... „ .. , „._ . ^
PH
TEMPiRATURE
PH
TEMPERATURE
TEMPERATURE
PM
TEHPIRATlǤ
PH
INITIALS
OA/OC
REPS«*dCount
40
3M-5
MD
40
4040
TIME CHOIRS)24 48 72 96 120
40
40au.5--* -2
409S,\
JJ
J/
G«n«i nationCount
-55"34=
373330
3.5-54=34
25-
53
VC
0
UOTIS and OKERVATIOUS:
terminationRate «5
75
95
7?
..3
3
00
flR30685!
SAMPLE ID: SRT, Lettuce
BINOMIALNUMBER - NUMBER PERCENT PROBABILITY
CONCENTRATION EXPOSED AFFECTED AFFECTED (%)34.3000 120 120 100.0000000 0.000000017.2000 120 116 96.6666600 0.00000008.6000 120 31 25.8333300 0.00000004.3000 120 15 12*5000000 0.00000002.2000 120 20 16.6666700 0.0000000
RESULTS
' 9 5METHOD SPAN G EC50 CONFIDENCE LIMITS
BINOMIAL 10.565930 8.600001 - 17.200000
MOVING AVERAGE 4 . 0.012960 8.040998 7.234806 - 8.921089•
PROBIT 1.454574PROBIT (........................NOT VALID.......................)
INTERPRETATION OF STATISTICS
The order of preference of results is:BEST: Probit MethodBETTER: Moving AverageGOOD: BinomialGOOD: Graphic Interpolation (Graph drawn by hand)
assuming all are statistically valid.To Determine if a method has given valid results:GRAPHICAL: Always validBINOMIAL: The program will tell you if this is not valid.MOVING AVERAGE: The number of spans needs to be no less than one
lower than the number of concentrations tested(excluding control).
PROBIT: The program will tell you if this is not valid
A-7
• fl/?306852
SETTransform: NO TRANSFORMATION
apiro Wilks test for normality
62.667
0.925
x-tical W (P * 0.05) (n - 15) - 0.881itical W (p * 0.01) (n » IS) = 0.835
ta PASS normality test at P*0.01 level. Continue analysis,
F lettuce SRT"E: icfsrt "Transform: NO TRANSFORMATIONrtletts test for homogeneity of variance
sulatsd B statistic » 10.01ble Chi-square value ~ 13.28 (alpha = 0.01)ble Chi-square value 9.49 (alpha = 0.05)
rage df used in calculation ^^> df (avg n - 1) » 2.00ed for Chi-sgaare table value =s> df (#groups-l) « 4
a PASS homogeneity test at 0.01 level. Continue analysis.
E: If groups have unequal replicate sizes the average replicate size isused to calculate the B statistic (see above) .
LE: ICF lettuce SRT'_E: icfsrtANSFORM: NO TRANSFORMATION NUMBER OF GROUPS: 5
if IDENTIFICATION REP VALUE TRANS VALUE
L control 1 35.0000 35.0000L control 2 36.0000 36.0000L control 3 36.0000 36.00002 2.2 1 37.0000 37.00002 2.2 2 33.0000 33.00002 2.2 3 30.0000 30.00003 4.3 1 35.0000 35.00003 4.3 2 36.0000 36.00003 4 . 3 3 34.0000 34.00004 8.6 1 25.0000 25.00004 • 8.6 2 31.0000 31.00004 8.6 3 33.0000 33.00005 17.2 1 1.0000 1.00005 17.2 2 2.0000 2.00005 17.2 3 1.0000 1.0000
117306853
lettuce SRT2: Transform: NO TRANSFORMATION
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 1 of 2
JP IDENTIFICATION N MIN" MAX MEAN
control 3 35.000 36.000 35.6672.2 3 30.000 37.000 33.3334.3 3 34.000 36.000 35.0008.6 3 25.000 33.000 29.66717.2 3 1.000 2.000 1.333
lettuce SRTi: icfsrt Transform: NO TRANSFORMATION
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 2 Of 2
IDENTIFICATION VARIANCE SD SEM
control 0.333 0.577 0.3332.2 12*333 3.512 2.0284.3 1.000 1.000 0.5778.6 17.333 4.163 2.40417.2 0.333 0.577 0.333
lettuce SRT>: icfsrt Transform: NO TRANSFORMATION .
ANOVA TABLE =
JRCE DF SS MS F
/een ; 4 2535.333 633.833 101.138
in (Error) 10 62.667 6.267
il 14 2598*000
ritical F value = 3.48 (0.05,4,10)since F > Critical F REJECT Ho:All groups equal
lettuce SRTLe: icfsrt Transform: NO TRANSFORMATION
[UNNETTS TEST - TABLE 1 OF 2 Ho:Control<Treatment
TRANSFORMED MEAN CALCULATED INJP IDENTIFICATION MEAN ORIGINAL UNITS T STAT SIG
1 control 35.667 35.667
'
4.3 35.000 35.000 0.3268.6 29.667 29.667 2.935 *17.2 1.333 1.333 16.797 *
onett tabl© value » 2.47 (1 Tailed Value, P=0.05, df-10,-4)
lettuca SRTa: Transform: NO TRANSFORMATION
TABLE 2 OF 2 Ho:Control<Treatment
NDM OF Minimum Sig DiffI JP IDENTIFICATION REPS (IN ORIG. UNITS)i
•j4
control2.24.38.617.2
33333
5.0495.0495.0495.049
% Of DIFFERENCECONTROL FROM CONTROL
14.214.214.214.2 '
2.3330.6676.00034.333
A-10
flR3Q6855
SURV6V, INC
•1/1
I. Objective
The objective of this test was to determine the toxiceffects of test sediments froia the STANDARD CHLORINE ofDELAWARE site when in the environment of benthicinvertebrates. The measure of potency is significantreduction in survival of exposed organisms as compared toa control after ten days.
