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    Fruits and vegetables are important components of our diet. We take

    fruits in raw state or in the form of juices, while vegetables are

    taken in the cooked form or sometimes as juices. Fruits and vegetables

    provide us carbohydrates, proteins and various other important organic

    compounds. The  juices are rich sources of minerals, vitamins and many

    micronutrients (calcium, iron etc.) essential for us. For example,

    iron deficiency in humans causes anaemia and anaemic persons are

    advised to take leafy vegetables e.g. spinach and apples which fills

    up the iron deficiency. Vitamin-C deficiency causes scurvy which

    results in bleeding of gums. This disease can be cured by taking

    plenty of Vitamin-C rich fruits like lemon, orange, guava, amla, etc.

    Some fruits like papaya and vegetables like carrot are rich in vitamin

    A which is essential for eyes to prevent night blindness. In addition,

    the fruits and vegetables , particularly their juices provide instant

    energy to children, weak or old persons and patients too, as they can

    be digested easily. This clearly shows that the fruits and vegetables

    play a vital role in maintaining our good health.

    Fruits and vegetables are seasonal and grown in different parts of our

    country. They need to be stored for longer period and transported to

    different places. It makes necessary for us to find out whether there

    is any damage to the usefulcontents of the fruits andvegetable 

    during the storage and transportation or due to some preservatives or

    other factors and what kind of nutrients, minerals, etc. are present

    in their juices. With this idea in mind, the project has been

    undertaken.

    AIM - To analyse some fruit and vegetable juices for the contents

    present in them.

    APPARATUS - Test tubes, burner, litmus paper, beaker, tripod stand,

    conical flasks, burette, pipette.

    CHEMICALS REQUIRED -

    1. Fehling's solution A

    2. Fehling's solution B

    3. Tollen's reagent (Ammoniacal AgNO3)

    http://www.123helpme.com/search.asp?text=juiceshttp://www.123helpme.com/search.asp?text=juiceshttp://www.123helpme.com/search.asp?text=contentshttp://www.123helpme.com/search.asp?text=contentshttp://www.123helpme.com/search.asp?text=vegetablehttp://www.123helpme.com/search.asp?text=contentshttp://www.123helpme.com/search.asp?text=vegetablehttp://www.123helpme.com/search.asp?text=juices

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    Identification Tests

    A. Fehling's solution Test (given only by reducing carbohydrates)

    Fehling's solution is an alkaline solution of copper sulphate

    containing sodium potassium tartrate (Rochelle salt) as the complexing

    agent.

    Being strong reducing agents, aldehydes reduce Fehling solution to red

    ppt. of cuprous oxide.

    [IMAGE]RCHO + 2Cu2+ + [IMAGE] [IMAGE] + Cu2O + 3H2O

    Aldehyde

    [IMAGE]

    B. Tollen's Reagent Test (given only by reducing sugars)

    Tollen's reagent is an ammoniacal solution of silver nitrate and is

    prepared by adding NH4OH solution to AgNO3 solution till the ppts. Of

    Ag2O first formed just redissolve.

    When an aldehyde is heated with Tollen's reagent, the latter is

    reduced to metallic silver which deposits on the walls of the test

    tube.

    [IMAGE]2AgNO3 + 2NH4OH Ag2O + 2NH4NO3 + H2O

    Grey ppt.

    [IMAGE]Ag2O + 4NH4OH 2[Ag(NH3)2]+[IMAGE] + 3H2O

    Tollen's Reagent

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    C. Benedict's Test (given only by reducing carbohydrates)

    Benedict's solution is alkaline solution containing citrate ions as

    complexing agent.

    Aldehydes, on warming with this solution, give brick red precipitates.

    Red ppt.

    [IMAGE]RCHO + Cu2+ Cu2O + Oxidation Products

    D. Iodine solution test (given only by starch)

    Presence of starch is tested with iodine which gives blue-black colour

    with starch.

