Fsc result

STEM CELLS AND REGENARATIVE MEDICINE

O.L. Kukharchuk, V.V. Radchenko,

A.B. Padma Priya, A.O. Kukharchuk

EmProCell Clinical Research Pvt. Ltd., Mumbai

SPEAKER:PROFESSOR

KUKHARCHUK OLEKSANDR

Currently in EmProCell cryobank we have fetal stem cells of the brain, spinal cord, heart, lungs, liver, kidneys, pancreas and adrenal, muscles and bones, skin and placenta. We are open for collaboration in the field of Regenerative Medicine. Contact us: [email protected], [email protected]

mailto:[email protected],[email protected]

FETAL PROGENITOR CELLS ARE THE CELLS DERIVED FROM THE TISSUES OF ABORTUS FETUS

• The following types of cells can be derived from abortive material of different gestation terms (from 6 to 20 weeks):

• - liver progenitor cells• - neural progenitor cells• - gastric and intestinal

progenitor cells• - lung progenitor cells• - kidney progenitor cells• - skin progenitor cells• - bone tissue progenitor

cells• - pancreatic progenitor

cells

Fetal chondrocytes has high potential to

multiplication, they can be isolated in sufficient

quantity for transplantation or for obtaining specialized fetal growth factors

Fetal chondrocytes

FETAL PROGENITOR CELLS FROM HEART TISSUE

From fetal heart tissue have been isolated human fetal cardiomyocytes, cardiac human fibroblasts, adventitial fibroblasts, human microvascular endothelialcells, endothelial progenitor cells, mesenchymal stem cells, and vascular smooth muscle cells.Transplantation of these cells is effective for treatment of myocardial infarct and dilatation cardiomyopathy.

HAEMOPOIETIC PROGENITOR CELLS OF FETAL LIVER WITH PHENOTYPE CD133+

Progenitor hematopoietic cells of fetal liver capable to angio-myogeneic differentiation, stimulates blood cells formation and formation of new vessels, induces central immunological tolerance and enables regeneration of tissues practically all damaged organs

Ischemic muscle tissue: the area of myosymplast with

myoni

Three months after cells transplantation

HEMATOPOIETIC PROGENITOR CELLS OF FETAL LIVER WITH PHENOTYPE CD133+

Human CD133 fetal liver progenitor cells promote the healing of diabetic ischemic ulcers by paracrine stimulation of angiogenesis.Accelerated wound closure in the presence ofCD133 cells (A, middle) is associated with higher temporary wound capillarization at day 7 (B). Capillary density declines tolevels of wounds treated with CD133 cells or collagen gel alone by day 14 (C)

Tissue of fetal kidney consists all cellular elements in developmental stages, which has potential to replace damaged (injured) renal structures in adults.From 12-13 and till 18-20 weeks of human gestation: (a–b) localization of SIX2 to the MM, predominantly to the CM. (c–d) shaped bodies and renal stroma.(e–f) nephrogenic zone and newly forming tubules. (g–h) nephrogenic cortex, parietal epithelium of fetal glomeruli.(i–j) some differentiated tubules.

FETAL KIDNEY PROGENITOR CELLS

Neural fetal progenitor cells consists from precursors of primary neuroglia, dopaminergic neurons and oligodendrocytes, that lies on the grounds of therapeutical effects of the fetal neural cells transplantation in neurodegenerative diseases

FETAL NEURAL PROGENITOR CELLS

FETAL PANCREATIC PROGENITOR CELLS

Fetal pancreas consist cells – precursors of Langerhans islets cells, that could be the basis for treatment in diabetes mellitus 1 type

FETAL PROGENITOR CELLS TRANSPLANTATION

Bright example of fetal progenitor cells induced reparative regeneration is polytrauma.

In these conditions, likewise after any complicated operation, body of patient mobilizes all stem cells resources to restore the function of the damaged organs and tissues.

If stem resources is insufficient then the patient dies.

Regenerative medicine contributes chance of survival in such type of patients.

FETAL PROGENITOR CELLS IN BURNS III-A STAGE TREATMENT



TRANSPLANTATION OF FETAL PROGENITOR CELLS IN POSTOPERATIVE PERIOD TO PANCREONECROSIS PATIENTS

Sewing of umbilical cord

Ready implant

Insertion of implant on the zone of pancreatic necrosis

De-freezing umbilical cord




Removal of cord tissue implant following 3 days after operation




Fetal progenitor cells

Introduction of fetal progenitor cells stem cells in the subclavian

veinCurrently in EmProCell cryobank we have fetal stem cells of the brain, spinal cord, heart, lungs, liver, kidneys, pancreas and adrenal, muscles and bones, skin and placenta. We are open for collaboration in the field of Regenerative Medicine. Contact us: [email protected], [email protected]

Mortality rate reduced to 9 times. In course of 3 years after treatment formation of cysts in pancreas were not

observed


CHRONIC LEGS ISCHEMIA

In clinic 65 patients were examined with chronic legs ischemia. Age of patients: from 55 to 78 years. Patients had last stage of disease (alternative treatment – leg amputation)

CHRONIC LEGS ISCHEMIA• Type of cells

suspension: hematopoietic cells of fetal liver.