II. Test material
Source: Standard ChlorineGovernor Lea Road, PO Box 319Delaware City, DE 19706
Name: SDT - 2SDT - 3SDT - 4SDT - 5SDT - 6SDT - 9
DateReceived: September 4, 1991
III* Materials and Methods
A. Method
The method employed was a modification of themethod outlined by American Society of TestingMaterials, Standard Guide for Conducting SedimentToxicity Tests with Freshwater Invertebrates,E1383-90.
B. Test Organisms
Species
The test species for this test was Hyallela azteca,which is a representative benthic invertebrate.
Size/Age/Physical Condition
Animals used in this test were immature amphipods,<14 days old and appeared to be in good condition.
Page 1 of 7
49? Poin: Breeze Road • Fleming ton. New Jersey O3322 • Telephone '9O3)73S-S7OGP.O. 36x £3O5? • B"tcr Rouse Lcufs?c c 70 54 •T5:$3rio-™e"*''ft'-
-flQUfl SURV€V, INC
Source/Accl imation
The animals used were obtained from AquaticResearch Organisms in Portsmouth, New Hampshire andwere held for 1 week prior to testing.
Test System
Source of Dilution Water
Dilution water was moderately hard reconstitutedculture water obtained from Aquatic ResearchOrganisms.
Temperature
The test temperature was 20+/~ 2° C
Test vessels
The test vessels were 1-liter borosilicate glassbeakers covered with a petri plate and gentlyaerated through glass tipped aeration lines.
Photoperiod
The test was conducted on a 16-hour light/8-hourdark photoperiod.
D. Test Design
Test Levels
Five replicates of undiluted test sediment weretested and compared to five replicates of controlsediment.
Page 2 of 7
495 Point Breeze Rocd • Flerrungfor,. New Jersey C3522 * Teleoncne (9O3;7SS-S7CORO. Bex S3D59 * Bc*CP. I OJ s LcL.'rSicncr 70 54 c "Triec one SC/C" 654- 684- FAX
flQUfl SURVeY, IN
Control
A control, sediment was obtained from Spruce RunReservoir in Annandale, New Jersey. The controlswere run using 100% control sediment and the samenumber of organisms as the test concentrations.
Beginning the Test
The test was initiated by placing approximately200 cm3 of test sediment into each of five replicatetest vessels and filling the vessels with dilutionwater. The system was allowed to settle undergentle aeration overnight. After settling, twentyanimals were chosen at random and gently added tothe test vessels.
The test was of 10 days duration.
All test vessels were examined for survival at 10days.
Water Quality Measurements
Dissolved oxygen, pH, temperature and conductivitywere determined at the start and end of the test inevery test vessel. Daily measurements of theseparameters were taken in one test vessel from eachtreatment. Total alkalinity and total hardnesswere measured in one test vessel from eachtreatment at the start and every test vessel at theend of the test*
Feeding
Animals were fed three times weekly on a diet ofcrushed Tetra Min and cereal flakes as required bythe ASTM guidance.
Grain Size
Particle size distribution was determined usingStandard Method for Particle-Size Analysis of SoilsASTM Designation: D 422 - 63 (Reapproved 1972)American National Standard A37 145-1972 ApprovedMarch 2, 1972 by American National StandardInstitute.
i/1Page 3 of 7
499 Point Breeze Road • Fierntngtor. New Jersey OSS22 « 7eiep or:er(9O£)7eS-£7CC?>O. BcxS3O5r * Baron Rojge Lo :sic.->a 7O3S4 « Ta:ec-or.e: OO} 54-46£4 FAX :5C4 ?23-2£3
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E. Reference Toxicant
A standard reference toxicant test with SodiumDodecyl Sulfate was performed. The toxicant wasdissolved in deionized water and mixed with culturewater to achieve the desired concentration. Nosubstrate was provided.
IV. Results
Average survival ranged from 20 to 100% in all replicates (SeeTable I). Analysis of variance testing with Dunnett•sprocedure indicated significant reduction of survival insamples SDT - 4 and SDT - 6 as compared to the controls. Nosignificant reduction in survival was noted in other samples.
No significant differences in length were noted amongsurviving amphipods.
The test solution temperature was maintained at20 +/" 2°C (See Table III) .
Th pH ranged from 5.3 to 8.4 (see Table'111).
Conductivity ranged from 600 to 800 umhos/cm. Dissolvedoxygen was kept at or above 8.1 mg/L in all test vessels.Alkalinity ranged from 4 to 112 mg/L. Hardness ranged from108 to 180 rng/1. (See Table III).
Particle size distribution is outlined in Table IV,
The 48 hour LC50 for the reference toxicant wasdetermined to be 9.5 mg/L.
V. Source of Documentation
All original data documentation is being maintained at
Aqua Survey, Inc.499 Point Breeze RoadFlemington, NJ 08822
Page 4 of 7
499 Poin1 Breeze Road • FertfnQfcn, New je-sey O8S22 « T&ec r-e (9OS)7SS-S7OC FAX ?HO. Sox83O59 * Scron Ro-Jpe LoL;s!crtc ~OSS = Isorcrs (SOO; £ 5.4-4634 =AX '5CX", *23-2
flQUfi SUBV€V, INC.