    2. FATS AND OILS

    Fats, oils and their derivatives found in living system belong to the

    class of substance called lipids. These are sparingly soluble in water

    but highly soluble in organic solvents. Fats and oils are glycerol

    esters of different fatty acids. For example, glyceride of unsaturated

    fatty acids like oleic acid [C17H33COOH] are called unsaturated fats

    and similarly glyceride of saturated fatty acids like Stearic acid [C17H35COOH]

    are called saturated fats. Unsaturated fats can be saturated by

    addition of hydrogen. This process is called hydrogenation. These are

    excellent sources of energy and fats on oxidation provide about twice

    as much as energy as that produced by carbohydrates . Important

    sources of fats & carbohydrates are peanuts, coconut, soyabean,

    butter, cottonseeds mustard, etc.

    Identification Tests

    A. Fats and oils have generally low melting points. If a fat is shakenwith either ethanol or chloroform in a dry test tube, it dissolves

    giving a clear solution.

    B. To the given oil or fat sample , add few crystals of potassium

    hydrogen sulphate (KHSO4). After heating, a pungent irritating odour

    due to the formation of acrolein confirms the presence of an oil or a

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    fat.

    The oil or fat on heating undergoes hydrolysis to give glycerol and

    fatty acids. The glycerol thus produced on strong heating undergoes

    dehydration to form acrolein which has irritating smell.

    [IMAGE]

    [IMAGE] Oil or fat Glycerol + Fatty Acids

    CH2OH CH2

    [IMAGE]

    KHSO4

    [IMAGE]

    [IMAGE]

    [IMAGE][IMAGE][IMAGE][IMAGE] CH2OH CH + 2H2O

    CH2OH CHO

    3. PROTEINS

    Proteins are essential organic compounds for any living system to

    build and repair our body. Proteins are main constituents of muscles,blood, skin, hair and other body parts. Besides carbon, hydrogen and

    oxygen, other elements such as nitrogen and sulphur are in proteins.

    Proteins are made up of chains of amino acids and are very essential

    components of our food. Beans, pulses, fish, meat, milk, cheese, etc.

    are rich sources of proteins.

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    Identification Tests

    A. Biuret Test

    In this test, proteins when heated with strong alkaline copper

    sulphate (blue) give a violet or pink colour.

    This test is meant for testing the compounds having having a CONH2

    group called peptide linkage. As the proteins contain long chains of

    peptide linkages, they give positive biuret test by forming complex

    with Cu2+ ions.

    B. Xanthoproteic test

    To the protein sample, add few drops of conc. HNO3. A yellowprecipitate confirms the presence of proteins.

    This test is based upon the formation of yellow coloured aromatic

    nitro compounds which are produced by the action of nitric acid on the

    benzene rings present in some of the [IMAGE]- amino acids (tyrosine,

    phenylamine, etc.) constituting the protein.

    4. UNSATURATION

    The unsaturation in the organic compounds is due to the presence of

    double or triple bonds in its constituent compounds.

    Identification test

    Sulphuric acid Test

    In this test, the compound is taken in a clear test tube and conc. H2SO4

    is added carefully dropwise. The contents are carefully shaken. The

    unsaturated compound dissolves in conc H2SO4 due to the formation ofalkyl hydrogen sulphate (double bonds break to form single bonds).

    [IMAGE]

    [IMAGE]

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    [IMAGE]

    [IMAGE]

    [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE] C C + H2SO4 C

    C

    OSO3H

    5. HYDROXYL GROUP

    The hydroxyl derivatives of the paraffins are termed as alcohols.

    These are produced by the replacement of one, two or more hydrogen

    atoms of paraffins by the corresponding number of hydroxyl groups.

    Alcohols are monohydric, dihydric, trihydric or polhydric depending

    upon the number of hydroxyl groups present in them. Alcohol and

    hydroxyl compounds are generally liquids and soluble in water because

    of the functional group [IMAGE] which is capable of forming hydrogen

    bonds.

    Identification Tests

    A. ESTERIFICATION TEST

    Alcohols react with carboxylic acids in presence of a few drops of

    conc. H2SO4 as catalyst to form esters. A pleasant fruity smell

    indicates the presence of an ester and hence the hydroxyl group.