• Gestation term: 12 weeks.

• Dose: 35,0 x 10^6/ml. • Phenotype: CD34^+

CD133^+ CD38^- CD45RA^low CD71^low CD7HLA-DR^low.

• Amount of transplantation: 1

• Follow-up period: 16 months

CHRONIC LEGS ISCHEMIA

Surgical treatment: local injection of Fetal progenitor cells along the sclerotic arteries

CHRONIC LEGS ISCHEMIA: CASE REPORT

BEFORE CELL TRANSPLANTATION

AFTER CELL TRANSPLANTATION

Level of microcirculation (unit) Before treatment - 4.28 ±0.34 1 month after EPC–transplantation - 5.24±0.27 (р<0.05) 3 month after EPC–transplantation - 6.32±0.41 (р<0.05) 6 month after EPC–transplantation - 7.57±0.62 (р<0.01)Reserve of capillarity blood stream (%) Before treatment - 89.32±12.5 1 month after EPC–transplantation - 112.34±10.9 (р<0.01) 3 month after EPC–transplantation - 137.83±9.2 (р<0.05) 6 month after EPC–transplantation - 150.31±10.5 (р<0.05)Dilatation of precapillary sphincters Before treatment - ( - ) 1 month after EPC–transplantation - ( + ) 3 month after EPC–transplantation - ( + ) 6 month after EPC–transplantation - ( + )Distance of painless walk Before treatment – 50 m 1 month after EPC–transplantation - 300 m 3 month after EPC–transplantation - 450 m 6 month after EPC–transplantation - 500-1500 m

RESULTS OF ENTIRE CELL-TREATMENT GROUP

PATIENTS ESTIMATION ON EFFICACY OF FETAL PROGENITOR CELLS TRANSPLANTATION AT

THE END OF IVESTIGATION:“SIGNIFICANT IMPROVEMENT” – 90%

“IMPROVEMENT” – 10%“NON SIGNIFICANT IMPROVEMENT” – 0%

“WITHOUT CHANGE” – 0%

LIVER CIRRHOTIC PATIENTS

In clinic 43 patients were examined with alcoholic liver cirrhosis:

• Age of patients: from 35 to 55 years.

• Disease span - 7,100,25 years.

• Patients had last stage of disease with higher level of liver injury (alternative treatment – liver transplantation)


• Type of cells suspension: hematopoietic cells and hepatoblasts of fetal liver

• Gestation term: 6-8 weeks

• Dose: 15,0 x 10^6/ml. • Phenotype: CD34^+

CD45RA^low CD71^low CD7HLA-DR^low Albumine^+

• Amount of transplantation: 1

• Follow-up period: 14 months


FPC- transplantati

on


FPC- transplanta

tion

PATIENTS ESTIMATION ON EFFICACY OF FETAL PROGENITOR CELLS RANSPLANTATION AT THE END OF

INVESTIGATION:

“SIGNIFICANT IMPROVEMENT” – 95%

“IMPROVEMENT” – 5%

“NON SIGNIFICANT IMPROVEMENT” – 0%

“WITHOUT CHANGE” – 0%

DIABETES MELLITUS TYPE 2

• FPC-transplantation significantly decrease blood levels of glucose and Hb-Glu in patients with type 2 of diabetes mellitus

FPC- transplantati

on

DIABETES MELLITUS TYPE 2

FPC-transplantation significantly decrease blood levels of insulin and C-peptide

FPC- transplantatio

n

SEXUAL DYSFUNCTION

• Treatment: intravenous and subcutaneous human FPC administration.

• Type of human cells suspension: hematopoietic cells of fetal liver and progenitor cell, all type.

• Gestation term: 6-7 weeks. • Dose: 55,0 x 10^6/ml. • Phenotype: CD34^+ CD133^+

CD38^- CD45RA^low CD71^low CD7HLA-DR^low.

• Amount of transplantation: 1• Follow-up period: 26 months

CHARACTERISTICS OF SEXUAL FUNCTION BEFORE FETAL PROGENITOR CELLS TRANSPLANTATION

Before start of tests

2 of 4 men have imperfect sexual function.