Table I Percent Survival of Amphipods after 10 Day Exposure
Sample
CONTROL
SDT - 2
SDT - 3
SDT - 4
SDT - 5
SDT - 6
SDT - 9
* Samples
Table II
Sample >
CONTROL
SDT - 2
SDT - 3
SDT - 4
SDT - 5
SDT - 6
SDT - 9
ReplicateA B C D E Averacre
80 95 85 65 90 83
90 95 80 95 75 87
85 70 75 90 75 79
35 55 20 .35 30 35 *
90 100 85 75 ,65 83
80 55 25 80 60 60 *
60 70 75 75 80 72
which showed significant effects at a < 0.50.
Hyallela Growth Summary
lean Length S
1.85 0,19
1.92 0.08
1.96 0.09
1.72 0.16
1.98 0.18
1.90 0.19
1.76 0.15
Page 5 of 7
499 Point Breeze Roac • Fierrjngtor, New Jersey. O3S22 < "eSei oriel (9OS}7SS-S7OO FAXv"il>?33-Oi(ft6 0
flQUfi SURV6Y. INC
Table III Physical Chemical Readings for Amphipod Test
Sample
CONTROL
SDT - 2
SDT - 3
SDT - 4
SDT - 5
SDT - 6
SDT - 9
Dissolved pHOxygen
8.1-9.4 7.6-8.4
8.1-9.4 7.2-8.3
8.1-9.4 7.1-8.3
8.1-9.4 6.3-8.3
8.1-9.4 5.5-8.0
8.1-9.4 5.3-7.7
8.1-9.3 7.2-8.2
Temperature Conductivity(°C) (umhos/cm)
19.0-21.0 600-700
19,0-20.5 600-700
18.5-20.5 600-700
19.5-20.5 550-650
19.5-20.5 600-650
19.0-20.5 600-700
19.5-21.0 700-800
§aS5pla.
CONTROL
SDT - 2
SDT - 3
SDT - 4
SDT - 5
SDT - 6
SDT - 9
TotalAlkalinity
Totalfmg/1) Hardness (mg/1)
60-112 148-180
52-76
52-76
12-48
4-68
8-68
56-88
136-148
132-152
108-124
116-132
116-140
148-152
Page 6 of 7
•UP I 499 Point Breeze £ocd • FiterrSnctopi.New ._e*sey OS-522 « Telephone (9O£)783-3?OC =A>:______=O 5oxS3-O5y *Bc-cr Rouge lc-:s!anc 73S54 * T erho-.e (SOC 654-4*54"r?AX"'5C
simvev, INC.
A. Physical Testing
Test sediment samples were cored from six locations inthe project area. There were no visually observedstrata formations. Grain size and water contentdeterminations were made for each test sediment coringsite and the control sediment.
Grain size distribution is defined by the followingcriteria: •
Sand > or = to 0.0625 mm _Silt < 0.0625 but > 0.0039 mmClay < d.0039 mm
See Table 4 for Grain Size and Percent Moistureresults. - -
Table IV Results of Grain Size and MoistureContent Analysis
Sample
SDT-2
SDT-3
SDT-4
SDT-5
SDT-6
SDT-9
Control
Sand
95.0
17.0
41.0
0.0
0.0
0.0
66.0
Silt
3.0
51.0
39.0
47.0
48.0
55.0
22.0
Clay
2.0
32.0
20.0
53.0
52.0
45.0
12.0
Moist.
18.8
42.3
35.4
47.9
49.7
58.9
57.6
Page 7 of 7
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4R306863
CHLORINE HYALELLA AZTECA 10 DAY SURVIVALSTCLH Transform: ARC SINE (SQUARE ROOT(Y))
Wilks test for normality
0.741
0.983
itical W (P = 0.05) (n = 35) = 0.934itical W (P » 0.01) (n = 35) - 0.910
ta PASS normality test at P^O.Ol level. Continue analysis,
ANDARD CHLORINE HYALELLA AZTECA 10 DAY SURVIVAL,STCLH Transform: ARC SINE(SQUARE ROOT(Y))
rtletts test for homogeneity of variance
ulated B statistic = 4 * 5 9ble Chi-square value = 16.81 (alpha - 0.01)
Chi-square value =_ 12.59 (alpha = 0.05)
-rage df used in calculation ==> df (avg n - 1) = 4.00ed for Chi-square table value => df (#groups-l) - 6
> homogeneity test at 0.01 level. Continue analysis.
E: If groups have unequal replicate sizes the average replicate size isused to calculate the B statistic (see above).