    Carboxylic Acid

    Ethyl Alcohol

    Ethyl ester

    [IMAGE]RCOOH + CH3CH2OH RCOOCH2CH3 + H2O

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    6. ALDEHYDE GROUP

    Identification Tests

    A. Fehling's solution Test

    Fehling's solution is an alkaline solution of copper sulphate

    containing sodium potassium tartrate (Rochelle salt) as the complexing

    agent.

    Being strong reducing agents, aldehydes reduce Fehling solution to red

    ppt. of cuprous oxide.

    [IMAGE]RCHO + 2Cu2+ + [IMAGE] [IMAGE] + Cu2O + 3H2O

    Aldehyde

    [IMAGE]

    B. Tollen's Reagent Test

    Tollen's reagent is an ammoniacal solution of silver nitrate and is

    prepared by adding NH4OH solution to AgNO3 solution till the ppts. Of

    Ag2O first formed just redissolve.

    When an aldehyde is heated with Tollen's reagent, the latter is

    reduced to metallic silver which deposits on the walls of the test

    tube.

    [IMAGE]2AgNO3 + 2NH4OH Ag2O + 2NH4NO3 + H2O

    Grey ppt.

    [IMAGE]Ag2O + 4NH4OH 2[Ag(NH3)2]+[IMAGE] + 3H2O

    Tollen's Reagent

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    C. Benedict's Test

    Benedict's solution is alkaline solution containing citrate ions as

    complexing agent.

    Aldehydes, on warming with this solution, give brick red precipitates.

    Red ppt.

    [IMAGE]RCHO + Cu2+ Cu2O + Oxidation Products

    O

    7. CARBOXYLIC GROUP

    [IMAGE]

    [IMAGE]

    [IMAGE][IMAGE]The organic compounds containing the carboxyl group ( C

    OH) as the functional group are called carboxylic acids. Acids

    containing one COOH group are termed monocarboxylic acids while others

    containing two or three COOH groups are called dicarboxylic and

    tricarboxylic respectively. These are normally soluble in water having

    pungent smell.

    Identification tests :

    A. Litmus Test

    Dissolve a pinch of the organic compound in water in a test tube and

    add to it 2-3 drops of the blue litmus solution. If the blue litmus

    solution turns red, it shows the presence of a carboxyl group.

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    B. ESTERIFICATION TEST

    Alcohols react with carboxylic acids in presence of a few drops of

    conc. H2SO4 as catalyst to form esters. A pleasant fruity smell

    indicates the presence of an ester and hence the carboxyl group.

    Carboxylic Acid

    Ethyl Alcohol

    Ethyl ester

    [IMAGE]RCOOH + CH3CH2COOH RCOOCH2CH3 + H2O

    C. Sodium Bicarbonate test

    Dissolve a pinch of the organic compound in water in a test tube . Add

    to it a few drops of aqueous NaHCO3 solution. If there is an

    effervescence due to the evolution of carbon dioxide, it shows the

    presence of a carboxyl group.

    [IMAGE]

    [IMAGE]RCOOH + NaHCO3 RCOONa +CO2 + H2O

    [IMAGE]

    [IMAGE]TEST FOR APPLE JUICE

    [IMAGE]

    1. CARBOHYDRATES

    a) Liquid + Fehling's Solution Red ppt. Present

    b) Liquid + Ammoniacal AgNO3 Silver mirror Present

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    c) Liquid + Benedict's solution Brick Red ppt. Present