2 of them has climax. Two patient has subnormal libido, its absence – two other patient.

All 6 women has

climax with absence of libido

Patient Patient sexsex ageage ObservatiObservation time on time ((monthsmonths))

Sexual Sexual function function

Libido Libido

G1G1 manman 5858 1414 Not Not infringedinfringed

Subnormal Subnormal

G2G2 manman 6565 1212 Climax Climax Absent Absent

G3G3 manman 6161 1818 Not Not infringedinfringed

SubnormalSubnormal

G4G4 manman 6363 1515 ClimaxClimax AbsentAbsent

G5G5 womanwoman 5959 1818 ClimaxClimax SubnormalSubnormal

G6G6 womanwoman 6060 1414 ClimaxClimax AbsentAbsent





CHARACTERISTICS OF SEXUAL FUNCTION AFTER FETAL PROGENITOR CELLS TRANSPLANTATION

Six months after transplantation the potency of all men was restored on a back of sharp rise of libido.

3 women have

restored menstrual cycle. Appearance of libido – all of them.

Patient Patient sexsex ageage Observation Observation time time ((monthsmonths))

Sexual function Sexual function Libido Libido

G1G1 manman 5858 1414 Not infringedNot infringed Elevation Elevation

G2G2 manman 6565 1212 Potency is Potency is renewed renewed

ElevationElevation

G3G3 manman 6161 1818 Not infringedNot infringed ElevationElevation

G4G4 manman 6363 1515 Potency is Potency is renewed renewed

ElevationElevation

G5G5 womanwoman 5959 1818 Appearance of Appearance of menstrual flux menstrual flux

ElevationElevation

G6G6 womanwoman 6060 1414 Without changesWithout changes Appearance Appearance

G7G7 womanwoman 5858 1212 Appearance of Appearance of menstrual fluxmenstrual flux

AppearanceAppearance

G8G8 womanwoman 6868 1616 Appearance of Appearance of menstrual fluxmenstrual flux


G9G9 womanwoman 6767 1616 Without changes Without changes


G10G10 womanwoman 6565 1717 Without changesWithout changes AppearanceAppearance

THE CLINICAL EFFECTIVENESS OF THE FPC-TRANSPLANTATION AT DIFFERENT FORMS OF MALE INFERTILITY

We performed complex investigation of 29 infertile men; with secretory infertility – 19 patients; with excretory – 10 patients. The program of investigation included sperm's analysis and explanation of the sex hormones level in blood.

The control investigation were conducted over 3 month after the FPC-transplantation.

THE CLINICAL EFFECTIVENESS OF THE FPC-TRANSPLANTATION IN SECRETORY FORM OF MALE INFERTILITY

Sperms volume, ml Before transplantation (n=19) - 3,73±0,50 After transplantation (n=19) - 3,93±0,38Concentration of spermatozoids (bill/ml) Before transplantation (n=19) - 4,81±0,96 After transplantation (n=19) - 11,32±1,43Amount of spermatozoids (bill) Before transplantation (n=19) - 14,89±0,82 After transplantation (n=19) - 43,89±4,82

THE CLINICAL EFFECTIVENESS OF THE FPC-TRANSPLANTATION IN EXCRETORY FORM OF MALE INFERTILITYSperms volume, ml Before transplantation (n=10) - 3,86±0,17 After transplantation (n=10) - 4,30±0,11Concentration of

spermatozoids (bill/ml)

Before transplantation (n=10) - 49,75±4,03 After transplantation (n=10) - 53,62±5,26Amount of

spermatozoids (bill) Before transplantation (n=10) - 141,00±8,40 After transplantation (n=10) - 227,20±17,1

Motility of spermatozoids “a” (%)

Before transplantation (n=10) - 6,60±044 After transplantation (n=10) - 13,25±1,08Motility of

spermatozoids “b” (%) Before transplantation (n=10) - 11,67±1,03 After transplantation (n=10) - 16,75±1,19Motility of

spermatozoids “a + b” (%)

Before transplantation (n=10) - 17,17±1,42 After transplantation (n=10) - 30,00±1,75

CONCLUSIONS

• We established the statistically true increasing of concentration and quantity of spermatozoids in secretory infertility (67%); and statistically true increasing of motility of spermatozoids in excretory infertility men (70%).

• After transplantation 40% treatments men had positive reproductive result.

THANKS FOR YOUR ATTENTION !

More detailed information about the clinical application of fetal progenitor

cells mentioned in the information brochure

Fsc result

Healthcare

Transcript of Fsc result