A-l
C_LE: STANDARD CHLORINE HYALELLA AZTECA 10 DAY SURVIVALCLE: STCLH
[; ARC SINE (SQUARE ROOT(Y)) NUMBER OF GROUPS: 7
IDENTIFICATION REP VALUE TRANS VALUE
CONTROL 1 0.8000 1.1071CONTROL 2 0.9500 1.3453CONTROL 3 0.8500 1.1731CONTROL 4 0.6500 " 0.9377CONTROL 5 0.9000 1.2490SDT-2 1 0.9000 1.2490SDT-2 2 0.9500 1.3453SDT-2 3 0.8000 1.1071SDT-2 4 0.9500 1.3453SDT-2 5 0.7500 1.0472SDT-3 1 0.8500 1.1731SDT-3 2 0.7000 0.9912SDT-3 3 0.7500 1.0472SDT-3 4 0.9500 1.3453SDT-3 5 0.7500 1.0472SDT-4 1 0.3500 0.6331SDT-4 2 0.5500 0.8355SDT-4 3 0.2000 0.4636SDT-4 4 0.3500 0.6331SDT-4 5 0.3000 0.5796SDT-5 1 0.9000 1.2490SDT-5 2 1.0000 1.4588SDT-5 3 0.8500 1.1731SDT-5 4 0.7500 1.0472SDT-5 5 0.6500 0.9377SDT-6 1 0.8000 1.1071SDT-6 2 0.5500 0.8355SDT-6 3 p.2500 0.5236SDT-6 4 - 0.8000 1.1071SDT-6 5 0.6000 0.8861SDT-9 1 0.6000 0.8861SDT-9 2 0.7000 0.9912SDT-9 3 0.7500 1.0472SDT-9 4 0.7500 1.0472SDT-9 5 0.8000 1.1071
CHLORINE HYALELLA AZTECA 10 DAY SURVIVALSTCLH Transform: ARC SINE (SQUARE ROOT(Y))
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 1 of 2
IDENTIFICATION N MIN MAX MEAN
CONTROL 5 0.938 1.345 1.162SDT-2 5 1*047 1.345 1.219SDT-3 5 0*991 1.345 1.121SDT-4 5 0.464 0.835 0.629SDT-5 5 0.938 1.459 1.173SDT-6 5 0.524 1.107 0.892SDT-9 5 0.886 1.107 1.016
A-2 SR3Q6865
r*NDARD CHLORINE HYALELLA AZTECA 10 DAY SURVIVALi e: STCLH _ Transform: ARC SINE (SQUARE ROOT(Y))' 1i ^i
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 2 of 2
IDENTIFICATION VARIANCE SD SEM
L CONTROL 0.024 0.154 0.069> SDT-2 0.019 0.137 0.061
SDT-3 0.020 0.142 0.064SDT-4 0.018 0.135 0.060
> SDT-5 0.04 0 0.199 0,0895 SDT-6 0,058 0.241 0.108
SDT-9 0.007 0.083 0.037
HOARD CHLORINE HYALELLA A2TECA 10 DAY SURVIVALe: STCLH Transform: ARC SINE(SQUARE ROOT(Y))
ANOVA TABLE
JURCE DF SS MS .. F
ween 6 1.310 0.218 8.385
.thin (Error) 28 0.741 0.02634 2.051
ritical F value « 2.45 (0.05,6,28)ince F > Critical F REJECT Ho:All groups equal
_J*DARD CHLORINE HYALELLA AZTECA 10 DAY SURVIVALle: STCLH Transform: ARC SINE(SQUARE ROOT(Y))
DUNNETTS TEST - TABLE 1 OF 2 ._ Ho:ControKTreatment
TRANSFORMED MEAN CALCULATED INUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT SIG
1 CONTROL 1. 162 0.8302 • SDT-2 1. 219 6 . 870 -0 . 552
SDT-3 1.121 0.800 0.409SDT-4 0.629 0.350 5.231 *
5 SDT-5 1. 173 0 . 830 -0 . 105SDT-6 0.892 0.600 2.653 *SDT-9 1.016 0.720 i.439
innett table value - 2.43 (1 Tailed Value, P=0.05, df=24,6)
CHLORINE HYALELLA AZTECA 10 DAY SURVIVAIjTransform: ARC SINE (SQUARE ROOT(Y))
DUNNETTS TEST - TABLE 2 OF 2 Ho:Control<Treatment
NUM OF Minimum Sig Diff % of
' A-3
IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL
CONTROL 5SDT-2 5 0.215 25.8 -0.040
3 3DT-3 5 0.215 25.8 0.030SDT-4 5 0,215 25.8 0.480SDT-5 5 0.215 25-8 0.000
to SDT-6 5 - 0.215 25.8 0.2307 SDT-9 5 0.215 25.8 0.110
A-4
AR306867
AQUA SURVEY, INC, . SOLID PHASE LIVE COUNTS
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x———————————————————————————————————————RQUfl SURV€V. INC
A Q U A S U R V E Y INC.— — >f-
TEST ORGANISM ACCLIMATION & TRANSPORTATION
DATE : ??> _______ . QA/QC :_TEST JOB *:_4MI5__ CLIENT;IN-LAB [ i^] MOBILE [ ] SATELLITE [ J
TEST SPECIES: jOet (aTotal Number of organisms acclimated; |CCQ___AQUA SURVEY, INC Investigators: *X>eiO____________
A. ORGANISMS
1. ASI Culture/Holding [Tank/Receiving Log #]:_2. Procured From/Date Received: #QwjTfc, ?«tes£p»ft.Cfrf3. Age Information; -gi-degt s ftg en
B. RECEIVING WATER PARAMETERS (Non Cultured Organisms)1. Temperature:2. Salinity: ••//»•3 . Alkalinity: ... —
C. HOLDING [ V] CULTURE [ ] WATER PARAMETERS1. Temperature:_2. Salinity: __3. Alkalinity: __4. Water Source:
D. ACCLIMATION [Temperature - Water Type]1. Acclimation Chamber Volume (Liters):_2. Acclimation Water Type:3. Acclimation Water Temperature: - -_______4. Acclimation Water Salinity: .„.,,_______5. Acclimation Commencement - Date: ..__... Time;6. Change-Over Rate (ml/minute):_7, Culture Acclimation Ending -Date: ________ Time:8, ______ Acclimation Ending-Date: ________ Time:
TRANSFER CUSTODY & TRANSFER WATER PARAMETERS1. Culture Lab > Test Lab: -Date: l Time;2. Temperature ;3. Salinity:4. Alkalinity: ___r^— ..-_.5. Culture Lab Technician Initialsjrpj _____ [Transfer]6. Test Lab Technician Initials: , _____ [Receiving]
REMARKS;
. A-19Jl/I 499 Po/nf Breeze Road - Remington. New Jersey 08822 -Telephone (201) 788-871
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7L.3-
u>j m iwA-20
AR306883
A-21
-andard Chlorine Hyallela azteca 10 day growth«: amph Transform: NO TRANSFORMATION
i«piro Wilks test for normality
0.7000.967
: tical W (P » 0.05) (n - 35) - 0.934ritical w (P * 0.01) (n » 35) - 0.910
a .a PASS normality test at P^O.Ol level. Continue analysis.