    2. FATS AND OILS

    a) Liquid + CHCl3 + H2O Fat ppt. as emulsion Present

    b) Liquid + KHSO4 pungent irritating Present

    odour

    3. PROTEINS

    a) Liquid + Alkaline CuSO4 Violet ppt. Present

    b) Liquid + conc. HNO3 Yellow ppt. Present

    4. UNSATURATION

    a) Liquid + H2SO4 Clear solution Unsaturated

    5. HYDROXYL GROUP

    a) Liquid + C2H5OH+Conc. H2SO4 Fruity Smell Present

    6. ALDEHYDE

    a) Liquid + Fehling's Solution Red ppt. Present

    b) Liquid + Ammoniacal AgNO3 Silver mirror Present

    c) Liquid + Benedict's solution Brick Red ppt. Present

    7. CARBOXYLIC ACID

    a) Liquid + C2H5OH+Conc. H2SO4 Fruity Smell Present

    b) Liquid + Blue litmus solution Turns red Present

    c) Liquid + NaHCO3 solution Effervescence due Present

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    to evolution of CO2

    [IMAGE]Text Box: S.No. EXPERIMENT OBSERVATION INFERENCE

    [IMAGE]

    [IMAGE]TEST FOR COCUNUT WATER

    [IMAGE]

    1. CARBOHYDRATES

    a) Liquid + Fehling's Solution Red ppt. Present

    b) Liquid + Ammoniacal AgNO3 Silver mirror Present

    c) Liquid + Benedict's solution Brick Red ppt. Present

    2. FATS AND OILS

    a) Liquid + CHCl3 + H2O Fat ppt. as emulsion Present

    b) Liquid + KHSO4 pungent irritating Present

    odour

    3. PROTEINS

    a) Liquid + Alkaline CuSO4 No reaction Absent

    b) Liquid + conc. HNO3 Yellow ppt. Present

    4. UNSATURATION

    a) Liquid + H2SO4 Clear solution Unsaturated

    5. HYDROXYL GROUP

    a) Liquid + C2H5OH+Conc. H2SO4 Fruity Smell Present

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    6. ALDEHYDE

    a) Liquid + Fehling's Solution Red ppt. Present

    b) Liquid + Ammoniacal AgNO3 Silver mirror Present

    c) Liquid + Benedict's solution Brick Red ppt. Present

    7. CARBOXYLIC ACID

    a) Liquid + C2H5OH+Conc. H2SO4 Fruity Smell Present

    b) Liquid + Blue litmus solution Turns red Present

    c) Liquid + NaHCO3 solution Effervescence due Present

    to evolution of CO2

    [IMAGE]Text Box: S.No. EXPERIMENT OBSERVATION INFERENCE

    [IMAGE]TEST FOR TOMATO JUICE

    [IMAGE]

    1. CARBOHYDRATES

    a) Liquid + Fehling's Solution Red ppt. Present

    b) Liquid + Ammoniacal AgNO3 Silver mirror Present

    c) Liquid + Benedict's solution Brick Red ppt. Present

    2. FATS AND OILS

    a) Liquid + CHCl3 + H2O No reaction Absent

    b) Liquid + KHSO4 No reaction Absent

    3. PROTEINS

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    a) Liquid + Alkaline CuSO4 No reaction Absent

    b) Liquid + conc. HNO3 No reaction Absent

    4. UNSATURATION

    a) Liquid + H2SO4 Clear solution Unsaturated

    5. HYDROXYL GROUP

    a) Liquid + C2H5OH+Conc. H2SO4 Fruity Smell Present

    6. ALDEHYDE

    a) Liquid + Fehling's Solution Red ppt. Present

    b) Liquid + Ammoniacal AgNO3 Silver mirror Present

    c) Liquid + Benedict's solution Brick Red ppt. Present

    7. CARBOXYLIC ACID

    a) Liquid + C2H5OH+Conc. H2SO4 Fruity Smell Present

    b) Liquid + Blue litmus solution Turns red Present

    c) Liquid + NaHCO3 solution Effervescence due Present

    to evolution of CO2

    [IMAGE]Text Box: S.No. EXPERIMENT OBSERVATION INFERENCE

    RESULTS

    CONTENTS

    APPLE JUICE

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    COCUNUT WATER

    TOMATO JUICE

    CARBOHYDRATES

    YES

    YES

    YES

    PROTEINS

    YES

    NO

    NO

    FATS AND OILS

    YES

    YES

    NO

    UNSATURATION

    YES

    YES

    YES

    HYDROXYL GROUP

    YES

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    YES

    YES

    ALDEHYDE GROUP

    YES

    YES

    YES

    CARBOXYLIC GROUP

    YES

    YES

    YES

    CONCLUSION :

    After analyzing the fruits and vegetable juices, the following opinion

    emerges about the compounds present in them.

