From Eye to Insight ... DM750 M/Brochure… · 1 Considerations before cleaning Which image quality...
Transcript of From Eye to Insight ... DM750 M/Brochure… · 1 Considerations before cleaning Which image quality...
www.leica-microsystems.de
Order no.: English 11914709 ∙ Copyright © by Leica Microsystems CMS GmbH, W etzlar, Germany, 2018. Subject to modifications. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.
www.leica-microsystems.de
Order no.: English 11914709 ∙ Copyright © by Leica Microsystems CMS GmbH, W etzlar, Germany, 2018. Subject to modifications. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.
www.leica-microsystems.de
Order no.: English 11914709 ∙ Copyright © by Leica Microsystems CMS GmbH, W etzlar, Germany, 2018. Subject to modifications. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.
www.leica-microsystems.de
Order no.: English 11914709 ∙ Copyright © by Leica Microsystems CMS GmbH, W etzlar, Germany, 2018. Subject to modifications. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.
www.leica-microsystems.de
Order no.: English 11914709 ∙ Copyright © by Leica Microsystems CMS GmbH, W etzlar, Germany, 2018. Subject to modifications. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.
CLEANING OFMICROSCOPE OPTICS
From Eye to Insight
1 Considerations before cleaningWhich image quality do you expect?Optimize all microscope settings:
Aperture, Köhler, correction ring, etc.Check your specimen
I Use standard with proven qualityII Use cleaned specimen
Check slide and cover glassCheck immersion medium
I Same type as embedding medium of specimen II Is immersion medium from the same lot?
III Only use recommended typesIV No Anisol
2 Where to clean and where not
Where to clean1 Front lens of objective2 Cover glass of camera sensor3 Cover slip and specimen slide4 C-mount5 Condenser lens6 Misc. glass surfaces
Where not to cleanI Filter cubes
II All inside optics of a microscope
3 How to locate dirtTurn camera and inspect image
I If dirt is turning in the image ➞ not located in cameraII If dirt stays ➞ camera
Turn objective, condenser, C-mountI If dirt is turning with image ➞ objective, condenser, etc.
Control the relevant elements macroscopicallyI Magnifying glass or oculars upside down
Front lensI Turn it
II Look from the back against a bright background
4 Different kinds of dirtLoosely or not permanently attached dirt
I Glass (broken slides, cover slips, etc.)II Skin, dander
III Pollen, etc.Attached dirt
I Water solubleII Solvent soluble
III In practice often a mixture
5 How to cleanAim of cleaning
I Complete elimination of dust and dirt for perfectimage quality
II No remaining residues on the optical partsIII No damage of optical parts
General procedureI Locate dirt
II Inspect dirtIII Remove dustIV Remove water soluble dirt V Remove solvent soluble dirt
VI Inspect resultVII Repeat from III to VI if necessary
Remove dustI If possible do it manually
II Only use specified compressed aira Contamination with oil may cause
difficulties to remove residuesIII Perfect tool: bellows
Remove attached dirtI Never use rubbing materials, papers, microfibre cloths
II Never clean dryIII Always start to clean water soluble dirt
a If you can see grease start with solventsIV All solvents for cleaning should be absolutely clean
a PA solvents (ultra pure)b Destilled or demineralized waterc No ammonium containing glass cleaner like Sidolin,
Sparkle, etc.V All tools should be absolutely clean
a Use certified wound cotton sticks and dental cotton pads to avoid lint (no Q-tips or similar!)
Remove water soluble dirtI Use only clean, deionised water
a Simple method: breathe upon the surfaceII Use a cotton stick
a Clean from the centre toward the edge in concentric circles
III Inspect result
Remove solvent soluble dirtI Use ultra pure solvents
a Ethanol PAb Acetone PA (be careful, may harm plastics and
objective labels)II Use a cotton stick, dental cotton pad
a Cotton stick and cotton pad should only be moist, not dropping wet
b Clean from the centre toward the edge inconcentric circles
III Inspect resultIV Still smudgy?
a Combine the methods: breathe upon the surface and clean with cotton stick and acetone
Frontlens
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Leica Optics Cleaning Kit Order number 11505508