tandard Chlorine Hyallela azteca 10 day growthile: amph Transform: NO TRANSFORMATION
a tletts test for homogeneity of variance
a ciliated B statistic 4.72a <le Chi-square value - 16.81 (alpha = 0.01)able Chi-sguare value « 12.59 (alpha • 0.05)rage df used in calculation «•»> df (avg n - 1) « 4.00
s .d for Chi-sguare table value => df (#groups-l) = 6
a a PASS homogeneity test at 0.01 level. Continue analysis.OTE: If groups have uneo ial replicate sizes the average replicate size is
used to calculate the B statistic (see above) .
rmLE: Standard Chlorine Hyallela azteca 10 day growth _I E: amph
NO TRANSFORMATION NUMBER OF GROUPS: 7
IDENTIFICATION REP VALUE TRANS VALUE
1 control 1 1.7000 1.70001 control 2 2.0000 2.00001 control 3 1.8000 1.80001 control 4 1.6000 1.60001 control 5 2.1000 2:10002 sdt-2 1 1.9000 1.90002 sdt-2 2 1.8000 1.80002 Sdt-2 3 2.0000 2.00002 sdt-2 4 2.0000 2.00002 Sdt-2 5 1.9000 1.90003 sdt-3 1 1.9000 1.90003 sdt-3 2 1.9000 1.90003 Sdt-3 3 2.1000 2.10003 Sdt-3 4 1.9000 1.90003 sdt-3 5 2.0000 2.00004 sdt-4 1 1.6000 1.6000
flR3Q6885
Sdt-4 2 1.8OOO Z.SOOOsdt-4 3 1.5000 1.5000Sdt-4 4 1.9000 -1.9000sdt-4 5 1.8000 1.8000Sdt-S 1 2.1000 ., 2.1000sdt-5 2 2.0000 2.0000sdt-5 3 2.2000 2.2000.
> Sdt-5 4 . 1.8000 1.8000i Sdt-5 5 1.8000 1.8000
sdt-6 1 1.7000 1.7000sdt-6 2 1.8000 1.8000Sdt-6 3 1.9000 .-.; 1*9000sdt-6 4 1.9000 1.9000sdt-6 5 2.2000 .2000sdt-9 1 1.7000 1.7000sdt-9 2 1.8000 1.8000Sdt-9 3 1.6000 1.6000sdt-9 4 1.7000 1.7000sdt-9 5 2.0000 2.0000
andard Chlorine Hyallela azteca 10 day growthle: amph Transform: NO TRANSFORMATION :
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 1 of 2
IDENTIFICATION N MIN MAX MEAN
control 5 1.600 2.100 1.840sdt-2 5 1.800 2.000 1.920sdt-3 5 1.900 2.100 1.960Sdt-4 5 1.500 1.900 1.720Sdt-5 5 1.800 2.200 1.980sdt-6 5 1.700 2.200 1.900sdt-9 5 1.600 2.000 1.760
andard Chlorine Hyallela azteca 10 day growthle: amph Transform: NO TRANSFORMATION
SUMMARY STATISTICS ON TRANSFORMED DATA TABLE 2 of 2
IDENTIFICATION ' VARIANCE SD SEM
control 0,043 0.207 0.093sdt-2 0.007 0.084 0.037Sdt-3 0.008 0.089 0.040sdt-4 0.027 0.164 0.073sdt-5 0.032 . 0.179 0.080sdt-6 0.035 0.187 0.084sdt-9 0.023 0.152 0.068
Chlorine Hyallela azteca 10 day growthiph Transform: NO TRANSFORMATION
ANOVA TABLE_______~___^^
A-23
DF SS MS
fc waenL-chin (Error)3 al
6
23
34
0.295
0.700
0.995
0.049
0.025
1.960
!critical F value 2.45 (0.05,6,28)inc© F < Critical F FAIL TO REJECT Ho:All groups equal
: ndard Chlorine Hyallela azteca 10 day growthLuug: amph Transform: NO TRANSFORMATION
DUNNETTS TEST - TABLE 1 OF 2 Ho:Control<Treatment
UP IDENTIFICATION
controlsdt-2sdt-3sdt-4sdt-5sdt-6sdt-9
TRANSFORMEDMEAN
1.8401.9201.9601.7201.9801.9001.760
MEAN CALCULATED INORIGINAL UNITS .