    CARBOHYDRATES : The carbohydrates are the main constituents of all the

    fruits and vegetable juices but are present in varying quantities.

    FATS AND OILS : Fats are rarely found in fruits and vegetables. But in

    the present study, the two juices (apple and coconut) show the

    presence of fats but it is not found in case of tomato.

    PROTEINS : Most of the fruits and vegetables contain proteins. In the

    present study, the coconut water and tomato juice did not show thepresence of proteins.

    UNSATURATION : Since each fruit and vegetable consists of

    carbohydrates, fats & proteins, the presence of double or triple bonds

    between carbon atoms is must. Therefore, almost all fruits and

    vegetables have unsaturated compounds.

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    HYDROXYL GROUP : This group is present in all the fruits and vegetable

     juices. But as compared to fruit juices, this group is present more in

    vegetable juices.

    ALDEHYDE GROUP : Aldehydes are type of carbohydrates. They are present

    in almost all the juices in large or small quantities.

    CARBOXYLIC ACID GROUP : It is also present in all the vegetables and

    fruit juices.

    How to Cite this Page

     AIM

    To analyse some fruits & vegetables juice for the contents present in them.

    INTRODUCTION

    Fruits and vegetable are always a part of balanced diet. That means fruits vegetables

    provide our body the essential nutrients, i.e. Carbohydrates, proteins, vitamins and

    minerals. Again their presence in these is being indicated by some of our general

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    observations, like freshly cut apples become reddish black after some time. !"planation

    for it is that iron present in apple gets o"idi"ed to iron o"ide. #o, we can conclude that

    fruits and vegetables contain comple" organic compounds, for e.g., anthocin,

    chlorophyll, esters$flavouring compounds%, carbohydrates, vitamins and can be tested in

    any fruits or vegetable by e"tracting out its juice and then subtracting it to various tests

     which are for detection of different classes of organic compounds. etection of minerals

    in vegetables or fruits means detection of elements other than carbon, hydrogen and

    o"ygen.

    MATERIAL REQUIRED

    • Test Tubes

    • 'urner

    • (itmus paper

    • (aboratory reagents

    •  )arious fruits

    •  )egetables juices

    CHEMICAL REQUIREMENTS

    • p* indicator

    • +odine solution

    Fehling solution A and Fehling solution '

    •  Ammonium chloride solution

    •  Ammonium hvdro"ide

    •  Ammonium o"alate

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    • otassium sulphocynaide solution

    PROCEDURE

    The juices are made dilute by adding distilled water to it, in order to remove colour and

    to make it colourless so that colour change can be easily watched and noted down. -ow 

    test for food components are taken down with the solution.

    TEST, OBSERVATION & INFERENCE

    Test Observation Inference

    ORANGE TEST:

    Test for acidity:

     Take 5ml of orange juice in a test tube anddip a pH paper in it. If pH is less than 7 the

     juice is acidic else the juice is basic.

     The pH comesout to be 6.

    Orange juice isacidic.

    Test for Startch:

     Take 2 ml of juice in a test tube and add fewdrops of iodine solution. It turns blue black

    in colour than the starch is present.

    bsence of blue black in

    colour.

    Orange juice isacidic.

    Test for Carbohydrates (FEHLING'S

    TEST):

     Take 2 ml of juice and ! ml of fehlingsolution " # and boil it. $ed precipitatesindicates the presence of producing sugarlike maltose% glucose % fructose " &actose.

    'o red colouredprecipitatesobtained.

    (arboh)dratesabsent.

    Test for Iro:

     Take 2 ml of juice add drop of conc. 'itricacid. #oil the solution cool and add 2*+

    drops of potassium sulphoc)anide

    solution .#lood red colours shows thepresence of iron.

    bsence of blood red

    colour.

    Iron is absent.

    Test for Ca!ci"#:

     Take 2 ml of juice add mmonium chlorideand ammonium h)dro,ide solution. -ilter

    the solution and to the lterate add 2 ml of mmonium O,alate solution. white ppt or

     /ellowprecipitate is

    obtained.

    (alcium ispresent.

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    milkiness indicates the presence of calcium.

    CONCLUSION

    From the table given behind it can be conducted that most of the fruits & vegetable

    contain carbohydrate & vegetable contain carbohydrate to a small e"tent. roteins are

    present in small uantity. Therefore one must not only depend on fruits and vegetables

    for a balance diet.

    '&/0I0

    O-

    13T#&0

    '4

    -$IT I(0

    !

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    ($TI-I(T

     T his is to certif) that

     

    of

    ( lass8 9II*

    H as successfull) completed this project report

     

    0I3'T$

    4T

    2

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    0.'o. (ontents

    II

    :age 'o.

    I.

    Introduction ;

    II.

    1.

    #ibliograph) >

    I'T$O4(TIO'

    +

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    -ruits and ?egetable are alwa)s a part of balanced diet. That

    means fruits ?egetables pro?ide our bod) the essential nutrients%

    i.e. (arboh)drates% proteins% ?itamins and minerals. gain their

    presence in these is being indicated b) some of our general

    obser?ations% like *freshl) cut apples become reddish black after

    some time. ,planation for it is that iron present in apple gets

    o,idised to iron o,ide. 0o% we can conclude that fruits and

    ?egetables contain comple, organic compounds% for e.g.% anthocin%

    chloroph)ll% esters@Aa?ouring compoundsB% carboh)drates%

    ?itamins and can be tested in an) fruits or ?egetable b) e,tracting

    out its juice and then subtracting it to ?arious tests which are for

    detection of diCerent classes of organic compounds. 4etection of 

    minerals in ?egetables or fruits means detection of elements other

    than carbon% h)drogen and o,)gen.

    ;

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    :$O(4$

     The juices are made dilute b) adding distilled water to it% in order

    to remo?e colour and to make it colourless so that colour change

    can be easil) watched and noted down. 'ow test for food

    components are taken down with the solution.

     T0T% O#0$1TIO' " I'-$'(

     Test Obser?ation Inference

    O$'3 T0T8

     Test for acidit)8

     Take 5ml of orange juice in a test

    tube and dip a pH paper in it. If pH is

    less than 7 the juice is acidic else the

     juice is basic.

     The pH

    comes out to

    be 6.Orange juice

    is acidic.

     Test for 0tarch8

     Take 2 ml of juice in a test tube and

    add few drops of iodine solution. It

    turns blue black in colour than the

    starch is present.

    bsence of 

    blue black in

    colour.Orange juice

    is acidic.

     Test for (arboh)drates

    @-H&I'3F0 T0TB8

     Take 2 ml of juice and ! ml of 

    -ehling solution " # and boil it.

    $ed precipitates indicates the

    presence of producing sugar like

    maltose% glucose % fructose " &actose.

    'o redcoloured

    precipitates

    obtained.

    (arboh)drate

    s absent.

     Test for Iron8

     Take 2 ml of juice add drop of conc.

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    'itric acid. #oil the solution cool and

    add 2*+ drops of potassium

    bsence of 

    blood red

    colour.

    Iron is

    absent.

    6

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    sulphoc)anide solution .#lood red

    colours shows the presence of iron.

     Test for (alcium8

     Take 2 ml of juice add mmonium

    chloride and ammonium h)dro,ide

    solution. -ilter the solution and to

    the ltrate add 2 ml of mmonium

    O,alate solution. white ppt or

    milkiness indicates the presence of 

    calcium.

     /ellow

    precipitate is

    obtained.

    (alcium is

    present.

    (O'(&0IO'7

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    -rom the table gi?en behind it can be conducted that most of the

    fruits " ?egetable contain carboh)drate " ?egetable contain

    carboh)drate to a small e,tent. :roteins are present in small

    =uantit). Therefore one must not onl) depend on fruits and

    ?egetables for a balance diet.