1.8401.9201.9601.7201.9801.9001.760
T STAT
-0.800-1.2001.200-1.400-0.6000.800
SIG
tabl§ value » 2.43 (1 Tailed Value, P=0.05, df=24,6)
:andard chlorine Hyallela azteca 10 day growthlie: amph Transform: NO TRANSFORMATION
DUNNETTS TEST - TABLE 2 OF 2 Ho:Control<Treatment
NUM OF Minimxua Sig DiffR UP IDENTIFICATION REPS (IN ORIG. UNITS)
1 control• sdt-2sdt-3
4 sdt-45 sdt-5
sdt-6sdt-9
5555555
0.2430.2430.2430.2430.2430.243
% Of DIFFERENCECONTROL FROM CONTROL
13.213.213.213.213.213.2
-0.080-0.1200.120-0.140-0.0600.080
A-24 aR3Q6887
SAMPLE ID: SRT H. azteca 9/10/91 SDS-TThrs,
- BINOMIALNUMBER NUMBER PERCENT PROBABILITY• EXPOSED AFFECTED AFFECTED . (%)
40.0000 20 - 20 100.0000000 0.000095420.0000 20 19 95.0000000 0.002002710.0000 20 11 55.0000000 41,19015005.0000 20 0 0.0000000 0.00009542.5000 20 4 20.0000000 0.5908966
RESULTS
95 PERCENT[ETHOD SPAN G EC50 CONFIDENCE LIMITS
J.NOMIAL _ . _ 9.552655 5.000000 - 20.000000
7ING AVERAGE 4 0.097538 j 9.475162) 6.964456 - 12.741630
POBIT 1.4879893BIT (...........,............NOT VALID......................
INTERPRETATION OF STATISTICS
The order of preference of results is:BEST: Probit MethodBETTER: Moving Average " ,_-- -GOOD: BinomialGOOD: Graphic Interpolation (Graph drawn by hand)
assuming all are statistically valid.
To Determine if a method has given valid results:GRAPHICAL: Always validBINOMIAL: The program will tell you if this is not valid.MOVING AVERAGE: The number of spans needs to be no less than one
lower than the number of concentrations tested(excluding control).
PROBIT: The program will tell you if this is not valid
.:-;T;sT... / AR306888
SAMPLE ID: SRT H. aztmca 9/10/91 SDS 96 hrs.
BINOMIAL.NUMBER NUMBER PERCENT PROBABILITY
SCENTRATION EXPOSED AFFECTED AFFECTED . (%)40.0000 20 . 20 100.0000000 0.0000954-20.0000 20 19 95.0000000 0..002002710.0000 . 20 17 85.0000000 0.12834145.0000 20 2 10.0000000 0.02012252.5000 20 12 60.0000100 25.1722300
RESULTS
95 PERCENTffiTHOD SPAN G EC50 CONFIDENCE LIMITS
J.UNOMIAL 4.811202 0.000000 - 10.000000
J7ING AVERAGE 2 0.103108 7.434621 5.711770 - 9.039545
?ROBIT 3.668207? OBIT (........................NOT VALID...............I.....,.)
INTERPRETATION OF STATISTICS
order of preference of results is:BEST: Probit MethodBETTER: Moving AverageGOOD: BinomialGOOD: Graphic Interpolation (Graph drawn by hand)
assuming all are statistically valid.To D©termine if a method has given valid results:GRAPHICAL: Always validBINOMIAL: The program will tell you if this is not valid.MOVING AVERAGE: The number of spans needs to be no less than one
lower than the number of concentrations tested(excluding control).
PROBIT: The program will tell you if this is not valid
A-26flR306889
AQUA SURVEY, INCFRESHWATER ORGANISMS
96 HOUR REFERENCE TOXICANT
TODCICANT: SODTUH DOPECYL SULFATE LOT *t______- TEST VOUfC:
START DATE: _____7'/^-V/_______________ DILUTION WATER:
START TINE: fc-j*^^__________________ TEST TEMPERATURE:
<NUO » NOTHING UNUSUAL OBSERVED)NOTES AND OBSERVATIONS:
__________________;_______________, SRT-LC.fU
A-27 fiR306890
AQUA SURVEY, IHC
TOSHUATBl OftOAIIISHS
96 HOA JIEFERB4CE TOXICANT
u_£SPCCIESi /T &Z3&+G_________________ TEST TOUJH6:.
TOXICAMTsSGOUJN OCSECn.-mLFATl LOT *S DILUTION UATERs
START DATEs -/&'*>/ _______________ TEST TEHPBUTUaEs
START
SAHPU ID
DISSOLVED OXTGEX
00 48 72 96
TSVERATURE<DECKEES CELCIUS3
X 24 48 72 96
p«00 24 48 72 96
COWJUCTWITY(unhos/cm)
00 24 48 72 96
CONTtOL A ns.COMTKOL I 64? 1*0
t.Sfl 3-7 175*-
f) ftc 7,7 **
3flT £5 73V
B in--Vj j y 7,4 75V
8 a -f
U /$&/_
INITIALS - c
OA/OC
. ftR30689lA-28
Aqua-Survey Stock and Treatments Preparation Sheet**** ALL UNITS ARE IN PPM UNLESS OTHERWISE SPECIFIED ****
Job No. :QA/QC
Date : 9/10/91
TOXICANT :SLS
Species : H . azteca
Location :ASI Flemington,NJ
SOURCE/LOT No.: Aldrich 70%
Enter ratio of stock solution to highest test concentration in 14A:5.00 Enter Stock Solution Volume: 1000.00 ml.
Enter concentrations in PPM, highest to lowest, in column .-A below:40,0020.0010.005.002.50 _0.00
Enter total volume of each container in Milliliters in A-25:500.00
Enter number ofreplicates per treatment in A-27:2.00
iter excess solution for each treatment in A-29:0.00 . Total ml= 1000.00
First, prepare STOCK SOLUTION by adding0.2000 grams [Note:Stock cone.,mg./ml. 0.20of stock chemical to a 1000.00 ml. volumetric flask.
Mix, then pour to mixing chambers as follows:Cone., PPM ml. Stock Solfn. ml. Dilution Water Total Vol
0.00 0.00 1000.00 1000.00
0.00 0.00 1000.00 1000.00
2.50 12.50 987.50 1000.00
5.00, 25.00 975.00 1000.00
10.00 50.00 950.00 1000.00
20.00 100.00 900.00 1000.00
40.00 200.00 800.00 1000.00
Totals: 387.50 6612.50 7000.00
By: Steven Grasso
BR306892
SURV6V, INC
A Q U A S 0 S V E Y INC.