    #I#&IO3$:H/

    '($T (hemistr) :art ! " :art 2

    http8GGwww.wikipedia.org

    >

     AIM To anal)se some fruits " ?egetables juice for the contents present in them. 

    INTRODUCTION-ruits and ?egetable are alwa)s a part of balanced diet. That means fruits

    ?egetables pro?ide our bod) the essential nutrients% i.e. (arboh)drates% proteins%?itamins and minerals. gain their presence in these is being indicated b) some of

    our general obser?ations% like *freshl) cut apples become reddish black after some

    time. ,planation for it is that iron present in apple gets o,idi,ed to iron o,ide. 0o%we can conclude that fruits and ?egetables contain comple, organic compounds% for

    e.g.% anthocin% chloroph)ll% esters@Aa?ouring compoundsB% carboh)drates% ?itamins

    and can be tested in an) fruits or ?egetable b) e,tracting out its juice and then

    subtracting it to ?arious tests which are for detection of diCerent classes of organiccompounds. 4etection of minerals in ?egetables or fruits means detection of

    elements other than carbon% h)drogen and o,)gen. 

    COMPONENTS OF FOOD!. (arboh)drates8 (arboh)drates are pol)*h)dro,)alcohols% which ha?e an aldeh)deor ketones group. The) ha?e general formula (nH2nOn. (arboh)drates are the main

    source of energ) ! gm. of carboh)drates )ield !> of energ). The

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    monosaccharides ser?e as building block. 3lucose is also used in formation of fats "amino acids.

     

    2. Minerals: Minerals from 1-3% of the cell contents. Any marked change in the concentration

    results in the malfunctioning of cell & finally death. Some mineral present in the diet are:

    i! "alcium: #t is the ma$or component of one & teeth. "alcium is reuired for lood clotting'

    muscle contraction' ner(e impulse transmission & heart functioning. 

    ii! #ron: )aemogloin in our ody contains iron *hich is the uni(ersal carrier of +2 & ",2 '

    efficiency of iron causes anaemia due to failure of haemogloin synthesis.

    THE FRUITDevelopment of fruit: fter fertiliation the o?ar) also begin to grow and

    graduall) it matures into the fruit. The fruit ma)% therefore% be regarded as a matureor ripened o?ar). If% for some reason or other% fertiliation fails% o?ar) simpl) withers

    and falls oC. fruit consists of two portions% ?i. the per carp @peri% round8 karpos%

    fruitsB de?eloped from the wall of the o?ar)% and the seed de?eloped from theo?ules% apples% pineapples and some other fruits the o?ar) ma) grow into the fruit

    without fertiliation. 0uch a fruit is seedless or with immature seeds and is known as

    the parthenocarpic fruit. The pericarp ma) be thick or thin% when thick%it ma)

    consist of two or three parts8 the outer cell epicarp% from the skin of the fruitJ themiddle% called meson carp% is pulp) in fruits like mango% peach% plum etc. and the

    inner catted endocarp% is often ?er) think and membranous% as in orange% or it ma)

    be hard and ston) as in man) palms% mangoes% etc. In man) cases% howe?er% thepericarp is not diCerentiated into these three regions.

    Function of the fruit: he fruit gi(es protection to the seed and' therefore' to the emryo. #t

    stores food material. #t also helps in dispersal of the seed. ormally it is only the o(ary that

    gro*s into the fruit/ such a fruit is kno*n as the true fruit. Sometimes' ho*e(er' other floral

     parts' particularly the thalamus or e(en the caly0' may gro* and form a part of the fruit/ such a

    fruit is kno*n as the false fruit. "ommon e0amples of false fruits are apples' pear' cashe* nut'

    marking not' rose' dillenia' etc. #n dillenia' the caly0 ecomes thick and fleshy forming the only

    edile part of the fruit.

    MATERIAL REQUIRED•  Test Tubes

    • #urner

    • &itmus paper

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    • &aborator) reagents

    • 1arious fruits

    • 1egetables juices

     

    CHEMICAL REQUIREMENTS

    • pH indicator

    • Iodine solution

    • -ehling solution and -ehling solution #

    • mmonium chloride solution

    • mmonium h?dro,ide

    • mmonium o,alate

    • :otassium sulphoc)naide solution

    PROCEDURE The juices are made dilute b) adding distilled water to it% in order to remo?e colourand to make it colourless so that colour change can be easil) watched and noted

    down. 'ow test for food components are taken down with the solution. 

    TEST, OBSERVATION & INFERENCE

    Test  Observa

    tion  Inference

    ORANGE TEST:  

    Test for acidity:  

     Take 5ml of orange juice in atest tube and dip a pH

    paper in it. If pH is less than

    7 the juice is acidic else the

     juice is basic.

     The pHcomes

    out to be

    6.

    Orange

     juice is

    acidic.

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    Test for Startch:  

     Take 2 ml of juice in a test

    tube and add few drops of

    iodine solution. It turns blueblack in colour than the

    starch is present.

    bsence

    of blueblack in

    colour.

    Orange

     juice isacidic.

    Test for Carbohydrates

    (FEHLING'S TEST):

     Take 2 ml of juice and ! ml

    of fehling solution " # and

    boil it. $ed precipitatesindicates the presence of

    producing sugar like

    maltose% glucose % fructose

    " &actose.

    'o red

    colouredprecipitat

    es

    obtained.

    (arboh)drates

    absent.

    Test for Iro:  

     Take 2 ml of juice add drop

    of conc. 'itric acid. #oil the

    solution cool and add 2*+drops of potassium

    sulphoc)anide solution

    .#lood red colours showsthe presence of iron.

    bsenceof blood

    red

    colour.

    Iron is

    absent.

    Test for Ca!ci"#:  

     Take 2 ml of juice add

    mmonium chloride andammonium h)dro,ide

    solution. -ilter the solution

    and to the lterate add 2 ml

    of mmonium O,alatesolution. white ppt or

    milkiness indicates the

    presence of calcium.

     /ellow

    precipitat

    e isobtained.

    (alcium is

    present.

     TestObser?ati

    onInference

     TO

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     Test for acidit)8

     Take 5 ml of orange juice in

    a test tube and dip pHpaper in it. If pH is less than

    7 the juice is acidic and ifthe pH is more than 7 the

     juice is basic.

     The pHcomes

    out to be5.

     Tomato

     juice isacidic.

     Test for 0tartch8

     Take 2 ml of juice in a test

    tube and few drops of iodine

    solution. If blue black colourappears than starch is

    present.

    bsence

    of blueblack

    colour.

    0tarch ispresent.

     Test for (arboh)drates

    @-H&I'3F0 T0TB8

     Take 2 ml of juice and ! ml

    of fehling solution " # and

    boil it. $ed precipitates

    indicates the presence ofproducing sugar like

    maltose% glucose % fructose

    " &actose.

    bsence

    of red

    colourprecipitat

    es.

    (arboh)dr

    ates arenot present

    in tomato.

     Test for Iron8

     Take 2 ml of juice add drop

    of conc. 'itric acid boil the

    solution cool and add 2*+drops of potassium

    sulphoc)anide solution.

    #lood red colours shows thepresence of iron.

    :resence

    of bloodred

    colour.

    Iron ispresent.

     Test for (alcium8

     Take 2 ml of juice add

    mmonium chloride and

    ammonium h)dro,idesolution. -ilter the solution

    and to the lterate add 2 ml

    of mmonium O,alate

     /ellow

    precipitat

    esobtained.

    (alcium is

    present.

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    solution. Khite ppt ormilkiness indicates the

    presence of calcium.

     

    CONCLUSION-rom the table gi?en behind it can be conducted that most of the fruits " ?egetable

    contain carboh)drate " ?egetable contain carboh)drate to a small e,tent. :roteins

    are present in small =uantit). Therefore one must not onl) depend on fruits and?egetables for a balance diet.