TEST ORGANISM ACCLIMATION & TRANSPORTATION
DATS; uf _______ . 'QA/QC:TEST JOB f s •SffrT CL22KT:IN-LAB [ t*n MOBILE [ ] SATELLITE [ ]
TEST SPECIESs UuA ltd* A^+tttr _______________Total Number of organisms acclimated :AQUA SURVEY, INC Investigators:.
A. ORGANISMS1. ASI Culture/Holding [Tank/Receiving Log f ] :a2. Procured From/Date Received:/3, Age Information: /-;
B* RE'STyiMG WATER PARAMETERS (Non Cultured Organisms)1. Temperature:2. Salinity:3, Alkalinity: ^ /
C. HOLDING £ vj CULTPSE [ ] WATER PARAMETERS
2. Salinity:3. Alkalinity: '4. Water Source
D. ACCLIMATION [Tas perature - Watter Type]1. Acclamation Cha iaer Volume (Liters):2. Acclimation Water Jroe: ,3. Acclimation Water Temte ra rfe:4* Acclamation Water Salinifcf* ,5. Acclimation Commencement -"NJate: Time:6. Change-Over Rate (ml /minute) 27* Culture Acclimation Ending-DateV8. Acclimation Ending-Date: v
E. TRANSFER CUSTODY & TRANSFER WATER PARAMETERS1. Culture Lab > Tj&st Lab: -Dat@:_£//o/2 . Temperature : r5? ri "Cl / '3. Salinity: AJ/A- .4. Alkalinity: c?**n * f jf. < ^ — S. Culture Lab Technicimn Initials :Cfcjr56. Test Lab Technician Initials: sjf
REMARKS:
Time:Time:
Time : /£3f%}?S
[Transfer][Receiving]
•
A-30
•
O/1 499 Point Bteeze Road - Remington. New Jersey 08822 • Telephone (201) 788-87
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11.
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Percent Moisture = (Utt saiele & dish) - fPrv saaole & dish) t 100(Met saipie & dish) • dish
Hygroscopic Moisture Correction Factor = oven dried / air dried
A-31
HygroscopicItoisture
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AR30690I
Date: 09/26/91 Page:
ta te JS/24/91 Total # of Samples: 5iid Hkidard Chlorine Control SDT2,3,4,5 K Contant: 0.01348 Number of Entries: 7i I ^ Pc Correction:
• ^**Die #: Control-lockTime
>:18?:21
o :3109:46"3:16l:26?:16
ElapseTime
CT)
2.05.015.030.060.0250.01440.0
4,000 Temperature: 21 .000 Specific Gravity: 2.65Cf SDT . f
Grams Used: 50.00 _m: 0.98000 Sample #: 2 Grams Used: 50.00 m: 1.00000
HydrometerReadingRh
23.0017.0015.0013.0011.00
STDpie #: 3
-lockTime
09:3409:37~9:473:02.0:3201:42
ElapseTime
CT)
2.05.015.030.060.0250.0
09: 1440.0
8.006.00
EffectiveDepth
CD
12.5013.5013.8014.2014.50
' 15.0015.30
PercentSuspend
CX)
38.77551026.53061222.44898018.36734714.2857148.1632654.081633
GrainSize (mm)
CGS nn.)
0.03370000.02214990.01292960.00927410.00662670.00330190.0013895
ClockTime
09:3009:3309:4309:5810:2801:3809:28
ElapseTime
CT)
2.05.015.030.060.0250.01440.0
HydrometerReading
I H6.006.006.006.006.005.005.00
EffectiveDepth
CD
15.3015.3015.3015.3015,3015.5015,50
PercentSuspend
CX)
4.0000004.0000004.0000004.0000004.0000002.0000002.000000
GrainSize (ran)
CGS mm.)
0.03728380.02358040.01361410.00962660.00680710.00335650.0013985
f SDT fGrams Used: 50.00 m: 0.98000 Sample #: 4 Grams Used: ' 50.00 tn: 0.98000
HydrometerReading
Rh
42.0036.0027.0026.0021.0016.0012.00
^ B~SDTnp «W 5
"lockTime
?:42?:45u9:5510:10"5:40l:50?:40
ElapseTime
CT)
2.05,015.030.060.0250.01440,0
EffectiveDepth
CD
9.4010.4011.9012.0012.9013.7014.30
PercentSuspend
C%)
77.55102065.30612246.93877644.89795934.69387824.48979616.326531
GrainSize (mm)
CGS am.)
0.02922390.01944110.01200650.00852550.00625040.00315560.0013433
ClockTime
09:3809:4109:5110:0610:3601:4609:36
ElapseTime
CT)
2.05.015.030.060.0250.01440.0
HydrometerReading
I hl30.0025.0019.0016.0014.0010.009.00
EffectiveDepth
CD
11.4012.2013.2013.7014.0014.7014.80
PercentSuspend
CX)
53.06122442.85714330.61224524.48979620.40816312.24489810.204082
GrainSize (mm)
CGS on.)
0.03218310.02105640.01264540/00910940.00651150.00326870.0013666
f . fGrams Used: 50.00 m: 0.97000 Sample #: 6 Grams Used: 0.00 m: 0.00000
HydrometerReadingRh
51.0047.0041.0036.0032.0025.0020.00
EffectiveDepth
CD
7.908.609.6010.4011.1012.2013.00
PercentSuspend
CX)
96.90721688.65979476.28866065.97938157.73195943.29896932.989691
GrainSize (mm)
CGS mm.)
0,02679100.0176789
. 0.01078400,00793680.00579800.00297780.0012808
ClockTime
ElapseTime
CT)
HydrometerReading
1 h!
EffectiveDepth
CD
PercentSuspend
CX)
GrainSize (mm)
CGS mm.)
A-39
AR3G6902
Dates 09/26/91 Page:
>ate: 09/24/91 Total # of»ite; Standard Chlorine K Content: 0.01348 Munber of Entries
persant Correctlom 4.000 Teaperatures 21.000 Specific Gravity: 2.f . ... f
#: SHT-6 ' Graaw Ueeds 50.00 •: 0.96000 Saiaple #: STD-9 Gramc Us*d: 50*°° *"* °-97ao°clockTime
9:469:49
.9:5910s1410:441:549:44
glepceTine
(T)
2.05.0
15.030.060.0250.01440.0
HydrometerReedingRh
49.0047.0041.0037.0033.0026.0020.00
EffectiveDepth
CD
8.308.609.6010.2010.9012.0013.00
PercentSuspend
93.75000089.58333377.08333368.75000060.41666745.83333333.333333
GrainSize (an)
<GS wn.)
0.02746080.01767890.0107S400.00786010.00574550.00295330.001 2808
ClockTime
09:5009:5310:0310:1810:4801:5809:43
ElapseTine
2.05.0
15.030.060.0250.01440.0
HydrometerReading
I h|52.0047.0039.0034.0027.0020.0015.00
EffectiveDepth
CD
7.808.609.9010.7011.9013.0013.80
PercentSuspend
CX)
98.96907288.65979472.16494861.85567047.42268032.98969122.680412
GrainSize (urn)
(GS m.)
0.02662090.01767890.01095120.00805050.00600330.00307390.0013196
A-40
AR306903
Climta « a « A - <?_____ Job t:
Saiple 1
SDT-2-
SQ7-S
Pan HeightPan Ueigftt& ««t Sed.
1
1./. />f ?1 /fcyj ZaWfT
1.1*1 ft-1.
Pan Height\L Air Dry
PercentMoisture
57.54i 5 "~) jj
35.3116
5?. 9 3
Pan Mtighti liven Dry
HygroscopicHoisture
t -
Percent Moisture = (Met saanlfr %. dish) - Cflrv saiole '* dish) $ 100(fet saipie & dish) - dish
tt Hygroscopic Moisture Correction Factor = oven dried I sir dried
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A-43 AR306906
APPENDIX LCHEMICAL CONCENTRATIONS IN VEGETATION - MEADOW VOLE
flR306907
Table L-1
Estimation of Exposure to the Meadow Vole through Vegetation Ingestion
Chemical
Benzene.Chlorobenzene1 ,2-Dichlorobenzene1 ,3-Dichiorobenzene1 ,4- DichlorobenzeneEthylbenzeneHexachlorobenzeneMetachloronitrobenzeneNitrobenzenePCBAroclor-1016Aroclor-1221Arocfor-1232Aroclor-1242Aroclor-1248 -Aroclor-1254Aroclor- 1 260Pentachlorobenzene1 ,2,3,4-Tetrachiorobenzene1 ,2,4,5-TetrachlorobenzeneToluene1 ,2,3-Trichlorobenzene1 ,2,4-Trichlorobenzene1 ,3,5-Trichlorobenzene
: Sediment ;{•} Concentration
; ; tig/kg):p-< ',,;'>:£•;;. "'I'.. *Jpf>er '
Mean 9$%
3.00E+009.50E+011.76E+021.09E+022.68E+034.60E+011.26E-011.58E-014.92E-01
NANANANANANA
1.95E-012.70E+014.70E+011.00E+014.20E+013.60E+014.00E+003.80E-01
6.00E+002.47E+024.74E+022.93E+027.24E+031.25E+022.56E-012.57E-018.18E-01
NANANANANANA
7.31 E-016.80E+011.24E+022.30E+011.15E+029.50E+018.00E+005.63E-Q1
LogKow
2.172.843.483.513.463.216.182.441.86
NANANANANANA6.805.074.724.702.714.054.224.22
Bv
2.16E+008.84E-013.77E-013.62E-013.87E-015.40E-011.04E-021.51E+003.26E+00
NANANANANANA
4.55E-034.55E-027.24E-027.44E-021.05E+001.77E-011.41 E-011.41 E-01
VegetationConcentration(mg/kg)
UpperMean 95%
1.51E+019.99E+013.39E+011.16E+011.49E+035.94E+001.41E-032.68E-013.11E+00
NANANANANANA
2.55E-045.91 E-011.59E+005.21 E-011.37E+014.06E-i-002.82E+006.14E-02
2.59E+011.54E+026.37E+022.79E+022.63E+031.62E+011.89E-033.60E-013.26E+.00
NANANANANANA
6.09E-041.09E+002.90E+008.18E-013.26E+016.89E+005.49E+008.58E-02
AR306908
![Integrin function and misfunction INTEGRINS...cyclo [cis-DKP1-RGD] MeOH H2-Pd/C (L-Asp and L-Ser) N H NHCbz O COO N H NH2 O COO O H O N N O HN Bn HATU 3eq NH HOAt 3 eq collidine 6](https://static.fdocuments.us/doc/165x107/60ce3d5580629875053c6c70/integrin-function-and-misfunction-cyclo-cis-dkp1-rgd-meoh-h2-pdc-l-asp-and.jpg)
